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The map label for this gene is azoR1 [H]

Identifier: 116492381

GI number: 116492381

Start: 642693

End: 643334

Strand: Direct

Name: azoR1 [H]

Synonym: PEPE_0586

Alternate gene names: 116492381

Gene position: 642693-643334 (Clockwise)

Preceding gene: 116492377

Following gene: 116492382

Centisome position: 35.07

GC content: 41.28

Gene sequence:

>642_bases
ATGAAAACATTAATTATTAACACCCATCCAGATTTTACTAACGAGGAACATTACTCCATTAAGCTCCAAAAGTTATTTAT
GGAGAAATTCCAGGAGAGTTTCCCCGCTGACCAACTAACACTTTTAAACCTTTATGATATCGAGGTCCCTCGCATTGAAA
AAGGCCAACTCATGGATGTTTGGCAAAAACAAATCGCGGGAGAACCACTATCAGCTACTGAACAAGCAGTTGCCGATCAA
TCAGCGCACTTACTGGCTCAATTTAAGGAACACCATCGGATTGTGATTGTGGCACCGCTCCATAACTTTAACGTCACGTC
CCGTATGAAAGACTATATGGATAATATTATGATTGCTCGGGAAACCTTTAAATACACCGAAGACGGATCCGTGGGATTGA
TGACCGATGATTATCGGGTGCTCTTACTCCAAGCTAGTGGCTCTATTTATACGAACCACGATCGTTATACACCTTTGGAA
TTTTCTCATTTCTATCTCCAAAAAATGTTTGAAGAAATCATGGGCTTTGATCAATTTTCAATTGTGCGGGCCCAAGGAAC
GTCCGTCATTACTCCTCAAGCCGTTTGGGCGAATGTTCCAGCTGATATGCAAAAAGCTTTTGATCAATTCTACGCTGACT
AA

Upstream 100 bases:

>100_bases
TGATTTTTTCAATTGCTAATCTCCTATTTAAAAGGTGCCATGTTTTGGCGCCTTTTTATTATATACTCGGTTTAACGAAG
TGAAAAGGGAGATTTTAAAG

Downstream 100 bases:

>100_bases
CTAGTAGTAGGCTACCGAGTTAAACCGATTCGAATATTGGATTAGATTAGAGTACAATATTCAAAAAGGAGGTAGCCCAT
GGAGCGTAAAAGACATGCTA

Product: Acyl carrier protein phosphodiesterase

Products: 4'- Phosphopantetheine; Apoprotein. [C]

Alternate protein names: Azo-dye reductase 1; FMN-dependent NADH-azo compound oxidoreductase 1 [H]

Number of amino acids: Translated: 213; Mature: 213

Protein sequence:

>213_residues
MKTLIINTHPDFTNEEHYSIKLQKLFMEKFQESFPADQLTLLNLYDIEVPRIEKGQLMDVWQKQIAGEPLSATEQAVADQ
SAHLLAQFKEHHRIVIVAPLHNFNVTSRMKDYMDNIMIARETFKYTEDGSVGLMTDDYRVLLLQASGSIYTNHDRYTPLE
FSHFYLQKMFEEIMGFDQFSIVRAQGTSVITPQAVWANVPADMQKAFDQFYAD

Sequences:

>Translated_213_residues
MKTLIINTHPDFTNEEHYSIKLQKLFMEKFQESFPADQLTLLNLYDIEVPRIEKGQLMDVWQKQIAGEPLSATEQAVADQ
SAHLLAQFKEHHRIVIVAPLHNFNVTSRMKDYMDNIMIARETFKYTEDGSVGLMTDDYRVLLLQASGSIYTNHDRYTPLE
FSHFYLQKMFEEIMGFDQFSIVRAQGTSVITPQAVWANVPADMQKAFDQFYAD
>Mature_213_residues
MKTLIINTHPDFTNEEHYSIKLQKLFMEKFQESFPADQLTLLNLYDIEVPRIEKGQLMDVWQKQIAGEPLSATEQAVADQ
SAHLLAQFKEHHRIVIVAPLHNFNVTSRMKDYMDNIMIARETFKYTEDGSVGLMTDDYRVLLLQASGSIYTNHDRYTPLE
FSHFYLQKMFEEIMGFDQFSIVRAQGTSVITPQAVWANVPADMQKAFDQFYAD

Specific function: Catalyzes the reductive cleavage of azo bond in aromatic azo compounds to the corresponding amines. Requires NADH, but not NADPH, as an electron donor for its activity [H]

COG id: COG1182

COG function: function code I; Acyl carrier protein phosphodiesterase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the azoreductase type 1 family [H]

Homologues:

Organism=Escherichia coli, GI1787680, Length=192, Percent_Identity=29.6875, Blast_Score=81, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003680
- InterPro:   IPR023048 [H]

Pfam domain/function: PF02525 Flavodoxin_2 [H]

EC number: 3.1.4.14 [C]

Molecular weight: Translated: 24725; Mature: 24725

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTLIINTHPDFTNEEHYSIKLQKLFMEKFQESFPADQLTLLNLYDIEVPRIEKGQLMDV
CCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCHHHH
WQKQIAGEPLSATEQAVADQSAHLLAQFKEHHRIVIVAPLHNFNVTSRMKDYMDNIMIAR
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHHHHHHH
ETFKYTEDGSVGLMTDDYRVLLLQASGSIYTNHDRYTPLEFSHFYLQKMFEEIMGFDQFS
HHHCCCCCCCEEEEECCEEEEEEEECCCEEECCCCCCCCHHHHHHHHHHHHHHHCCCCEE
IVRAQGTSVITPQAVWANVPADMQKAFDQFYAD
EEEECCCCEECCHHHHCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MKTLIINTHPDFTNEEHYSIKLQKLFMEKFQESFPADQLTLLNLYDIEVPRIEKGQLMDV
CCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCHHHH
WQKQIAGEPLSATEQAVADQSAHLLAQFKEHHRIVIVAPLHNFNVTSRMKDYMDNIMIAR
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHHHHHHH
ETFKYTEDGSVGLMTDDYRVLLLQASGSIYTNHDRYTPLEFSHFYLQKMFEEIMGFDQFS
HHHCCCCCCCEEEEECCEEEEEEEECCCEEECCCCCCCCHHHHHHHHHHHHHHHCCCCEE
IVRAQGTSVITPQAVWANVPADMQKAFDQFYAD
EEEECCCCEECCHHHHCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: [Acyl-Carrier Protein]; H2O [C]

Specific reaction: [Acyl-Carrier Protein] + H2O = 4'- Phosphopantetheine + Apoprotein. [C]

General reaction: Phosphoric ester hydrolysis [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12566566 [H]