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The map label for this gene is thiD [H]

Identifier: 116492354

GI number: 116492354

Start: 614651

End: 615466

Strand: Direct

Name: thiD [H]

Synonym: PEPE_0559

Alternate gene names: 116492354

Gene position: 614651-615466 (Clockwise)

Preceding gene: 116492353

Following gene: 116492355

Centisome position: 33.54

GC content: 48.28

Gene sequence:

>816_bases
ATGAAAAGTAAATTAGTACCTCAAGTTTTAACCATCGCCGGCACCGATAGTGGTGGGGGCGCCGGCATTCAAGCCGATAT
GAAGACGATGCAGGAGCGTCACGTTTTTTCGACCTGTGTGGTGGTGGCCGTTACCGCTCAAAACACGTTAGGCGTGCTCG
ATGTAGAAGTTATGCCCAAACGAATGATTGACCAACAATTTAAAGCCTTGGCGGAAGATCTAGATATCAAGGTAGCCAAG
ACTGGCATGCTCGCTAACACCGCGGTGGTAAACGCCGTGGTGGAAAACTATCGAAAATACGACTTTGGACCACTCGTCGT
CGATCCCGTCATGATTGCTAAAGGGGGCGCAAAGCTCCTGAGTGATGATGCGGTTGATGCCGTGCGTACCCAACTCGCAC
CGCTGGCCTACTTAATCATCCCCAACTTACCGGAAGTAGAAAAATTAGCTGGGATGGAAATCAAAAATAATGCCGATGTG
AAAGAGGCCGCTCGTCGCCTGCAACAACTCGGGGTTAAAAACGTCTTAATTAAGGGCGGTCATTTTAGTGATCAACCTGA
AAGTCGAGATTACGTCCTATTTGAAAATGGCGAAGAAATGGAATTGACGGGACCCCGTTTTGAAACGAATAACACCCATG
GCACAGGCGACACGATTTCTTCCGTGATTGTTGCCGAACTAGCTAAGGGAGCATCTTTGAAAGCAGCCTTGTGCACTGGT
AAAGCATTTATCAATGCGGCGATTAGTGATGGGATTCAAGTTGGCCATGGACATGGTCCCTTGAACCATTGGGCTTATGG
TGAGGAAGAACAATGA

Upstream 100 bases:

>100_bases
AAATCGACCAGGCAGTTTTTTGTCCTATCTTTTAGACGAATTAGCTAATATCACCCCTGAACAAGTTCAAGACCTTATGA
AAATTGGAGAAGCATAAATC

Downstream 100 bases:

>100_bases
AATTCAATTCAGATATGTTAAAAATTTATTTTGTGGCCGGCACCCAAGATGTTGCTAATAAGGCGGATTTCTTACCTAAA
GTGGAAGAAATCTTACAAGC

Product: hydroxymethylpyrimidine/phosphomethylpyrimidine kinase

Products: NA

Alternate protein names: Hydroxymethylpyrimidine kinase; HMP kinase; Hydroxymethylpyrimidine phosphate kinase; HMP-P kinase; HMP-phosphate kinase; HMPP kinase [H]

Number of amino acids: Translated: 271; Mature: 271

Protein sequence:

>271_residues
MKSKLVPQVLTIAGTDSGGGAGIQADMKTMQERHVFSTCVVVAVTAQNTLGVLDVEVMPKRMIDQQFKALAEDLDIKVAK
TGMLANTAVVNAVVENYRKYDFGPLVVDPVMIAKGGAKLLSDDAVDAVRTQLAPLAYLIIPNLPEVEKLAGMEIKNNADV
KEAARRLQQLGVKNVLIKGGHFSDQPESRDYVLFENGEEMELTGPRFETNNTHGTGDTISSVIVAELAKGASLKAALCTG
KAFINAAISDGIQVGHGHGPLNHWAYGEEEQ

Sequences:

>Translated_271_residues
MKSKLVPQVLTIAGTDSGGGAGIQADMKTMQERHVFSTCVVVAVTAQNTLGVLDVEVMPKRMIDQQFKALAEDLDIKVAK
TGMLANTAVVNAVVENYRKYDFGPLVVDPVMIAKGGAKLLSDDAVDAVRTQLAPLAYLIIPNLPEVEKLAGMEIKNNADV
KEAARRLQQLGVKNVLIKGGHFSDQPESRDYVLFENGEEMELTGPRFETNNTHGTGDTISSVIVAELAKGASLKAALCTG
KAFINAAISDGIQVGHGHGPLNHWAYGEEEQ
>Mature_271_residues
MKSKLVPQVLTIAGTDSGGGAGIQADMKTMQERHVFSTCVVVAVTAQNTLGVLDVEVMPKRMIDQQFKALAEDLDIKVAK
TGMLANTAVVNAVVENYRKYDFGPLVVDPVMIAKGGAKLLSDDAVDAVRTQLAPLAYLIIPNLPEVEKLAGMEIKNNADV
KEAARRLQQLGVKNVLIKGGHFSDQPESRDYVLFENGEEMELTGPRFETNNTHGTGDTISSVIVAELAKGASLKAALCTG
KAFINAAISDGIQVGHGHGPLNHWAYGEEEQ

Specific function: Catalyzes the phosphorylation of hydroxymethylpyrimidine phosphate (HMP-P) to HMP-PP, and of HMP to HMP-P [H]

COG id: COG0351

COG function: function code H; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiD family [H]

Homologues:

Organism=Escherichia coli, GI1788420, Length=257, Percent_Identity=39.2996108949416, Blast_Score=177, Evalue=8e-46,
Organism=Saccharomyces cerevisiae, GI6324517, Length=276, Percent_Identity=35.1449275362319, Blast_Score=132, Evalue=7e-32,
Organism=Saccharomyces cerevisiae, GI6324997, Length=272, Percent_Identity=34.9264705882353, Blast_Score=130, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6325378, Length=275, Percent_Identity=32.7272727272727, Blast_Score=130, Evalue=3e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013749
- InterPro:   IPR004399 [H]

Pfam domain/function: PF08543 Phos_pyr_kin [H]

EC number: =2.7.1.49; =2.7.4.7 [H]

Molecular weight: Translated: 28997; Mature: 28997

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSKLVPQVLTIAGTDSGGGAGIQADMKTMQERHVFSTCVVVAVTAQNTLGVLDVEVMPK
CCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHEEEEEEEECCCCCCEEEHHHHHH
RMIDQQFKALAEDLDIKVAKTGMLANTAVVNAVVENYRKYDFGPLVVDPVMIAKGGAKLL
HHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHHHCCCCCEEECCEEEECCCCHHH
SDDAVDAVRTQLAPLAYLIIPNLPEVEKLAGMEIKNNADVKEAARRLQQLGVKNVLIKGG
HHHHHHHHHHHHHHEEEEECCCCCCHHHHCCCCCCCCCCHHHHHHHHHHCCHHHEEEECC
HFSDQPESRDYVLFENGEEMELTGPRFETNNTHGTGDTISSVIVAELAKGASLKAALCTG
CCCCCCCCCCEEEEECCCEEEECCCEEECCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHH
KAFINAAISDGIQVGHGHGPLNHWAYGEEEQ
HHHHHHHHHCCEEECCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKSKLVPQVLTIAGTDSGGGAGIQADMKTMQERHVFSTCVVVAVTAQNTLGVLDVEVMPK
CCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHEEEEEEEECCCCCCEEEHHHHHH
RMIDQQFKALAEDLDIKVAKTGMLANTAVVNAVVENYRKYDFGPLVVDPVMIAKGGAKLL
HHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHHHCCCCCEEECCEEEECCCCHHH
SDDAVDAVRTQLAPLAYLIIPNLPEVEKLAGMEIKNNADVKEAARRLQQLGVKNVLIKGG
HHHHHHHHHHHHHHEEEEECCCCCCHHHHCCCCCCCCCCHHHHHHHHHHCCHHHEEEECC
HFSDQPESRDYVLFENGEEMELTGPRFETNNTHGTGDTISSVIVAELAKGASLKAALCTG
CCCCCCCCCCEEEEECCCEEEECCCEEECCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHH
KAFINAAISDGIQVGHGHGPLNHWAYGEEEQ
HHHHHHHHHCCEEECCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]