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The map label for this gene is thiE

Identifier: 116492355

GI number: 116492355

Start: 615463

End: 616113

Strand: Direct

Name: thiE

Synonym: PEPE_0560

Alternate gene names: 116492355

Gene position: 615463-616113 (Clockwise)

Preceding gene: 116492354

Following gene: 116492356

Centisome position: 33.59

GC content: 45.16

Gene sequence:

>651_bases
ATGAAATTCAATTCAGATATGTTAAAAATTTATTTTGTGGCCGGCACCCAAGATGTTGCTAATAAGGCGGATTTCTTACC
TAAAGTGGAAGAAATCTTACAAGCCGGGGCAACGGCCTTTCAACTACGGGAAAAGGGTTATAACAGTATCGATAATCCAG
CTGAATTAACCGAACTGGCTGAAAAATGCCATCAGTTAACCCAAAAGTATCACGTACCGTTGTTCATTGATGATGACGTG
GACTTAGCTTTAGCCATTCATGCTGAAGGCATTCACGTGGGTCAAAAAGATGAAAAAATTGAGTCAGTTTTAAAACGGGT
TCAGGGTTCGATGATTGTCGGGTTATCTTGTAACACCGAAGCGCAAGTCAAACAGGCTAACCAACTCGATGGAATTGATT
ATCTGGGAACCGGGACGGTCTTTGAAACGACCTCTAAAGCCGACGCGGGAGCCGCACTCGGTGTAGCCAAACTCCAAGAA
CTCGTCGAACTGAGTCACTATCCAATCGTCGCCATCGGGGGTATCACGCTTGATAATCTCCCCCAGACGATGGCTACGGG
AGTCAAAGGCTTTGCGGCCATTAGTATGTTTACGCAGATGACGGCAGTGCCCACTCAAATGCAAAAAATTAAAGCGATCG
TTAAGGGATGA

Upstream 100 bases:

>100_bases
GCACTGGTAAAGCATTTATCAATGCGGCGATTAGTGATGGGATTCAAGTTGGCCATGGACATGGTCCCTTGAACCATTGG
GCTTATGGTGAGGAAGAACA

Downstream 100 bases:

>100_bases
GGAGGAAAGCTATGTTTTCAGCACGTTTAAAAGCAATTGCTCAACCCGTCCTCGCCGATATTGAAAATCATCCATTCGTG
CGAGGAATTCAAGCGGGCGC

Product: thiamine monophosphate synthase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase

Number of amino acids: Translated: 216; Mature: 216

Protein sequence:

>216_residues
MKFNSDMLKIYFVAGTQDVANKADFLPKVEEILQAGATAFQLREKGYNSIDNPAELTELAEKCHQLTQKYHVPLFIDDDV
DLALAIHAEGIHVGQKDEKIESVLKRVQGSMIVGLSCNTEAQVKQANQLDGIDYLGTGTVFETTSKADAGAALGVAKLQE
LVELSHYPIVAIGGITLDNLPQTMATGVKGFAAISMFTQMTAVPTQMQKIKAIVKG

Sequences:

>Translated_216_residues
MKFNSDMLKIYFVAGTQDVANKADFLPKVEEILQAGATAFQLREKGYNSIDNPAELTELAEKCHQLTQKYHVPLFIDDDV
DLALAIHAEGIHVGQKDEKIESVLKRVQGSMIVGLSCNTEAQVKQANQLDGIDYLGTGTVFETTSKADAGAALGVAKLQE
LVELSHYPIVAIGGITLDNLPQTMATGVKGFAAISMFTQMTAVPTQMQKIKAIVKG
>Mature_216_residues
MKFNSDMLKIYFVAGTQDVANKADFLPKVEEILQAGATAFQLREKGYNSIDNPAELTELAEKCHQLTQKYHVPLFIDDDV
DLALAIHAEGIHVGQKDEKIESVLKRVQGSMIVGLSCNTEAQVKQANQLDGIDYLGTGTVFETTSKADAGAALGVAKLQE
LVELSHYPIVAIGGITLDNLPQTMATGVKGFAAISMFTQMTAVPTQMQKIKAIVKG

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP)

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family

Homologues:

Organism=Escherichia coli, GI1790426, Length=198, Percent_Identity=31.3131313131313, Blast_Score=81, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6325042, Length=209, Percent_Identity=31.5789473684211, Blast_Score=94, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIE_PEDPA (Q03GM4)

Other databases:

- EMBL:   CP000422
- RefSeq:   YP_804090.1
- ProteinModelPortal:   Q03GM4
- STRING:   Q03GM4
- GeneID:   4418250
- GenomeReviews:   CP000422_GR
- KEGG:   ppe:PEPE_0560
- NMPDR:   fig|278197.10.peg.513
- eggNOG:   COG0352
- HOGENOM:   HBG754477
- OMA:   ISCISAV
- PhylomeDB:   Q03GM4
- BioCyc:   PPEN278197:PEPE_0560-MONOMER
- HAMAP:   MF_00097
- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00693

Pfam domain/function: PF02581 TMP-TENI; SSF51391 TMP_synthase

EC number: =2.5.1.3

Molecular weight: Translated: 23326; Mature: 23326

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFNSDMLKIYFVAGTQDVANKADFLPKVEEILQAGATAFQLREKGYNSIDNPAELTELA
CCCCCCEEEEEEEECCHHHHHHHHCCHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHHHH
EKCHQLTQKYHVPLFIDDDVDLALAIHAEGIHVGQKDEKIESVLKRVQGSMIVGLSCNTE
HHHHHHHHHCCCCEEECCCCCEEEEEEECCCCCCCCHHHHHHHHHHHCCCEEEEEECCCH
AQVKQANQLDGIDYLGTGTVFETTSKADAGAALGVAKLQELVELSHYPIVAIGGITLDNL
HHHHHHHHCCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCC
PQTMATGVKGFAAISMFTQMTAVPTQMQKIKAIVKG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKFNSDMLKIYFVAGTQDVANKADFLPKVEEILQAGATAFQLREKGYNSIDNPAELTELA
CCCCCCEEEEEEEECCHHHHHHHHCCHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHHHH
EKCHQLTQKYHVPLFIDDDVDLALAIHAEGIHVGQKDEKIESVLKRVQGSMIVGLSCNTE
HHHHHHHHHCCCCEEECCCCCEEEEEEECCCCCCCCHHHHHHHHHHHCCCEEEEEECCCH
AQVKQANQLDGIDYLGTGTVFETTSKADAGAALGVAKLQELVELSHYPIVAIGGITLDNL
HHHHHHHHCCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCC
PQTMATGVKGFAAISMFTQMTAVPTQMQKIKAIVKG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA