Click here to switch to the map view.

The map label for this gene is thiM

Identifier: 116492353

GI number: 116492353

Start: 613850

End: 614647

Strand: Direct

Name: thiM

Synonym: PEPE_0558

Alternate gene names: 116492353

Gene position: 613850-614647 (Clockwise)

Preceding gene: 116492351

Following gene: 116492354

Centisome position: 33.5

GC content: 46.37

Gene sequence:

>798_bases
ATGAACCTTCAAGATATTGCCAAAATCCGGGAAGTTAATCCGGTAGTAGTTAATTATGCTAATTTTGTTACCCCATTTTT
GGTGGCGAATGGCTTAAATGCGGTGGGAGCTTCTCCCATCATGAGTGATGAAGTTAGTGAAGCGGAGGAACTCGTCCAAC
TCGCATCGGCCGTGGTGATTAATGCTGGCGCTTCTCGTAAAGAGAGCTGGCCACTGATGAATAAGCTGTGTGAAGCAGCT
AATCAATATCAAAAACCACTCGTCTTAGATCCCGTCGCAGTCGGTGCCACCCAGTACCGCAAAGCCCTCAATTTGATGTT
ACTAGAAAAATATCATTTCGATGTGATTCGTGGAAATATCGGAGAAATCGCCGTTTTAGCCGGTATCGATTGGCAAACGC
GCGGTATCGACGCTGGAAATGGGACCGGCGACATGAGCGCAGTTGTCCAAGCTTGTGCCCAGAAATTTAAGAACGTGGTG
ATTGCTAGTGGTGAAGTCGATTATATCAGCGACGGTCAACAAGTGATCACGGTGCACAATAACACTAAGTTGTTACCAGC
CATCGTGGGCTCCGGCGATTTATTATCCAGTATCGTAGGAGCGTTCAGCGCCGTAGCTGAAAATACTTTAGATGCTGCTG
TTACCGCTAGTTTGGTACTCTCGTGTGCGGGAGAACGTGCAGCGGCGCAACTCGATCAACAAAATCGACCAGGCAGTTTT
TTGTCCTATCTTTTAGACGAATTAGCTAATATCACCCCTGAACAAGTTCAAGACCTTATGAAAATTGGAGAAGCATAA

Upstream 100 bases:

>100_bases
GAAAGTGTTTAATTGTTGAAACCAAAACCATCCTTCTGTCTACAAAAGGGTGGTTTTTTCGACATTAAGGGCAGTGATAG
ATATTGGTAAGGAGCTTATT

Downstream 100 bases:

>100_bases
ATCATGAAAAGTAAATTAGTACCTCAAGTTTTAACCATCGCCGGCACCGATAGTGGTGGGGGCGCCGGCATTCAAGCCGA
TATGAAGACGATGCAGGAGC

Product: hydroxyethylthiazole kinase

Products: NA

Alternate protein names: 4-methyl-5-beta-hydroxyethylthiazole kinase; TH kinase; Thz kinase

Number of amino acids: Translated: 265; Mature: 265

Protein sequence:

>265_residues
MNLQDIAKIREVNPVVVNYANFVTPFLVANGLNAVGASPIMSDEVSEAEELVQLASAVVINAGASRKESWPLMNKLCEAA
NQYQKPLVLDPVAVGATQYRKALNLMLLEKYHFDVIRGNIGEIAVLAGIDWQTRGIDAGNGTGDMSAVVQACAQKFKNVV
IASGEVDYISDGQQVITVHNNTKLLPAIVGSGDLLSSIVGAFSAVAENTLDAAVTASLVLSCAGERAAAQLDQQNRPGSF
LSYLLDELANITPEQVQDLMKIGEA

Sequences:

>Translated_265_residues
MNLQDIAKIREVNPVVVNYANFVTPFLVANGLNAVGASPIMSDEVSEAEELVQLASAVVINAGASRKESWPLMNKLCEAA
NQYQKPLVLDPVAVGATQYRKALNLMLLEKYHFDVIRGNIGEIAVLAGIDWQTRGIDAGNGTGDMSAVVQACAQKFKNVV
IASGEVDYISDGQQVITVHNNTKLLPAIVGSGDLLSSIVGAFSAVAENTLDAAVTASLVLSCAGERAAAQLDQQNRPGSF
LSYLLDELANITPEQVQDLMKIGEA
>Mature_265_residues
MNLQDIAKIREVNPVVVNYANFVTPFLVANGLNAVGASPIMSDEVSEAEELVQLASAVVINAGASRKESWPLMNKLCEAA
NQYQKPLVLDPVAVGATQYRKALNLMLLEKYHFDVIRGNIGEIAVLAGIDWQTRGIDAGNGTGDMSAVVQACAQKFKNVV
IASGEVDYISDGQQVITVHNNTKLLPAIVGSGDLLSSIVGAFSAVAENTLDAAVTASLVLSCAGERAAAQLDQQNRPGSF
LSYLLDELANITPEQVQDLMKIGEA

Specific function: Thiamine biosynthesis. [C]

COG id: COG2145

COG function: function code H; Hydroxyethylthiazole kinase, sugar kinase family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Thz kinase family

Homologues:

Organism=Escherichia coli, GI1788421, Length=246, Percent_Identity=36.5853658536585, Blast_Score=146, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6325042, Length=277, Percent_Identity=28.158844765343, Blast_Score=88, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIM_PEDPA (Q03GM6)

Other databases:

- EMBL:   CP000422
- RefSeq:   YP_804088.1
- ProteinModelPortal:   Q03GM6
- STRING:   Q03GM6
- GeneID:   4418248
- GenomeReviews:   CP000422_GR
- KEGG:   ppe:PEPE_0558
- NMPDR:   fig|278197.10.peg.511
- eggNOG:   COG2145
- HOGENOM:   HBG351126
- OMA:   AIRGNAG
- PhylomeDB:   Q03GM6
- BioCyc:   PPEN278197:PEPE_0558-MONOMER
- HAMAP:   MF_00228
- InterPro:   IPR000417
- PANTHER:   PTHR20857:SF14
- PIRSF:   PIRSF000513
- PRINTS:   PR01099
- TIGRFAMs:   TIGR00694

Pfam domain/function: PF02110 HK

EC number: =2.7.1.50

Molecular weight: Translated: 28099; Mature: 28099

Theoretical pI: Translated: 4.30; Mature: 4.30

Prosite motif: NA

Important sites: BINDING 41-41 BINDING 117-117 BINDING 163-163 BINDING 190-190

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLQDIAKIREVNPVVVNYANFVTPFLVANGLNAVGASPIMSDEVSEAEELVQLASAVVI
CCHHHHHHHHHCCHHEEHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
NAGASRKESWPLMNKLCEAANQYQKPLVLDPVAVGATQYRKALNLMLLEKYHFDVIRGNI
CCCCCCCCCCHHHHHHHHHHHHHCCCEEECHHHHCHHHHHHHHHHHHHHHHHHHHHHCCC
GEIAVLAGIDWQTRGIDAGNGTGDMSAVVQACAQKFKNVVIASGEVDYISDGQQVITVHN
CCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCEEEECCCCCEECCCCEEEEEEC
NTKLLPAIVGSGDLLSSIVGAFSAVAENTLDAAVTASLVLSCAGERAAAQLDQQNRPGSF
CCEEEHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCHHH
LSYLLDELANITPEQVQDLMKIGEA
HHHHHHHHHCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MNLQDIAKIREVNPVVVNYANFVTPFLVANGLNAVGASPIMSDEVSEAEELVQLASAVVI
CCHHHHHHHHHCCHHEEHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
NAGASRKESWPLMNKLCEAANQYQKPLVLDPVAVGATQYRKALNLMLLEKYHFDVIRGNI
CCCCCCCCCCHHHHHHHHHHHHHCCCEEECHHHHCHHHHHHHHHHHHHHHHHHHHHHCCC
GEIAVLAGIDWQTRGIDAGNGTGDMSAVVQACAQKFKNVVIASGEVDYISDGQQVITVHN
CCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCEEEECCCCCEECCCCEEEEEEC
NTKLLPAIVGSGDLLSSIVGAFSAVAENTLDAAVTASLVLSCAGERAAAQLDQQNRPGSF
CCEEEHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCHHH
LSYLLDELANITPEQVQDLMKIGEA
HHHHHHHHHCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA