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The map label for this gene is rihA [H]

Identifier: 116492168

GI number: 116492168

Start: 411079

End: 412038

Strand: Reverse

Name: rihA [H]

Synonym: PEPE_0365

Alternate gene names: 116492168

Gene position: 412038-411079 (Counterclockwise)

Preceding gene: 116492170

Following gene: 116492167

Centisome position: 22.49

GC content: 41.04

Gene sequence:

>960_bases
TTGGCAACTAAAAAAATGATCTTAGATCTAGATACTGGTATTGATGATGCTATGGCCATTGCATATGCTTTGGCAGCACC
TGACGTTGATTTGATTGGTATTGTTTCGACATACGGGAATGTGTTAACCCCTGACGCTGGTACTAATAGCTTGAAAATTC
TTGAATTACTAGGCCGAACGGATATTCCGGTCTTTCTTGGTGAGGAACACTCACAAACTACCGAAAGCTTTGAAGTTATG
CCTATTAGTGCTCAAATTCATGGAAAAAATGGTATTGGTGAAGTTGAACTACCCGCTCCATCTCGGGCAATTGAAACACA
GCCCGGGGTTGATTTTATTATCGAAGCTGCTCACAAATACAACTCCGATTTAATTCTAGTGCCAACCGGTCCCCTAACAA
ATCTTGCACTTGCCTTAAAGAAAGATCCAGAAGTCGCCGATTTAATTGGCAACGTAACTATTATGGGTGGTGCTCTTACT
GTCCCTGGAAATGTTAGTCCAGTTGCAGAAGCTAACATCAACCAAGACCCTCACGCTGCTAATGACGTATTTACTAGCCA
CGTTAACTTAACCATGGTTGGTCTAGATGTCACTTTAAGAACGTTACTAACAAAAAAAGAAACACAACTTTGGCGCGACC
TAGATACTACTGCTGGTAGTAAATTTGCTGATATCGTCGATTACTATATCGACGTTTACAAAATTACCAGTCCTCATCTT
GGCGGTTGCGCCTTGCATGATCCTTTGGCGGTAGGAATTGCCCTTGATGCTTCATTTTCCAAAACTATGGATTTAAACAT
GTTCGTTGATACAACTGACGAAATGTGGGGTCGTACCATTGGTGATCCCGGCCGTTTAAACGAAGAAAATCCCAACGTTT
CCGTAGCTTTGAATGTCGATGCAGACTTTTATCTCAAGCGCTTTATGGATTATCTCACTAAATTATTTGCTAAAAATTAA

Upstream 100 bases:

>100_bases
TTCTTCAATAAAAAAGAATGCTTGGGATTGTCTTTTAAGCTTTGTTTACGTATGATTAAAGTAAGTTTAATACATAAATT
GTTTATTCGGAGGGGTTCTT

Downstream 100 bases:

>100_bases
TATCCTCTATACCAAAGCCACAAAAATCGATTTTTTTGTGGCTTTTTGGTACGCTTGGTTTAGAGAAACCAAAAAAGGAG
TCGTTGTGCATGTCCATTGT

Product: inosine-uridine nucleoside N-ribohydrolase

Products: D-ribose; purine

Alternate protein names: Cytidine/uridine-specific hydrolase [H]

Number of amino acids: Translated: 319; Mature: 318

Protein sequence:

>319_residues
MATKKMILDLDTGIDDAMAIAYALAAPDVDLIGIVSTYGNVLTPDAGTNSLKILELLGRTDIPVFLGEEHSQTTESFEVM
PISAQIHGKNGIGEVELPAPSRAIETQPGVDFIIEAAHKYNSDLILVPTGPLTNLALALKKDPEVADLIGNVTIMGGALT
VPGNVSPVAEANINQDPHAANDVFTSHVNLTMVGLDVTLRTLLTKKETQLWRDLDTTAGSKFADIVDYYIDVYKITSPHL
GGCALHDPLAVGIALDASFSKTMDLNMFVDTTDEMWGRTIGDPGRLNEENPNVSVALNVDADFYLKRFMDYLTKLFAKN

Sequences:

>Translated_319_residues
MATKKMILDLDTGIDDAMAIAYALAAPDVDLIGIVSTYGNVLTPDAGTNSLKILELLGRTDIPVFLGEEHSQTTESFEVM
PISAQIHGKNGIGEVELPAPSRAIETQPGVDFIIEAAHKYNSDLILVPTGPLTNLALALKKDPEVADLIGNVTIMGGALT
VPGNVSPVAEANINQDPHAANDVFTSHVNLTMVGLDVTLRTLLTKKETQLWRDLDTTAGSKFADIVDYYIDVYKITSPHL
GGCALHDPLAVGIALDASFSKTMDLNMFVDTTDEMWGRTIGDPGRLNEENPNVSVALNVDADFYLKRFMDYLTKLFAKN
>Mature_318_residues
ATKKMILDLDTGIDDAMAIAYALAAPDVDLIGIVSTYGNVLTPDAGTNSLKILELLGRTDIPVFLGEEHSQTTESFEVMP
ISAQIHGKNGIGEVELPAPSRAIETQPGVDFIIEAAHKYNSDLILVPTGPLTNLALALKKDPEVADLIGNVTIMGGALTV
PGNVSPVAEANINQDPHAANDVFTSHVNLTMVGLDVTLRTLLTKKETQLWRDLDTTAGSKFADIVDYYIDVYKITSPHLG
GCALHDPLAVGIALDASFSKTMDLNMFVDTTDEMWGRTIGDPGRLNEENPNVSVALNVDADFYLKRFMDYLTKLFAKN

Specific function: Hydrolyzes cytidine or uridine to ribose and cytosine or uracil, respectively [H]

COG id: COG1957

COG function: function code F; Inosine-uridine nucleoside N-ribohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IUNH family. RihA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786871, Length=313, Percent_Identity=32.9073482428115, Blast_Score=156, Evalue=2e-39,
Organism=Escherichia coli, GI1788486, Length=305, Percent_Identity=31.8032786885246, Blast_Score=144, Evalue=6e-36,
Organism=Escherichia coli, GI1786213, Length=313, Percent_Identity=30.3514376996805, Blast_Score=91, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI32565474, Length=207, Percent_Identity=33.3333333333333, Blast_Score=99, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17565698, Length=193, Percent_Identity=30.5699481865285, Blast_Score=73, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6320608, Length=327, Percent_Identity=26.2996941896024, Blast_Score=99, Evalue=8e-22,
Organism=Drosophila melanogaster, GI24583915, Length=191, Percent_Identity=29.8429319371728, Blast_Score=81, Evalue=9e-16,
Organism=Drosophila melanogaster, GI24641839, Length=191, Percent_Identity=30.8900523560209, Blast_Score=77, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015910
- InterPro:   IPR023186
- InterPro:   IPR001910
- InterPro:   IPR022975 [H]

Pfam domain/function: PF01156 IU_nuc_hydro [H]

EC number: 3.2.2.1

Molecular weight: Translated: 34401; Mature: 34269

Theoretical pI: Translated: 4.26; Mature: 4.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATKKMILDLDTGIDDAMAIAYALAAPDVDLIGIVSTYGNVLTPDAGTNSLKILELLGRT
CCCCEEEEEECCCCCHHHHHHHHHCCCCCCEEEEHHHCCCEECCCCCCCHHHHHHHHCCC
DIPVFLGEEHSQTTESFEVMPISAQIHGKNGIGEVELPAPSRAIETQPGVDFIIEAAHKY
CCCEEECCCCCCCCCCEEEEEEEEEEECCCCCCEEECCCCCCCCCCCCCHHHHHHHHHHC
NSDLILVPTGPLTNLALALKKDPEVADLIGNVTIMGGALTVPGNVSPVAEANINQDPHAA
CCCEEEEECCCHHHEEEEECCCCCHHHHHCCEEEECCEEECCCCCCCCCCCCCCCCCCCC
NDVFTSHVNLTMVGLDVTLRTLLTKKETQLWRDLDTTAGSKFADIVDYYIDVYKITSPHL
HHHEECCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHEEEEECCCCC
GGCALHDPLAVGIALDASFSKTMDLNMFVDTTDEMWGRTIGDPGRLNEENPNVSVALNVD
CCEECCCCEEEEEEEECCCCCEEEEEEEEECCHHHHCCCCCCCCCCCCCCCCEEEEEECC
ADFYLKRFMDYLTKLFAKN
HHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ATKKMILDLDTGIDDAMAIAYALAAPDVDLIGIVSTYGNVLTPDAGTNSLKILELLGRT
CCCEEEEEECCCCCHHHHHHHHHCCCCCCEEEEHHHCCCEECCCCCCCHHHHHHHHCCC
DIPVFLGEEHSQTTESFEVMPISAQIHGKNGIGEVELPAPSRAIETQPGVDFIIEAAHKY
CCCEEECCCCCCCCCCEEEEEEEEEEECCCCCCEEECCCCCCCCCCCCCHHHHHHHHHHC
NSDLILVPTGPLTNLALALKKDPEVADLIGNVTIMGGALTVPGNVSPVAEANINQDPHAA
CCCEEEEECCCHHHEEEEECCCCCHHHHHCCEEEECCEEECCCCCCCCCCCCCCCCCCCC
NDVFTSHVNLTMVGLDVTLRTLLTKKETQLWRDLDTTAGSKFADIVDYYIDVYKITSPHL
HHHEECCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHEEEEECCCCC
GGCALHDPLAVGIALDASFSKTMDLNMFVDTTDEMWGRTIGDPGRLNEENPNVSVALNVD
CCEECCCCEEEEEEEECCCCCEEEEEEEEECCHHHHCCCCCCCCCCCCCCCCEEEEEECC
ADFYLKRFMDYLTKLFAKN
HHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: N-D-ribosylpurine; H2O

Specific reaction: an N-D-ribosylpurine + H2O = D-ribose + a purine

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA