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The map label for this gene is manX [C]

Identifier: 116491988

GI number: 116491988

Start: 206497

End: 206919

Strand: Direct

Name: manX [C]

Synonym: PEPE_0174

Alternate gene names: 116491988

Gene position: 206497-206919 (Clockwise)

Preceding gene: 116491987

Following gene: 116491989

Centisome position: 11.27

GC content: 32.39

Gene sequence:

>423_bases
ATGACAAAAATAAATACAATTATTACGGGACATGGTAATTTCGCTTCTGGAATTGGGAGTTCACTTAAATTACTATCTGG
AGAACATGACGAAATTACATTAGTTGATTTCACTGAATCGATGAGTGAATTAGATTTGGGGGAGAAACTTGCTGACAGCT
TACAGCAAGATATACCAACGCTCTATTTTACTGATTTAGCCGGCGGAACACCTTTTAAAGAAGCTATAAAAATATCTATT
GATAAACCAGATGTTGAAGTTGTTTCAGGATGTAATTTGGCCGCATTGTTAGAGACTATGTTCAATGATTATGATAATGT
ACAAGCTTATGCTAAAGATTTAGTTGAAATAACTAAAAAAACAGCGGATTTAGCAGATTTTAGTACAAATATTGAAGAAG
AAAATATTGAAGATGGAATTTGA

Upstream 100 bases:

>100_bases
ATTTGTTGAAGAAAAAAGTATCGCCAATTGTTTTAATTGTAATAGTTTTTGTACTTTGTATTATATGTTCATACTTTAAG
ATTATGTAGAGGTATTAAAA

Downstream 100 bases:

>100_bases
TAATTAAGTTTAACCTTAAAATAAAAAGCTCTTAAGTGATGTGAAATCCCCATAATTATTAGATATTGTACTAATAATTA
TGGGGATTTTTTATGTAAGA

Product: phosphotransferase system, mannose/fructose-specific component IIA

Products: protein histidine; sugar phosphate

Alternate protein names: PTS System N-Acetylgalactosamine-Specific IIA Component; N-Acetylglucosamine/Galactosamine PTS EIIA

Number of amino acids: Translated: 140; Mature: 139

Protein sequence:

>140_residues
MTKINTIITGHGNFASGIGSSLKLLSGEHDEITLVDFTESMSELDLGEKLADSLQQDIPTLYFTDLAGGTPFKEAIKISI
DKPDVEVVSGCNLAALLETMFNDYDNVQAYAKDLVEITKKTADLADFSTNIEEENIEDGI

Sequences:

>Translated_140_residues
MTKINTIITGHGNFASGIGSSLKLLSGEHDEITLVDFTESMSELDLGEKLADSLQQDIPTLYFTDLAGGTPFKEAIKISI
DKPDVEVVSGCNLAALLETMFNDYDNVQAYAKDLVEITKKTADLADFSTNIEEENIEDGI
>Mature_139_residues
TKINTIITGHGNFASGIGSSLKLLSGEHDEITLVDFTESMSELDLGEKLADSLQQDIPTLYFTDLAGGTPFKEAIKISID
KPDVEVVSGCNLAALLETMFNDYDNVQAYAKDLVEITKKTADLADFSTNIEEENIEDGI

Specific function: This Is A Component Of The Phosphoenolpyruvate-Dependent Sugar Phosphotransferase System (Pts), A Major Carbohydrate Active -Transport System. The IIcd Domains Contain The Sugar Binding Site And The Transmembrane Channel; The IIa Domain Contains The Prim

COG id: COG2893

COG function: function code G; Phosphotransferase system, mannose/fructose-specific component IIA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: 220 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 2.7.1.69

Molecular weight: Translated: 15208; Mature: 15077

Theoretical pI: Translated: 3.86; Mature: 3.86

Prosite motif: PS51096 PTS_EIIA_TYPE_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKINTIITGHGNFASGIGSSLKLLSGEHDEITLVDFTESMSELDLGEKLADSLQQDIPT
CCCCCEEEECCCCHHHHCCCHHHHHCCCCCCEEEEEHHHHHHHCCHHHHHHHHHHHHCCE
LYFTDLAGGTPFKEAIKISIDKPDVEVVSGCNLAALLETMFNDYDNVQAYAKDLVEITKK
EEEECCCCCCCHHHHEEEECCCCCHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHH
TADLADFSTNIEEENIEDGI
HHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
TKINTIITGHGNFASGIGSSLKLLSGEHDEITLVDFTESMSELDLGEKLADSLQQDIPT
CCCCEEEECCCCHHHHCCCHHHHHCCCCCCEEEEEHHHHHHHCCHHHHHHHHHHHHCCE
LYFTDLAGGTPFKEAIKISIDKPDVEVVSGCNLAALLETMFNDYDNVQAYAKDLVEITKK
EEEECCCCCCCHHHHEEEECCCCCHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHH
TADLADFSTNIEEENIEDGI
HHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: Transferring phosphorus-containing groups; Phosphotransferases with an alcohol group as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA