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The map label for this gene is dapF [H]

Identifier: 116491952

GI number: 116491952

Start: 163810

End: 164799

Strand: Direct

Name: dapF [H]

Synonym: PEPE_0138

Alternate gene names: 116491952

Gene position: 163810-164799 (Clockwise)

Preceding gene: 116491951

Following gene: 116491953

Centisome position: 8.94

GC content: 38.99

Gene sequence:

>990_bases
ATGATTAAATTATTACGAGTACACGGATCAGAAAATTCATTTATATTACTTGATCAAGAACAACTCGAAATACCAATGAA
TCTAGACCAGTTAATTCAATTAGCAATTCAAGTTACTAATAATCAAACAGGGTTGTTAGGTGGGGCTGACGGACTTCTTG
TAGTTAGTTCAAGTGACCATGCTGGCGTTCGGGGACGAATGCAGGTTATTAATAACGACGGTAGCTTCGCTAGCATGTGT
GGAAATGGGCTGCGAACGGTGGCTCGTTATTTAGCCGAAAAATATGAAGAAAATGATTTTTTGGTCCAAACAGATCAAGC
CGATTTGCATGTAGGACGGGTAGCGACGATTGGAAATGGGTTACCCACATATCAGGTGGAAATTTCACCGGTTAGTTTTA
ATACTAAAAACCTACCAATGCAAATTGAAAATCAGGATAAATTGTTTAATGCGCCAGTTCGAGATTTTGATGATCAGCTT
CTTTTTTCAGCCGTGGCGGTACCTAATCCACATTTAATAGCAATTGATAATAATAACCGGCTTTCAACTAAAAAGTTAGC
GGAATTGGGTCATAGGTTAAATGCTTCCAATCCTTGGTTCCCAAAGGGAGTTAACGTGAGCTTTGGACGAGTCATTGGTC
AGAATCAATTGTTCGTCCAAACTTATGAACGGGGGGTTGGTTTTACTAATGCCTGTGGGACAGGGATGTCAGCGACAAGT
TTAGTTTGGAGCTTGTTAAATGATGGTCAAACTAGATTGGATGAAGTAAATGATATCTTTAATCCAGGTGGGATGGTCAA
AACTCGGGTAAATCAAATTCATGGTGCATATACGATTGATTTAATTGGGAATGCGACTTTTACCCACCAAATCTGGATTG
ATGAGGATCAATTGCGACAAAATAATTTGGAACAGGTTCAAATTGAATCAACTGGAGAAGAATTACATTACCAAGAGTTT
ATTCGACAACTACCTCGCTATCGCTTTTAG

Upstream 100 bases:

>100_bases
CTAGAATCTCAACGATGCTCGGAGTTCATCAAAACCAAGTAAATCAAGCTGTTCGTGAAATTTACAGAGAATTTTTTAAA
AACAAAGGTAGGGCAGACTG

Downstream 100 bases:

>100_bases
TGAAATAACTACTATATAATATAAAGATTTTCAGTTAACCTAATTTTCAGTAAAACTGAAACCCAAGCAGATTAATTTAG
GACTCTTTGGCTTTTTTACA

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase [H]

Number of amino acids: Translated: 329; Mature: 329

Protein sequence:

>329_residues
MIKLLRVHGSENSFILLDQEQLEIPMNLDQLIQLAIQVTNNQTGLLGGADGLLVVSSSDHAGVRGRMQVINNDGSFASMC
GNGLRTVARYLAEKYEENDFLVQTDQADLHVGRVATIGNGLPTYQVEISPVSFNTKNLPMQIENQDKLFNAPVRDFDDQL
LFSAVAVPNPHLIAIDNNNRLSTKKLAELGHRLNASNPWFPKGVNVSFGRVIGQNQLFVQTYERGVGFTNACGTGMSATS
LVWSLLNDGQTRLDEVNDIFNPGGMVKTRVNQIHGAYTIDLIGNATFTHQIWIDEDQLRQNNLEQVQIESTGEELHYQEF
IRQLPRYRF

Sequences:

>Translated_329_residues
MIKLLRVHGSENSFILLDQEQLEIPMNLDQLIQLAIQVTNNQTGLLGGADGLLVVSSSDHAGVRGRMQVINNDGSFASMC
GNGLRTVARYLAEKYEENDFLVQTDQADLHVGRVATIGNGLPTYQVEISPVSFNTKNLPMQIENQDKLFNAPVRDFDDQL
LFSAVAVPNPHLIAIDNNNRLSTKKLAELGHRLNASNPWFPKGVNVSFGRVIGQNQLFVQTYERGVGFTNACGTGMSATS
LVWSLLNDGQTRLDEVNDIFNPGGMVKTRVNQIHGAYTIDLIGNATFTHQIWIDEDQLRQNNLEQVQIESTGEELHYQEF
IRQLPRYRF
>Mature_329_residues
MIKLLRVHGSENSFILLDQEQLEIPMNLDQLIQLAIQVTNNQTGLLGGADGLLVVSSSDHAGVRGRMQVINNDGSFASMC
GNGLRTVARYLAEKYEENDFLVQTDQADLHVGRVATIGNGLPTYQVEISPVSFNTKNLPMQIENQDKLFNAPVRDFDDQL
LFSAVAVPNPHLIAIDNNNRLSTKKLAELGHRLNASNPWFPKGVNVSFGRVIGQNQLFVQTYERGVGFTNACGTGMSATS
LVWSLLNDGQTRLDEVNDIFNPGGMVKTRVNQIHGAYTIDLIGNATFTHQIWIDEDQLRQNNLEQVQIESTGEELHYQEF
IRQLPRYRF

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family [H]

Homologues:

Organism=Escherichia coli, GI87082334, Length=242, Percent_Identity=26.4462809917355, Blast_Score=79, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001653
- InterPro:   IPR018510 [H]

Pfam domain/function: PF01678 DAP_epimerase [H]

EC number: =5.1.1.7 [H]

Molecular weight: Translated: 36727; Mature: 36727

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS01326 DAP_EPIMERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKLLRVHGSENSFILLDQEQLEIPMNLDQLIQLAIQVTNNQTGLLGGADGLLVVSSSDH
CEEEEEEECCCCCEEEEEHHHCCCCCCHHHHHHHHEEECCCCCCEEECCCCEEEEECCCC
AGVRGRMQVINNDGSFASMCGNGLRTVARYLAEKYEENDFLVQTDQADLHVGRVATIGNG
CCCCEEEEEEECCCCCHHHHCCHHHHHHHHHHHHHCCCCCEEEECCCCEEECEEEEECCC
LPTYQVEISPVSFNTKNLPMQIENQDKLFNAPVRDFDDQLLFSAVAVPNPHLIAIDNNNR
CCEEEEEEEEEEECCCCCCEEECCCCCEECCCCCCCCHHHHHHHHCCCCCCEEEEECCCC
LSTKKLAELGHRLNASNPWFPKGVNVSFGRVIGQNQLFVQTYERGVGFTNACGTGMSATS
CHHHHHHHHHHHCCCCCCCCCCCCCCCHHHEECCCCEEEEEHHHCCCCCCCCCCCCHHHH
LVWSLLNDGQTRLDEVNDIFNPGGMVKTRVNQIHGAYTIDLIGNATFTHQIWIDEDQLRQ
HHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHCCEEEEEEECCCEEEEEEEECHHHHHH
NNLEQVQIESTGEELHYQEFIRQLPRYRF
CCCCEEEEECCCCHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MIKLLRVHGSENSFILLDQEQLEIPMNLDQLIQLAIQVTNNQTGLLGGADGLLVVSSSDH
CEEEEEEECCCCCEEEEEHHHCCCCCCHHHHHHHHEEECCCCCCEEECCCCEEEEECCCC
AGVRGRMQVINNDGSFASMCGNGLRTVARYLAEKYEENDFLVQTDQADLHVGRVATIGNG
CCCCEEEEEEECCCCCHHHHCCHHHHHHHHHHHHHCCCCCEEEECCCCEEECEEEEECCC
LPTYQVEISPVSFNTKNLPMQIENQDKLFNAPVRDFDDQLLFSAVAVPNPHLIAIDNNNR
CCEEEEEEEEEEECCCCCCEEECCCCCEECCCCCCCCHHHHHHHHCCCCCCEEEEECCCC
LSTKKLAELGHRLNASNPWFPKGVNVSFGRVIGQNQLFVQTYERGVGFTNACGTGMSATS
CHHHHHHHHHHHCCCCCCCCCCCCCCCHHHEECCCCEEEEEHHHCCCCCCCCCCCCHHHH
LVWSLLNDGQTRLDEVNDIFNPGGMVKTRVNQIHGAYTIDLIGNATFTHQIWIDEDQLRQ
HHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHCCEEEEEEECCCEEEEEEEECHHHHHH
NNLEQVQIESTGEELHYQEFIRQLPRYRF
CCCCEEEEECCCCHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12566566 [H]