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The map label for this gene is yclM [H]

Identifier: 116491951

GI number: 116491951

Start: 162431

End: 163810

Strand: Direct

Name: yclM [H]

Synonym: PEPE_0137

Alternate gene names: 116491951

Gene position: 162431-163810 (Clockwise)

Preceding gene: 116491942

Following gene: 116491952

Centisome position: 8.86

GC content: 39.86

Gene sequence:

>1380_bases
ATGGTAAAAGTTGTTAAGTTTGGTGGTAGCTCTCTAGCTAACGCAGCGCAATATCAAAAAGTAATTGAAATCTTAAGAGC
AGATCCCGAACGTCGAATTGTGGTCGTTTCAGCACCGGGGAAGCGTTTCAAAGATGATGTTAAGGTGACGGATCTATTAA
TCAAATATGCCAACGCATCTGGGGAGAACGTCAAAGTAGCACGTCAAGCAGTGCTAGACCGATATCAAGAAATTGCAGAT
GCATACCATGTTTCTGATTCGGATTTTGAACAAATCAATGTACATCTAATTGATTTAGCAAATTTGCACCACGCAAATAA
AGCATGCCGTCTTGCTCATTTTAAGGCGGCAGGGGAGCGGTTAAACGCAATTTTAATGGCATTAGTTTTAAAAAAATTCA
ATGTTACTGCGCGATTTGTTGATCCGAAACAATTGGGCATAAAGGCACATGGTGACTATAATAACGCGATCTTAGACCAG
CATTCATATGCTCAAATTGAGGCAGTTTGTTCAAATTTGCTGGATGAGGCGGAGTATCTTGTAGTTCCTGGTTTTTATGC
ATATAACCAAAATGGTGAGGTATGCACTTTCTCGCGGGGAGGGTCAGACATCACAGGGGCCATTCTCGCACGTGGTTTCC
AAGCTCAGCTTTATGAAAATTTTACGGATGTGGATTCCATTTATTCGGCTGACCCTAAAATCATTGCCCAGCCACATCCA
ATTCGAGAAATGACTTACCGTGAGATGCGGGAACTTGCATATGCTGGATTTTCCGTTTTTCACGATGAAGCCATCATCCC
AGCAATTGAAGCTCAAGTTCCAATTGTGATTAAAAACACGGATCATCCCAATTTACCAGGAACCGTAATTGAACCCGAAC
AAAATTATGCTTCTGATTTAGAAATAACAGGAGTGGCGAGCTCAAGTCGGTTTGCGGCTTTGTATATACACAAATATCTG
TTGAATCGTGAAGTCGGTTTTACCTTGAAATTATTAAAAATTTTATATCGTTACGATATTAGTTACGAACATATGCCTTC
TGGAATTGATGATTTAACAATTATTTTCAACCAGAATCAAATAACGGATGAAATTTTACCCAGTTTGTTATCCGATATTC
GGGAAGAATTGCACACTGACCAGTTAGAATGGATTGATGATTATGCAATCTTAATGGTGGTTGGAGAGGGAATGCGTGAC
AAGGTCGGCGTTGTAGATCGAATTGTGAATGCTCTAGCACGAAAACAAATTGGAATTCAGATGATTAACCAAGGGGCTTC
TAGAATCTCAACGATGCTCGGAGTTCATCAAAACCAAGTAAATCAAGCTGTTCGTGAAATTTACAGAGAATTTTTTAAAA
ACAAAGGTAGGGCAGACTGA

Upstream 100 bases:

>100_bases
CGATGTTAGTTAATTACTAGCGCCCATTTTCGTTTTTTTACGAATGGGCGTTTTTATTTTGGTTAGTTGGAAAGCTAGAA
CCTCGCAAAAGGAGACTAGT

Downstream 100 bases:

>100_bases
TGATTAAATTATTACGAGTACACGGATCAGAAAATTCATTTATATTACTTGATCAAGAACAACTCGAAATACCAATGAAT
CTAGACCAGTTAATTCAATT

Product: aspartate kinase

Products: NA

Alternate protein names: Aspartate kinase [H]

Number of amino acids: Translated: 459; Mature: 459

Protein sequence:

>459_residues
MVKVVKFGGSSLANAAQYQKVIEILRADPERRIVVVSAPGKRFKDDVKVTDLLIKYANASGENVKVARQAVLDRYQEIAD
AYHVSDSDFEQINVHLIDLANLHHANKACRLAHFKAAGERLNAILMALVLKKFNVTARFVDPKQLGIKAHGDYNNAILDQ
HSYAQIEAVCSNLLDEAEYLVVPGFYAYNQNGEVCTFSRGGSDITGAILARGFQAQLYENFTDVDSIYSADPKIIAQPHP
IREMTYREMRELAYAGFSVFHDEAIIPAIEAQVPIVIKNTDHPNLPGTVIEPEQNYASDLEITGVASSSRFAALYIHKYL
LNREVGFTLKLLKILYRYDISYEHMPSGIDDLTIIFNQNQITDEILPSLLSDIREELHTDQLEWIDDYAILMVVGEGMRD
KVGVVDRIVNALARKQIGIQMINQGASRISTMLGVHQNQVNQAVREIYREFFKNKGRAD

Sequences:

>Translated_459_residues
MVKVVKFGGSSLANAAQYQKVIEILRADPERRIVVVSAPGKRFKDDVKVTDLLIKYANASGENVKVARQAVLDRYQEIAD
AYHVSDSDFEQINVHLIDLANLHHANKACRLAHFKAAGERLNAILMALVLKKFNVTARFVDPKQLGIKAHGDYNNAILDQ
HSYAQIEAVCSNLLDEAEYLVVPGFYAYNQNGEVCTFSRGGSDITGAILARGFQAQLYENFTDVDSIYSADPKIIAQPHP
IREMTYREMRELAYAGFSVFHDEAIIPAIEAQVPIVIKNTDHPNLPGTVIEPEQNYASDLEITGVASSSRFAALYIHKYL
LNREVGFTLKLLKILYRYDISYEHMPSGIDDLTIIFNQNQITDEILPSLLSDIREELHTDQLEWIDDYAILMVVGEGMRD
KVGVVDRIVNALARKQIGIQMINQGASRISTMLGVHQNQVNQAVREIYREFFKNKGRAD
>Mature_459_residues
MVKVVKFGGSSLANAAQYQKVIEILRADPERRIVVVSAPGKRFKDDVKVTDLLIKYANASGENVKVARQAVLDRYQEIAD
AYHVSDSDFEQINVHLIDLANLHHANKACRLAHFKAAGERLNAILMALVLKKFNVTARFVDPKQLGIKAHGDYNNAILDQ
HSYAQIEAVCSNLLDEAEYLVVPGFYAYNQNGEVCTFSRGGSDITGAILARGFQAQLYENFTDVDSIYSADPKIIAQPHP
IREMTYREMRELAYAGFSVFHDEAIIPAIEAQVPIVIKNTDHPNLPGTVIEPEQNYASDLEITGVASSSRFAALYIHKYL
LNREVGFTLKLLKILYRYDISYEHMPSGIDDLTIIFNQNQITDEILPSLLSDIREELHTDQLEWIDDYAILMVVGEGMRD
KVGVVDRIVNALARKQIGIQMINQGASRISTMLGVHQNQVNQAVREIYREFFKNKGRAD

Specific function: Catalyzes 2 nonconsecutive reactions in the common biosynthetic pathway leading from Asp to diaminopimelate and Lys, to Met, and to Thr and Ile. [C]

COG id: COG0527

COG function: function code E; Aspartokinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ACT domains [H]

Homologues:

Organism=Escherichia coli, GI1786183, Length=467, Percent_Identity=27.4089935760171, Blast_Score=153, Evalue=2e-38,
Organism=Escherichia coli, GI1790455, Length=461, Percent_Identity=22.9934924078091, Blast_Score=95, Evalue=9e-21,
Organism=Escherichia coli, GI1790376, Length=301, Percent_Identity=26.578073089701, Blast_Score=67, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6320893, Length=471, Percent_Identity=21.8683651804671, Blast_Score=95, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR001048
- InterPro:   IPR001341
- InterPro:   IPR018042 [H]

Pfam domain/function: PF00696 AA_kinase; PF01842 ACT [H]

EC number: =2.7.2.4 [H]

Molecular weight: Translated: 51573; Mature: 51573

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: PS00324 ASPARTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKVVKFGGSSLANAAQYQKVIEILRADPERRIVVVSAPGKRFKDDVKVTDLLIKYANAS
CEEEEEECCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCC
GENVKVARQAVLDRYQEIADAYHVSDSDFEQINVHLIDLANLHHANKACRLAHFKAAGER
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEHHHHHHHHHHHHHHHHHHHHH
LNAILMALVLKKFNVTARFVDPKQLGIKAHGDYNNAILDQHSYAQIEAVCSNLLDEAEYL
HHHHHHHHHHHHHCCEEEEECHHHHCEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCEE
VVPGFYAYNQNGEVCTFSRGGSDITGAILARGFQAQLYENFTDVDSIYSADPKIIAQPHP
EECCEEEECCCCCEEEECCCCCCHHHHHHHCCHHHHHHHCHHHHHHHHCCCCCEEECCCC
IREMTYREMRELAYAGFSVFHDEAIIPAIEAQVPIVIKNTDHPNLPGTVIEPEQNYASDL
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCCCCCEECCCCCCCCCC
EITGVASSSRFAALYIHKYLLNREVGFTLKLLKILYRYDISYEHMPSGIDDLTIIFNQNQ
EEEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHCCCCCCCEEEEECCCC
ITDEILPSLLSDIREELHTDQLEWIDDYAILMVVGEGMRDKVGVVDRIVNALARKQIGIQ
CHHHHHHHHHHHHHHHHCCCHHHHHCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
MINQGASRISTMLGVHQNQVNQAVREIYREFFKNKGRAD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MVKVVKFGGSSLANAAQYQKVIEILRADPERRIVVVSAPGKRFKDDVKVTDLLIKYANAS
CEEEEEECCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCC
GENVKVARQAVLDRYQEIADAYHVSDSDFEQINVHLIDLANLHHANKACRLAHFKAAGER
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEHHHHHHHHHHHHHHHHHHHHH
LNAILMALVLKKFNVTARFVDPKQLGIKAHGDYNNAILDQHSYAQIEAVCSNLLDEAEYL
HHHHHHHHHHHHHCCEEEEECHHHHCEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCEE
VVPGFYAYNQNGEVCTFSRGGSDITGAILARGFQAQLYENFTDVDSIYSADPKIIAQPHP
EECCEEEECCCCCEEEECCCCCCHHHHHHHCCHHHHHHHCHHHHHHHHCCCCCEEECCCC
IREMTYREMRELAYAGFSVFHDEAIIPAIEAQVPIVIKNTDHPNLPGTVIEPEQNYASDL
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCCCCCEECCCCCCCCCC
EITGVASSSRFAALYIHKYLLNREVGFTLKLLKILYRYDISYEHMPSGIDDLTIIFNQNQ
EEEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHCCCCCCCEEEEECCCC
ITDEILPSLLSDIREELHTDQLEWIDDYAILMVVGEGMRDKVGVVDRIVNALARKQIGIQ
CHHHHHHHHHHHHHHHHCCCHHHHHCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
MINQGASRISTMLGVHQNQVNQAVREIYREFFKNKGRAD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969502; 9384377 [H]