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The map label for this gene is dapL

Identifier: 116491948

GI number: 116491948

Start: 158775

End: 159929

Strand: Reverse

Name: dapL

Synonym: PEPE_0134

Alternate gene names: 116491948

Gene position: 159929-158775 (Counterclockwise)

Preceding gene: 116491949

Following gene: 116491947

Centisome position: 8.73

GC content: 42.86

Gene sequence:

>1155_bases
ATGACCATTGAACCATTAAATTTAGTTCGAATAAGGCGGCAGTTTCACGAATCACCGGAATTAGCCCTAAAAGAATTCCA
GACCCACGCCCTACTTTTAAGCGTTATCCGCTCGCTACCGCAAAACTTGCTTGAAATTAAAACCATTCCCGAACTACCAA
CCGCTTTACTAGTTCGAGTTGCTGGTTCGCATCCTCATCGAACAATTGGATATCGAGCAGATATCGATGCACTTCCTGTC
AACGAAAAAACTGGCCTTGACTTCGCTTCCAAAACTCCAGGTATTATGCACGCTTGTGGTCATGATATTCACATGACGGT
TGCCTTGGGCATCTTAGAATATTTTGCTAACCATCAACCGCAAGATAATCTAATCTTCTTTTTCCAGCCAGCAGAAGAGA
GCCATAGTGGATCGGTTCGAGCTTTTAATGCAAATATTTTTACCAATCAGTTTAGACCAAACGAATTTTACGGGCTCCAT
AGTACGCCAACGTTGCCAGCTGGTGTAATTGGTTGCCGCATGGGAACCCTGTTTGCTGGAACTACAGAAGTTAATCTTAA
ATTAACCGGCAAAGGAGGACATGCAGCCTATCCACAAGACGCAAATGATATGGTTGTTGCTCAAGCTTATTTAATTACTC
AGCTTCAAACCATTGTTGCTCGTAATGTCAACCCCATCGAAGGTGGGGTTTTAACCCTTGGAAAAGTGTCTGCTGGAAAT
GTTCGAAATGTTATTGCTGATCAAGCCGTCATTGAGGGCACTATTCGCGGATTAACTCAGAAAATGATTCTTCTAATTCA
ACAACGCGTTCAACAAATTTGTGAAGGAACTGCCCAAGCCTTTAATTGCCAAGTCGAGGTCAAAATGAATCAAGGTGGGT
ATTTACCAGTGGAAAATGATCCAACCTTAACTCATGAATTAATTCAATTCATGCAAAGCGATTCGGCCATTAAGTTTAAA
AAAACTCCACCAGCAATGACCGGCGAAGATTTCGGATTCTTGCTGAGCAAGTTTCCAGGCACCATGTTTTGGTTGGGCGT
CGGCGCTACCAGTTCACTTCACTCTGCCACCTTTAATCCCGATGAACGTGCCATTCAATTGGGAATCGATGCAGTGATTA
AATTTCTTCAAAATCGAATGTCCAAGGGGGCTTAA

Upstream 100 bases:

>100_bases
GCTGGGGTACCAGCTCAAATTATTAAACGGGTTGATAATACAACTTCCCAGAAAACAGCGCTTGAAGACGGACTACGTAA
CCTATAAGGAAGTGTTTAAA

Downstream 100 bases:

>100_bases
ATATGCTTGAAAATGTTGATTTGATTTCCGCTATTATTACACCATTTGACGATCAGTTAAAAATTAACTTTACTGCGTTA
GAACGTTTAACTAATCATCT

Product: metal-dependent amidase/aminoacylase/carboxypeptidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 384; Mature: 383

Protein sequence:

>384_residues
MTIEPLNLVRIRRQFHESPELALKEFQTHALLLSVIRSLPQNLLEIKTIPELPTALLVRVAGSHPHRTIGYRADIDALPV
NEKTGLDFASKTPGIMHACGHDIHMTVALGILEYFANHQPQDNLIFFFQPAEESHSGSVRAFNANIFTNQFRPNEFYGLH
STPTLPAGVIGCRMGTLFAGTTEVNLKLTGKGGHAAYPQDANDMVVAQAYLITQLQTIVARNVNPIEGGVLTLGKVSAGN
VRNVIADQAVIEGTIRGLTQKMILLIQQRVQQICEGTAQAFNCQVEVKMNQGGYLPVENDPTLTHELIQFMQSDSAIKFK
KTPPAMTGEDFGFLLSKFPGTMFWLGVGATSSLHSATFNPDERAIQLGIDAVIKFLQNRMSKGA

Sequences:

>Translated_384_residues
MTIEPLNLVRIRRQFHESPELALKEFQTHALLLSVIRSLPQNLLEIKTIPELPTALLVRVAGSHPHRTIGYRADIDALPV
NEKTGLDFASKTPGIMHACGHDIHMTVALGILEYFANHQPQDNLIFFFQPAEESHSGSVRAFNANIFTNQFRPNEFYGLH
STPTLPAGVIGCRMGTLFAGTTEVNLKLTGKGGHAAYPQDANDMVVAQAYLITQLQTIVARNVNPIEGGVLTLGKVSAGN
VRNVIADQAVIEGTIRGLTQKMILLIQQRVQQICEGTAQAFNCQVEVKMNQGGYLPVENDPTLTHELIQFMQSDSAIKFK
KTPPAMTGEDFGFLLSKFPGTMFWLGVGATSSLHSATFNPDERAIQLGIDAVIKFLQNRMSKGA
>Mature_383_residues
TIEPLNLVRIRRQFHESPELALKEFQTHALLLSVIRSLPQNLLEIKTIPELPTALLVRVAGSHPHRTIGYRADIDALPVN
EKTGLDFASKTPGIMHACGHDIHMTVALGILEYFANHQPQDNLIFFFQPAEESHSGSVRAFNANIFTNQFRPNEFYGLHS
TPTLPAGVIGCRMGTLFAGTTEVNLKLTGKGGHAAYPQDANDMVVAQAYLITQLQTIVARNVNPIEGGVLTLGKVSAGNV
RNVIADQAVIEGTIRGLTQKMILLIQQRVQQICEGTAQAFNCQVEVKMNQGGYLPVENDPTLTHELIQFMQSDSAIKFKK
TPPAMTGEDFGFLLSKFPGTMFWLGVGATSSLHSATFNPDERAIQLGIDAVIKFLQNRMSKGA

Specific function: Catalyzes the conversion of N-acetyl-diaminopimelate to diaminopimelate and acetate

COG id: COG1473

COG function: function code R; Metal-dependent amidase/aminoacylase/carboxypeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapL subfamily

Homologues:

Organism=Escherichia coli, GI87081880, Length=425, Percent_Identity=22.3529411764706, Blast_Score=82, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPL_PEDPA (Q03HT1)

Other databases:

- EMBL:   CP000422
- RefSeq:   YP_803683.1
- ProteinModelPortal:   Q03HT1
- SMR:   Q03HT1
- STRING:   Q03HT1
- GeneID:   4417132
- GenomeReviews:   CP000422_GR
- KEGG:   ppe:PEPE_0134
- NMPDR:   fig|278197.10.peg.118
- eggNOG:   COG1473
- HOGENOM:   HBG708500
- OMA:   NQGGYLP
- ProtClustDB:   CLSK936799
- BioCyc:   PPEN278197:PEPE_0134-MONOMER
- HAMAP:   MF_01692
- InterPro:   IPR017439
- InterPro:   IPR010168
- InterPro:   IPR002933
- InterPro:   IPR011650
- PIRSF:   PIRSF005962
- TIGRFAMs:   TIGR01891

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20; SSF55031 Peptidase_M20_dimer

EC number: =3.5.1.47

Molecular weight: Translated: 42083; Mature: 41952

Theoretical pI: Translated: 7.19; Mature: 7.19

Prosite motif: PS00758 ARGE_DAPE_CPG2_1; PS00759 ARGE_DAPE_CPG2_2; PS00107 PROTEIN_KINASE_ATP

Important sites: ACT_SITE 74-74 ACT_SITE 133-133

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIEPLNLVRIRRQFHESPELALKEFQTHALLLSVIRSLPQNLLEIKTIPELPTALLVRV
CCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
AGSHPHRTIGYRADIDALPVNEKTGLDFASKTPGIMHACGHDIHMTVALGILEYFANHQP
CCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHCCCC
QDNLIFFFQPAEESHSGSVRAFNANIFTNQFRPNEFYGLHSTPTLPAGVIGCRMGTLFAG
CCCEEEEEECCCCCCCCCEEEEEEEEEECCCCCCCEECCCCCCCCCHHHHHHHHHHEEEC
TTEVNLKLTGKGGHAAYPQDANDMVVAQAYLITQLQTIVARNVNPIEGGVLTLGKVSAGN
CEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCC
VRNVIADQAVIEGTIRGLTQKMILLIQQRVQQICEGTAQAFNCQVEVKMNQGGYLPVEND
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEECCCCEECCCCC
PTLTHELIQFMQSDSAIKFKKTPPAMTGEDFGFLLSKFPGTMFWLGVGATSSLHSATFNP
CCHHHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHCCCCEEEEECCCCCCCCCCCCCC
DERAIQLGIDAVIKFLQNRMSKGA
CHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TIEPLNLVRIRRQFHESPELALKEFQTHALLLSVIRSLPQNLLEIKTIPELPTALLVRV
CCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
AGSHPHRTIGYRADIDALPVNEKTGLDFASKTPGIMHACGHDIHMTVALGILEYFANHQP
CCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHCCCC
QDNLIFFFQPAEESHSGSVRAFNANIFTNQFRPNEFYGLHSTPTLPAGVIGCRMGTLFAG
CCCEEEEEECCCCCCCCCEEEEEEEEEECCCCCCCEECCCCCCCCCHHHHHHHHHHEEEC
TTEVNLKLTGKGGHAAYPQDANDMVVAQAYLITQLQTIVARNVNPIEGGVLTLGKVSAGN
CEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCC
VRNVIADQAVIEGTIRGLTQKMILLIQQRVQQICEGTAQAFNCQVEVKMNQGGYLPVEND
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEECCCCEECCCCC
PTLTHELIQFMQSDSAIKFKKTPPAMTGEDFGFLLSKFPGTMFWLGVGATSSLHSATFNP
CCHHHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHCCCCEEEEECCCCCCCCCCCCCC
DERAIQLGIDAVIKFLQNRMSKGA
CHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA