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The map label for this gene is dapA

Identifier: 116491947

GI number: 116491947

Start: 157873

End: 158772

Strand: Reverse

Name: dapA

Synonym: PEPE_0133

Alternate gene names: 116491947

Gene position: 158772-157873 (Counterclockwise)

Preceding gene: 116491948

Following gene: 116491946

Centisome position: 8.66

GC content: 42.0

Gene sequence:

>900_bases
ATGCTTGAAAATGTTGATTTGATTTCCGCTATTATTACACCATTTGACGATCAGTTAAAAATTAACTTTACTGCGTTAGA
ACGTTTAACTAATCATCTCATTGAAACCGGCAATACTGGTTTTATTATCGGGGGAACTACAGGTGAGACACCTACCTTAA
CTCACGCTGAAAAATTAGGTCTGTATACTCGCTTTGCTGAAATAGTCGCTGGACGAGTACCCATCATTGCTGGAACCGGA
TCAAATAATACCCAAGCTACCATTGACTTCACTAAAGAAGTTCGCCAAATTGACGGGATCATTGCCGCTCTAGTCGTTAC
CCCCTACTACAATAAGCCTAATCAACGCGGGATGGTAGCGCACTTTAAAACCGTCGCGCGCCAAGCTGACTTTCCGATTA
TGATGTATAACATTCCCGGACGAACCGGTGTTCAAATGGAAAATGCAACCATTGTTGAGCTTTCTCAAGAACCAAATATC
ATTGGAATTAAACAGTGTACAAATTTAAATGACATTGGTTTTTTAGTTGAAAACACCCCCAATGATTTTGCTGTATATAC
CGGAAATGATCCAGAAACTCTGGGTGCAGTCGCTTTAGGCGCAAATGGGGTTGCTTCGGTTGCAAGCCACATATATGGCT
ATCAGATACGAGCACTTTTAGATGCTGTGAAACACGGGGATCTATTAAAAGCCGGAAAATTACAGCGAGAATTAACACCC
AAAATGGAAGCCCTCTTCTTATATCCGTCACCGGCACCAGTTAAAGCCGTTCTAAACGCTCAAAATTGGTCCGTTGGTAG
TCCACGACTTCCTATCTTGCCATTAAATCAAAAAGAAAAGCTCAATTTAGCCCATCAGCTGGGGGTTAATCATCTTGCCG
ACGTGCAGTTGCATTACTAG

Upstream 100 bases:

>100_bases
TTCACTCTGCCACCTTTAATCCCGATGAACGTGCCATTCAATTGGGAATCGATGCAGTGATTAAATTTCTTCAAAATCGA
ATGTCCAAGGGGGCTTAAAT

Downstream 100 bases:

>100_bases
GAAAGGAGCCATTATATGATAAATCAGGTTAAAACTGTTGTGATTGCTGGTTGTACAGGATCTATGGGTCAACAAACCGT
TCAGTTAATCCAAAACTTAC

Product: dihydrodipicolinate synthase

Products: NA

Alternate protein names: DHDPS

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MLENVDLISAIITPFDDQLKINFTALERLTNHLIETGNTGFIIGGTTGETPTLTHAEKLGLYTRFAEIVAGRVPIIAGTG
SNNTQATIDFTKEVRQIDGIIAALVVTPYYNKPNQRGMVAHFKTVARQADFPIMMYNIPGRTGVQMENATIVELSQEPNI
IGIKQCTNLNDIGFLVENTPNDFAVYTGNDPETLGAVALGANGVASVASHIYGYQIRALLDAVKHGDLLKAGKLQRELTP
KMEALFLYPSPAPVKAVLNAQNWSVGSPRLPILPLNQKEKLNLAHQLGVNHLADVQLHY

Sequences:

>Translated_299_residues
MLENVDLISAIITPFDDQLKINFTALERLTNHLIETGNTGFIIGGTTGETPTLTHAEKLGLYTRFAEIVAGRVPIIAGTG
SNNTQATIDFTKEVRQIDGIIAALVVTPYYNKPNQRGMVAHFKTVARQADFPIMMYNIPGRTGVQMENATIVELSQEPNI
IGIKQCTNLNDIGFLVENTPNDFAVYTGNDPETLGAVALGANGVASVASHIYGYQIRALLDAVKHGDLLKAGKLQRELTP
KMEALFLYPSPAPVKAVLNAQNWSVGSPRLPILPLNQKEKLNLAHQLGVNHLADVQLHY
>Mature_299_residues
MLENVDLISAIITPFDDQLKINFTALERLTNHLIETGNTGFIIGGTTGETPTLTHAEKLGLYTRFAEIVAGRVPIIAGTG
SNNTQATIDFTKEVRQIDGIIAALVVTPYYNKPNQRGMVAHFKTVARQADFPIMMYNIPGRTGVQMENATIVELSQEPNI
IGIKQCTNLNDIGFLVENTPNDFAVYTGNDPETLGAVALGANGVASVASHIYGYQIRALLDAVKHGDLLKAGKLQRELTP
KMEALFLYPSPAPVKAVLNAQNWSVGSPRLPILPLNQKEKLNLAHQLGVNHLADVQLHY

Specific function: Biosynthesis of diaminopimelate and lysine from aspartate semialdehyde; first step. [C]

COG id: COG0329

COG function: function code EM; Dihydrodipicolinate synthase/N-acetylneuraminate lyase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DHDPS family

Homologues:

Organism=Homo sapiens, GI31543060, Length=274, Percent_Identity=29.1970802919708, Blast_Score=119, Evalue=4e-27,
Organism=Homo sapiens, GI13540533, Length=232, Percent_Identity=27.1551724137931, Blast_Score=75, Evalue=6e-14,
Organism=Escherichia coli, GI1788823, Length=275, Percent_Identity=33.4545454545455, Blast_Score=174, Evalue=5e-45,
Organism=Escherichia coli, GI1789620, Length=287, Percent_Identity=28.2229965156794, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI87082415, Length=274, Percent_Identity=27.007299270073, Blast_Score=101, Evalue=7e-23,
Organism=Escherichia coli, GI1786463, Length=249, Percent_Identity=32.9317269076305, Blast_Score=94, Evalue=8e-21,

Paralogues:

None

Copy number: 840 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): DAPA_PEDPA (Q03HT2)

Other databases:

- EMBL:   CP000422
- RefSeq:   YP_803682.1
- ProteinModelPortal:   Q03HT2
- SMR:   Q03HT2
- STRING:   Q03HT2
- GeneID:   4417131
- GenomeReviews:   CP000422_GR
- KEGG:   ppe:PEPE_0133
- NMPDR:   fig|278197.10.peg.117
- eggNOG:   COG0329
- HOGENOM:   HBG358848
- OMA:   TVEFAKG
- ProtClustDB:   PRK03170
- BioCyc:   PPEN278197:PEPE_0133-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00418
- InterPro:   IPR013785
- InterPro:   IPR005263
- InterPro:   IPR002220
- InterPro:   IPR020625
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR12128
- PRINTS:   PR00146
- TIGRFAMs:   TIGR00674

Pfam domain/function: PF00701 DHDPS

EC number: =4.2.1.52

Molecular weight: Translated: 32535; Mature: 32535

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00665 DHDPS_1; PS00666 DHDPS_2

Important sites: ACT_SITE 164-164 BINDING 109-109

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLENVDLISAIITPFDDQLKINFTALERLTNHLIETGNTGFIIGGTTGETPTLTHAEKLG
CCCCHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHH
LYTRFAEIVAGRVPIIAGTGSNNTQATIDFTKEVRQIDGIIAALVVTPYYNKPNQRGMVA
HHHHHHHHHCCCCCEEEECCCCCCEEEEEHHHHHHHHHHHHEEEEEECCCCCCCCCCHHH
HFKTVARQADFPIMMYNIPGRTGVQMENATIVELSQEPNIIGIKQCTNLNDIGFLVENTP
HHHHHHHCCCCCEEEEECCCCCCCEECCEEEEEECCCCCEEEEHHCCCCCCCEEEEECCC
NDFAVYTGNDPETLGAVALGANGVASVASHIYGYQIRALLDAVKHGDLLKAGKLQRELTP
CCEEEEECCCCHHHEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCC
KMEALFLYPSPAPVKAVLNAQNWSVGSPRLPILPLNQKEKLNLAHQLGVNHLADVQLHY
CEEEEEECCCCCCCEEEEECCCCCCCCCCCCEEECCCHHHHHHHHHHCCCCEEEEEEEC
>Mature Secondary Structure
MLENVDLISAIITPFDDQLKINFTALERLTNHLIETGNTGFIIGGTTGETPTLTHAEKLG
CCCCHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHH
LYTRFAEIVAGRVPIIAGTGSNNTQATIDFTKEVRQIDGIIAALVVTPYYNKPNQRGMVA
HHHHHHHHHCCCCCEEEECCCCCCEEEEEHHHHHHHHHHHHEEEEEECCCCCCCCCCHHH
HFKTVARQADFPIMMYNIPGRTGVQMENATIVELSQEPNIIGIKQCTNLNDIGFLVENTP
HHHHHHHCCCCCEEEEECCCCCCCEECCEEEEEECCCCCEEEEHHCCCCCCCEEEEECCC
NDFAVYTGNDPETLGAVALGANGVASVASHIYGYQIRALLDAVKHGDLLKAGKLQRELTP
CCEEEEECCCCHHHEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCC
KMEALFLYPSPAPVKAVLNAQNWSVGSPRLPILPLNQKEKLNLAHQLGVNHLADVQLHY
CEEEEEECCCCCCCEEEEECCCCCCCCCCCCEEECCCHHHHHHHHHHCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA