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The map label for this gene is nuoF [H]

Identifier: 116329149

GI number: 116329149

Start: 3052063

End: 3053397

Strand: Reverse

Name: nuoF [H]

Synonym: LBL_2573

Alternate gene names: 116329149

Gene position: 3053397-3052063 (Counterclockwise)

Preceding gene: 116329150

Following gene: 116329148

Centisome position: 84.48

GC content: 47.94

Gene sequence:

>1335_bases
ATGGCAGAAATGAAACTTCTTACGAAATACATAGACAACCCTCAGTCCGCAAAACTTGAGTTTTACGAATCGGTACACGG
TTACGAAGGTTTAAAAAAAGCGCTTTCGATGAAACCGGACGATATCATCGCAGAAGTCAAAAAGTCGGGTCTGCGAGGGA
GAGGGGGCGCCGGTTTTCCTACCGGACTCAAGTGGTCCTTTATCCCGAAGGACGTTTCCAAACCGAAATATATCATCTGT
AACGCAGACGAAGGCGAGCCCGGAACATTCAAAGATCGTAAACTGATTGAGAATCTTCCGCACCAAATCATAGAAGGAAT
GATTATCGGAGCGAAAGCCATCGGCTCGAACAAAGGATTCTTTTACATTCGGGGAGAATTTCAGAAAGGCGCTAGGACAA
TGCAGGCCGCGATCGACGAGGCGTATTCCAAAGGGTATCTCGGAAAGAATATTCTAGGCACCGATTTCGACTTCGATCTA
ATTCTCTACGAGGGTGCGGGCGCTTACATCTGTGGAGAAGAAACCGCGCTCATCAATTCTCTCGAAGGCAGGAGAGGCCA
TCCAAGATTGAAGCCTCCCTTCCCCGCCGTCTCCGGTTTGTATCGTTCTCCGACGGTGGTCAATAACGTGGAAACGTTTT
CCGCGATTCCTCATATTTTGGACAAAGGCGCAGATTGGTATTCCAAAATCGGAACCGAAAAATCTCCGGGAACGAGACTT
TTCAGCGTTTCGGGTCACGTAAAAAGACCGGGTGTCTACGAAATCGAATTAGGAACTCCTTTATTAGAACTTGTGAATGA
TCTTTGTGGAGGAATCGTGGATGATAAACCTCTCAAGGCGATCATTCCGGGAGGGTCTTCCGTTCCGATTCTCACGGCGG
AAGAATGTAAAACCGCGAACATGGACTTCGAATCCATGGCGGCTCATAAGACGATGCTCGGTTCGGGCGCGGTCATCGTG
CTTGCGGAAGGCGCGGACATCGTGGAAACCACGTATCGTTTTGCGAGGTTTTATGCTCACGAATCCTGCGGTCAGTGTAC
ACCTTGTAGAGAAGGTACACATTGGGTTCGGGATATTCTTTATAAAATCAGAGAAGGAGAAGGAACGACTCAAGATATTG
ATTTGATTCTTTCCTTATCCAGAAATATGGAAGGTGGAACTACGATTTGTCCGCTTTCTGACGCTTGTGTGGGAGCGGTT
CGTCCTGCGATTCAGAAATTTCGTTCGGAATTTGACGCGAGATTGAAAGAAAAACCGGCGCCCGTACCCGATCATCCTCG
AAGGAGAAAAGAGGATAGGATCGAGTCCGCAATAGGAGGAAGAGCTACTTCATGA

Upstream 100 bases:

>100_bases
CGCTCCGATGGTCCAGATAGGCGACGATTTTTACGAGCAACTAACTCCCGAAAAAGTGGACCGGATTTTAGATAACCTGA
ACTGACAAAAGGAAATCCTT

Downstream 100 bases:

>100_bases
ACTGGAACGAAATTCTATTTTGGCTTTTGAAAAGCGGGCTTTTCTTTTTTATTCTGATTACCGCCTGCGCGTATTATACG
CTCGCAGAAAGAAAGGTGGC

Product: NADH dehydrogenase (ubiquinone), F chain

Products: NA

Alternate protein names: NADH dehydrogenase I chain 1; NDH-1 subunit 1 [H]

Number of amino acids: Translated: 444; Mature: 443

Protein sequence:

>444_residues
MAEMKLLTKYIDNPQSAKLEFYESVHGYEGLKKALSMKPDDIIAEVKKSGLRGRGGAGFPTGLKWSFIPKDVSKPKYIIC
NADEGEPGTFKDRKLIENLPHQIIEGMIIGAKAIGSNKGFFYIRGEFQKGARTMQAAIDEAYSKGYLGKNILGTDFDFDL
ILYEGAGAYICGEETALINSLEGRRGHPRLKPPFPAVSGLYRSPTVVNNVETFSAIPHILDKGADWYSKIGTEKSPGTRL
FSVSGHVKRPGVYEIELGTPLLELVNDLCGGIVDDKPLKAIIPGGSSVPILTAEECKTANMDFESMAAHKTMLGSGAVIV
LAEGADIVETTYRFARFYAHESCGQCTPCREGTHWVRDILYKIREGEGTTQDIDLILSLSRNMEGGTTICPLSDACVGAV
RPAIQKFRSEFDARLKEKPAPVPDHPRRRKEDRIESAIGGRATS

Sequences:

>Translated_444_residues
MAEMKLLTKYIDNPQSAKLEFYESVHGYEGLKKALSMKPDDIIAEVKKSGLRGRGGAGFPTGLKWSFIPKDVSKPKYIIC
NADEGEPGTFKDRKLIENLPHQIIEGMIIGAKAIGSNKGFFYIRGEFQKGARTMQAAIDEAYSKGYLGKNILGTDFDFDL
ILYEGAGAYICGEETALINSLEGRRGHPRLKPPFPAVSGLYRSPTVVNNVETFSAIPHILDKGADWYSKIGTEKSPGTRL
FSVSGHVKRPGVYEIELGTPLLELVNDLCGGIVDDKPLKAIIPGGSSVPILTAEECKTANMDFESMAAHKTMLGSGAVIV
LAEGADIVETTYRFARFYAHESCGQCTPCREGTHWVRDILYKIREGEGTTQDIDLILSLSRNMEGGTTICPLSDACVGAV
RPAIQKFRSEFDARLKEKPAPVPDHPRRRKEDRIESAIGGRATS
>Mature_443_residues
AEMKLLTKYIDNPQSAKLEFYESVHGYEGLKKALSMKPDDIIAEVKKSGLRGRGGAGFPTGLKWSFIPKDVSKPKYIICN
ADEGEPGTFKDRKLIENLPHQIIEGMIIGAKAIGSNKGFFYIRGEFQKGARTMQAAIDEAYSKGYLGKNILGTDFDFDLI
LYEGAGAYICGEETALINSLEGRRGHPRLKPPFPAVSGLYRSPTVVNNVETFSAIPHILDKGADWYSKIGTEKSPGTRLF
SVSGHVKRPGVYEIELGTPLLELVNDLCGGIVDDKPLKAIIPGGSSVPILTAEECKTANMDFESMAAHKTMLGSGAVIVL
AEGADIVETTYRFARFYAHESCGQCTPCREGTHWVRDILYKIREGEGTTQDIDLILSLSRNMEGGTTICPLSDACVGAVR
PAIQKFRSEFDARLKEKPAPVPDHPRRRKEDRIESAIGGRATS

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is menaquinone. Couples the redox reaction to proton translocation (for every

COG id: COG1894

COG function: function code C; NADH:ubiquinone oxidoreductase, NADH-binding (51 kD) subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 51 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI260656005, Length=388, Percent_Identity=48.1958762886598, Blast_Score=386, Evalue=1e-107,
Organism=Homo sapiens, GI20149568, Length=388, Percent_Identity=48.1958762886598, Blast_Score=386, Evalue=1e-107,
Organism=Escherichia coli, GI1788620, Length=414, Percent_Identity=46.6183574879227, Blast_Score=382, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI17531727, Length=380, Percent_Identity=50.2631578947368, Blast_Score=400, Evalue=1e-112,
Organism=Drosophila melanogaster, GI19920794, Length=380, Percent_Identity=48.4210526315789, Blast_Score=387, Evalue=1e-108,
Organism=Drosophila melanogaster, GI45550452, Length=382, Percent_Identity=48.4293193717278, Blast_Score=384, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24653819, Length=384, Percent_Identity=36.9791666666667, Blast_Score=278, Evalue=5e-75,
Organism=Drosophila melanogaster, GI24653817, Length=355, Percent_Identity=34.6478873239437, Blast_Score=243, Evalue=1e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001949
- InterPro:   IPR019575
- InterPro:   IPR011537
- InterPro:   IPR011538
- InterPro:   IPR019554 [H]

Pfam domain/function: PF01512 Complex1_51K; PF10589 NADH_4Fe-4S; PF10531 SLBB [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 48583; Mature: 48452

Theoretical pI: Translated: 7.47; Mature: 7.47

Prosite motif: PS00644 COMPLEX1_51K_1 ; PS00645 COMPLEX1_51K_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEMKLLTKYIDNPQSAKLEFYESVHGYEGLKKALSMKPDDIIAEVKKSGLRGRGGAGFP
CCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCC
TGLKWSFIPKDVSKPKYIICNADEGEPGTFKDRKLIENLPHQIIEGMIIGAKAIGSNKGF
CCCEEEECCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCEEECHHEECCCCCE
FYIRGEFQKGARTMQAAIDEAYSKGYLGKNILGTDFDFDLILYEGAGAYICGEETALINS
EEEECCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCEEEECCHHHHHHH
LEGRRGHPRLKPPFPAVSGLYRSPTVVNNVETFSAIPHILDKGADWYSKIGTEKSPGTRL
CCCCCCCCCCCCCCHHHHCCCCCCCHHCCHHHHHHHHHHHHCCCHHHHHCCCCCCCCCEE
FSVSGHVKRPGVYEIELGTPLLELVNDLCGGIVDDKPLKAIIPGGSSVPILTAEECKTAN
EEECCCCCCCCEEEEECCCHHHHHHHHHHCCCCCCCCCEEEECCCCCCCEEEHHHCCCCC
MDFESMAAHKTMLGSGAVIVLAEGADIVETTYRFARFYAHESCGQCTPCREGTHWVRDIL
CCHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
YKIREGEGTTQDIDLILSLSRNMEGGTTICPLSDACVGAVRPAIQKFRSEFDARLKEKPA
HHHHCCCCCHHHHHHHHHHHCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
PVPDHPRRRKEDRIESAIGGRATS
CCCCCCHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
AEMKLLTKYIDNPQSAKLEFYESVHGYEGLKKALSMKPDDIIAEVKKSGLRGRGGAGFP
CHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCC
TGLKWSFIPKDVSKPKYIICNADEGEPGTFKDRKLIENLPHQIIEGMIIGAKAIGSNKGF
CCCEEEECCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCEEECHHEECCCCCE
FYIRGEFQKGARTMQAAIDEAYSKGYLGKNILGTDFDFDLILYEGAGAYICGEETALINS
EEEECCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCEEEECCHHHHHHH
LEGRRGHPRLKPPFPAVSGLYRSPTVVNNVETFSAIPHILDKGADWYSKIGTEKSPGTRL
CCCCCCCCCCCCCCHHHHCCCCCCCHHCCHHHHHHHHHHHHCCCHHHHHCCCCCCCCCEE
FSVSGHVKRPGVYEIELGTPLLELVNDLCGGIVDDKPLKAIIPGGSSVPILTAEECKTAN
EEECCCCCCCCEEEEECCCHHHHHHHHHHCCCCCCCCCEEEECCCCCCCEEEHHHCCCCC
MDFESMAAHKTMLGSGAVIVLAEGADIVETTYRFARFYAHESCGQCTPCREGTHWVRDIL
CCHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
YKIREGEGTTQDIDLILSLSRNMEGGTTICPLSDACVGAVRPAIQKFRSEFDARLKEKPA
HHHHCCCCCHHHHHHHHHHHCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
PVPDHPRRRKEDRIESAIGGRATS
CCCCCCHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9020134 [H]