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The map label for this gene is nuoE [H]

Identifier: 116329150

GI number: 116329150

Start: 3053413

End: 3053892

Strand: Reverse

Name: nuoE [H]

Synonym: LBL_2574

Alternate gene names: 116329150

Gene position: 3053892-3053413 (Counterclockwise)

Preceding gene: 116329151

Following gene: 116329149

Centisome position: 84.49

GC content: 46.88

Gene sequence:

>480_bases
ATGAGTTACAAGTTTAGTGAAGCCTCCGAAAAAAGATTCCGGAAGATGTTGGAAGTATTTCCGGACAAACGTTCTCTCGT
TCTGCCTTGTTTGTACATTCTCCAAAGGGAAAACGGTTTTGTGGATCAGGACGGAATGGCTTACATCGCCGAACGTCTGG
GAGATCCGATCTCTCTCGCACAAGTCTACGGGGTCGCCACGTTTTACACTCTCTACAACAAAAAACCGGTGGGCAAATAT
CACATTCAGATTTGCGGAACTTCGAGTTGTTATCTGCTGGGAAGCGACGAAATCGAAGAACATATCTGCAAACGTTTGGG
AATTCATATGGGACAGACGACCTCGGACGGTAAGTTTACCTTGGAAGAAGTGGAATGTCTGGGCGCTTGCGGCTACGCTC
CGATGGTCCAGATAGGCGACGATTTTTACGAGCAACTAACTCCCGAAAAAGTGGACCGGATTTTAGATAACCTGAACTGA

Upstream 100 bases:

>100_bases
ATTATCAATCGTTTGCGGAACTCGTGCGCGGAGGACTTCTCGCGGATTCGGTCGCGACCATGAGTTCTTTGAACGTGATT
GCGGGGGAGCTGGACTGCTG

Downstream 100 bases:

>100_bases
CAAAAGGAAATCCTTATGGCAGAAATGAAACTTCTTACGAAATACATAGACAACCCTCAGTCCGCAAAACTTGAGTTTTA
CGAATCGGTACACGGTTACG

Product: NADH dehydrogenase (ubiquinone), E chain

Products: NA

Alternate protein names: NADH dehydrogenase I subunit E; NDH-1 subunit E [H]

Number of amino acids: Translated: 159; Mature: 158

Protein sequence:

>159_residues
MSYKFSEASEKRFRKMLEVFPDKRSLVLPCLYILQRENGFVDQDGMAYIAERLGDPISLAQVYGVATFYTLYNKKPVGKY
HIQICGTSSCYLLGSDEIEEHICKRLGIHMGQTTSDGKFTLEEVECLGACGYAPMVQIGDDFYEQLTPEKVDRILDNLN

Sequences:

>Translated_159_residues
MSYKFSEASEKRFRKMLEVFPDKRSLVLPCLYILQRENGFVDQDGMAYIAERLGDPISLAQVYGVATFYTLYNKKPVGKY
HIQICGTSSCYLLGSDEIEEHICKRLGIHMGQTTSDGKFTLEEVECLGACGYAPMVQIGDDFYEQLTPEKVDRILDNLN
>Mature_158_residues
SYKFSEASEKRFRKMLEVFPDKRSLVLPCLYILQRENGFVDQDGMAYIAERLGDPISLAQVYGVATFYTLYNKKPVGKYH
IQICGTSSCYLLGSDEIEEHICKRLGIHMGQTTSDGKFTLEEVECLGACGYAPMVQIGDDFYEQLTPEKVDRILDNLN

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cyto

COG id: COG1905

COG function: function code C; NADH:ubiquinone oxidoreductase 24 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 24 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI222080062, Length=158, Percent_Identity=50, Blast_Score=154, Evalue=3e-38,
Organism=Escherichia coli, GI1788621, Length=155, Percent_Identity=34.8387096774194, Blast_Score=100, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI17561328, Length=160, Percent_Identity=45.625, Blast_Score=157, Evalue=2e-39,
Organism=Drosophila melanogaster, GI18859877, Length=160, Percent_Identity=46.875, Blast_Score=149, Evalue=9e-37,
Organism=Drosophila melanogaster, GI21355353, Length=161, Percent_Identity=36.0248447204969, Blast_Score=111, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002023
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF01257 Complex1_24kDa [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 18072; Mature: 17941

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS01099 COMPLEX1_24K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
6.9 %Cys+Met (Translated Protein)
3.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYKFSEASEKRFRKMLEVFPDKRSLVLPCLYILQRENGFVDQDGMAYIAERLGDPISLA
CCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCHHHH
QVYGVATFYTLYNKKPVGKYHIQICGTSSCYLLGSDEIEEHICKRLGIHMGQTTSDGKFT
HHHHHHHHHHHHCCCCCCEEEEEEECCCCEEEECCHHHHHHHHHHHCCCCCCCCCCCCEE
LEEVECLGACGYAPMVQIGDDFYEQLTPEKVDRILDNLN
HHHHHHHHCCCCCCHHHHCHHHHHHCCHHHHHHHHHCCC
>Mature Secondary Structure 
SYKFSEASEKRFRKMLEVFPDKRSLVLPCLYILQRENGFVDQDGMAYIAERLGDPISLA
CCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCHHHH
QVYGVATFYTLYNKKPVGKYHIQICGTSSCYLLGSDEIEEHICKRLGIHMGQTTSDGKFT
HHHHHHHHHHHHCCCCCCEEEEEEECCCCEEEECCHHHHHHHHHHHCCCCCCCCCCCCEE
LEEVECLGACGYAPMVQIGDDFYEQLTPEKVDRILDNLN
HHHHHHHHCCCCCCHHHHCHHHHHHCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]