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The map label for this gene is spoT [H]

Identifier: 116328856

GI number: 116328856

Start: 2638840

End: 2640864

Strand: Reverse

Name: spoT [H]

Synonym: LBL_2240

Alternate gene names: 116328856

Gene position: 2640864-2638840 (Counterclockwise)

Preceding gene: 116328857

Following gene: 116328855

Centisome position: 73.06

GC content: 44.1

Gene sequence:

>2025_bases
ATGGGATTTGTAAAAACTCCTGCAACTAAAGATATGCTACTAGAAGGAGTTCGTGAAACGCTTGGAGAGAGCGCCTATGA
ATCTGTTCAGAAGGCGTATGATGTCTCTGAACGAGCACATCAGGGACAGTTTCGTCTTTCGGGAGAACCTTATATCGTCC
ACCCTCTTCAAGTGGGATTTATTCTTTATGAGCTTGGTTTGGACGAAAAAGTCATCTGCGCCGGCCTTCTTCATGATGTC
ATTGAGGACACTCAGTATTCTCGAGACGATATGATCCGAGATTTCGGAGAAGACATCACGGATCTAGTGGAAGGTGTCAC
TAAAATTTCTAAGATCAAAAGTCAATCCAAGGAGACCGAGGCGGCGGAGAATATCCGAAAGATTATCGTAGCGACAATCA
AGGATATCCGAGTCATTCTCATCAAACTTGCGGATAAAACTCATAATATGAGAACTCTTTCGTTCCAACTTCCGGAAAAA
CAAAAAAGAATCGCTCAAGAAACCCTTTCTTTATACGCTCCGATCGCCGGGCGATTGGGAATTTATAAAATCAAATCCGA
ATTGGAAGATCTTGCGTTTCAGGTTTTAAATCCCGAAGAATATCAAGAAGTAAAGAAGAATATCAATTCCAAAAAATCGG
AAAGGGAAGGATTTATCGAAACTCTGAAGATCATCCTTCTTCAAAGACTTTCCGAGATTCAGATCGAAGCGGATGTGGAC
GGAAGAGCCAAACACTTCTATTCCATCTATCGCAAGATGAAACTTAAAGAGAAAACCTTTAACGAAATTTTCGATCTTAG
AGCGATCCGTATCATCACGAACGAAGTAAAAGATTGTTATGGAGTATTAGGAATCGTGCATACTCTTTGGAATCCTGTTC
CGGGCCGTTTTAAGGATTATATCGCCACTCCGAAGACGAACATGTATCAATCTCTTCACACGACGGTGATAGGGCCCGAC
GGTAAACCTCTCGAAGTTCAGATTCGTACCCGCGATATGAACGATATCGCCGAATACGGAATCGCCGCACACTGGATGTA
TAAAGAGGGAAAACCTTCCCTATCCGAAAAGAACGTAAAGGTAAGATGGCTAGAGCTATTAAGTTCTTGGCAAGACTCGG
CGTTAGATCCGAAGGAATTTGTGGAAGAACTCAAATACGATCTTCACGAAGACGAAGTATTCGTATTCACTCCGAAAGGT
GAAATCCTTCAGCTTCCTAAGGGTGCGACGATCCTTGATTTCGCGTTTCGGATTCATACCGATGTCGGTTTAAAAGCGAA
AGGTGGAAGGATCAACGGGAGGATGCTTCCTCTCCGAACCGAACTTCGTTCCGGGGATCAGATCGAAATCATCACCGACA
AAAGAACAAAACCTTCCCCAATTTGGTTACGTATCGTTAGAACTCCTTCCGCAAGACAAAAGTTGAGGAGTTATTTTCGG
AAGCTAAAAGAGGAAAATAAAAAGGATCTTCAGCAAGAAGCCGAGTTCGCCGCAGAGATTACGCTTAACGTGGAAGTTTT
GGAAGAGCTTAAAAAGAAGCCTCTTGCAAAACCCGCGAAACAAATCGACTTGGGCGCGGGAAAAGTGATTGTCGCGGGTC
TTCGCGGAATTCTGGCGAGACTTTCCGGCTGTTGTTCCCCTCTGCCGGGGGACGGGATCATCGGATTTGTCACTCGAGGG
AGGGGAGTCAGTATCCACAAAAAAGGATGTGCCACCGCCAAACAGCAGGAACAGGAAGAATCCATGCGCATCATCACGGT
CGAATGGGATTACGGTCAAAGCGAATCGATTCCCGTGTTAATTGAAGTGAAATCGAAAGACCGGCAAGGCATCTTTATGG
AAATCGTAAAATCCATTTCCAATACGCAGACCAACATCAGAGAATCCAAAGCAAGCACCGATCAAAGAGGAAACTTGGTC
GCTAACTTCGAGGTGGATGTGGAACATTTGGATCAACTCAAGGAGATCCTAAGTAATCTCAAACAAATCCCGGACGTATA
TCAAGCGCATAGAATTAAAAATTAA

Upstream 100 bases:

>100_bases
CGCTCTCGGAATTTTCGGGAGCCGGTGTTAGTATGGGTTATCTAACGCATACGACTCGTTTTCTAGACCTCGGTTTGGAT
ATAGAGCAACACTAACAACT

Downstream 100 bases:

>100_bases
ACGAGGTGATTTTTTGAAAAGATATTCTATTTTTTGCATGTTTTTTGTGCTCTCTGTTTTCACTTTTCTTGCATGCGGAG
ATAAAGAAGAATTCGAAACG

Product: GTP pyrophosphokinase

Products: NA

Alternate protein names: (p)ppGpp synthase; ATP:GTP 3'-pyrophosphotransferase; ppGpp synthase I [H]

Number of amino acids: Translated: 674; Mature: 673

Protein sequence:

>674_residues
MGFVKTPATKDMLLEGVRETLGESAYESVQKAYDVSERAHQGQFRLSGEPYIVHPLQVGFILYELGLDEKVICAGLLHDV
IEDTQYSRDDMIRDFGEDITDLVEGVTKISKIKSQSKETEAAENIRKIIVATIKDIRVILIKLADKTHNMRTLSFQLPEK
QKRIAQETLSLYAPIAGRLGIYKIKSELEDLAFQVLNPEEYQEVKKNINSKKSEREGFIETLKIILLQRLSEIQIEADVD
GRAKHFYSIYRKMKLKEKTFNEIFDLRAIRIITNEVKDCYGVLGIVHTLWNPVPGRFKDYIATPKTNMYQSLHTTVIGPD
GKPLEVQIRTRDMNDIAEYGIAAHWMYKEGKPSLSEKNVKVRWLELLSSWQDSALDPKEFVEELKYDLHEDEVFVFTPKG
EILQLPKGATILDFAFRIHTDVGLKAKGGRINGRMLPLRTELRSGDQIEIITDKRTKPSPIWLRIVRTPSARQKLRSYFR
KLKEENKKDLQQEAEFAAEITLNVEVLEELKKKPLAKPAKQIDLGAGKVIVAGLRGILARLSGCCSPLPGDGIIGFVTRG
RGVSIHKKGCATAKQQEQEESMRIITVEWDYGQSESIPVLIEVKSKDRQGIFMEIVKSISNTQTNIRESKASTDQRGNLV
ANFEVDVEHLDQLKEILSNLKQIPDVYQAHRIKN

Sequences:

>Translated_674_residues
MGFVKTPATKDMLLEGVRETLGESAYESVQKAYDVSERAHQGQFRLSGEPYIVHPLQVGFILYELGLDEKVICAGLLHDV
IEDTQYSRDDMIRDFGEDITDLVEGVTKISKIKSQSKETEAAENIRKIIVATIKDIRVILIKLADKTHNMRTLSFQLPEK
QKRIAQETLSLYAPIAGRLGIYKIKSELEDLAFQVLNPEEYQEVKKNINSKKSEREGFIETLKIILLQRLSEIQIEADVD
GRAKHFYSIYRKMKLKEKTFNEIFDLRAIRIITNEVKDCYGVLGIVHTLWNPVPGRFKDYIATPKTNMYQSLHTTVIGPD
GKPLEVQIRTRDMNDIAEYGIAAHWMYKEGKPSLSEKNVKVRWLELLSSWQDSALDPKEFVEELKYDLHEDEVFVFTPKG
EILQLPKGATILDFAFRIHTDVGLKAKGGRINGRMLPLRTELRSGDQIEIITDKRTKPSPIWLRIVRTPSARQKLRSYFR
KLKEENKKDLQQEAEFAAEITLNVEVLEELKKKPLAKPAKQIDLGAGKVIVAGLRGILARLSGCCSPLPGDGIIGFVTRG
RGVSIHKKGCATAKQQEQEESMRIITVEWDYGQSESIPVLIEVKSKDRQGIFMEIVKSISNTQTNIRESKASTDQRGNLV
ANFEVDVEHLDQLKEILSNLKQIPDVYQAHRIKN
>Mature_673_residues
GFVKTPATKDMLLEGVRETLGESAYESVQKAYDVSERAHQGQFRLSGEPYIVHPLQVGFILYELGLDEKVICAGLLHDVI
EDTQYSRDDMIRDFGEDITDLVEGVTKISKIKSQSKETEAAENIRKIIVATIKDIRVILIKLADKTHNMRTLSFQLPEKQ
KRIAQETLSLYAPIAGRLGIYKIKSELEDLAFQVLNPEEYQEVKKNINSKKSEREGFIETLKIILLQRLSEIQIEADVDG
RAKHFYSIYRKMKLKEKTFNEIFDLRAIRIITNEVKDCYGVLGIVHTLWNPVPGRFKDYIATPKTNMYQSLHTTVIGPDG
KPLEVQIRTRDMNDIAEYGIAAHWMYKEGKPSLSEKNVKVRWLELLSSWQDSALDPKEFVEELKYDLHEDEVFVFTPKGE
ILQLPKGATILDFAFRIHTDVGLKAKGGRINGRMLPLRTELRSGDQIEIITDKRTKPSPIWLRIVRTPSARQKLRSYFRK
LKEENKKDLQQEAEFAAEITLNVEVLEELKKKPLAKPAKQIDLGAGKVIVAGLRGILARLSGCCSPLPGDGIIGFVTRGR
GVSIHKKGCATAKQQEQEESMRIITVEWDYGQSESIPVLIEVKSKDRQGIFMEIVKSISNTQTNIRESKASTDQRGNLVA
NFEVDVEHLDQLKEILSNLKQIPDVYQAHRIKN

Specific function: In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the formation of pppGpp which i

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HD domain [H]

Homologues:

Organism=Escherichia coli, GI1790082, Length=697, Percent_Identity=40.4591104734577, Blast_Score=516, Evalue=1e-147,
Organism=Escherichia coli, GI1789147, Length=678, Percent_Identity=34.2182890855457, Blast_Score=421, Evalue=1e-119,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR012675
- InterPro:   IPR003607
- InterPro:   IPR007685
- InterPro:   IPR004811
- InterPro:   IPR004095
- InterPro:   IPR012676 [H]

Pfam domain/function: PF01842 ACT; PF04607 RelA_SpoT; PF02824 TGS [H]

EC number: =2.7.6.5 [H]

Molecular weight: Translated: 76743; Mature: 76611

Theoretical pI: Translated: 8.57; Mature: 8.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGFVKTPATKDMLLEGVRETLGESAYESVQKAYDVSERAHQGQFRLSGEPYIVHPLQVGF
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCEEEEHHHHHH
ILYELGLDEKVICAGLLHDVIEDTQYSRDDMIRDFGEDITDLVEGVTKISKIKSQSKETE
HHHCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAENIRKIIVATIKDIRVILIKLADKTHNMRTLSFQLPEKQKRIAQETLSLYAPIAGRLG
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHHHHHHCHHHCCH
IYKIKSELEDLAFQVLNPEEYQEVKKNINSKKSEREGFIETLKIILLQRLSEIQIEADVD
HHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCEEEECCC
GRAKHFYSIYRKMKLKEKTFNEIFDLRAIRIITNEVKDCYGVLGIVHTLWNPVPGRFKDY
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
IATPKTNMYQSLHTTVIGPDGKPLEVQIRTRDMNDIAEYGIAAHWMYKEGKPSLSEKNVK
HCCCCHHHHHHHHHEEECCCCCEEEEEEEECCHHHHHHHCCEEEEEECCCCCCCCCCCCH
VRWLELLSSWQDSALDPKEFVEELKYDLHEDEVFVFTPKGEILQLPKGATILDFAFRIHT
HHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCEEECCCCCCHHHHHHHHCC
DVGLKAKGGRINGRMLPLRTELRSGDQIEIITDKRTKPSPIWLRIVRTPSARQKLRSYFR
CCCCEECCCCCCCEEEEEHHHHCCCCEEEEEECCCCCCCCEEEEEEECCHHHHHHHHHHH
KLKEENKKDLQQEAEFAAEITLNVEVLEELKKKPLAKPAKQIDLGAGKVIVAGLRGILAR
HHHHHHHHHHHHHHHHHHHEEEEHHHHHHHHHCCCCCCHHHHCCCCCHHHHHHHHHHHHH
LSGCCSPLPGDGIIGFVTRGRGVSIHKKGCATAKQQEQEESMRIITVEWDYGQSESIPVL
HHHCCCCCCCCCEEEEEECCCCCEEECCCCCCHHHHHHHCCEEEEEEEECCCCCCCCEEE
IEVKSKDRQGIFMEIVKSISNTQTNIRESKASTDQRGNLVANFEVDVEHLDQLKEILSNL
EEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECHHHHHHHHHHHHHH
KQIPDVYQAHRIKN
HHCHHHHHHHCCCC
>Mature Secondary Structure 
GFVKTPATKDMLLEGVRETLGESAYESVQKAYDVSERAHQGQFRLSGEPYIVHPLQVGF
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCEEEEHHHHHH
ILYELGLDEKVICAGLLHDVIEDTQYSRDDMIRDFGEDITDLVEGVTKISKIKSQSKETE
HHHCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAENIRKIIVATIKDIRVILIKLADKTHNMRTLSFQLPEKQKRIAQETLSLYAPIAGRLG
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHHHHHHCHHHCCH
IYKIKSELEDLAFQVLNPEEYQEVKKNINSKKSEREGFIETLKIILLQRLSEIQIEADVD
HHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCEEEECCC
GRAKHFYSIYRKMKLKEKTFNEIFDLRAIRIITNEVKDCYGVLGIVHTLWNPVPGRFKDY
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
IATPKTNMYQSLHTTVIGPDGKPLEVQIRTRDMNDIAEYGIAAHWMYKEGKPSLSEKNVK
HCCCCHHHHHHHHHEEECCCCCEEEEEEEECCHHHHHHHCCEEEEEECCCCCCCCCCCCH
VRWLELLSSWQDSALDPKEFVEELKYDLHEDEVFVFTPKGEILQLPKGATILDFAFRIHT
HHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCEEECCCCCCHHHHHHHHCC
DVGLKAKGGRINGRMLPLRTELRSGDQIEIITDKRTKPSPIWLRIVRTPSARQKLRSYFR
CCCCEECCCCCCCEEEEEHHHHCCCCEEEEEECCCCCCCCEEEEEEECCHHHHHHHHHHH
KLKEENKKDLQQEAEFAAEITLNVEVLEELKKKPLAKPAKQIDLGAGKVIVAGLRGILAR
HHHHHHHHHHHHHHHHHHHEEEEHHHHHHHHHCCCCCCHHHHCCCCCHHHHHHHHHHHHH
LSGCCSPLPGDGIIGFVTRGRGVSIHKKGCATAKQQEQEESMRIITVEWDYGQSESIPVL
HHHCCCCCCCCCEEEEEECCCCCEEECCCCCCHHHHHHHCCEEEEEEEECCCCCCCCEEE
IEVKSKDRQGIFMEIVKSISNTQTNIRESKASTDQRGNLVANFEVDVEHLDQLKEILSNL
EEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECHHHHHHHHHHHHHH
KQIPDVYQAHRIKN
HHCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9383190; 9384377 [H]