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The map label for this gene is sbp [H]

Identifier: 116328768

GI number: 116328768

Start: 2514143

End: 2515153

Strand: Reverse

Name: sbp [H]

Synonym: LBL_2143

Alternate gene names: 116328768

Gene position: 2515153-2514143 (Counterclockwise)

Preceding gene: 116328769

Following gene: 116328767

Centisome position: 69.59

GC content: 43.42

Gene sequence:

>1011_bases
ATGAAATTCAAATCTTTAAGAATTCCTTTCACTGTATTGTTCTTTGTTTCATTTTCATTGTTTTCAGAAACCAAACTTTT
GAACGTTTCTTACGATCCGACTCGGGAATTGTATTCTCAGATCAATAAACTCTTCATCGCACATTGGAAGAAACAAACCG
GAGAAGACTTGGTCATTCACCAATCTCACGGAGGTTCCGGTAAACAAGCAAGGGCCGTTATTGACGGATTGGAAGCGGAT
GTCGTAACCTTGGCTCTTGCGCACGATATCGAAGCGATTGCGGAAAAAACGAAACTTCTTTCCACCGATTGGGAAAATTT
GCTTCCTCATCAGAGTTCTCCTTATACCTCTACGATCGTATTTTTGGTCAGAAAAGGAAATCCAAAAGGAATCAAGGACT
GGGATGATCTTGTGAAACCGGGGGTCGGAGTGATTGCGCCGAATCCTAAAACCGGAGGTGGGGCGCGTTGGAATTATCTT
GCGGCCTGGGGTTACGCGAAAGAAAAATACGGGTCGGAAGAAAAAGCGAAAACCTTTGTAAAAAGTCTTTATGCGAATGT
TCTCGTTTTGGACTCGGGTGCAAGAGGTTCCTTAACTACGTTTGTACAGAGAGGGCTCGGAGACGTTCTCATCTCTTGGG
AAAACGAGGCTCATTTGGCGCTTCAAGAAACCGCAAAAGATTCGAGTTCCTCCGTTGAAATCGTATTTCCATCCGTGAGT
ATTTTAGCCGAACCTTCCGTAGCGGTCGTCACTAAAAATGCGGAAAAACACAATACTTTCAAAGTCGCCGAGAGTTATTT
GAAATTTCTATACACTCCGGAAGGTCAGGAAACGATCGCGAATCATTTCTATCGACCTACGGATAAGAAGATCGCGTCCA
AATATAAGAGTTTATTCAAAAATTTGAAACTCTTCACGATTCGAGACGTTGCCGGTTCCTGGAAGAAGGCCCAGGAAATG
CATTTTGCGGACGGTGCGATTTTCGATCAGATTACGAGCAAGACTAAATAA

Upstream 100 bases:

>100_bases
TCTTTACTTTTAGAGGAGATTTAAGAATATTGGATCAAATTATCTAATAAATTAGATTTTTTTTCGCTTTGTTGTATGAT
TTGTCCGAGGAGAGAACGAA

Downstream 100 bases:

>100_bases
AAAGTTTTCGGGTTCAAGCGGAGAAAATTGTGTCTACTTTGAATTTTAGGACCAAACCCTACGGAAAAACCGCTTTCGGT
ATTACGTTAGGCGTTACGAT

Product: substrate binding protein of an ABC transporter complex

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: Sulfate starvation-induced protein 2; SSI2 [H]

Number of amino acids: Translated: 336; Mature: 336

Protein sequence:

>336_residues
MKFKSLRIPFTVLFFVSFSLFSETKLLNVSYDPTRELYSQINKLFIAHWKKQTGEDLVIHQSHGGSGKQARAVIDGLEAD
VVTLALAHDIEAIAEKTKLLSTDWENLLPHQSSPYTSTIVFLVRKGNPKGIKDWDDLVKPGVGVIAPNPKTGGGARWNYL
AAWGYAKEKYGSEEKAKTFVKSLYANVLVLDSGARGSLTTFVQRGLGDVLISWENEAHLALQETAKDSSSSVEIVFPSVS
ILAEPSVAVVTKNAEKHNTFKVAESYLKFLYTPEGQETIANHFYRPTDKKIASKYKSLFKNLKLFTIRDVAGSWKKAQEM
HFADGAIFDQITSKTK

Sequences:

>Translated_336_residues
MKFKSLRIPFTVLFFVSFSLFSETKLLNVSYDPTRELYSQINKLFIAHWKKQTGEDLVIHQSHGGSGKQARAVIDGLEAD
VVTLALAHDIEAIAEKTKLLSTDWENLLPHQSSPYTSTIVFLVRKGNPKGIKDWDDLVKPGVGVIAPNPKTGGGARWNYL
AAWGYAKEKYGSEEKAKTFVKSLYANVLVLDSGARGSLTTFVQRGLGDVLISWENEAHLALQETAKDSSSSVEIVFPSVS
ILAEPSVAVVTKNAEKHNTFKVAESYLKFLYTPEGQETIANHFYRPTDKKIASKYKSLFKNLKLFTIRDVAGSWKKAQEM
HFADGAIFDQITSKTK
>Mature_336_residues
MKFKSLRIPFTVLFFVSFSLFSETKLLNVSYDPTRELYSQINKLFIAHWKKQTGEDLVIHQSHGGSGKQARAVIDGLEAD
VVTLALAHDIEAIAEKTKLLSTDWENLLPHQSSPYTSTIVFLVRKGNPKGIKDWDDLVKPGVGVIAPNPKTGGGARWNYL
AAWGYAKEKYGSEEKAKTFVKSLYANVLVLDSGARGSLTTFVQRGLGDVLISWENEAHLALQETAKDSSSSVEIVFPSVS
ILAEPSVAVVTKNAEKHNTFKVAESYLKFLYTPEGQETIANHFYRPTDKKIASKYKSLFKNLKLFTIRDVAGSWKKAQEM
HFADGAIFDQITSKTK

Specific function: This protein specifically binds sulfate and is involved in its transmembrane transport [H]

COG id: COG1613

COG function: function code P; ABC-type sulfate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic sulfate-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1790351, Length=328, Percent_Identity=61.280487804878, Blast_Score=408, Evalue=1e-115,
Organism=Escherichia coli, GI1788765, Length=310, Percent_Identity=45.4838709677419, Blast_Score=273, Evalue=1e-74,

Paralogues:

None

Copy number: 1240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR000957
- InterPro:   IPR005669 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 37411; Mature: 37411

Theoretical pI: Translated: 9.74; Mature: 9.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
0.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFKSLRIPFTVLFFVSFSLFSETKLLNVSYDPTRELYSQINKLFIAHWKKQTGEDLVIH
CCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEEE
QSHGGSGKQARAVIDGLEADVVTLALAHDIEAIAEKTKLLSTDWENLLPHQSSPYTSTIV
CCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCEEEE
FLVRKGNPKGIKDWDDLVKPGVGVIAPNPKTGGGARWNYLAAWGYAKEKYGSEEKAKTFV
EEEECCCCCCCCCHHHHHCCCCCEECCCCCCCCCCCCCEEEECCCHHHHCCCHHHHHHHH
KSLYANVLVLDSGARGSLTTFVQRGLGDVLISWENEAHLALQETAKDSSSSVEIVFPSVS
HHHHHCEEEEECCCCCHHHHHHHHCCCCEEEEECCCCEEEEHHHCCCCCCCEEEEECCHH
ILAEPSVAVVTKNAEKHNTFKVAESYLKFLYTPEGQETIANHFYRPTDKKIASKYKSLFK
HHCCCCEEEEECCCCHHHHHHHHHHHHHHEECCCCHHHHHHHCCCCCHHHHHHHHHHHHH
NLKLFTIRDVAGSWKKAQEMHFADGAIFDQITSKTK
CCEEEEEECHHCCHHHHHHHHHCCCHHHHHHHCCCC
>Mature Secondary Structure
MKFKSLRIPFTVLFFVSFSLFSETKLLNVSYDPTRELYSQINKLFIAHWKKQTGEDLVIH
CCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEEE
QSHGGSGKQARAVIDGLEADVVTLALAHDIEAIAEKTKLLSTDWENLLPHQSSPYTSTIV
CCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCEEEE
FLVRKGNPKGIKDWDDLVKPGVGVIAPNPKTGGGARWNYLAAWGYAKEKYGSEEKAKTFV
EEEECCCCCCCCCHHHHHCCCCCEECCCCCCCCCCCCCEEEECCCHHHHCCCHHHHHHHH
KSLYANVLVLDSGARGSLTTFVQRGLGDVLISWENEAHLALQETAKDSSSSVEIVFPSVS
HHHHHCEEEEECCCCCHHHHHHHHCCCCEEEEECCCCEEEEHHHCCCCCCCEEEEECCHH
ILAEPSVAVVTKNAEKHNTFKVAESYLKFLYTPEGQETIANHFYRPTDKKIASKYKSLFK
HHCCCCEEEEECCCCHHHHHHHHHHHHHHEECCCCHHHHHHHCCCCCHHHHHHHHHHHHH
NLKLFTIRDVAGSWKKAQEMHFADGAIFDQITSKTK
CCEEEEEECHHCCHHHHHHHHHCCCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3158524; 2002055; 8346018; 9278503; 9298646; 8774726 [H]