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The map label for this gene is cysU [H]

Identifier: 116328767

GI number: 116328767

Start: 2513277

End: 2514113

Strand: Reverse

Name: cysU [H]

Synonym: LBL_2142

Alternate gene names: 116328767

Gene position: 2514113-2513277 (Counterclockwise)

Preceding gene: 116328768

Following gene: 116328766

Centisome position: 69.56

GC content: 44.92

Gene sequence:

>837_bases
GTGTCTACTTTGAATTTTAGGACCAAACCCTACGGAAAAACCGCTTTCGGTATTACGTTAGGCGTTACGATCACATACCT
AAGTTTGATCGTCATTATTCCGTTAGGCGCTCTTTTTCTGAAAAGTTCCGGGATCGGTTGGGAAGGTTTTTGGAAACTTC
TTACTCATGAGAGAATTTTATCCGCCCTTTACTTAAGTTTCGGAGCCGGAGCCGTTGCGGCGATCATCAATCTGTTCGTC
GGATTTTTGTTCGCATGGGTTTTGGTCCGTTATCGTTTTCCCGGAAAATCCTTGCTCGACTCTCTTGTGGATCTTCCTTT
TACGTTACCGACCGCCGTTGCCGGAATCGCGCTCACTGCAATTTATACTCCCAACGGAGTCGTCGGGAAACTTTTCGAAC
CTTACGGAATCAAGATTGCATATACTCCGGCCGGAATCGTAATCGCCCTCGTATTCATCGGTTTTCCTTTTGTCGTAAGA
ACGGTTCAACCCGTTCTGGAAGAATTTCCGAAAGAACTCGAAGAATCTGCGTATTGTTTAGGCGCCGATCGTTGGCAGAT
TTTTAGAAAAGTGATTTTTCCGGAACTCTTTCCTTCGCTCGTTACGGGAGCTGCGATGGCATTTGCGAGAGGAATCGGAG
AATACGGTTCCGTCGTTTTCATTTCGGGAAACCTTCCGGGTAAAACGGAAATTCTGCCTTTGCTCATCGTCACGAAGTTA
GAACAATACGAATACGAAGAAGCAACCGGAATAGCGTTTATCATGCTTGTAGTCTCTTTTAGTATATTATTGTGTATTAA
TATTATTCAGACGGTTGCCGCAAGGAGAAAGGGATGA

Upstream 100 bases:

>100_bases
GGAAGAAGGCCCAGGAAATGCATTTTGCGGACGGTGCGATTTTCGATCAGATTACGAGCAAGACTAAATAAAAAGTTTTC
GGGTTCAAGCGGAGAAAATT

Downstream 100 bases:

>100_bases
GACAAGAATCTTTACTCGTTCGAAGTATTCTTATCGGAGCGGTCTTTTTACTGACCGGATTGATTTTGATCCTTCCTATC
TATACGGTTTTTCACGAGGC

Product: ABC transporter permease

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 278; Mature: 277

Protein sequence:

>278_residues
MSTLNFRTKPYGKTAFGITLGVTITYLSLIVIIPLGALFLKSSGIGWEGFWKLLTHERILSALYLSFGAGAVAAIINLFV
GFLFAWVLVRYRFPGKSLLDSLVDLPFTLPTAVAGIALTAIYTPNGVVGKLFEPYGIKIAYTPAGIVIALVFIGFPFVVR
TVQPVLEEFPKELEESAYCLGADRWQIFRKVIFPELFPSLVTGAAMAFARGIGEYGSVVFISGNLPGKTEILPLLIVTKL
EQYEYEEATGIAFIMLVVSFSILLCINIIQTVAARRKG

Sequences:

>Translated_278_residues
MSTLNFRTKPYGKTAFGITLGVTITYLSLIVIIPLGALFLKSSGIGWEGFWKLLTHERILSALYLSFGAGAVAAIINLFV
GFLFAWVLVRYRFPGKSLLDSLVDLPFTLPTAVAGIALTAIYTPNGVVGKLFEPYGIKIAYTPAGIVIALVFIGFPFVVR
TVQPVLEEFPKELEESAYCLGADRWQIFRKVIFPELFPSLVTGAAMAFARGIGEYGSVVFISGNLPGKTEILPLLIVTKL
EQYEYEEATGIAFIMLVVSFSILLCINIIQTVAARRKG
>Mature_277_residues
STLNFRTKPYGKTAFGITLGVTITYLSLIVIIPLGALFLKSSGIGWEGFWKLLTHERILSALYLSFGAGAVAAIINLFVG
FLFAWVLVRYRFPGKSLLDSLVDLPFTLPTAVAGIALTAIYTPNGVVGKLFEPYGIKIAYTPAGIVIALVFIGFPFVVRT
VQPVLEEFPKELEESAYCLGADRWQIFRKVIFPELFPSLVTGAAMAFARGIGEYGSVVFISGNLPGKTEILPLLIVTKLE
QYEYEEATGIAFIMLVVSFSILLCINIIQTVAARRKG

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG0555

COG function: function code O; ABC-type sulfate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788764, Length=261, Percent_Identity=44.8275862068966, Blast_Score=225, Evalue=2e-60,
Organism=Escherichia coli, GI87082099, Length=240, Percent_Identity=32.5, Blast_Score=135, Evalue=2e-33,
Organism=Escherichia coli, GI1786980, Length=220, Percent_Identity=33.6363636363636, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1787368, Length=239, Percent_Identity=28.4518828451883, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1786254, Length=224, Percent_Identity=25.8928571428571, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1787369, Length=171, Percent_Identity=26.9005847953216, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR011865
- InterPro:   IPR005667 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 30376; Mature: 30244

Theoretical pI: Translated: 9.20; Mature: 9.20

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTLNFRTKPYGKTAFGITLGVTITYLSLIVIIPLGALFLKSSGIGWEGFWKLLTHERIL
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
SALYLSFGAGAVAAIINLFVGFLFAWVLVRYRFPGKSLLDSLVDLPFTLPTAVAGIALTA
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHE
IYTPNGVVGKLFEPYGIKIAYTPAGIVIALVFIGFPFVVRTVQPVLEEFPKELEESAYCL
EECCCCCHHHCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GADRWQIFRKVIFPELFPSLVTGAAMAFARGIGEYGSVVFISGNLPGKTEILPLLIVTKL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHH
EQYEYEEATGIAFIMLVVSFSILLCINIIQTVAARRKG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
STLNFRTKPYGKTAFGITLGVTITYLSLIVIIPLGALFLKSSGIGWEGFWKLLTHERIL
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
SALYLSFGAGAVAAIINLFVGFLFAWVLVRYRFPGKSLLDSLVDLPFTLPTAVAGIALTA
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHE
IYTPNGVVGKLFEPYGIKIAYTPAGIVIALVFIGFPFVVRTVQPVLEEFPKELEESAYCL
EECCCCCHHHCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GADRWQIFRKVIFPELFPSLVTGAAMAFARGIGEYGSVVFISGNLPGKTEILPLLIVTKL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHH
EQYEYEEATGIAFIMLVVSFSILLCINIIQTVAARRKG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2188958; 9205837; 9278503; 2188959 [H]