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The map label for this gene is bioD

Identifier: 116328114

GI number: 116328114

Start: 1645084

End: 1645749

Strand: Direct

Name: bioD

Synonym: LBL_1412

Alternate gene names: 116328114

Gene position: 1645084-1645749 (Clockwise)

Preceding gene: 116328113

Following gene: 116328115

Centisome position: 45.51

GC content: 41.59

Gene sequence:

>666_bases
ATGGCAGTATTTATCGGAGCCACTGGAACGGACATAGGTAAAACTCTTTTTAGTTCCCTCATCTTGGGGAAATACGGAAA
GTCCTTAGGACTCAAGTATTTTAAGCCCGTTCAAACCGGAAATGATAGCGATCGGGTCACTTTGATGAACCTTACGGGCC
TTCACGAAAGTTATTTTCTCAAAAACTATTATTCCTTGTCTTTTGCCGGATCTCCTCACTATGCTTCCGAATTGGAAGGG
GTTGAGATCGATTCGGATGAACTTTCCAGACATCTTTACAGCATTCGCGACGAAAAAATCATCGTGGAAGGAGCCGGTGG
TCTACTTGTTCCGCTGACTCGAAAAATTCTTACTTTAGAATTGGTCCGACAATCCGAGATTCCTCTCATTTTAGTGGCTC
CTGTTTCGTTGGGTGCGATCAATCAAACCCTTCTTGCAATCGAAGCTGTCCAAAATCGAAAGATCGATTTAAAGGGAATT
TATTTCATAGGTATCCCCGATAAAACCACGGAAGATAATATTCGAACGATCACGGAATGGAGTGGAGTGACTTTGCTTGG
AAATTTTTTTCTGAACTCAAAAGAAAAAATGAGTCGGGAACGTTTTCAAATCGAATGTCTTTCCCGATTCGATCAGTGCG
AAGTAATCAAGAAAATGTTCGTATGA

Upstream 100 bases:

>100_bases
GTCCTCCAACCGTGAGCGTTCCCAGACTTCGAGTCAGCATTCATTCCGATACAACGGAATCAATATTAGAAAAATTGATT
TCTCTTTTGCCGGAGTTTTG

Downstream 100 bases:

>100_bases
TTTGGTATCCGTTTACGCTTCAGTTCGAATCCGATCCTCCCTTAAAGATAAAGAGGGCAAAAGGAGAATTTTTGTATGAC
GAAAACGGAAACTCTTATAT

Product: dethiobiotin synthetase

Products: NA

Alternate protein names: DTB synthetase; DTBS; Dethiobiotin synthase

Number of amino acids: Translated: 221; Mature: 220

Protein sequence:

>221_residues
MAVFIGATGTDIGKTLFSSLILGKYGKSLGLKYFKPVQTGNDSDRVTLMNLTGLHESYFLKNYYSLSFAGSPHYASELEG
VEIDSDELSRHLYSIRDEKIIVEGAGGLLVPLTRKILTLELVRQSEIPLILVAPVSLGAINQTLLAIEAVQNRKIDLKGI
YFIGIPDKTTEDNIRTITEWSGVTLLGNFFLNSKEKMSRERFQIECLSRFDQCEVIKKMFV

Sequences:

>Translated_221_residues
MAVFIGATGTDIGKTLFSSLILGKYGKSLGLKYFKPVQTGNDSDRVTLMNLTGLHESYFLKNYYSLSFAGSPHYASELEG
VEIDSDELSRHLYSIRDEKIIVEGAGGLLVPLTRKILTLELVRQSEIPLILVAPVSLGAINQTLLAIEAVQNRKIDLKGI
YFIGIPDKTTEDNIRTITEWSGVTLLGNFFLNSKEKMSRERFQIECLSRFDQCEVIKKMFV
>Mature_220_residues
AVFIGATGTDIGKTLFSSLILGKYGKSLGLKYFKPVQTGNDSDRVTLMNLTGLHESYFLKNYYSLSFAGSPHYASELEGV
EIDSDELSRHLYSIRDEKIIVEGAGGLLVPLTRKILTLELVRQSEIPLILVAPVSLGAINQTLLAIEAVQNRKIDLKGIY
FIGIPDKTTEDNIRTITEWSGVTLLGNFFLNSKEKMSRERFQIECLSRFDQCEVIKKMFV

Specific function: Bioconversion of pimelate into dethiobiotin. [C]

COG id: COG0132

COG function: function code H; Dethiobiotin synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dethiobiotin synthetase family

Homologues:

Organism=Escherichia coli, GI1786995, Length=198, Percent_Identity=30.8080808080808, Blast_Score=85, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOD_LEPBJ (Q04RS7)

Other databases:

- EMBL:   CP000350
- RefSeq:   YP_801151.1
- ProteinModelPortal:   Q04RS7
- SMR:   Q04RS7
- STRING:   Q04RS7
- GeneID:   4410112
- GenomeReviews:   CP000350_GR
- KEGG:   lbj:LBJ_1872
- eggNOG:   COG0132
- HOGENOM:   HBG650065
- OMA:   WKPIQSG
- PhylomeDB:   Q04RS7
- ProtClustDB:   CLSK574238
- BioCyc:   LBOR355277:LBJ_1872-MONOMER
- HAMAP:   MF_00336
- InterPro:   IPR004472
- PIRSF:   PIRSF006755
- TIGRFAMs:   TIGR00347

Pfam domain/function: NA

EC number: =6.3.3.3

Molecular weight: Translated: 24720; Mature: 24589

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVFIGATGTDIGKTLFSSLILGKYGKSLGLKYFKPVQTGNDSDRVTLMNLTGLHESYFL
CEEEEECCCCHHHHHHHHHHHHHHCCHHCCCCEECCCCCCCCCCCEEEEEECCCCHHHHH
KNYYSLSFAGSPHYASELEGVEIDSDELSRHLYSIRDEKIIVEGAGGLLVPLTRKILTLE
HHEEEEEECCCCCHHHHCCCCEECHHHHHHHHHHHCCCEEEEECCCCEEHHHHHHHHHHH
LVRQSEIPLILVAPVSLGAINQTLLAIEAVQNRKIDLKGIYFIGIPDKTTEDNIRTITEW
HHHCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCEEEEEEEEEECCCCCCCCCCEEEECC
SGVTLLGNFFLNSKEKMSRERFQIECLSRFDQCEVIKKMFV
CCEEEEEHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCC
>Mature Secondary Structure 
AVFIGATGTDIGKTLFSSLILGKYGKSLGLKYFKPVQTGNDSDRVTLMNLTGLHESYFL
EEEEECCCCHHHHHHHHHHHHHHCCHHCCCCEECCCCCCCCCCCEEEEEECCCCHHHHH
KNYYSLSFAGSPHYASELEGVEIDSDELSRHLYSIRDEKIIVEGAGGLLVPLTRKILTLE
HHEEEEEECCCCCHHHHCCCCEECHHHHHHHHHHHCCCEEEEECCCCEEHHHHHHHHHHH
LVRQSEIPLILVAPVSLGAINQTLLAIEAVQNRKIDLKGIYFIGIPDKTTEDNIRTITEW
HHHCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCEEEEEEEEEECCCCCCCCCCEEEECC
SGVTLLGNFFLNSKEKMSRERFQIECLSRFDQCEVIKKMFV
CCEEEEEHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA