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The map label for this gene is ppiB-2 [H]

Identifier: 116328058

GI number: 116328058

Start: 1586400

End: 1587275

Strand: Direct

Name: ppiB-2 [H]

Synonym: LBL_1353

Alternate gene names: 116328058

Gene position: 1586400-1587275 (Clockwise)

Preceding gene: 116328057

Following gene: 116328059

Centisome position: 43.89

GC content: 42.47

Gene sequence:

>876_bases
ATGAGAATTATTTCTGGAATTTTAACGTTTGTTTGTTTTTTAGCCTGTAATCGAAATCCGTTTGCACAGACCAGATACCA
ACCGGAAGTTTATACACCGTCTGCCGTCGTAGTTCCAAGACAAGATATGTCCGTGGTTCCATTACCGGAAAAGCCCGCGA
TTTATGCGATTTTTTTGACTACTCAAGGAACTATGATTGTTGAGTTATTCGACCAAGACGCCCCTAAAACGGTTCAGAAC
TTTATCGACCTGGCACAAGGCGAGAAGGAATTTCTGACTAGAAACGGACAAAAGGTGAAAAAACCTTTTTACGACGGTTT
GACCTTTCATAGAGTGATCGAAAATTTTATGATCCAGGGCGGTTGTCCGAATGGGGACGGAACCGGCGGTCCCGGATATC
GTTTTGATGACGAGATCAATGGAAAAGCCCTCGGTCTAGATCAGATGAAAGCTGGGGAAACTCCGTACTATCAATACCAA
CTTCAAAAGGCCGTGGTTAGCGAACTTCAGATTAAAAACAATGAAGAGGCGGAAGCAAAAAGAGAATTGATCGAAAAGGC
ATTTGAAGACGCCAAGAAGTTATCGGTTCTTGAAATTCTTTTTAGAATCGGATATAAATACAATGAAATTCTAAAGTCCC
ATAAGGCGCTTAAAGGTTCTCTTGCAATGGCGAACGCGGGACCGAATACAAACGGTTCCCAATTCTTTATCAATCAAGTG
GATACTCCGCATTTGGACGGTCTTCATACTGTTTTCGGTCAATTGATATCCGGCGCGGATGTTGTGGACAAAATCGTAAA
GATGGGAAACTCCAAAACTTCGATCAAAAAAGTTCTCATATTAGATAAGAGAACGGCGGCTACGGCGGTGCAATGA

Upstream 100 bases:

>100_bases
CGGACAAAAAAATCTCTTACGGAGGAACTTCCAAAAAAAGACAGGAAGAACAATTAAAAATTGCGCTGGAATCTTTGAAG
CAAACTGAAACGTTGTTTTT

Downstream 100 bases:

>100_bases
CATTTACACCGGATAAAATCCAGGCAAAGAATTATCTTACAGTGATTCAAGAACTGGCGAATTATTCTTCCACCAGCGAC
ACGAGTCGTATTTTAGAGCG

Product: peptidylprolyl isomerase

Products: NA

Alternate protein names: PPIase; Rotamase [H]

Number of amino acids: Translated: 291; Mature: 291

Protein sequence:

>291_residues
MRIISGILTFVCFLACNRNPFAQTRYQPEVYTPSAVVVPRQDMSVVPLPEKPAIYAIFLTTQGTMIVELFDQDAPKTVQN
FIDLAQGEKEFLTRNGQKVKKPFYDGLTFHRVIENFMIQGGCPNGDGTGGPGYRFDDEINGKALGLDQMKAGETPYYQYQ
LQKAVVSELQIKNNEEAEAKRELIEKAFEDAKKLSVLEILFRIGYKYNEILKSHKALKGSLAMANAGPNTNGSQFFINQV
DTPHLDGLHTVFGQLISGADVVDKIVKMGNSKTSIKKVLILDKRTAATAVQ

Sequences:

>Translated_291_residues
MRIISGILTFVCFLACNRNPFAQTRYQPEVYTPSAVVVPRQDMSVVPLPEKPAIYAIFLTTQGTMIVELFDQDAPKTVQN
FIDLAQGEKEFLTRNGQKVKKPFYDGLTFHRVIENFMIQGGCPNGDGTGGPGYRFDDEINGKALGLDQMKAGETPYYQYQ
LQKAVVSELQIKNNEEAEAKRELIEKAFEDAKKLSVLEILFRIGYKYNEILKSHKALKGSLAMANAGPNTNGSQFFINQV
DTPHLDGLHTVFGQLISGADVVDKIVKMGNSKTSIKKVLILDKRTAATAVQ
>Mature_291_residues
MRIISGILTFVCFLACNRNPFAQTRYQPEVYTPSAVVVPRQDMSVVPLPEKPAIYAIFLTTQGTMIVELFDQDAPKTVQN
FIDLAQGEKEFLTRNGQKVKKPFYDGLTFHRVIENFMIQGGCPNGDGTGGPGYRFDDEINGKALGLDQMKAGETPYYQYQ
LQKAVVSELQIKNNEEAEAKRELIEKAFEDAKKLSVLEILFRIGYKYNEILKSHKALKGSLAMANAGPNTNGSQFFINQV
DTPHLDGLHTVFGQLISGADVVDKIVKMGNSKTSIKKVLILDKRTAATAVQ

Specific function: PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides [H]

COG id: COG0652

COG function: function code O; Peptidyl-prolyl cis-trans isomerase (rotamase) - cyclophilin family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase cyclophilin-type domain [H]

Homologues:

Organism=Homo sapiens, GI19557636, Length=209, Percent_Identity=28.7081339712919, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI5031987, Length=65, Percent_Identity=56.9230769230769, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI10863927, Length=66, Percent_Identity=54.5454545454545, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI4826932, Length=45, Percent_Identity=68.8888888888889, Blast_Score=66, Evalue=3e-11,
Organism=Homo sapiens, GI7706339, Length=102, Percent_Identity=36.2745098039216, Blast_Score=65, Evalue=5e-11,
Organism=Homo sapiens, GI14277126, Length=69, Percent_Identity=49.2753623188406, Blast_Score=65, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI17565860, Length=65, Percent_Identity=64.6153846153846, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17566266, Length=76, Percent_Identity=48.6842105263158, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17551282, Length=62, Percent_Identity=53.2258064516129, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17551284, Length=62, Percent_Identity=53.2258064516129, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17539496, Length=99, Percent_Identity=41.4141414141414, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17559074, Length=76, Percent_Identity=47.3684210526316, Blast_Score=67, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI17506307, Length=55, Percent_Identity=61.8181818181818, Blast_Score=67, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI17506311, Length=90, Percent_Identity=40, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17552780, Length=80, Percent_Identity=47.5, Blast_Score=64, Evalue=7e-11,
Organism=Saccharomyces cerevisiae, GI6320359, Length=76, Percent_Identity=47.3684210526316, Blast_Score=78, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6323562, Length=76, Percent_Identity=46.0526315789474, Blast_Score=69, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6320510, Length=66, Percent_Identity=51.5151515151515, Blast_Score=67, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6323246, Length=42, Percent_Identity=69.0476190476191, Blast_Score=65, Evalue=1e-11,
Organism=Drosophila melanogaster, GI19922376, Length=65, Percent_Identity=47.6923076923077, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI17647301, Length=63, Percent_Identity=53.968253968254, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI45549139, Length=65, Percent_Identity=52.3076923076923, Blast_Score=66, Evalue=3e-11,

Paralogues:

None

Copy number: 780 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015891
- InterPro:   IPR020892
- InterPro:   IPR002130 [H]

Pfam domain/function: PF00160 Pro_isomerase [H]

EC number: =5.2.1.8 [H]

Molecular weight: Translated: 32332; Mature: 32332

Theoretical pI: Translated: 8.41; Mature: 8.41

Prosite motif: PS00170 CSA_PPIASE_1 ; PS50072 CSA_PPIASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIISGILTFVCFLACNRNPFAQTRYQPEVYTPSAVVVPRQDMSVVPLPEKPAIYAIFLT
CHHHHHHHHHHHHHHCCCCCCHHCCCCCCEECCCEEEEECCCCEEECCCCCCCEEEEEEE
TQGTMIVELFDQDAPKTVQNFIDLAQGEKEFLTRNGQKVKKPFYDGLTFHRVIENFMIQG
CCCEEEEEEECCCCHHHHHHHHHHHCCHHHHHHCCCCHHHHHHHCCHHHHHHHHHHHCCC
GCPNGDGTGGPGYRFDDEINGKALGLDQMKAGETPYYQYQLQKAVVSELQIKNNEEAEAK
CCCCCCCCCCCCCCCCCCCCCCEECHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHH
RELIEKAFEDAKKLSVLEILFRIGYKYNEILKSHKALKGSLAMANAGPNTNGSQFFINQV
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCEEEECCCCCCCCCEEEEECC
DTPHLDGLHTVFGQLISGADVVDKIVKMGNSKTSIKKVLILDKRTAATAVQ
CCCCCCHHHHHHHHHHCCHHHHHHHHHCCCCHHHHEEEEEEECCHHHHCCC
>Mature Secondary Structure
MRIISGILTFVCFLACNRNPFAQTRYQPEVYTPSAVVVPRQDMSVVPLPEKPAIYAIFLT
CHHHHHHHHHHHHHHCCCCCCHHCCCCCCEECCCEEEEECCCCEEECCCCCCCEEEEEEE
TQGTMIVELFDQDAPKTVQNFIDLAQGEKEFLTRNGQKVKKPFYDGLTFHRVIENFMIQG
CCCEEEEEEECCCCHHHHHHHHHHHCCHHHHHHCCCCHHHHHHHCCHHHHHHHHHHHCCC
GCPNGDGTGGPGYRFDDEINGKALGLDQMKAGETPYYQYQLQKAVVSELQIKNNEEAEAK
CCCCCCCCCCCCCCCCCCCCCCEECHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHH
RELIEKAFEDAKKLSVLEILFRIGYKYNEILKSHKALKGSLAMANAGPNTNGSQFFINQV
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCEEEECCCCCCCCCEEEEECC
DTPHLDGLHTVFGQLISGADVVDKIVKMGNSKTSIKKVLILDKRTAATAVQ
CCCCCCHHHHHHHHHHCCHHHHHHHHHCCCCHHHHEEEEEEECCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9317025; 9665876 [H]