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The map label for this gene is purA [H]

Identifier: 116327200

GI number: 116327200

Start: 450948

End: 452225

Strand: Reverse

Name: purA [H]

Synonym: LBL_0387

Alternate gene names: 116327200

Gene position: 452225-450948 (Counterclockwise)

Preceding gene: 116327201

Following gene: 116327198

Centisome position: 12.51

GC content: 43.27

Gene sequence:

>1278_bases
ATGCCCGCATCGTTAGTAGTAGGGACCCAATGGGGTGATGAAGGAAAAGCCAAAGTTATCGACTTTCTTTCCAAGGACAC
AGACATCATTGTTCGTTACCAAGGAGGTGCGAACGCGGGACATACGGTCGTCGTCCATGGAAAAAAATACGTATTTCATT
TAGTCCCTTCCGGGGTGATCTACGATCAAACTATCTGTGTAATCGGAAACGGAGTTGTCCTCGACCCTCTTTTTTTCATC
GAAGAATGTGATCGTCTTCAAAAGGAAGGATTTCCGGTCTACGACAAACTTTTGTTAAGCGATGCGTGTCATCTTCTTTT
TCCGTATCATTCTCAGATCGATTCTGCAAGGGAAACTACGGTCAGCCAAGAACACAAAATCGGAACCACAAAAAAAGGAA
TCGGAATCTGTTACGCGGATAAAATGATGAGAACCGGACTGCGAGTAGGAGATCTTTTAGACAATTCCTACGAGTCCCGT
TTGAAACATTTGGTCGACGAAAAAAATCGCGAACTCGACAAACTCTACGGAATGCCCCCCGTTTCTTATAAAGATATCAA
CGACGGTTTAAAATTCTTTCTTTCCAAAGTGAGAAAAAGCATTATAAATACTGCATATTATCTCGATACGGAACTTAAGA
AAGGGAAACGGGTCCTTTTGGAAGGCGCCCAAGGAACTGGTTTGGACGTCGACTTCGGAACCTATCCTTACGTCACAAGT
TCCAATCCCACAACCGGAGGAGCATTGATCGGAACCGGAATTTCGTTTCAACATTTGAAACACGTTATTGGAATTACAAA
AGCGTACACTACAAGAGTCGGAGAGGGACCTTTCCCTACGGAACTTTTAGGAGAAGAGGGAGAGGCTCTCCGTCAAAAAG
GCGGGGAATTTGGAGCGACCACGGGACGTCCGAGACGATGTGGTTGGTTCGACGCAGAGATGCTAAAACATTCAGTTCGT
ATTAATGGGATCACTTCGATTGCTTTAACAAAGATTGATATTCTTTCCGATTACGATCGGATTCCGGTTGCGGTCGGCTA
TAAACTGAACGGAAAAACTCTAGATTGTTTTCCATCGCAAGGTTTGGAAAAACTAGAAATCGTTTATGAAGAATTTCCGG
GATGGAAAACGGATATTTCCGGCATCTCCGAATTTCAAAAACTCCCTGAGAAATGTAAAAATTATATTTCCGCTCTGGAG
AAATGGATCGGAGTGAAAATCAATTTGGTCTCAACCGGTCCTGATAGAAAGGATACGATTCAAGGGGATTCTTTTTAA

Upstream 100 bases:

>100_bases
GCTCTTATCGTGAATATTTTAGAAGCTTCCCTTAAAAACCCCCTTTCCGATTCCAAATCATGAAACGCAATCGTACCAAA
TATCTATTTGAGGAAAAACT

Downstream 100 bases:

>100_bases
GAATCTAAGGTTTTTTTCAACGTTTTTGATTGACCCACCACACTCGATAAATATCGTGTATTTCGGTAACGCTTCGAGTC
GTTAGCTCAGCTGGTAGAGC

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase [H]

Number of amino acids: Translated: 425; Mature: 424

Protein sequence:

>425_residues
MPASLVVGTQWGDEGKAKVIDFLSKDTDIIVRYQGGANAGHTVVVHGKKYVFHLVPSGVIYDQTICVIGNGVVLDPLFFI
EECDRLQKEGFPVYDKLLLSDACHLLFPYHSQIDSARETTVSQEHKIGTTKKGIGICYADKMMRTGLRVGDLLDNSYESR
LKHLVDEKNRELDKLYGMPPVSYKDINDGLKFFLSKVRKSIINTAYYLDTELKKGKRVLLEGAQGTGLDVDFGTYPYVTS
SNPTTGGALIGTGISFQHLKHVIGITKAYTTRVGEGPFPTELLGEEGEALRQKGGEFGATTGRPRRCGWFDAEMLKHSVR
INGITSIALTKIDILSDYDRIPVAVGYKLNGKTLDCFPSQGLEKLEIVYEEFPGWKTDISGISEFQKLPEKCKNYISALE
KWIGVKINLVSTGPDRKDTIQGDSF

Sequences:

>Translated_425_residues
MPASLVVGTQWGDEGKAKVIDFLSKDTDIIVRYQGGANAGHTVVVHGKKYVFHLVPSGVIYDQTICVIGNGVVLDPLFFI
EECDRLQKEGFPVYDKLLLSDACHLLFPYHSQIDSARETTVSQEHKIGTTKKGIGICYADKMMRTGLRVGDLLDNSYESR
LKHLVDEKNRELDKLYGMPPVSYKDINDGLKFFLSKVRKSIINTAYYLDTELKKGKRVLLEGAQGTGLDVDFGTYPYVTS
SNPTTGGALIGTGISFQHLKHVIGITKAYTTRVGEGPFPTELLGEEGEALRQKGGEFGATTGRPRRCGWFDAEMLKHSVR
INGITSIALTKIDILSDYDRIPVAVGYKLNGKTLDCFPSQGLEKLEIVYEEFPGWKTDISGISEFQKLPEKCKNYISALE
KWIGVKINLVSTGPDRKDTIQGDSF
>Mature_424_residues
PASLVVGTQWGDEGKAKVIDFLSKDTDIIVRYQGGANAGHTVVVHGKKYVFHLVPSGVIYDQTICVIGNGVVLDPLFFIE
ECDRLQKEGFPVYDKLLLSDACHLLFPYHSQIDSARETTVSQEHKIGTTKKGIGICYADKMMRTGLRVGDLLDNSYESRL
KHLVDEKNRELDKLYGMPPVSYKDINDGLKFFLSKVRKSIINTAYYLDTELKKGKRVLLEGAQGTGLDVDFGTYPYVTSS
NPTTGGALIGTGISFQHLKHVIGITKAYTTRVGEGPFPTELLGEEGEALRQKGGEFGATTGRPRRCGWFDAEMLKHSVRI
NGITSIALTKIDILSDYDRIPVAVGYKLNGKTLDCFPSQGLEKLEIVYEEFPGWKTDISGISEFQKLPEKCKNYISALEK
WIGVKINLVSTGPDRKDTIQGDSF

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP [H]

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family [H]

Homologues:

Organism=Homo sapiens, GI22748717, Length=429, Percent_Identity=45.6876456876457, Blast_Score=358, Evalue=5e-99,
Organism=Homo sapiens, GI34577063, Length=428, Percent_Identity=42.5233644859813, Blast_Score=331, Evalue=8e-91,
Organism=Homo sapiens, GI40316944, Length=399, Percent_Identity=44.3609022556391, Blast_Score=316, Evalue=3e-86,
Organism=Escherichia coli, GI1790620, Length=420, Percent_Identity=43.5714285714286, Blast_Score=370, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI25146553, Length=430, Percent_Identity=43.0232558139535, Blast_Score=347, Evalue=9e-96,
Organism=Caenorhabditis elegans, GI25146555, Length=430, Percent_Identity=43.0232558139535, Blast_Score=346, Evalue=1e-95,
Organism=Saccharomyces cerevisiae, GI6324109, Length=435, Percent_Identity=41.6091954022989, Blast_Score=343, Evalue=2e-95,
Organism=Drosophila melanogaster, GI21356033, Length=426, Percent_Identity=43.6619718309859, Blast_Score=339, Evalue=2e-93,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018220
- InterPro:   IPR001114 [H]

Pfam domain/function: PF00709 Adenylsucc_synt [H]

EC number: =6.3.4.4 [H]

Molecular weight: Translated: 47093; Mature: 46962

Theoretical pI: Translated: 7.27; Mature: 7.27

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPASLVVGTQWGDEGKAKVIDFLSKDTDIIVRYQGGANAGHTVVVHGKKYVFHLVPSGVI
CCCCEEEECCCCCCCCHHHHHHHCCCCEEEEEECCCCCCCCEEEEECCEEEEEECCCCEE
YDQTICVIGNGVVLDPLFFIEECDRLQKEGFPVYDKLLLSDACHLLFPYHSQIDSARETT
ECCEEEEECCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHEECCHHHHHHHHHHH
VSQEHKIGTTKKGIGICYADKMMRTGLRVGDLLDNSYESRLKHLVDEKNRELDKLYGMPP
CCHHHCCCCCCCCCEEEEHHHHHHCCCHHHHHHCCHHHHHHHHHHHHHCCHHHHHHCCCC
VSYKDINDGLKFFLSKVRKSIINTAYYLDTELKKGKRVLLEGAQGTGLDVDFGTYPYVTS
CCHHHHHHHHHHHHHHHHHHHHHHHHEEECHHHCCCEEEEECCCCCCCEECCCCCCEEEC
SNPTTGGALIGTGISFQHLKHVIGITKAYTTRVGEGPFPTELLGEEGEALRQKGGEFGAT
CCCCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCHHHHHCCCCCCCC
TGRPRRCGWFDAEMLKHSVRINGITSIALTKIDILSDYDRIPVAVGYKLNGKTLDCFPSQ
CCCCCCCCCCHHHHHHHHHEECCEEEEHHHHHHHHCCCCCCCEEEEEEECCCEEECCCCC
GLEKLEIVYEEFPGWKTDISGISEFQKLPEKCKNYISALEKWIGVKINLVSTGPDRKDTI
CHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCC
QGDSF
CCCCC
>Mature Secondary Structure 
PASLVVGTQWGDEGKAKVIDFLSKDTDIIVRYQGGANAGHTVVVHGKKYVFHLVPSGVI
CCCEEEECCCCCCCCHHHHHHHCCCCEEEEEECCCCCCCCEEEEECCEEEEEECCCCEE
YDQTICVIGNGVVLDPLFFIEECDRLQKEGFPVYDKLLLSDACHLLFPYHSQIDSARETT
ECCEEEEECCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHEECCHHHHHHHHHHH
VSQEHKIGTTKKGIGICYADKMMRTGLRVGDLLDNSYESRLKHLVDEKNRELDKLYGMPP
CCHHHCCCCCCCCCEEEEHHHHHHCCCHHHHHHCCHHHHHHHHHHHHHCCHHHHHHCCCC
VSYKDINDGLKFFLSKVRKSIINTAYYLDTELKKGKRVLLEGAQGTGLDVDFGTYPYVTS
CCHHHHHHHHHHHHHHHHHHHHHHHHEEECHHHCCCEEEEECCCCCCCEECCCCCCEEEC
SNPTTGGALIGTGISFQHLKHVIGITKAYTTRVGEGPFPTELLGEEGEALRQKGGEFGAT
CCCCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCHHHHHCCCCCCCC
TGRPRRCGWFDAEMLKHSVRINGITSIALTKIDILSDYDRIPVAVGYKLNGKTLDCFPSQ
CCCCCCCCCCHHHHHHHHHEECCEEEEHHHHHHHHCCCCCCCEEEEEEECCCEEECCCCC
GLEKLEIVYEEFPGWKTDISGISEFQKLPEKCKNYISALEKWIGVKINLVSTGPDRKDTI
CHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCC
QGDSF
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA