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The map label for this gene is hisZ [H]

Identifier: 116327201

GI number: 116327201

Start: 452263

End: 453297

Strand: Reverse

Name: hisZ [H]

Synonym: LBL_0388

Alternate gene names: 116327201

Gene position: 453297-452263 (Counterclockwise)

Preceding gene: 116327203

Following gene: 116327200

Centisome position: 12.54

GC content: 37.97

Gene sequence:

>1035_bases
ATGAATCAAAATCTTCCAGAACCTAGTCAGAAAAAGTGGATCCCAGATGGTTTTCACTTTCTCGGTCCCGAAGACAGCAA
GGATAGAAGAATTATATTAGAAACCGTATCCGGGATTCTTAAAAAAAAAGGCTATTCGGAAGTATTCTTACCTGCATTTG
ATTATAGTTCAACGTTTCTACAGACGATTTCCACTCCAAATTCTTCGTCTTTGTTTCGAATCCGAGATCTTTCCGGAAAT
GAGATTTCACCTAGTATCGATCTGACCGTTCAAGCGGTTAAGGGGATGGCCGGTTTTTCTCATCAAAGGGAAAATCAAAA
TATCTTTTATATAGGAAGAATTTTCAGAGAAAGCGCAAAAGGGAGCGTATCTCGAAAAGAAGTTCTTCAAATCGGAGCCG
AGTCGATTGGAGTTTCCGGAAAAGAAAACACATTTAAAATTTTGGAAGAATTAAACGAAATTATCAGCATTCTTCCTATC
GAAGACAAGCTAACCCTCGTTTTAGGAAACGTGAATCTGTTTCACTCCATCGTTCGGGAATTTAAATTGAACCCGAGTGA
AATCGAAATCCTTTCCACGCTGCTCTATCAGAAAAATGTAAATGAAATCGAAAGAATTTTCGGAGAAAAAAAGAACAATT
CCATTCTGATTCGTTTGTTAAGCGCACTTGTTTTAAATTTCGATTTGGATTCATTGAAAAATTCCCTCAAGATCGCTTCT
CCTTCAAAGGGCCTTCAAAAAAATTTGAATTCCGTTCTTGAGGACACTTTCTGGATTTTTCAATCTTGGGAATCCAAAAA
GAGAAAAATCGATCTTTGTATCGACTTTTCTCTTTTGAGAGATCTGAACTATTATACCGGTTTTGTTTTTCAAGGTTACC
TGCAAGGTTCTCCCAACCCGGTTTTAACCGGAGGAGCTTACGATCATCTATACGAAATGTTTTCGGGAGTCCAAAGAGAC
GCGAGTGGTTATGCTCTTATCGTGAATATTTTAGAAGCTTCCCTTAAAAACCCCCTTTCCGATTCCAAATCATGA

Upstream 100 bases:

>100_bases
CATTCGTTTACATTGGAATTGTCATTCTATTTTTTTAAAAAGGTTTGAAATCTGACGTCTCAGTTTTGAACTGTATTTTG
AAACTGAAGTATTCCAAAAC

Downstream 100 bases:

>100_bases
AACGCAATCGTACCAAATATCTATTTGAGGAAAAACTATGCCCGCATCGTTAGTAGTAGGGACCCAATGGGGTGATGAAG
GAAAAGCCAAAGTTATCGAC

Product: ATP phosphoribosyltransferase regulatory subunit

Products: AMP; diphosphate; L-histidyl-tRNA(His)

Alternate protein names: NA

Number of amino acids: Translated: 344; Mature: 344

Protein sequence:

>344_residues
MNQNLPEPSQKKWIPDGFHFLGPEDSKDRRIILETVSGILKKKGYSEVFLPAFDYSSTFLQTISTPNSSSLFRIRDLSGN
EISPSIDLTVQAVKGMAGFSHQRENQNIFYIGRIFRESAKGSVSRKEVLQIGAESIGVSGKENTFKILEELNEIISILPI
EDKLTLVLGNVNLFHSIVREFKLNPSEIEILSTLLYQKNVNEIERIFGEKKNNSILIRLLSALVLNFDLDSLKNSLKIAS
PSKGLQKNLNSVLEDTFWIFQSWESKKRKIDLCIDFSLLRDLNYYTGFVFQGYLQGSPNPVLTGGAYDHLYEMFSGVQRD
ASGYALIVNILEASLKNPLSDSKS

Sequences:

>Translated_344_residues
MNQNLPEPSQKKWIPDGFHFLGPEDSKDRRIILETVSGILKKKGYSEVFLPAFDYSSTFLQTISTPNSSSLFRIRDLSGN
EISPSIDLTVQAVKGMAGFSHQRENQNIFYIGRIFRESAKGSVSRKEVLQIGAESIGVSGKENTFKILEELNEIISILPI
EDKLTLVLGNVNLFHSIVREFKLNPSEIEILSTLLYQKNVNEIERIFGEKKNNSILIRLLSALVLNFDLDSLKNSLKIAS
PSKGLQKNLNSVLEDTFWIFQSWESKKRKIDLCIDFSLLRDLNYYTGFVFQGYLQGSPNPVLTGGAYDHLYEMFSGVQRD
ASGYALIVNILEASLKNPLSDSKS
>Mature_344_residues
MNQNLPEPSQKKWIPDGFHFLGPEDSKDRRIILETVSGILKKKGYSEVFLPAFDYSSTFLQTISTPNSSSLFRIRDLSGN
EISPSIDLTVQAVKGMAGFSHQRENQNIFYIGRIFRESAKGSVSRKEVLQIGAESIGVSGKENTFKILEELNEIISILPI
EDKLTLVLGNVNLFHSIVREFKLNPSEIEILSTLLYQKNVNEIERIFGEKKNNSILIRLLSALVLNFDLDSLKNSLKIAS
PSKGLQKNLNSVLEDTFWIFQSWESKKRKIDLCIDFSLLRDLNYYTGFVFQGYLQGSPNPVLTGGAYDHLYEMFSGVQRD
ASGYALIVNILEASLKNPLSDSKS

Specific function: Required for the first step of histidine biosynthesis. May allow the feedback regulation of ATP phosphoribosyltransferase activity by histidine [H]

COG id: COG3705

COG function: function code E; ATP phosphoribosyltransferase involved in histidine biosynthesis

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. HisZ subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004516 [H]

Pfam domain/function: NA

EC number: 6.1.1.21

Molecular weight: Translated: 38773; Mature: 38773

Theoretical pI: Translated: 6.97; Mature: 6.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQNLPEPSQKKWIPDGFHFLGPEDSKDRRIILETVSGILKKKGYSEVFLPAFDYSSTFL
CCCCCCCCHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCHHHHH
QTISTPNSSSLFRIRDLSGNEISPSIDLTVQAVKGMAGFSHQRENQNIFYIGRIFRESAK
HHHCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEHHHHHHHHC
GSVSRKEVLQIGAESIGVSGKENTFKILEELNEIISILPIEDKLTLVLGNVNLFHSIVRE
CCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHH
FKLNPSEIEILSTLLYQKNVNEIERIFGEKKNNSILIRLLSALVLNFDLDSLKNSLKIAS
HCCCHHHHHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHEECCC
PSKGLQKNLNSVLEDTFWIFQSWESKKRKIDLCIDFSLLRDLNYYTGFVFQGYLQGSPNP
CCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEHHHHHHHHHHHHHHEEEEECCCCCC
VLTGGAYDHLYEMFSGVQRDASGYALIVNILEASLKNPLSDSKS
EEECCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MNQNLPEPSQKKWIPDGFHFLGPEDSKDRRIILETVSGILKKKGYSEVFLPAFDYSSTFL
CCCCCCCCHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCHHHHH
QTISTPNSSSLFRIRDLSGNEISPSIDLTVQAVKGMAGFSHQRENQNIFYIGRIFRESAK
HHHCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEHHHHHHHHC
GSVSRKEVLQIGAESIGVSGKENTFKILEELNEIISILPIEDKLTLVLGNVNLFHSIVRE
CCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHH
FKLNPSEIEILSTLLYQKNVNEIERIFGEKKNNSILIRLLSALVLNFDLDSLKNSLKIAS
HCCCHHHHHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHEECCC
PSKGLQKNLNSVLEDTFWIFQSWESKKRKIDLCIDFSLLRDLNYYTGFVFQGYLQGSPNP
CCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEHHHHHHHHHHHHHHEEEEECCCCCC
VLTGGAYDHLYEMFSGVQRDASGYALIVNILEASLKNPLSDSKS
EEECCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Mg2+ [C]

Kcat value (1/min): 8520 [C]

Specific activity: 121.8

Km value (mM): 0.06 {His}} 0.046 {His}} 0.03 {His}} 0.02 {His}} 0.017 {His}} 0.011 {His}} 0.008 {His}} 0.0014 {tRNAHis}} 0.00056 {tRNAHis}} 0.0016 {U73tRNAHisGUG}} 0.00031 {U73tRNAHisGUG}} 0.89 {ATP}} 0.74 {ATP}} 0.655 {ATP}} 0.5

Substrates: ATP; L-histidine; tRNA(His)

Specific reaction: ATP + L-histidine + tRNA(His) = AMP + diphosphate + L-histidyl-tRNA(His)

General reaction: Aminoacylation; Esterification [C]

Inhibitor: 1, 2, 4-Triazole-3-alanine; D-Histidine; Diphosphate; L-Histidinol; Mg2+; N-Acetyl histidine; NEM; p-Chloromercuribenzoate [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA