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The map label for this gene is puuB [H]

Identifier: 115353247

GI number: 115353247

Start: 3533072

End: 3534460

Strand: Direct

Name: puuB [H]

Synonym: Bamb_3196

Alternate gene names: 115353247

Gene position: 3533072-3534460 (Clockwise)

Preceding gene: 115353246

Following gene: 115353250

Centisome position: 99.34

GC content: 69.91

Gene sequence:

>1389_bases
ATGCGACCCTTCTGGATCGAACAAGCACTGTTCAACGACGGCGATCTCGCCCCCGCGCTGCAGGGCGCGACGCAGGCCGA
CGTCTGTATCGTCGGCGGCGGCTTCACCGGGCTCTGGACCGCGATCCAGGCGAAGCAGCAGAACCCGGCGCTCGACATCG
TGATACTCGAGGCCGACCTGTGCGGCGCCGGCGCGAGCGGACGCAATGGCGGGTGCCTGCTCACGTGGTCCGCGAAATTC
CTGACGCTGCGCCGGCTGTTCGGCGAAGCGGAAGCGATCCGGCTCGTGAAGGCGTCGGAGGCGGCCGTCCAGCACATCGC
CGACTTCTGCCGCGCGCATCGCATCGATGCCGAACTGCGGCTCGACGGCACGCTGTACACGGCGACGTCGCGCGCGCAGA
TCGGCACGCTCGCGCCTGTGCTCGACGCGCTCGCCGCGTGCGGCATCCACAGCTACGAACCGCTGCCGGCCGCCGACGTC
GCGCGCCGCTCGGGTTCCGTCCGCAATCTCGACGGCGTGTACTCACTGATCGCCGCGACCGTGCATCCGGGCAAGCTCGC
GCGCGGGCTGCGGCGCGTCGCGCTCGATATGGGGATCCGGATCCACGAGCGCACGCCGCTCGTCGACTTCACGCCGGGGC
AGCCGGCCGTCGTGCGCACGCCGTCCGGCAGCGTGCGCGCGGGCAAGCTGGTGTTCGCGATCAACGCGTGGATGGCGAGC
CGCTTCCCGCAGTTCGAACGCACGATCGCCGTCGTGTCGAGCGACATGGTCATCACCGAAAAATGCCCGGAATTGCTCGA
GCAGACCGGGCTCGTGGACGGCGTATCGGTGCTCGATTCGCGGATCTTCGTGTACTACTACCGCACGACGGCCGATGGTC
GGCTGATGCTCGGCAAGGGCGGCAATACGTTCTCCTGGCGCAGCCGCATCGCGCCGGTGTTCGACCGGCGCTCGCCGTAC
GAGGCGCAGCTCACGCGGAGCCTGCGCGAATTCTTCCCGTCGCTCGCGGGCGTGCCGATCACCGCGAGCTGGAACGGTCC
GTCGGACCGCTCGGTGACGGGCTTCCCGTTCTTCGGCCGCCTCGACGACGCGCCGAACGTGTTCTACGGCTTCGGCTATT
CGGGCAACGGCGTCGGGCCGACCTACATGGGCGGGCAGATCCTGTCGTCGCTGGTGCTCGGCCTCGACAACGCATGGACG
CGCAGCCCGATCGTGCGCGGCCCGCTCGGCCATTTCCCGCCGGAGCCGATCCGCTATGTCGGCGCGCACGTCGTGCGCAA
CGCGATCCGCCGCAAGGAGCGCGCGGAAGACGAGAGCCGGCGGCCGGCGGCCGTCGACACGTGGCTCGCGAAATTCGCGA
GCGCGGCGGGGAAGGCGGACAAGGGGTGA

Upstream 100 bases:

>100_bases
GGCGCGCGCCTCATCGCCGCGCGCCGTCGTGCGGCGGTTTCGCTTTCGTTTTCCCGCCGTGCGCGGCACGGCAACCCGAC
TCTTACCGGCACGACACCCG

Downstream 100 bases:

>100_bases
CGAGGTTCCGATGCGATGACGTGACGCGCAACGAAAAAGGGACGCTGCGAAGCGTCCCTTGGCTTTGGGTACGGCGTGCC
GGGTGGCGGCGTTACACGCG

Product: FAD dependent oxidoreductase

Products: NA

Alternate protein names: Gamma-Glu-Put oxidase; Gamma-glutamylputrescine oxidase [H]

Number of amino acids: Translated: 462; Mature: 462

Protein sequence:

>462_residues
MRPFWIEQALFNDGDLAPALQGATQADVCIVGGGFTGLWTAIQAKQQNPALDIVILEADLCGAGASGRNGGCLLTWSAKF
LTLRRLFGEAEAIRLVKASEAAVQHIADFCRAHRIDAELRLDGTLYTATSRAQIGTLAPVLDALAACGIHSYEPLPAADV
ARRSGSVRNLDGVYSLIAATVHPGKLARGLRRVALDMGIRIHERTPLVDFTPGQPAVVRTPSGSVRAGKLVFAINAWMAS
RFPQFERTIAVVSSDMVITEKCPELLEQTGLVDGVSVLDSRIFVYYYRTTADGRLMLGKGGNTFSWRSRIAPVFDRRSPY
EAQLTRSLREFFPSLAGVPITASWNGPSDRSVTGFPFFGRLDDAPNVFYGFGYSGNGVGPTYMGGQILSSLVLGLDNAWT
RSPIVRGPLGHFPPEPIRYVGAHVVRNAIRRKERAEDESRRPAAVDTWLAKFASAAGKADKG

Sequences:

>Translated_462_residues
MRPFWIEQALFNDGDLAPALQGATQADVCIVGGGFTGLWTAIQAKQQNPALDIVILEADLCGAGASGRNGGCLLTWSAKF
LTLRRLFGEAEAIRLVKASEAAVQHIADFCRAHRIDAELRLDGTLYTATSRAQIGTLAPVLDALAACGIHSYEPLPAADV
ARRSGSVRNLDGVYSLIAATVHPGKLARGLRRVALDMGIRIHERTPLVDFTPGQPAVVRTPSGSVRAGKLVFAINAWMAS
RFPQFERTIAVVSSDMVITEKCPELLEQTGLVDGVSVLDSRIFVYYYRTTADGRLMLGKGGNTFSWRSRIAPVFDRRSPY
EAQLTRSLREFFPSLAGVPITASWNGPSDRSVTGFPFFGRLDDAPNVFYGFGYSGNGVGPTYMGGQILSSLVLGLDNAWT
RSPIVRGPLGHFPPEPIRYVGAHVVRNAIRRKERAEDESRRPAAVDTWLAKFASAAGKADKG
>Mature_462_residues
MRPFWIEQALFNDGDLAPALQGATQADVCIVGGGFTGLWTAIQAKQQNPALDIVILEADLCGAGASGRNGGCLLTWSAKF
LTLRRLFGEAEAIRLVKASEAAVQHIADFCRAHRIDAELRLDGTLYTATSRAQIGTLAPVLDALAACGIHSYEPLPAADV
ARRSGSVRNLDGVYSLIAATVHPGKLARGLRRVALDMGIRIHERTPLVDFTPGQPAVVRTPSGSVRAGKLVFAINAWMAS
RFPQFERTIAVVSSDMVITEKCPELLEQTGLVDGVSVLDSRIFVYYYRTTADGRLMLGKGGNTFSWRSRIAPVFDRRSPY
EAQLTRSLREFFPSLAGVPITASWNGPSDRSVTGFPFFGRLDDAPNVFYGFGYSGNGVGPTYMGGQILSSLVLGLDNAWT
RSPIVRGPLGHFPPEPIRYVGAHVVRNAIRRKERAEDESRRPAAVDTWLAKFASAAGKADKG

Specific function: Involved in the breakdown of putrescine via the oxidation of L-glutamylputrescine [H]

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787559, Length=379, Percent_Identity=30.3430079155673, Blast_Score=155, Evalue=7e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 49962; Mature: 49962

Theoretical pI: Translated: 9.48; Mature: 9.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPFWIEQALFNDGDLAPALQGATQADVCIVGGGFTGLWTAIQAKQQNPALDIVILEADL
CCCCCHHHHHCCCCCCCHHHCCCCCCCEEEECCCHHHHHHHHHHHCCCCCEEEEEEEECC
CGAGASGRNGGCLLTWSAKFLTLRRLFGEAEAIRLVKASEAAVQHIADFCRAHRIDAELR
CCCCCCCCCCCEEEEEHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
LDGTLYTATSRAQIGTLAPVLDALAACGIHSYEPLPAADVARRSGSVRNLDGVYSLIAAT
ECCEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHH
VHPGKLARGLRRVALDMGIRIHERTPLVDFTPGQPAVVRTPSGSVRAGKLVFAINAWMAS
CCCHHHHHHHHHHHHHCCCEEECCCCEEEECCCCCEEEECCCCCCCCCEEEEEEEHHHHH
RFPQFERTIAVVSSDMVITEKCPELLEQTGLVDGVSVLDSRIFVYYYRTTADGRLMLGKG
CCCHHHHHHHHHCCCEEEHHHHHHHHHHCCCCHHHHHHCCEEEEEEEEECCCCEEEEECC
GNTFSWRSRIAPVFDRRSPYEAQLTRSLREFFPSLAGVPITASWNGPSDRSVTGFPFFGR
CCCCHHHHHCCHHHCCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCEECCCCCCC
LDDAPNVFYGFGYSGNGVGPTYMGGQILSSLVLGLDNAWTRSPIVRGPLGHFPPEPIRYV
CCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHH
GAHVVRNAIRRKERAEDESRRPAAVDTWLAKFASAAGKADKG
HHHHHHHHHHHHHHCCHHHCCCCHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MRPFWIEQALFNDGDLAPALQGATQADVCIVGGGFTGLWTAIQAKQQNPALDIVILEADL
CCCCCHHHHHCCCCCCCHHHCCCCCCCEEEECCCHHHHHHHHHHHCCCCCEEEEEEEECC
CGAGASGRNGGCLLTWSAKFLTLRRLFGEAEAIRLVKASEAAVQHIADFCRAHRIDAELR
CCCCCCCCCCCEEEEEHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
LDGTLYTATSRAQIGTLAPVLDALAACGIHSYEPLPAADVARRSGSVRNLDGVYSLIAAT
ECCEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHH
VHPGKLARGLRRVALDMGIRIHERTPLVDFTPGQPAVVRTPSGSVRAGKLVFAINAWMAS
CCCHHHHHHHHHHHHHCCCEEECCCCEEEECCCCCEEEECCCCCCCCCEEEEEEEHHHHH
RFPQFERTIAVVSSDMVITEKCPELLEQTGLVDGVSVLDSRIFVYYYRTTADGRLMLGKG
CCCHHHHHHHHHCCCEEEHHHHHHHHHHCCCCHHHHHHCCEEEEEEEEECCCCEEEEECC
GNTFSWRSRIAPVFDRRSPYEAQLTRSLREFFPSLAGVPITASWNGPSDRSVTGFPFFGR
CCCCHHHHHCCHHHCCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCEECCCCCCC
LDDAPNVFYGFGYSGNGVGPTYMGGQILSSLVLGLDNAWTRSPIVRGPLGHFPPEPIRYV
CCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHH
GAHVVRNAIRRKERAEDESRRPAAVDTWLAKFASAAGKADKG
HHHHHHHHHHHHHHCCHHHCCCCHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 1840553; 7984428 [H]