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The map label for this gene is yhjG [H]

Identifier: 115350947

GI number: 115350947

Start: 982163

End: 983815

Strand: Direct

Name: yhjG [H]

Synonym: Bamb_0893

Alternate gene names: 115350947

Gene position: 982163-983815 (Clockwise)

Preceding gene: 115350945

Following gene: 115350952

Centisome position: 27.62

GC content: 71.45

Gene sequence:

>1653_bases
ATGGCTGACACACTGCTCGACATCCCGCCCGTGCTGATCGTCGGCGCAGGCCCGACCGGCCTTGCCGCCGCGATGAGTCT
CGCGCGTGCCCGCGTGCCGGTACGCATCATCGACCGGCTCACCACGCCCGCGCCGTATTCGCGTGCGATCGGCATCCAGG
CGCGCACGCTCGAAATGCTGGAGCAGCATCGCGCGGTCGAACCGTTTCTCGTGCTGGGGCATCGCGCGCACGCGGCCGCG
CTGCATGCGGGCGGCCGCGTGATCGCGCGGCTCGATTTCGATCCGCTGCAAACGCGCTATCCGTACCTGCTGTTTCTCGA
CCAGAGCATCACCGAGCAGCTGCTCGCCGAGCATCTGGCCGGCCTCGGCGTGACGGTCGAACGCGGCGTCACGCTCACGG
CGTGCGACGCGGGCGGCACGTCGCTCGACGTGACGATCCGCGGCGCCGACGGGCGTGAACGATCGTTCGCGCCGTCCTAC
CTGATCGCCGCCGACGGCGCGCACAGCACGGTGCGCCATCTGCTTGGCCTCGGCTTCGCCGGGCAAGCGTTCGAGCAGAC
CTTCCTGCTCGCCGATTTCTCGGCGATACCCGACTGGCCGGAAGAGGAGATCCACCTGTTCACGACGCCCGACGGCATCG
CGGGGCTGTTCCCGATGGGCGGCGGCCGCTACCGGCTCGTCGCCGACCGGCCGCCGGGCGATGCATCTCCCGATGCGCCG
CCACCGTCGCTCGCGCAATGCGAGGCGATCGTGCGCGCCCGTGTCGGCGCGTCGATCGCGCCGAGCGACCTCGCGTGGTC
GTCGTATTTCCGGCTGCACAGCCGGATGGTCGCCCGGCTGCGTCACGGGCGCGTGTTCTTCGCGGGCGACGCCGCGCACG
TTCACAGCCCGGCAGGCGCGCAGGGCATGAACACGGGCATGCAGGAAGCGTTCAATCTCGGCTGGAAGCTCGCGCGCGTG
CTTGGCGGCGGCACGCCGGAGCGGCTGCTCGACACCTATCACGCGGAGCGCCATCCGATCGAGCGTGACGTATTGCGGCA
GACCGGCCTCGTCACGCAGATCGTCGAAGCCGATCATGGCGCGATGAAGCTGCTGCGCGATCACGTCGTGCCGCTGCTGG
CGACGTTCGGGCCCGTGCGCGACGCGGTGCGGCGCACCGTCAGCGAGCTTGGCGTGCAATACCGGAAAAGCCCGCTCACG
CTCGAGCGCGTGCTCGACGGCGGGCCGCGCGCCGGCGAGCGTGCGCCGGACGCGCTCGTACATGTGGTCGACGGGCCGCT
CGGGCGGGCACCCGGCACCGCGCGGCTATACGACCTGCACGACCCGGCGAGCTTCACGCTGCTGTTGCTCGAGGAGCCGG
CAGGCGTCGAAGCGGGCGATGCGAACGACGTGCCGCCGATGCCCGCGGATGCGCGGGCGCTCGCGCAGGGGCTGGAGCGG
ATCATGCCGGGCGCGGTGCGGATTTGGCGCGTCGCCGATGCCGAAAGCGACGGCGAGGACGGGCTGGCGCAGGCATACGG
CCGCTCGCGGCCGTCGTTCTACCTGCTGCGGCCCGATGGTTACATCGCGGCGCGCGGCCGTACGGCCACCGACGCGAACG
CGCTGCTGCGCCACTGCGAAAGCTGGTTCGCGGGCATGTCGCTGCCCGCGTAG

Upstream 100 bases:

>100_bases
TCCCACACTGCGCGCGGGCGCCCCAGATTGGCCGGTCGGCATATGGCCCCGTCACGCGGAGCGGCGTAGACTTGCAGGAT
CGCGTGTCATCGAGGCGCTC

Downstream 100 bases:

>100_bases
CGGGGCGCTCAGGCGGAGACTGCGGCCGGCACGAGCGACGCGCGGTGCGCGGCGATCGCGGCGAGCACGATCGGCGCCGC
GCGTTGGGCGGCTTCGCTCG

Product: FAD-binding monooxygenase

Products: H2O; 3-(2,3-dihydroxyphenyl)propionate; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 550; Mature: 549

Protein sequence:

>550_residues
MADTLLDIPPVLIVGAGPTGLAAAMSLARARVPVRIIDRLTTPAPYSRAIGIQARTLEMLEQHRAVEPFLVLGHRAHAAA
LHAGGRVIARLDFDPLQTRYPYLLFLDQSITEQLLAEHLAGLGVTVERGVTLTACDAGGTSLDVTIRGADGRERSFAPSY
LIAADGAHSTVRHLLGLGFAGQAFEQTFLLADFSAIPDWPEEEIHLFTTPDGIAGLFPMGGGRYRLVADRPPGDASPDAP
PPSLAQCEAIVRARVGASIAPSDLAWSSYFRLHSRMVARLRHGRVFFAGDAAHVHSPAGAQGMNTGMQEAFNLGWKLARV
LGGGTPERLLDTYHAERHPIERDVLRQTGLVTQIVEADHGAMKLLRDHVVPLLATFGPVRDAVRRTVSELGVQYRKSPLT
LERVLDGGPRAGERAPDALVHVVDGPLGRAPGTARLYDLHDPASFTLLLLEEPAGVEAGDANDVPPMPADARALAQGLER
IMPGAVRIWRVADAESDGEDGLAQAYGRSRPSFYLLRPDGYIAARGRTATDANALLRHCESWFAGMSLPA

Sequences:

>Translated_550_residues
MADTLLDIPPVLIVGAGPTGLAAAMSLARARVPVRIIDRLTTPAPYSRAIGIQARTLEMLEQHRAVEPFLVLGHRAHAAA
LHAGGRVIARLDFDPLQTRYPYLLFLDQSITEQLLAEHLAGLGVTVERGVTLTACDAGGTSLDVTIRGADGRERSFAPSY
LIAADGAHSTVRHLLGLGFAGQAFEQTFLLADFSAIPDWPEEEIHLFTTPDGIAGLFPMGGGRYRLVADRPPGDASPDAP
PPSLAQCEAIVRARVGASIAPSDLAWSSYFRLHSRMVARLRHGRVFFAGDAAHVHSPAGAQGMNTGMQEAFNLGWKLARV
LGGGTPERLLDTYHAERHPIERDVLRQTGLVTQIVEADHGAMKLLRDHVVPLLATFGPVRDAVRRTVSELGVQYRKSPLT
LERVLDGGPRAGERAPDALVHVVDGPLGRAPGTARLYDLHDPASFTLLLLEEPAGVEAGDANDVPPMPADARALAQGLER
IMPGAVRIWRVADAESDGEDGLAQAYGRSRPSFYLLRPDGYIAARGRTATDANALLRHCESWFAGMSLPA
>Mature_549_residues
ADTLLDIPPVLIVGAGPTGLAAAMSLARARVPVRIIDRLTTPAPYSRAIGIQARTLEMLEQHRAVEPFLVLGHRAHAAAL
HAGGRVIARLDFDPLQTRYPYLLFLDQSITEQLLAEHLAGLGVTVERGVTLTACDAGGTSLDVTIRGADGRERSFAPSYL
IAADGAHSTVRHLLGLGFAGQAFEQTFLLADFSAIPDWPEEEIHLFTTPDGIAGLFPMGGGRYRLVADRPPGDASPDAPP
PSLAQCEAIVRARVGASIAPSDLAWSSYFRLHSRMVARLRHGRVFFAGDAAHVHSPAGAQGMNTGMQEAFNLGWKLARVL
GGGTPERLLDTYHAERHPIERDVLRQTGLVTQIVEADHGAMKLLRDHVVPLLATFGPVRDAVRRTVSELGVQYRKSPLTL
ERVLDGGPRAGERAPDALVHVVDGPLGRAPGTARLYDLHDPASFTLLLLEEPAGVEAGDANDVPPMPADARALAQGLERI
MPGAVRIWRVADAESDGEDGLAQAYGRSRPSFYLLRPDGYIAARGRTATDANALLRHCESWFAGMSLPA

Specific function: 3-hydroxyphenylpropionate degradation. [C]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pheA/tfdB FAD monooxygenase family [H]

Homologues:

Organism=Escherichia coli, GI1786543, Length=379, Percent_Identity=27.1767810026385, Blast_Score=108, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: 1.14.13.- [C]

Molecular weight: Translated: 59004; Mature: 58873

Theoretical pI: Translated: 6.56; Mature: 6.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADTLLDIPPVLIVGAGPTGLAAAMSLARARVPVRIIDRLTTPAPYSRAIGIQARTLEML
CCCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCHHHHHHCCCCCCCHHHCCCHHHHHHHH
EQHRAVEPFLVLGHRAHAAALHAGGRVIARLDFDPLQTRYPYLLFLDQSITEQLLAEHLA
HHHCCCCCCEEECCCHHHHHHHCCCEEEEEECCCCHHCCCCEEEEECHHHHHHHHHHHHH
GLGVTVERGVTLTACDAGGTSLDVTIRGADGRERSFAPSYLIAADGAHSTVRHLLGLGFA
CCCEEEECCEEEEEECCCCCEEEEEEECCCCCCCCCCCCEEEEECCCHHHHHHHHHCCCC
GQAFEQTFLLADFSAIPDWPEEEIHLFTTPDGIAGLFPMGGGRYRLVADRPPGDASPDAP
CHHHHHHHHEEEHHCCCCCCCCCEEEEECCCCCCCCCCCCCCEEEEEECCCCCCCCCCCC
PPSLAQCEAIVRARVGASIAPSDLAWSSYFRLHSRMVARLRHGRVFFAGDAAHVHSPAGA
CCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEECCHHHCCCCCCC
QGMNTGMQEAFNLGWKLARVLGGGTPERLLDTYHAERHPIERDVLRQTGLVTQIVEADHG
CCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHCHHHHHHHCCHH
AMKLLRDHVVPLLATFGPVRDAVRRTVSELGVQYRKSPLTLERVLDGGPRAGERAPDALV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCCCCCEE
HVVDGPLGRAPGTARLYDLHDPASFTLLLLEEPAGVEAGDANDVPPMPADARALAQGLER
EEECCCCCCCCCCCEEEECCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHH
IMPGAVRIWRVADAESDGEDGLAQAYGRSRPSFYLLRPDGYIAARGRTATDANALLRHCE
HCCCCEEEEEEECCCCCCCCHHHHHHCCCCCCEEEECCCCEEEECCCCCCCHHHHHHHHH
SWFAGMSLPA
HHHCCCCCCC
>Mature Secondary Structure 
ADTLLDIPPVLIVGAGPTGLAAAMSLARARVPVRIIDRLTTPAPYSRAIGIQARTLEML
CCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCHHHHHHCCCCCCCHHHCCCHHHHHHHH
EQHRAVEPFLVLGHRAHAAALHAGGRVIARLDFDPLQTRYPYLLFLDQSITEQLLAEHLA
HHHCCCCCCEEECCCHHHHHHHCCCEEEEEECCCCHHCCCCEEEEECHHHHHHHHHHHHH
GLGVTVERGVTLTACDAGGTSLDVTIRGADGRERSFAPSYLIAADGAHSTVRHLLGLGFA
CCCEEEECCEEEEEECCCCCEEEEEEECCCCCCCCCCCCEEEEECCCHHHHHHHHHCCCC
GQAFEQTFLLADFSAIPDWPEEEIHLFTTPDGIAGLFPMGGGRYRLVADRPPGDASPDAP
CHHHHHHHHEEEHHCCCCCCCCCEEEEECCCCCCCCCCCCCCEEEEEECCCCCCCCCCCC
PPSLAQCEAIVRARVGASIAPSDLAWSSYFRLHSRMVARLRHGRVFFAGDAAHVHSPAGA
CCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEECCHHHCCCCCCC
QGMNTGMQEAFNLGWKLARVLGGGTPERLLDTYHAERHPIERDVLRQTGLVTQIVEADHG
CCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHCHHHHHHHCCHH
AMKLLRDHVVPLLATFGPVRDAVRRTVSELGVQYRKSPLTLERVLDGGPRAGERAPDALV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCCCCCEE
HVVDGPLGRAPGTARLYDLHDPASFTLLLLEEPAGVEAGDANDVPPMPADARALAQGLER
EEECCCCCCCCCCCEEEECCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHH
IMPGAVRIWRVADAESDGEDGLAQAYGRSRPSFYLLRPDGYIAARGRTATDANALLRHCE
HCCCCEEEEEEECCCCCCCCHHHHHHCCCCCCEEEECCCCEEEECCCCCCCHHHHHHHHH
SWFAGMSLPA
HHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-(3-hydroxyphenyl)propionate; NADH; O2 [C]

Specific reaction: 3-(3-hydroxyphenyl)propionate + NADH + O2 = H2O + 3-(2,3-dihydroxyphenyl)propionate + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]