Click here to switch to the map view.

The map label for this gene is citH [H]

Identifier: 115350945

GI number: 115350945

Start: 978824

End: 980119

Strand: Direct

Name: citH [H]

Synonym: Bamb_0891

Alternate gene names: 115350945

Gene position: 978824-980119 (Clockwise)

Preceding gene: 115350943

Following gene: 115350947

Centisome position: 27.52

GC content: 65.9

Gene sequence:

>1296_bases
ATGTCCACAGCAACACAAGCCATCGCGCAGGAATCGAAAGTCCGCACCGTGTTCCGGGTCGTCAGCGGCAACTTCCTGGA
AATGTACGACTTCATGGTCTATGGGTATTACGCGTCCGCGATCGCGAAAACCTACTTTCCGAGCGGCAACGCGTTCGCGT
CGCTGATGCTGTCGCTGTCGGTGTTCGGCGCGGGCTTCCTGATGCGCCCGGTCGGCGCGATCGTGCTCGGCGCGTACATC
GACCATCACGGCCGCCGCAAGGGGCTGATCCTGACGCTCGGGCTGATGGCGCTCGGCACGCTGACGGTGGCCGGGGTCCC
CGGCTACGCGACGATCGGCGTGCTCGCGCCGGCGCTCGTGCTGCTCGGCCGGTTGCTGCAGGGCTTTTCCGCGGGCGTCG
AGCTGGGCGGCGTGTCGGTCTACCTGTCGGAGATCGCGACGAAGGGCCACAAGGGGTTCTATACGTCGTGGCAATCGGGC
AGCCAGCAGGTGGCCGTCGTGTTCGCCGCGTTTGTCGGCGTGCTGCTGAACCGCGTGCTGCCGGCCGAGGACATGAGCGC
GTGGGGCTGGCGCATTCCGTTCCTGATCGGCTGCCTGATCGTACCGTTCCTGTTCCTGATCCGTCGTTCGCTGAAGGAAA
CCGACGAGTTCCTCGCGAAGCGTCATCGTCCGACGATGGGCGAGATCATGCGTTCGATGCTCGAGAACTGGGGTGTCGTG
ATCGCCGGCATGGGGATGGTGATCATGACGACGGTGTCGTTCTACATGATCACCGCATACACGCCGACGTTCGGCAAGGA
AGTGCTGCACCTGTCGTCGCTCGACGCACTCGTCGTGACCGTCTGCGTCGGCCTGTCCAACCTCGTGTGGCTGCCGCTGT
CCGGCGCGCTGTCGGATCGCATCGGCCGCCGGCCGGTGCTGATCGCGTTCACGGTGCTCACGCTGCTGTCGGCCTATCCG
GCCGTGCTGTGGCTCGTGGCCGAACCGTCGTTCCTGCGGCTGCTGGCGGTCGAGCTGTGGCTGTCGTTCCTGTACGGTTC
CTACAACGGCGCGATGGTCGTCGCGCTGACCGAAGTGATGCCGGTCGACGTGCGCACCGCCGGCTTCTCGCTCGCATACA
GCCTGGCGACGACGATCGGCGGCTTCACGCCCGCGATCTCGACGTTGCTGATCCACCAGACGGGCAACAAGGCCGCACCG
GGGCTGTGGCTGAGCGTCGCGGCGCTTTGCGGGCTGATCGCGACGCTCGTGCTGTATCGCACGCGCGAGGCGCGCAACCA
GTACAAGGCGGCCTGA

Upstream 100 bases:

>100_bases
CGGCTTGGCGTGGCATCATCCGCGAACCATGCCGGTGCGTCGCGCGTCATCCGATCCGTGCGGCGCGCCGATGTCGCGTC
ATCCGAATCGAGAGAGCAAC

Downstream 100 bases:

>100_bases
CGCCGCCTGCACCGCGCCACGTCGGGTGAAACAAGGAGCGCGCGGCGCTCCCTTTTCTCATCGGCTTTCGGAATTCACGC
GGCGCGCAGCGCGTCGGCGA

Product: general substrate transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Citrate carrier protein; Citrate transporter [H]

Number of amino acids: Translated: 431; Mature: 430

Protein sequence:

>431_residues
MSTATQAIAQESKVRTVFRVVSGNFLEMYDFMVYGYYASAIAKTYFPSGNAFASLMLSLSVFGAGFLMRPVGAIVLGAYI
DHHGRRKGLILTLGLMALGTLTVAGVPGYATIGVLAPALVLLGRLLQGFSAGVELGGVSVYLSEIATKGHKGFYTSWQSG
SQQVAVVFAAFVGVLLNRVLPAEDMSAWGWRIPFLIGCLIVPFLFLIRRSLKETDEFLAKRHRPTMGEIMRSMLENWGVV
IAGMGMVIMTTVSFYMITAYTPTFGKEVLHLSSLDALVVTVCVGLSNLVWLPLSGALSDRIGRRPVLIAFTVLTLLSAYP
AVLWLVAEPSFLRLLAVELWLSFLYGSYNGAMVVALTEVMPVDVRTAGFSLAYSLATTIGGFTPAISTLLIHQTGNKAAP
GLWLSVAALCGLIATLVLYRTREARNQYKAA

Sequences:

>Translated_431_residues
MSTATQAIAQESKVRTVFRVVSGNFLEMYDFMVYGYYASAIAKTYFPSGNAFASLMLSLSVFGAGFLMRPVGAIVLGAYI
DHHGRRKGLILTLGLMALGTLTVAGVPGYATIGVLAPALVLLGRLLQGFSAGVELGGVSVYLSEIATKGHKGFYTSWQSG
SQQVAVVFAAFVGVLLNRVLPAEDMSAWGWRIPFLIGCLIVPFLFLIRRSLKETDEFLAKRHRPTMGEIMRSMLENWGVV
IAGMGMVIMTTVSFYMITAYTPTFGKEVLHLSSLDALVVTVCVGLSNLVWLPLSGALSDRIGRRPVLIAFTVLTLLSAYP
AVLWLVAEPSFLRLLAVELWLSFLYGSYNGAMVVALTEVMPVDVRTAGFSLAYSLATTIGGFTPAISTLLIHQTGNKAAP
GLWLSVAALCGLIATLVLYRTREARNQYKAA
>Mature_430_residues
STATQAIAQESKVRTVFRVVSGNFLEMYDFMVYGYYASAIAKTYFPSGNAFASLMLSLSVFGAGFLMRPVGAIVLGAYID
HHGRRKGLILTLGLMALGTLTVAGVPGYATIGVLAPALVLLGRLLQGFSAGVELGGVSVYLSEIATKGHKGFYTSWQSGS
QQVAVVFAAFVGVLLNRVLPAEDMSAWGWRIPFLIGCLIVPFLFLIRRSLKETDEFLAKRHRPTMGEIMRSMLENWGVVI
AGMGMVIMTTVSFYMITAYTPTFGKEVLHLSSLDALVVTVCVGLSNLVWLPLSGALSDRIGRRPVLIAFTVLTLLSAYPA
VLWLVAEPSFLRLLAVELWLSFLYGSYNGAMVVALTEVMPVDVRTAGFSLAYSLATTIGGFTPAISTLLIHQTGNKAAPG
LWLSVAALCGLIATLVLYRTREARNQYKAA

Specific function: Uptake of citrate across the boundary membrane with the concomitant transport of protons into the cell (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=428, Percent_Identity=29.6728971962617, Blast_Score=212, Evalue=4e-56,
Organism=Escherichia coli, GI1788942, Length=428, Percent_Identity=33.1775700934579, Blast_Score=186, Evalue=3e-48,
Organism=Escherichia coli, GI1788292, Length=415, Percent_Identity=27.710843373494, Blast_Score=164, Evalue=1e-41,
Organism=Escherichia coli, GI1789941, Length=421, Percent_Identity=30.4038004750594, Blast_Score=139, Evalue=3e-34,
Organism=Escherichia coli, GI87082231, Length=226, Percent_Identity=27.4336283185841, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 46484; Mature: 46352

Theoretical pI: Translated: 9.76; Mature: 9.76

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1 ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHCCCC
>Mature Secondary Structure 
STATQAIAQESKVRTVFRVVSGNFLEMYDFMVYGYYASAIAKTYFPSGNAFASLMLSLS
CHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
VFGAGFLMRPVGAIVLGAYIDHHGRRKGLILTLGLMALGTLTVAGVPGYATIGVLAPALV
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
LLGRLLQGFSAGVELGGVSVYLSEIATKGHKGFYTSWQSGSQQVAVVFAAFVGVLLNRVL
HHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCC
PAEDMSAWGWRIPFLIGCLIVPFLFLIRRSLKETDEFLAKRHRPTMGEIMRSMLENWGVV
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCEE
IAGMGMVIMTTVSFYMITAYTPTFGKEVLHLSSLDALVVTVCVGLSNLVWLPLSGALSDR
EHHHHHHHHHHHHHHHHHEECCHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCHHHHHH
IGRRPVLIAFTVLTLLSAYPAVLWLVAEPSFLRLLAVELWLSFLYGSYNGAMVVALTEVM
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEHHC
PVDVRTAGFSLAYSLATTIGGFTPAISTLLIHQTGNKAAPGLWLSVAALCGLIATLVLYR
CCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
TREARNQYKAA
HHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2186908 [H]