Click here to switch to the map view.

The map label for this gene is appB [H]

Identifier: 116249443

GI number: 116249443

Start: 852562

End: 853548

Strand: Direct

Name: appB [H]

Synonym: pRL120781

Alternate gene names: 116249443

Gene position: 852562-853548 (Clockwise)

Preceding gene: 116249442

Following gene: 116249444

Centisome position: 97.99

GC content: 58.76

Gene sequence:

>987_bases
ATGCTGGTCTTTATCGCCAAACGCTTCCTATGGATGATTCCATCGCTGTTTGCCGTCAGCTTCCTGGCTTTCGTGCTGAT
CCAGCTGCCGCCGGGCGATTATGTCACGACCTATATTGCGACGCTGGCAGCATCCAACGAAATCGTCGATCAGAACACGG
CGGCGCAATTGCGCGAGCGCTTCGGCCTCGGCGACCCGATGCTCGTCCAATATTTCAAATGGATGTGGGGCATTCTCACG
CGCGGCGATTTCGGCATCTCCTTCGAATGGCAGCAGCCGGTCTCGGACCTGATCTGGGAGCGCATGGCGCTGACGCTCGT
TCTGGCCCTTTCGACATTGATCGCCACCTGGGCGATCGCGCTGCCGATCGGCGTCTATTCCGCGGTGCGCAAATATTCGA
TCGGCGACTATTTCTTCACCGCCTTCACCTTTTTCGGCCTGGCTGTTCCATCCTTTCTGCTGGCGCTGGTGCTGATGTAC
ATCGCGGCGGTCGAATTCGGACAGGATGTCGGCGGGCTCTTTTCGCCGGAATATGAGAACGCGTCCTGGAGCTTCGCCAA
GATGGTCGACCTCTTCTCGCATCTCTGGCTTCCCGTCATCATCCTTGCCGTCTCCTCGACGGCGAGCCTCATCCGCGTCA
TGCGCGCCAACATGCTCGATGAATTGCCGAAGCCTTACGTGACCACGGCACGGGCAAAAGGGCTCTCTGAGTTCCGGCTG
CTGATGAAATATCCTCTGATGATCGCGCTCAATCCGTTCATCTCGACGATTGCCTGGCTGCTGCCCAATCTCATTTCCGG
CTCGGTCGTCGTCGCCATCGTCCTCAATCTGCCGACGGCCGCACCTTTGCTGCTGCAGGCGCTGATGGCCCAGGACATGT
ATCTCGCCGGCGCCTTCGTTCTGCTGATCTGCGCGCTGACGCTGATAGGCTCTCTGATCAGCGATATCCTGCTTGCGCTG
GTCGATCCCCGCATCCGGTTGGAATAG

Upstream 100 bases:

>100_bases
TCAGGTACGGCGCTTTCTTTCAGTACGCCCTCCGAGATGCCTTGCCGGTCCTGACCGCCGACAAGGCCCGGGAGGCGGCG
TTCAATGCAAGAGGCGCCTC

Downstream 100 bases:

>100_bases
GAGCCTCACGTGGCCGACATCCCCATTACAAATATTCAGCCGGATCGCGCCGCCGTCGCGTCGCAGTGGCAGCTCATCTG
GTGGGCTTTCCGCCGGCACC

Product: putative permease component of ABC transporter

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 328; Mature: 328

Protein sequence:

>328_residues
MLVFIAKRFLWMIPSLFAVSFLAFVLIQLPPGDYVTTYIATLAASNEIVDQNTAAQLRERFGLGDPMLVQYFKWMWGILT
RGDFGISFEWQQPVSDLIWERMALTLVLALSTLIATWAIALPIGVYSAVRKYSIGDYFFTAFTFFGLAVPSFLLALVLMY
IAAVEFGQDVGGLFSPEYENASWSFAKMVDLFSHLWLPVIILAVSSTASLIRVMRANMLDELPKPYVTTARAKGLSEFRL
LMKYPLMIALNPFISTIAWLLPNLISGSVVVAIVLNLPTAAPLLLQALMAQDMYLAGAFVLLICALTLIGSLISDILLAL
VDPRIRLE

Sequences:

>Translated_328_residues
MLVFIAKRFLWMIPSLFAVSFLAFVLIQLPPGDYVTTYIATLAASNEIVDQNTAAQLRERFGLGDPMLVQYFKWMWGILT
RGDFGISFEWQQPVSDLIWERMALTLVLALSTLIATWAIALPIGVYSAVRKYSIGDYFFTAFTFFGLAVPSFLLALVLMY
IAAVEFGQDVGGLFSPEYENASWSFAKMVDLFSHLWLPVIILAVSSTASLIRVMRANMLDELPKPYVTTARAKGLSEFRL
LMKYPLMIALNPFISTIAWLLPNLISGSVVVAIVLNLPTAAPLLLQALMAQDMYLAGAFVLLICALTLIGSLISDILLAL
VDPRIRLE
>Mature_328_residues
MLVFIAKRFLWMIPSLFAVSFLAFVLIQLPPGDYVTTYIATLAASNEIVDQNTAAQLRERFGLGDPMLVQYFKWMWGILT
RGDFGISFEWQQPVSDLIWERMALTLVLALSTLIATWAIALPIGVYSAVRKYSIGDYFFTAFTFFGLAVPSFLLALVLMY
IAAVEFGQDVGGLFSPEYENASWSFAKMVDLFSHLWLPVIILAVSSTASLIRVMRANMLDELPKPYVTTARAKGLSEFRL
LMKYPLMIALNPFISTIAWLLPNLISGSVVVAIVLNLPTAAPLLLQALMAQDMYLAGAFVLLICALTLIGSLISDILLAL
VDPRIRLE

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence, though, unlike OPP, is incapable of transportin

COG id: COG0601

COG function: function code EP; ABC-type dipeptide/oligopeptide/nickel transport systems, permease components

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789965, Length=342, Percent_Identity=28.9473684210526, Blast_Score=152, Evalue=4e-38,
Organism=Escherichia coli, GI1787497, Length=327, Percent_Identity=28.1345565749235, Blast_Score=134, Evalue=6e-33,
Organism=Escherichia coli, GI1787053, Length=330, Percent_Identity=30.3030303030303, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI1789888, Length=326, Percent_Identity=29.7546012269939, Blast_Score=132, Evalue=4e-32,
Organism=Escherichia coli, GI1788504, Length=364, Percent_Identity=27.7472527472527, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1787550, Length=261, Percent_Identity=26.8199233716475, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1787761, Length=322, Percent_Identity=26.7080745341615, Blast_Score=83, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 36440; Mature: 36440

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure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HHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377 [H]