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The map label for this gene is cspA [C]

Identifier: 116249427

GI number: 116249427

Start: 834189

End: 834392

Strand: Reverse

Name: cspA [C]

Synonym: pRL120765

Alternate gene names: 116249427

Gene position: 834392-834189 (Counterclockwise)

Preceding gene: 116249428

Following gene: 116249405

Centisome position: 95.9

GC content: 56.86

Gene sequence:

>204_bases
ATGCCGACAGGTACCGTTAAATTCTTCAATGACGACAAGGGCTTCGGCTTCATCACCCCTGAGGGTGGCGGGCAGGACGT
TTTTGTTCACGTGTCTGCGCTGCAAAGCGGCGGGTCGCTCCGCGAAGGCGACAAGGTCAGCTTCGAAGTTGGACAGGATC
GCAAGACCGGAAAATCGAAGGCTGAGAACGTCTCGACCCTCTGA

Upstream 100 bases:

>100_bases
TTCAAAGAGCCGTCGGCGTGCTAAAGGGTTGATAGAGAAGCCCCGAAGCTATAGTTTCGCCTTCGCAAATCGCTGAACGA
TACAAGGAAATAGTTATCTC

Downstream 100 bases:

>100_bases
TCAAAAATGGCCCAACCTGCATTGAGCGGGTTGGGCTCATCATCACGAGCCGCAGGCGCGAATGCCTTCGTACTCTACAG
CGCGGCGCGATCTTTCAGAT

Product: putative cold shock protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 67; Mature: 66

Protein sequence:

>67_residues
MPTGTVKFFNDDKGFGFITPEGGGQDVFVHVSALQSGGSLREGDKVSFEVGQDRKTGKSKAENVSTL

Sequences:

>Translated_67_residues
MPTGTVKFFNDDKGFGFITPEGGGQDVFVHVSALQSGGSLREGDKVSFEVGQDRKTGKSKAENVSTL
>Mature_66_residues
PTGTVKFFNDDKGFGFITPEGGGQDVFVHVSALQSGGSLREGDKVSFEVGQDRKTGKSKAENVSTL

Specific function: Binds To And Stimulates The Transcription Of The CCAAT- Containing, Cold-Shock-Inducible Promoters Of The H-Ns And Gyra Proteins. Binds Also To The Inverted Repeat 5'-ATTGG-3'. [C]

COG id: COG1278

COG function: function code K; Cold shock proteins

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CSD (cold-shock) domain [H]

Homologues:

Organism=Escherichia coli, GI1789979, Length=67, Percent_Identity=56.7164179104478, Blast_Score=65, Evalue=1e-12,
Organism=Escherichia coli, GI1786841, Length=49, Percent_Identity=61.2244897959184, Blast_Score=64, Evalue=2e-12,
Organism=Escherichia coli, GI1787107, Length=57, Percent_Identity=54.3859649122807, Blast_Score=63, Evalue=3e-12,
Organism=Escherichia coli, GI2367114, Length=67, Percent_Identity=52.2388059701493, Blast_Score=63, Evalue=4e-12,
Organism=Escherichia coli, GI1788126, Length=66, Percent_Identity=53.030303030303, Blast_Score=61, Evalue=2e-11,
Organism=Escherichia coli, GI1787839, Length=50, Percent_Identity=58, Blast_Score=59, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019844
- InterPro:   IPR012156
- InterPro:   IPR011129
- InterPro:   IPR002059
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF00313 CSD [H]

EC number: NA

Molecular weight: Translated: 7094; Mature: 6963

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: PS00352 COLD_SHOCK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTGTVKFFNDDKGFGFITPEGGGQDVFVHVSALQSGGSLREGDKVSFEVGQDRKTGKSK
CCCCCEEEEECCCCCEEECCCCCCCEEEEEEEECCCCCCCCCCCEEEEEECCCCCCCCCC
AENVSTL
CCCCCCC
>Mature Secondary Structure 
PTGTVKFFNDDKGFGFITPEGGGQDVFVHVSALQSGGSLREGDKVSFEVGQDRKTGKSK
CCCCEEEEECCCCCEEECCCCCCCEEEEEEEECCCCCCCCCCCEEEEEECCCCCCCCCC
AENVSTL
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]