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The map label for this gene is yeiE [H]

Identifier: 116249405

GI number: 116249405

Start: 809264

End: 810187

Strand: Reverse

Name: yeiE [H]

Synonym: pRL120743

Alternate gene names: 116249405

Gene position: 810187-809264 (Counterclockwise)

Preceding gene: 116249427

Following gene: 116249404

Centisome position: 93.12

GC content: 60.5

Gene sequence:

>924_bases
ATGACGTTTGAGCAGCTTTCCATTTTTGTCGCGGTTGCCGAACGCGAGCATCTGACCAAAGCCGCCTTTGCGATCGGGCT
GACGCCATCGGCGGTCAGCTCGGCTATCCGCAATCTCGAGACCTCCTATGGCGTCGAGCTTTTCGATCGCGTCGGACGAC
GCATAGAGCTGACATATGAAGGACGGGTGTTTCTGGGGGAGGCTAAAGCGACGCTGGCGCGCGCAAAGGCCGCAGCGCTC
GTCCTCTCCGATCTCGGCGGCTTGCAAAAGGGTGAACTGGTGGTTTTTGCGAGCCAGACGATCGCCAGCTACTGGCTGCC
GGCAATGCTGATGCGTTTCAAGATCCGCTACCCGGGCATTGACCTGAAGCTGATGATTGGCAACACGACCACGGCGGCGA
AAGCGGTACTCGATGGATTGGCCGAAGTCGGTTTCGTCGAAGGCAGTGTTGATGAGCCGGCGCTGCATGTTCAGCCGCTT
GCCGAGGACGAACTCCTTGTCGTCGTCGGTCCACGCCATCCCTGGGCGCGTGGCAAACCAATTGCACCGGCAGACCTGGT
TTCGGGCACAAAATGGGTCATGCGGGAAAAGGGGTCCGGAACCCGGTCGGCTTTCGAGGCGGCGATTTCCATTCTCGGCA
TAGTCCCCGCAGATCTGGCTGTCGCGCTTGAGCTGCCGTCGAACGAGGCGGTTATATCGGCAGCAAGAGAGGGCCTCTGC
GCGACGGTCGTCTCAGGTGCCGTGGCCGCGCCGCTCTTGACGCAAGGTCTGCTGGTAAAGGCGCGCTTCCCTCTGCCGTC
CCGCCAATTTGCGATCCTGCGGCATAAACAAAGGCACTCCAGTCGTGCCTCGCTGGCGCTGGAAACCATTTGCCGCGAGG
ATAAAGCGGCCGAAGTCCAGAATTGGGATGATTGGGCGCTTTAG

Upstream 100 bases:

>100_bases
AGCGAGACCTTGCGGAGGACTGTAATCTCCCACACTCAATTGTCGACTTCCATTGAATAGTATAGCATGGTTCATTCGCT
AAAATCGAACGATTGAATCA

Downstream 100 bases:

>100_bases
GTATGATCCACCGCGCCGGTGGTCTGCTTCACCGTATCATCGATGCGCTTGACATCGGCAGAGTTAAGGCAGAAGCCGCA
CTCGGCCGCGCATGCGCGGC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 307; Mature: 306

Protein sequence:

>307_residues
MTFEQLSIFVAVAEREHLTKAAFAIGLTPSAVSSAIRNLETSYGVELFDRVGRRIELTYEGRVFLGEAKATLARAKAAAL
VLSDLGGLQKGELVVFASQTIASYWLPAMLMRFKIRYPGIDLKLMIGNTTTAAKAVLDGLAEVGFVEGSVDEPALHVQPL
AEDELLVVVGPRHPWARGKPIAPADLVSGTKWVMREKGSGTRSAFEAAISILGIVPADLAVALELPSNEAVISAAREGLC
ATVVSGAVAAPLLTQGLLVKARFPLPSRQFAILRHKQRHSSRASLALETICREDKAAEVQNWDDWAL

Sequences:

>Translated_307_residues
MTFEQLSIFVAVAEREHLTKAAFAIGLTPSAVSSAIRNLETSYGVELFDRVGRRIELTYEGRVFLGEAKATLARAKAAAL
VLSDLGGLQKGELVVFASQTIASYWLPAMLMRFKIRYPGIDLKLMIGNTTTAAKAVLDGLAEVGFVEGSVDEPALHVQPL
AEDELLVVVGPRHPWARGKPIAPADLVSGTKWVMREKGSGTRSAFEAAISILGIVPADLAVALELPSNEAVISAAREGLC
ATVVSGAVAAPLLTQGLLVKARFPLPSRQFAILRHKQRHSSRASLALETICREDKAAEVQNWDDWAL
>Mature_306_residues
TFEQLSIFVAVAEREHLTKAAFAIGLTPSAVSSAIRNLETSYGVELFDRVGRRIELTYEGRVFLGEAKATLARAKAAALV
LSDLGGLQKGELVVFASQTIASYWLPAMLMRFKIRYPGIDLKLMIGNTTTAAKAVLDGLAEVGFVEGSVDEPALHVQPLA
EDELLVVVGPRHPWARGKPIAPADLVSGTKWVMREKGSGTRSAFEAAISILGIVPADLAVALELPSNEAVISAAREGLCA
TVVSGAVAAPLLTQGLLVKARFPLPSRQFAILRHKQRHSSRASLALETICREDKAAEVQNWDDWAL

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788481, Length=285, Percent_Identity=35.0877192982456, Blast_Score=158, Evalue=3e-40,
Organism=Escherichia coli, GI157672245, Length=280, Percent_Identity=31.0714285714286, Blast_Score=101, Evalue=6e-23,
Organism=Escherichia coli, GI1787806, Length=247, Percent_Identity=28.7449392712551, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1788748, Length=305, Percent_Identity=27.5409836065574, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI145693105, Length=202, Percent_Identity=24.2574257425743, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI87082132, Length=217, Percent_Identity=28.110599078341, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1786401, Length=185, Percent_Identity=28.1081081081081, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI1790399, Length=212, Percent_Identity=26.4150943396226, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1788887, Length=193, Percent_Identity=31.0880829015544, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1790208, Length=148, Percent_Identity=33.1081081081081, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1788706, Length=170, Percent_Identity=30, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1788296, Length=231, Percent_Identity=29.004329004329, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787530, Length=264, Percent_Identity=27.2727272727273, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1789173, Length=169, Percent_Identity=31.9526627218935, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI145693193, Length=190, Percent_Identity=29.4736842105263, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1787879, Length=187, Percent_Identity=25.668449197861, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32964; Mature: 32832

Theoretical pI: Translated: 7.63; Mature: 7.63

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFEQLSIFVAVAEREHLTKAAFAIGLTPSAVSSAIRNLETSYGVELFDRVGRRIELTYE
CCHHHEEEEEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHCCCEEEEEEC
GRVFLGEAKATLARAKAAALVLSDLGGLQKGELVVFASQTIASYWLPAMLMRFKIRYPGI
CEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECHHHHHHHHHHHHHHHEEECCCC
DLKLMIGNTTTAAKAVLDGLAEVGFVEGSVDEPALHVQPLAEDELLVVVGPRHPWARGKP
EEEEEECCCHHHHHHHHHHHHHHCEECCCCCCCCEEEECCCCCCEEEEECCCCCCCCCCC
IAPADLVSGTKWVMREKGSGTRSAFEAAISILGIVPADLAVALELPSNEAVISAAREGLC
CCCHHHHCCCHHHHCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHCCHH
ATVVSGAVAAPLLTQGLLVKARFPLPSRQFAILRHKQRHSSRASLALETICREDKAAEVQ
HHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
NWDDWAL
CCCCCCC
>Mature Secondary Structure 
TFEQLSIFVAVAEREHLTKAAFAIGLTPSAVSSAIRNLETSYGVELFDRVGRRIELTYE
CHHHEEEEEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHCCCEEEEEEC
GRVFLGEAKATLARAKAAALVLSDLGGLQKGELVVFASQTIASYWLPAMLMRFKIRYPGI
CEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECHHHHHHHHHHHHHHHEEECCCC
DLKLMIGNTTTAAKAVLDGLAEVGFVEGSVDEPALHVQPLAEDELLVVVGPRHPWARGKP
EEEEEECCCHHHHHHHHHHHHHHCEECCCCCCCCEEEECCCCCCEEEEECCCCCCCCCCC
IAPADLVSGTKWVMREKGSGTRSAFEAAISILGIVPADLAVALELPSNEAVISAAREGLC
CCCHHHHCCCHHHHCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHCCHH
ATVVSGAVAAPLLTQGLLVKARFPLPSRQFAILRHKQRHSSRASLALETICREDKAAEVQ
HHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
NWDDWAL
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]