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The map label for this gene is hutC [H]

Identifier: 116249410

GI number: 116249410

Start: 814613

End: 815380

Strand: Direct

Name: hutC [H]

Synonym: pRL120748

Alternate gene names: 116249410

Gene position: 814613-815380 (Clockwise)

Preceding gene: 116249409

Following gene: 116249411

Centisome position: 93.63

GC content: 60.55

Gene sequence:

>768_bases
ATGACGAAAGCGGTCAAGCCGGCCGCCGGCGAGGCGCGCCATTATATCAGGATCAAGGAGCAGATCCTGGCCGAGATCGC
CGAGGGCAAGCTGCAGCCGGGAGACCGGGTATCTTCTGAAAGCGAATTGGTGGCGGCATTCGGCGTCAGCCGCATGACGG
CGAACCGGGCGCTGCGGGAGTTGATGTTCGAGGGTGTTCTCAAGCGATCCGCCGGGATCGGAACCTTCGTGTCGCCCAAG
CACCTCGACGTCGATCTGCTTCAAATCCGCAACATAGCCGACGAAATACTGGAACGGGGCCACAAGCATCAGGCGATGGT
GATCAAGGCCGGCTTGATGAAGGCGGATGCCAACGTCGCCGATGCGCTCGAACTCATCCTCGGCGCTGAGGTGATGCATT
CGCTGATCGTGCACATGGAAAACGACCAGCCCATTCAGGTCGAGGAGCGCTACGTCAATCCGCTGGTCGCGCCCGACTAT
CTGTCGGCCGACTTCCGCAGTATGACGCCGCACGAATATCTGACGGAGGTGGCTCCGATAACGGCGTTCGAACATATCGT
CCAGGCCGTCAAACCGGATCCGGCCATACGCAAATATCTCGGCCTCAGGAATGATCACCCTTGCCTGCGCGTCTTTCGAA
GAACCTGGTCAGGCGAGGCCGTCGTGACCTGCGCGCTGTTATACTATCCCGGCGCGCAATACCGGCTGGAAGCACGGTCC
ACCAAGGGTCCCTCCAGGCCCGTCGCCATTCTCGGAGAGAAACAGTGA

Upstream 100 bases:

>100_bases
TCACCGTGCAGATATGGCGCAACGACGCCGAGATTCGCTTCCGAGGCCTCGTTGCTGCGCGCGGCGCGACCATCCTCGAC
AATGGCATGGCGAGGTTGTC

Downstream 100 bases:

>100_bases
GCGCCACCAATTCTCCGTCGATCATCTTCAGCGCGGCGCCGCCGACGATCGAGGACATCGCAGCAATTGCCCGTCGTCAC
GCAAGGATCGAGGTATCCGC

Product: GntR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 255; Mature: 254

Protein sequence:

>255_residues
MTKAVKPAAGEARHYIRIKEQILAEIAEGKLQPGDRVSSESELVAAFGVSRMTANRALRELMFEGVLKRSAGIGTFVSPK
HLDVDLLQIRNIADEILERGHKHQAMVIKAGLMKADANVADALELILGAEVMHSLIVHMENDQPIQVEERYVNPLVAPDY
LSADFRSMTPHEYLTEVAPITAFEHIVQAVKPDPAIRKYLGLRNDHPCLRVFRRTWSGEAVVTCALLYYPGAQYRLEARS
TKGPSRPVAILGEKQ

Sequences:

>Translated_255_residues
MTKAVKPAAGEARHYIRIKEQILAEIAEGKLQPGDRVSSESELVAAFGVSRMTANRALRELMFEGVLKRSAGIGTFVSPK
HLDVDLLQIRNIADEILERGHKHQAMVIKAGLMKADANVADALELILGAEVMHSLIVHMENDQPIQVEERYVNPLVAPDY
LSADFRSMTPHEYLTEVAPITAFEHIVQAVKPDPAIRKYLGLRNDHPCLRVFRRTWSGEAVVTCALLYYPGAQYRLEARS
TKGPSRPVAILGEKQ
>Mature_254_residues
TKAVKPAAGEARHYIRIKEQILAEIAEGKLQPGDRVSSESELVAAFGVSRMTANRALRELMFEGVLKRSAGIGTFVSPKH
LDVDLLQIRNIADEILERGHKHQAMVIKAGLMKADANVADALELILGAEVMHSLIVHMENDQPIQVEERYVNPLVAPDYL
SADFRSMTPHEYLTEVAPITAFEHIVQAVKPDPAIRKYLGLRNDHPCLRVFRRTWSGEAVVTCALLYYPGAQYRLEARST
KGPSRPVAILGEKQ

Specific function: Repressor which binds to the hutP region in the histidine utilization (hut) operon. It blocks the expression of all the hut genes in the absence of inducer [H]

COG id: COG2188

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786950, Length=197, Percent_Identity=26.3959390862944, Blast_Score=78, Evalue=5e-16,
Organism=Escherichia coli, GI87082252, Length=146, Percent_Identity=28.0821917808219, Blast_Score=72, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010248
- InterPro:   IPR000524
- InterPro:   IPR011663
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00392 GntR; PF07702 UTRA [H]

EC number: NA

Molecular weight: Translated: 28356; Mature: 28225

Theoretical pI: Translated: 7.76; Mature: 7.76

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKAVKPAAGEARHYIRIKEQILAEIAEGKLQPGDRVSSESELVAAFGVSRMTANRALRE
CCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
LMFEGVLKRSAGIGTFVSPKHLDVDLLQIRNIADEILERGHKHQAMVIKAGLMKADANVA
HHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHCCCCHH
DALELILGAEVMHSLIVHMENDQPIQVEERYVNPLVAPDYLSADFRSMTPHEYLTEVAPI
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCHHHCCHHHCCCHHHHHHHHHHH
TAFEHIVQAVKPDPAIRKYLGLRNDHPCLRVFRRTWSGEAVVTCALLYYPGAQYRLEARS
HHHHHHHHHHCCCHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEECC
TKGPSRPVAILGEKQ
CCCCCCCEEEECCCC
>Mature Secondary Structure 
TKAVKPAAGEARHYIRIKEQILAEIAEGKLQPGDRVSSESELVAAFGVSRMTANRALRE
CCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
LMFEGVLKRSAGIGTFVSPKHLDVDLLQIRNIADEILERGHKHQAMVIKAGLMKADANVA
HHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHCCCCHH
DALELILGAEVMHSLIVHMENDQPIQVEERYVNPLVAPDYLSADFRSMTPHEYLTEVAPI
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCHHHCCHHHCCCHHHHHHHHHHH
TAFEHIVQAVKPDPAIRKYLGLRNDHPCLRVFRRTWSGEAVVTCALLYYPGAQYRLEARS
HHHHHHHHHHCCCHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEECC
TKGPSRPVAILGEKQ
CCCCCCCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]