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The map label for this gene is ygbJ [C]

Identifier: 116249281

GI number: 116249281

Start: 669694

End: 670638

Strand: Reverse

Name: ygbJ [C]

Synonym: pRL120616

Alternate gene names: 116249281

Gene position: 670638-669694 (Counterclockwise)

Preceding gene: 116249282

Following gene: 116249280

Centisome position: 77.08

GC content: 65.08

Gene sequence:

>945_bases
ATGTCACCGCTTGCTGAAAACTCAGGTCCCGACATTATCGCGGCCGTCATCGGTCTTGGTTCCATGGGGCTTGGAATGGC
CCAGTCGATGAAGCGCGCCGGGCTCGATGTCGTTGGATATGACATCACGCCGGCGGCGGTGGACCGCTTCGTCGCCGATG
GCGGACGGGGCGCGGCGACCCCCGCCGCTGCGGCAAAGGATGCCGACATCGTCGTCTCCGTGGTCGTCAACGGCGCGCAG
ACCGAGGCCGTGCTGTTCGGGCCCCAAGGCGTCGCAAATGCGATGAAGCCCGGCGCCGTCTTTATCTCGTCGGCCACCAT
GGATCCCGCCGTCGCGCGCGATCTGGCACAGCGGACGGAGGCTCTCGGCCTGCATTATCTCGACGCGCCGATTTCGGGCG
GCGCGGCCAAGGCGGCGCGTGGCGAACTGACGATCATGGCATCCGGCTCCAGACAGGCCTTCGATACGGCGCGCCCGGGT
CTCGACGCCATGGCCGCCAAGGTCTACGAGCTTGGCGACGAGGCCGGAAAAGGTGCTGCCTTCAAGATGATCAACCAGCT
TCTCGCCGGCGTGCACATCGCGGCCGCCTGTGAGGCCATAACCTTTGCCGCCAAGCAGGGCCTCGACCTCGACAAGGTCT
ATGAGGTGATCACGGCTTCGGCCGGCAATTCCTGGATGTTCGAAAACCGCGTGCCGCATGTGCTGGCCGGAGACTATACG
CCGCTCAGCAGCATCGAGATTTTCGTCAAGGATCTCGGTATCGTCCAAGACATGGCCCGCTCCGAGCGTTATCCGGTACC
GCTCGCGGCAGCCGCCCTGCAGATGTATCTGGCCGCCTCCGGGGCGGGCATGGGCCGTGACGACGATTCCTCGCTCGCGC
GGCTCTACGCCAAGCTTTCCGGCGCTGAATTGCCCAGCTCCACCAAAGAGCCGCAGAGCCTCTAA

Upstream 100 bases:

>100_bases
TGACGGTTCCGGCGAAAAGCGTCAATCGCTGAACAATAGCCCCTCCGGGATGGTACCCGGAGGGGCGCAGACCTCTCTCA
CAAGACTGGAGAAGCTCCTC

Downstream 100 bases:

>100_bases
AAGGAACGCTAGACATGCCGGTTTTCGCCGCCAACCTGACGATGATGTTCAACGAATGGTCGTTCCTCGATCGCTTCGAC
GCCGCAGCCGATGCCGGCTT

Product: putative reductase

Products: 2-methyl-3-oxopropanoate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 314; Mature: 313

Protein sequence:

>314_residues
MSPLAENSGPDIIAAVIGLGSMGLGMAQSMKRAGLDVVGYDITPAAVDRFVADGGRGAATPAAAAKDADIVVSVVVNGAQ
TEAVLFGPQGVANAMKPGAVFISSATMDPAVARDLAQRTEALGLHYLDAPISGGAAKAARGELTIMASGSRQAFDTARPG
LDAMAAKVYELGDEAGKGAAFKMINQLLAGVHIAAACEAITFAAKQGLDLDKVYEVITASAGNSWMFENRVPHVLAGDYT
PLSSIEIFVKDLGIVQDMARSERYPVPLAAAALQMYLAASGAGMGRDDDSSLARLYAKLSGAELPSSTKEPQSL

Sequences:

>Translated_314_residues
MSPLAENSGPDIIAAVIGLGSMGLGMAQSMKRAGLDVVGYDITPAAVDRFVADGGRGAATPAAAAKDADIVVSVVVNGAQ
TEAVLFGPQGVANAMKPGAVFISSATMDPAVARDLAQRTEALGLHYLDAPISGGAAKAARGELTIMASGSRQAFDTARPG
LDAMAAKVYELGDEAGKGAAFKMINQLLAGVHIAAACEAITFAAKQGLDLDKVYEVITASAGNSWMFENRVPHVLAGDYT
PLSSIEIFVKDLGIVQDMARSERYPVPLAAAALQMYLAASGAGMGRDDDSSLARLYAKLSGAELPSSTKEPQSL
>Mature_313_residues
SPLAENSGPDIIAAVIGLGSMGLGMAQSMKRAGLDVVGYDITPAAVDRFVADGGRGAATPAAAAKDADIVVSVVVNGAQT
EAVLFGPQGVANAMKPGAVFISSATMDPAVARDLAQRTEALGLHYLDAPISGGAAKAARGELTIMASGSRQAFDTARPGL
DAMAAKVYELGDEAGKGAAFKMINQLLAGVHIAAACEAITFAAKQGLDLDKVYEVITASAGNSWMFENRVPHVLAGDYTP
LSSIEIFVKDLGIVQDMARSERYPVPLAAAALQMYLAASGAGMGRDDDSSLARLYAKLSGAELPSSTKEPQSL

Specific function: Unknown

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI23308751, Length=286, Percent_Identity=31.4685314685315, Blast_Score=146, Evalue=3e-35,
Organism=Homo sapiens, GI40556376, Length=280, Percent_Identity=26.7857142857143, Blast_Score=123, Evalue=2e-28,
Organism=Escherichia coli, GI1789092, Length=299, Percent_Identity=48.494983277592, Blast_Score=259, Evalue=2e-70,
Organism=Escherichia coli, GI145693186, Length=288, Percent_Identity=33.6805555555556, Blast_Score=164, Evalue=7e-42,
Organism=Escherichia coli, GI1790315, Length=279, Percent_Identity=33.3333333333333, Blast_Score=138, Evalue=5e-34,
Organism=Escherichia coli, GI1786719, Length=278, Percent_Identity=28.4172661870504, Blast_Score=117, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17557316, Length=272, Percent_Identity=30.5147058823529, Blast_Score=121, Evalue=5e-28,
Organism=Drosophila melanogaster, GI24655230, Length=289, Percent_Identity=30.4498269896194, Blast_Score=140, Evalue=1e-33,
Organism=Drosophila melanogaster, GI19922568, Length=289, Percent_Identity=30.4498269896194, Blast_Score=140, Evalue=1e-33,
Organism=Drosophila melanogaster, GI28574115, Length=277, Percent_Identity=23.1046931407942, Blast_Score=92, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002204
- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: 1.1.1.31

Molecular weight: Translated: 32145; Mature: 32014

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: PS00895 3_HYDROXYISOBUT_DH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPLAENSGPDIIAAVIGLGSMGLGMAQSMKRAGLDVVGYDITPAAVDRFVADGGRGAAT
CCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHCCCEEEECCCCHHHHHHHHHCCCCCCCC
PAAAAKDADIVVSVVVNGAQTEAVLFGPQGVANAMKPGAVFISSATMDPAVARDLAQRTE
CCCCCCCCCEEEEEEECCCCCEEEEECCCHHHHHCCCCEEEEECCCCCHHHHHHHHHHHH
ALGLHYLDAPISGGAAKAARGELTIMASGSRQAFDTARPGLDAMAAKVYELGDEAGKGAA
HHCCEEECCCCCCCCCCCCCCCEEEEECCCCHHHHHCCCCHHHHHHHHHHHHHHCCCCHH
FKMINQLLAGVHIAAACEAITFAAKQGLDLDKVYEVITASAGNSWMFENRVPHVLAGDYT
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCEEECCCCCEEEECCCC
PLSSIEIFVKDLGIVQDMARSERYPVPLAAAALQMYLAASGAGMGRDDDSSLARLYAKLS
CHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHC
GAELPSSTKEPQSL
CCCCCCCCCCCCCC
>Mature Secondary Structure 
SPLAENSGPDIIAAVIGLGSMGLGMAQSMKRAGLDVVGYDITPAAVDRFVADGGRGAAT
CCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHCCCEEEECCCCHHHHHHHHHCCCCCCCC
PAAAAKDADIVVSVVVNGAQTEAVLFGPQGVANAMKPGAVFISSATMDPAVARDLAQRTE
CCCCCCCCCEEEEEEECCCCCEEEEECCCHHHHHCCCCEEEEECCCCCHHHHHHHHHHHH
ALGLHYLDAPISGGAAKAARGELTIMASGSRQAFDTARPGLDAMAAKVYELGDEAGKGAA
HHCCEEECCCCCCCCCCCCCCCEEEEECCCCHHHHHCCCCHHHHHHHHHHHHHHCCCCHH
FKMINQLLAGVHIAAACEAITFAAKQGLDLDKVYEVITASAGNSWMFENRVPHVLAGDYT
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCEEECCCCCEEEECCCC
PLSSIEIFVKDLGIVQDMARSERYPVPLAAAALQMYLAASGAGMGRDDDSSLARLYAKLS
CHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHC
GAELPSSTKEPQSL
CCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-hydroxy-2-methylpropanoate; NAD+

Specific reaction: 3-hydroxy-2-methylpropanoate + NAD+ = 2-methyl-3-oxopropanoate + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]