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The map label for this gene is hyi [C]

Identifier: 116249280

GI number: 116249280

Start: 668903

End: 669679

Strand: Reverse

Name: hyi [C]

Synonym: pRL120615

Alternate gene names: 116249280

Gene position: 669679-668903 (Counterclockwise)

Preceding gene: 116249281

Following gene: 116249279

Centisome position: 76.97

GC content: 61.52

Gene sequence:

>777_bases
ATGCCGGTTTTCGCCGCCAACCTGACGATGATGTTCAACGAATGGTCGTTCCTCGATCGCTTCGACGCCGCAGCCGATGC
CGGCTTTGCCGCCGTCGAATACCTTTTTCCCTATGAAGCCGCTCCGGAGGCGATCGCCGAACGGCTTGCCCGCAACAATC
TGCAGCAGGCCTTGTTCAACCTGCCGCCGGGCGACTGGGCAGCAGGCGAACGTGGCATCGCCGCTCTTCCCGGACGGTTC
GATGCGCTGAAAGCCGATGTCGAGCGGGCACTGGACTATGCGGCGGCAACGGGCGTCAGACGGTTACACCTGATGGCAGG
CATCGCCGACCGTCATGACGAAGACGCCGCCTCTTCTTATCGGCGCTCCGTCACCTATGCCGCCGGGCGGCTTGCGGAAA
AGGGCATCGATCTGCTGCTCGAGCCGATCAACGGGCGAAACATGCCGGGATATTTCCTCAACGACTTCGGCGTGGCCGAG
CGGCTGATTGCCGAATGCGGCCTGCCGAACCTGAAGCTGCAGTTCGACATCTATCACCGGCAGATCATCCGTGGCGACGT
CACTATGGCGTTGCGACGTCTGCTGCCGATCACCGGCCATATCCAGATCGCCAGCGTGCCGTCACGCAATGAGCCTGAGG
GGGAGGAACTGAACTATCCCTATCTGTTCGGCGAAATCGACCGCCTGGCTTATGACGGTTTCGTCGGCTGCGAATACATC
CCGCGCGGCCACACACTGGACGGTCTTGGCTGGTTCAAACCTTTTGCACGGAGCTAG

Upstream 100 bases:

>100_bases
ACGACGATTCCTCGCTCGCGCGGCTCTACGCCAAGCTTTCCGGCGCTGAATTGCCCAGCTCCACCAAAGAGCCGCAGAGC
CTCTAAAAGGAACGCTAGAC

Downstream 100 bases:

>100_bases
ACGATGGCTATTTTCCTCGGATCAATCGCTGACGACTATACGGGCGCCTCCGACCTTGCCAACACACTGACGAAGAACGG
CCTGCGCACGGTGCAGACGG

Product: putative hydroxypyruvate isomerase

Products: 2-hydroxy-3-oxopropanoate

Alternate protein names: ORF4 [H]

Number of amino acids: Translated: 258; Mature: 257

Protein sequence:

>258_residues
MPVFAANLTMMFNEWSFLDRFDAAADAGFAAVEYLFPYEAAPEAIAERLARNNLQQALFNLPPGDWAAGERGIAALPGRF
DALKADVERALDYAAATGVRRLHLMAGIADRHDEDAASSYRRSVTYAAGRLAEKGIDLLLEPINGRNMPGYFLNDFGVAE
RLIAECGLPNLKLQFDIYHRQIIRGDVTMALRRLLPITGHIQIASVPSRNEPEGEELNYPYLFGEIDRLAYDGFVGCEYI
PRGHTLDGLGWFKPFARS

Sequences:

>Translated_258_residues
MPVFAANLTMMFNEWSFLDRFDAAADAGFAAVEYLFPYEAAPEAIAERLARNNLQQALFNLPPGDWAAGERGIAALPGRF
DALKADVERALDYAAATGVRRLHLMAGIADRHDEDAASSYRRSVTYAAGRLAEKGIDLLLEPINGRNMPGYFLNDFGVAE
RLIAECGLPNLKLQFDIYHRQIIRGDVTMALRRLLPITGHIQIASVPSRNEPEGEELNYPYLFGEIDRLAYDGFVGCEYI
PRGHTLDGLGWFKPFARS
>Mature_257_residues
PVFAANLTMMFNEWSFLDRFDAAADAGFAAVEYLFPYEAAPEAIAERLARNNLQQALFNLPPGDWAAGERGIAALPGRFD
ALKADVERALDYAAATGVRRLHLMAGIADRHDEDAASSYRRSVTYAAGRLAEKGIDLLLEPINGRNMPGYFLNDFGVAER
LIAECGLPNLKLQFDIYHRQIIRGDVTMALRRLLPITGHIQIASVPSRNEPEGEELNYPYLFGEIDRLAYDGFVGCEYIP
RGHTLDGLGWFKPFARS

Specific function: Catalyzes The Reversible Isomerization Between Hydroxypyruvate And 2-Hydroxy-3-Oxopropanoate (Also Termed Tartronate Semialdehyde). [C]

COG id: COG3622

COG function: function code G; Hydroxypyruvate isomerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hyi family [H]

Homologues:

Organism=Homo sapiens, GI300068962, Length=261, Percent_Identity=44.8275862068966, Blast_Score=200, Evalue=1e-51,
Organism=Homo sapiens, GI300068964, Length=244, Percent_Identity=43.8524590163934, Blast_Score=173, Evalue=1e-43,
Organism=Escherichia coli, GI1786718, Length=257, Percent_Identity=43.1906614785992, Blast_Score=212, Evalue=2e-56,
Organism=Escherichia coli, GI1789095, Length=254, Percent_Identity=42.9133858267717, Blast_Score=204, Evalue=5e-54,
Organism=Caenorhabditis elegans, GI17552022, Length=239, Percent_Identity=37.2384937238494, Blast_Score=163, Evalue=9e-41,
Organism=Drosophila melanogaster, GI17530883, Length=253, Percent_Identity=32.0158102766798, Blast_Score=141, Evalue=6e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013022
- InterPro:   IPR012307 [H]

Pfam domain/function: PF01261 AP_endonuc_2 [H]

EC number: 5.3.1.22

Molecular weight: Translated: 28700; Mature: 28569

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVFAANLTMMFNEWSFLDRFDAAADAGFAAVEYLFPYEAAPEAIAERLARNNLQQALFN
CCEEECHHEEEECCHHHHHHHHHHHCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHC
LPPGDWAAGERGIAALPGRFDALKADVERALDYAAATGVRRLHLMAGIADRHDEDAASSY
CCCCCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
RRSVTYAAGRLAEKGIDLLLEPINGRNMPGYFLNDFGVAERLIAECGLPNLKLQFDIYHR
HHHHHHHHHHHHHHCHHEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEHHHH
QIIRGDVTMALRRLLPITGHIQIASVPSRNEPEGEELNYPYLFGEIDRLAYDGFVGCEYI
HHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEC
PRGHTLDGLGWFKPFARS
CCCCCCCCCHHCCHHHCC
>Mature Secondary Structure 
PVFAANLTMMFNEWSFLDRFDAAADAGFAAVEYLFPYEAAPEAIAERLARNNLQQALFN
CEEECHHEEEECCHHHHHHHHHHHCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHC
LPPGDWAAGERGIAALPGRFDALKADVERALDYAAATGVRRLHLMAGIADRHDEDAASSY
CCCCCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
RRSVTYAAGRLAEKGIDLLLEPINGRNMPGYFLNDFGVAERLIAECGLPNLKLQFDIYHR
HHHHHHHHHHHHHHCHHEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEHHHH
QIIRGDVTMALRRLLPITGHIQIASVPSRNEPEGEELNYPYLFGEIDRLAYDGFVGCEYI
HHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEC
PRGHTLDGLGWFKPFARS
CCCCCCCCCHHCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 10 {hydroxypyruvate}} [C]

Substrates: hydroxypyruvate

Specific reaction: hydroxypyruvate = 2-hydroxy-3-oxopropanoate; Other Name(s): hydroxypyruvate ketol-isomerase

General reaction: Intramolecular oxidoreduction; Isomerization [C]

Inhibitor: Cu2+; Fe2+; Hydroxy l amine; Iodoacetate; NaCN [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7851418 [H]