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The map label for this gene is braD [H]

Identifier: 116249109

GI number: 116249109

Start: 478023

End: 478916

Strand: Reverse

Name: braD [H]

Synonym: pRL120444

Alternate gene names: 116249109

Gene position: 478916-478023 (Counterclockwise)

Preceding gene: 116249110

Following gene: 116249108

Centisome position: 55.05

GC content: 62.08

Gene sequence:

>894_bases
ATGCTCGACATTCTGTTTATCGGCCTCAGTCTCGGCTCGATCCTTCTTTTGGTGGCGCTCGGCCTTGCCATCACCTACGG
CGCTATGGGCGTCATCAACATGGCTCATGGAGAGATGGTCATGATCGGCGCCTATGTCGCCGTCCTCTCCGGCATCTGGC
TGAAGGCAAGCCTTCTGCTTGCGATCCCGCTGGCCTTCGTCGTGACGGCCCTGCTCGGTCTGCTGATCGAGCGGGTGGTT
GTGCGGCGGCTTTATGGCCGTCTGCTGGACACGCTTCTCGCCACCTGGGGGATCGCCATCCTCCTGCAGCAGGCCGTGCG
GCTCGAATTCGGCCTGTCCTTTTTCGGCATTCATGTCGAAGGTCTTGGGGCCGGCCTGCAGAACGTCCCGGTGCCTGCCT
ACCTGCAGGGCACGTTCAGGCTTGCCGGCGCCGACATCAATGCCTACCGCACCTTTATCATCGCCGTTACCGCCGCGCTG
ACACTGGCGACGTGGTTCATTCTCTACCGGACGGCGGCCGGCATGCAGGTTCGCGCCATCATCCGGAACCCGAAAATGGC
GGCAGCCTGCGGCATCGACGTGAAACGCGTCAACGCCATGACATTTGCGTTCGGTTCCGGCCTTGCCGGTGTTGCCGGCG
TCATGATGTCCGGCTTCAAGACGGTCTTTCCCGATATGGGCACGACAATGGTCGTCGACGGCTTCATGGTCGTCGTCACC
GGCGGGGTCGGTAGCCTTTTCGGCACCATCCTGTCGTCAGGGCTGCTCGGCGAAATCAATGCGCTGGTCGCCATCGGGAC
GAACGACATCCTGGCACGTGCCGTCGTGTTCGGGGTCGTCATTCTCGTCATTCTCGTCAAGCCCAGCGGGCTGTTCTCGT
TCAAGGGGCGCTGA

Upstream 100 bases:

>100_bases
AAGGCGGGCCTCCACTCTTCTTCCCCAAGCTGCCATCCTGCCGTCAGGATCGGCAAGGCGCAGCAGAGTTCGGCCCACCC
TTGTCACGAACGGTGGCGTT

Downstream 100 bases:

>100_bases
CATGAGCATCGAACGGAAATTCCAGGTCGCGGCCTATGTGCTCTTCATCGCGGTGATTTTTGCAGCGCCCGTTTTTGGCG
ACGAATTCTGGCTGAATCGC

Product: putative permease component of ABC transporter

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MLDILFIGLSLGSILLLVALGLAITYGAMGVINMAHGEMVMIGAYVAVLSGIWLKASLLLAIPLAFVVTALLGLLIERVV
VRRLYGRLLDTLLATWGIAILLQQAVRLEFGLSFFGIHVEGLGAGLQNVPVPAYLQGTFRLAGADINAYRTFIIAVTAAL
TLATWFILYRTAAGMQVRAIIRNPKMAAACGIDVKRVNAMTFAFGSGLAGVAGVMMSGFKTVFPDMGTTMVVDGFMVVVT
GGVGSLFGTILSSGLLGEINALVAIGTNDILARAVVFGVVILVILVKPSGLFSFKGR

Sequences:

>Translated_297_residues
MLDILFIGLSLGSILLLVALGLAITYGAMGVINMAHGEMVMIGAYVAVLSGIWLKASLLLAIPLAFVVTALLGLLIERVV
VRRLYGRLLDTLLATWGIAILLQQAVRLEFGLSFFGIHVEGLGAGLQNVPVPAYLQGTFRLAGADINAYRTFIIAVTAAL
TLATWFILYRTAAGMQVRAIIRNPKMAAACGIDVKRVNAMTFAFGSGLAGVAGVMMSGFKTVFPDMGTTMVVDGFMVVVT
GGVGSLFGTILSSGLLGEINALVAIGTNDILARAVVFGVVILVILVKPSGLFSFKGR
>Mature_297_residues
MLDILFIGLSLGSILLLVALGLAITYGAMGVINMAHGEMVMIGAYVAVLSGIWLKASLLLAIPLAFVVTALLGLLIERVV
VRRLYGRLLDTLLATWGIAILLQQAVRLEFGLSFFGIHVEGLGAGLQNVPVPAYLQGTFRLAGADINAYRTFIIAVTAAL
TLATWFILYRTAAGMQVRAIIRNPKMAAACGIDVKRVNAMTFAFGSGLAGVAGVMMSGFKTVFPDMGTTMVVDGFMVVVT
GGVGSLFGTILSSGLLGEINALVAIGTNDILARAVVFGVVILVILVKPSGLFSFKGR

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789866, Length=310, Percent_Identity=32.5806451612903, Blast_Score=91, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851 [H]

Pfam domain/function: PF02653 BPD_transp_2 [H]

EC number: NA

Molecular weight: Translated: 31139; Mature: 31139

Theoretical pI: Translated: 10.27; Mature: 10.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDILFIGLSLGSILLLVALGLAITYGAMGVINMAHGEMVMIGAYVAVLSGIWLKASLLL
CCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHH
AIPLAFVVTALLGLLIERVVVRRLYGRLLDTLLATWGIAILLQQAVRLEFGLSFFGIHVE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEE
GLGAGLQNVPVPAYLQGTFRLAGADINAYRTFIIAVTAALTLATWFILYRTAAGMQVRAI
ECCCCCCCCCCCCEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEE
IRNPKMAAACGIDVKRVNAMTFAFGSGLAGVAGVMMSGFKTVFPDMGTTMVVDGFMVVVT
CCCCCHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCHHHHCCEEEEEE
GGVGSLFGTILSSGLLGEINALVAIGTNDILARAVVFGVVILVILVKPSGLFSFKGR
CCHHHHHHHHHCCCCHHCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MLDILFIGLSLGSILLLVALGLAITYGAMGVINMAHGEMVMIGAYVAVLSGIWLKASLLL
CCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHH
AIPLAFVVTALLGLLIERVVVRRLYGRLLDTLLATWGIAILLQQAVRLEFGLSFFGIHVE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEE
GLGAGLQNVPVPAYLQGTFRLAGADINAYRTFIIAVTAALTLATWFILYRTAAGMQVRAI
ECCCCCCCCCCCCEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEE
IRNPKMAAACGIDVKRVNAMTFAFGSGLAGVAGVMMSGFKTVFPDMGTTMVVDGFMVVVT
CCCCCHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCHHHHCCEEEEEE
GGVGSLFGTILSSGLLGEINALVAIGTNDILARAVVFGVVILVILVKPSGLFSFKGR
CCHHHHHHHHHCCCCHHCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043; 2509433 [H]