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The map label for this gene is livM [H]

Identifier: 116249108

GI number: 116249108

Start: 476882

End: 478021

Strand: Reverse

Name: livM [H]

Synonym: pRL120443

Alternate gene names: 116249108

Gene position: 478021-476882 (Counterclockwise)

Preceding gene: 116249109

Following gene: 116249107

Centisome position: 54.94

GC content: 59.65

Gene sequence:

>1140_bases
ATGAGCATCGAACGGAAATTCCAGGTCGCGGCCTATGTGCTCTTCATCGCGGTGATTTTTGCAGCGCCCGTTTTTGGCGA
CGAATTCTGGCTGAATCGCATTTCGAAATACCTGGTCTACGGCATGCTGGGCGTCGCCATCGCGCTCACATGGGGATATG
CAGGTATTCTCAATCTCGGCCAGGGACTGTTTTTCGGCCTCGGCGCATACATGCTGGCCATGTCGCTGAAGCTGTCGAGC
CTGACCAGTCTCCAACAGGGCTCCGATAAGCCCGTTCCTGATTTCATGCTCTGGAATGCCGAGCCCGGCGCGCCGACGGA
GCTTTGCTGCATCAACAAGGGCTCCTTCCTCTGGCTTCCGTTTCAAAGCCAGTCGGTGGGGCTGGTGCTCGGCATCGTGC
TTCCGGTGGTAATTGCCGGAGCACTTGGCATGCTGGTTTTCCGCAAGCGCATTTCTGGCGTTTTCGTCTCGATCATCACG
CTGGCGCTCGTTCTGCTTGTCCGCCTCGTCGTGGTCGATGCGCAACCGGTGACCAACGGCTTCAACGGCCTGACGGATCT
TGGCTGGCTGACGATCGGCGGCATCGAATTCGATCCCTACAGCCGGGCGACCTATTATCTGTGTGCCACATGCCTGTCGC
TGACGCTCATCGGTGCCCGTCTCATTGTTGAAACCCGCGCCGGCATGATCCTGCAGGCCATCCGCGACGATCATGTGCGC
GCCCGCTATCTCGGCTTCGACGTCTCGCTCTACCAGGTGTTTTTCTTCGTGATTTCGGCGGGGATCGCCGGCGTTGCCGG
CATGCTCTACGTGGTCGTCGCAGAGTTCGCCTCTCCGACGTTCATGGACCTGTCCTTTTCCGTGACGATGGTGGTCTGGG
CTGCCGTCGGCGGCCGCTCCTCCCTGCTCGGCGCCTGCATCGGCGCGATCCTGATCAACATGATCGAGGCGGAGGTCAGC
GAGACCGAAGCGCTGGTCGAGGCATGGAAGGCAATCATCGGTCTCATCTTCGTGCTGGTCGTTCTGTTCATGCCGCGTGG
CCTTGGCGGCGTTGCACATGATGTGCTGCGGCAGTTTATCCGCCGGCTCAAACCAGGCGCTGCATCTTCATCGTTGCAGC
AACAAAGCGAGGCATTGTGA

Upstream 100 bases:

>100_bases
GGGACGAACGACATCCTGGCACGTGCCGTCGTGTTCGGGGTCGTCATTCTCGTCATTCTCGTCAAGCCCAGCGGGCTGTT
CTCGTTCAAGGGGCGCTGAC

Downstream 100 bases:

>100_bases
TATGAGTGCGATCGCATTGACCATCGACGGTCTCAGCGTCGATTTTGGCGGCTTCCGCGCCGTCAACAATGTCAGCATCA
CCGTCTTCGACGGCGAGCTG

Product: putative permease component of ABC transporter

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: LIV-I protein M [H]

Number of amino acids: Translated: 379; Mature: 378

Protein sequence:

>379_residues
MSIERKFQVAAYVLFIAVIFAAPVFGDEFWLNRISKYLVYGMLGVAIALTWGYAGILNLGQGLFFGLGAYMLAMSLKLSS
LTSLQQGSDKPVPDFMLWNAEPGAPTELCCINKGSFLWLPFQSQSVGLVLGIVLPVVIAGALGMLVFRKRISGVFVSIIT
LALVLLVRLVVVDAQPVTNGFNGLTDLGWLTIGGIEFDPYSRATYYLCATCLSLTLIGARLIVETRAGMILQAIRDDHVR
ARYLGFDVSLYQVFFFVISAGIAGVAGMLYVVVAEFASPTFMDLSFSVTMVVWAAVGGRSSLLGACIGAILINMIEAEVS
ETEALVEAWKAIIGLIFVLVVLFMPRGLGGVAHDVLRQFIRRLKPGAASSSLQQQSEAL

Sequences:

>Translated_379_residues
MSIERKFQVAAYVLFIAVIFAAPVFGDEFWLNRISKYLVYGMLGVAIALTWGYAGILNLGQGLFFGLGAYMLAMSLKLSS
LTSLQQGSDKPVPDFMLWNAEPGAPTELCCINKGSFLWLPFQSQSVGLVLGIVLPVVIAGALGMLVFRKRISGVFVSIIT
LALVLLVRLVVVDAQPVTNGFNGLTDLGWLTIGGIEFDPYSRATYYLCATCLSLTLIGARLIVETRAGMILQAIRDDHVR
ARYLGFDVSLYQVFFFVISAGIAGVAGMLYVVVAEFASPTFMDLSFSVTMVVWAAVGGRSSLLGACIGAILINMIEAEVS
ETEALVEAWKAIIGLIFVLVVLFMPRGLGGVAHDVLRQFIRRLKPGAASSSLQQQSEAL
>Mature_378_residues
SIERKFQVAAYVLFIAVIFAAPVFGDEFWLNRISKYLVYGMLGVAIALTWGYAGILNLGQGLFFGLGAYMLAMSLKLSSL
TSLQQGSDKPVPDFMLWNAEPGAPTELCCINKGSFLWLPFQSQSVGLVLGIVLPVVIAGALGMLVFRKRISGVFVSIITL
ALVLLVRLVVVDAQPVTNGFNGLTDLGWLTIGGIEFDPYSRATYYLCATCLSLTLIGARLIVETRAGMILQAIRDDHVRA
RYLGFDVSLYQVFFFVISAGIAGVAGMLYVVVAEFASPTFMDLSFSVTMVVWAAVGGRSSLLGACIGAILINMIEAEVSE
TEALVEAWKAIIGLIFVLVVLFMPRGLGGVAHDVLRQFIRRLKPGAASSSLQQQSEAL

Specific function: Part of the binding-protein-dependent transport system for branched-chain amino acids. Probably responsible for the translocation of the substrates across the membrane [H]

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=359, Percent_Identity=23.3983286908078, Blast_Score=69, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 40905; Mature: 40774

Theoretical pI: Translated: 7.24; Mature: 7.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIERKFQVAAYVLFIAVIFAAPVFGDEFWLNRISKYLVYGMLGVAIALTWGYAGILNLG
CCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QGLFFGLGAYMLAMSLKLSSLTSLQQGSDKPVPDFMLWNAEPGAPTELCCINKGSFLWLP
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCEEEEECCCCEEEEE
FQSQSVGLVLGIVLPVVIAGALGMLVFRKRISGVFVSIITLALVLLVRLVVVDAQPVTNG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCC
FNGLTDLGWLTIGGIEFDPYSRATYYLCATCLSLTLIGARLIVETRAGMILQAIRDDHVR
CCCHHHCCCEEECCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
ARYLGFDVSLYQVFFFVISAGIAGVAGMLYVVVAEFASPTFMDLSFSVTMVVWAAVGGRS
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHCCCH
SLLGACIGAILINMIEAEVSETEALVEAWKAIIGLIFVLVVLFMPRGLGGVAHDVLRQFI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
RRLKPGAASSSLQQQSEAL
HHCCCCCCHHHHHHHHCCC
>Mature Secondary Structure 
SIERKFQVAAYVLFIAVIFAAPVFGDEFWLNRISKYLVYGMLGVAIALTWGYAGILNLG
CCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QGLFFGLGAYMLAMSLKLSSLTSLQQGSDKPVPDFMLWNAEPGAPTELCCINKGSFLWLP
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCEEEEECCCCEEEEE
FQSQSVGLVLGIVLPVVIAGALGMLVFRKRISGVFVSIITLALVLLVRLVVVDAQPVTNG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCC
FNGLTDLGWLTIGGIEFDPYSRATYYLCATCLSLTLIGARLIVETRAGMILQAIRDDHVR
CCCHHHCCCEEECCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
ARYLGFDVSLYQVFFFVISAGIAGVAGMLYVVVAEFASPTFMDLSFSVTMVVWAAVGGRS
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHCCCH
SLLGACIGAILINMIEAEVSETEALVEAWKAIIGLIFVLVVLFMPRGLGGVAHDVLRQFI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
RRLKPGAASSSLQQQSEAL
HHCCCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2195019; 8041620; 9278503 [H]