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The map label for this gene is amiC [H]

Identifier: 116249110

GI number: 116249110

Start: 479067

End: 480317

Strand: Reverse

Name: amiC [H]

Synonym: pRL120445

Alternate gene names: 116249110

Gene position: 480317-479067 (Counterclockwise)

Preceding gene: 116249111

Following gene: 116249109

Centisome position: 55.21

GC content: 60.43

Gene sequence:

>1251_bases
ATGGTGTTCAACAGACGTGAATTTCTGAAAACCGCAGCGACCGCTTCCGCCGCACTCGCCCTGCCGGCTCTGGGCAGCCG
CGCCTTCGCAGCCGATGCCATCAAGGTCGGCGCGCTTTACTCGCAGACGGGCGGGCTTTCGGTTGCCGAGAAGCTTCTGG
CGAACGGCGTCATGATGGCCGTGGCCGAAATCAACGCGACCGGCGGTGTACTCGGTCGCCCCGTCGAGGTGGTCGTCGAA
GACGGTGCTTCCGATCCGAAAACCTTCAGCGAGAAGGCTTCGAAACTGATCCTCAAGGACAAGATTTCGACCGTGTTCGG
CTGCCACACATCAGCAAGCCGCAAGGCCGTGCTGCCGATCTTCGAGCGACGTGGCGCCATGCTGTTCTACCAGACGCATT
ACGAAGGCTTCGAGTGCTCGCGCAACGTCGTCTATTCCGGCGCCGTTCCGAACCAGCAGCTCTCCAACTACATTCCCTGG
ATCGTCGAGAAACTGGGCAAGAAGAAATTCTTCATCGTCGGCTCGAACTATGTCTATCCGCGCGAAATGGCTAAGGTTTC
CAAGAAGCTGATCGAGGCAGCCGGCGCCGAGTGGGTGGCGGACGAATATCTGGAACTTGGCCATTCGGAATGGGCCGTCA
TGGTCAGCAAGATCAAGGAGTCGGGCGCCGACGTGGTCCTGTCCAACGTGGTCGGCGACTCCATCATCGCCTTCTACCGC
GAGTTCAAGAACCAGGGGCTCTCGCAGGAGGTTCTGCCGATCTGCGCGACGGTCACCTCCGAAATCGAGATCGCGGCGAT
GGGCGCCGAATATGCCGCCGGAAGCTACACCTCCTTCCCCTATTTTATGTCGATCGACACGCCGGCGAACAAGAGCTTCA
TCGATCGCTTCAGGACCTTCGTCAACGATCCGAAAGCCGTCACCTACCATTCTCTCGAAGCCGCCTATTTTCAGGTCTTC
CTGTGGAAGCAGGCCGTCGAAAAATCCGGCGACCTGTCCGCGGACGCGATCAGGGCCGGAATTCGCAGCCAAACCTACGA
AGCGCCCGGCGGCAAGGTGACCACCGACCCCGACAACCTGCATTGCTGGCTGACGCCGCGCATCGGGCAATGGCAGGCGG
ACGGCCAGAGCAAGGTGGTGAACGCCTACCCGGCGCCGATCAAGCCTCTGCCCTATTCGGCCTATGGGGAGACGGAGAGC
AATCTGTTCTGCACCAGCAACGGTCTCGACGCCGCAAAGCTCAAGGGCTGA

Upstream 100 bases:

>100_bases
TGACAGTGACGTCAGTCCTCTATCCATTTTTCGGCATGAAAGTCTCGCCGTTTGGCGGGCTCTGCCGCCGTCCGCGCCAA
TCAAAAAAAGGGGAACCAAG

Downstream 100 bases:

>100_bases
CCCCTCCCGCGTCGTCCCGCCATCCATGGGTGGCGGGATCATGTCTCGTTAAGGCGGGCCTCCACTCTTCTTCCCCAAGC
TGCCATCCTGCCGTCAGGAT

Product: putative substrate-binding component of ABC transporter

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 416; Mature: 416

Protein sequence:

>416_residues
MVFNRREFLKTAATASAALALPALGSRAFAADAIKVGALYSQTGGLSVAEKLLANGVMMAVAEINATGGVLGRPVEVVVE
DGASDPKTFSEKASKLILKDKISTVFGCHTSASRKAVLPIFERRGAMLFYQTHYEGFECSRNVVYSGAVPNQQLSNYIPW
IVEKLGKKKFFIVGSNYVYPREMAKVSKKLIEAAGAEWVADEYLELGHSEWAVMVSKIKESGADVVLSNVVGDSIIAFYR
EFKNQGLSQEVLPICATVTSEIEIAAMGAEYAAGSYTSFPYFMSIDTPANKSFIDRFRTFVNDPKAVTYHSLEAAYFQVF
LWKQAVEKSGDLSADAIRAGIRSQTYEAPGGKVTTDPDNLHCWLTPRIGQWQADGQSKVVNAYPAPIKPLPYSAYGETES
NLFCTSNGLDAAKLKG

Sequences:

>Translated_416_residues
MVFNRREFLKTAATASAALALPALGSRAFAADAIKVGALYSQTGGLSVAEKLLANGVMMAVAEINATGGVLGRPVEVVVE
DGASDPKTFSEKASKLILKDKISTVFGCHTSASRKAVLPIFERRGAMLFYQTHYEGFECSRNVVYSGAVPNQQLSNYIPW
IVEKLGKKKFFIVGSNYVYPREMAKVSKKLIEAAGAEWVADEYLELGHSEWAVMVSKIKESGADVVLSNVVGDSIIAFYR
EFKNQGLSQEVLPICATVTSEIEIAAMGAEYAAGSYTSFPYFMSIDTPANKSFIDRFRTFVNDPKAVTYHSLEAAYFQVF
LWKQAVEKSGDLSADAIRAGIRSQTYEAPGGKVTTDPDNLHCWLTPRIGQWQADGQSKVVNAYPAPIKPLPYSAYGETES
NLFCTSNGLDAAKLKG
>Mature_416_residues
MVFNRREFLKTAATASAALALPALGSRAFAADAIKVGALYSQTGGLSVAEKLLANGVMMAVAEINATGGVLGRPVEVVVE
DGASDPKTFSEKASKLILKDKISTVFGCHTSASRKAVLPIFERRGAMLFYQTHYEGFECSRNVVYSGAVPNQQLSNYIPW
IVEKLGKKKFFIVGSNYVYPREMAKVSKKLIEAAGAEWVADEYLELGHSEWAVMVSKIKESGADVVLSNVVGDSIIAFYR
EFKNQGLSQEVLPICATVTSEIEIAAMGAEYAAGSYTSFPYFMSIDTPANKSFIDRFRTFVNDPKAVTYHSLEAAYFQVF
LWKQAVEKSGDLSADAIRAGIRSQTYEAPGGKVTTDPDNLHCWLTPRIGQWQADGQSKVVNAYPAPIKPLPYSAYGETES
NLFCTSNGLDAAKLKG

Specific function: Negatively regulates the expression of the aliphatic amidase operon. AmiC functions by inhibiting the action of AmiR at the protein level. It exhibits protein kinase activity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789867, Length=379, Percent_Identity=22.6912928759894, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI48994941, Length=313, Percent_Identity=23.0031948881789, Blast_Score=70, Evalue=3e-13,

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000709 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 45212; Mature: 45212

Theoretical pI: Translated: 7.45; Mature: 7.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVFNRREFLKTAATASAALALPALGSRAFAADAIKVGALYSQTGGLSVAEKLLANGVMMA
CCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCEEE
VAEINATGGVLGRPVEVVVEDGASDPKTFSEKASKLILKDKISTVFGCHTSASRKAVLPI
EEEECCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECHH
FERRGAMLFYQTHYEGFECSRNVVYSGAVPNQQLSNYIPWIVEKLGKKKFFIVGSNYVYP
HHCCCCEEEEEECCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCH
REMAKVSKKLIEAAGAEWVADEYLELGHSEWAVMVSKIKESGADVVLSNVVGDSIIAFYR
HHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHH
EFKNQGLSQEVLPICATVTSEIEIAAMGAEYAAGSYTSFPYFMSIDTPANKSFIDRFRTF
HHHHCCCCHHHHHHHHHHHCCEEEEEECCHHCCCCCCCCCEEEEECCCCCHHHHHHHHHH
VNDPKAVTYHSLEAAYFQVFLWKQAVEKSGDLSADAIRAGIRSQTYEAPGGKVTTDPDNL
HCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCEECCCCEEECCCCCE
HCWLTPRIGQWQADGQSKVVNAYPAPIKPLPYSAYGETESNLFCTSNGLDAAKLKG
EEEECCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCEEEECCCCCHHCCCC
>Mature Secondary Structure
MVFNRREFLKTAATASAALALPALGSRAFAADAIKVGALYSQTGGLSVAEKLLANGVMMA
CCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCEEE
VAEINATGGVLGRPVEVVVEDGASDPKTFSEKASKLILKDKISTVFGCHTSASRKAVLPI
EEEECCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECHH
FERRGAMLFYQTHYEGFECSRNVVYSGAVPNQQLSNYIPWIVEKLGKKKFFIVGSNYVYP
HHCCCCEEEEEECCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCH
REMAKVSKKLIEAAGAEWVADEYLELGHSEWAVMVSKIKESGADVVLSNVVGDSIIAFYR
HHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHH
EFKNQGLSQEVLPICATVTSEIEIAAMGAEYAAGSYTSFPYFMSIDTPANKSFIDRFRTF
HHHHCCCCHHHHHHHHHHHCCEEEEEECCHHCCCCCCCCCEEEEECCCCCHHHHHHHHHH
VNDPKAVTYHSLEAAYFQVFLWKQAVEKSGDLSADAIRAGIRSQTYEAPGGKVTTDPDNL
HCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCEECCCCEEECCCCCE
HCWLTPRIGQWQADGQSKVVNAYPAPIKPLPYSAYGETESNLFCTSNGLDAAKLKG
EEEECCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCEEEECCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1907262; 10984043; 1762155; 7813419; 10508151; 10708652 [H]