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The map label for this gene is dadX

Identifier: 116249082

GI number: 116249082

Start: 450216

End: 451349

Strand: Direct

Name: dadX

Synonym: pRL120416

Alternate gene names: 116249082

Gene position: 450216-451349 (Clockwise)

Preceding gene: 116249080

Following gene: 116249083

Centisome position: 51.75

GC content: 63.4

Gene sequence:

>1134_bases
ATGGATAGCGATATTTTGGTTTCCCGCACACGCACCGCTACGATCGCGCAGGGAGCCACGGGCTATCTGATGATCGATCT
TGCCGCCCTCGGCCGTAACTATCGCAAGCTCGTATCGATGCTGGCACCGGTCCGCGCCGGCGCCGTCGTCAAGGCCGATG
CCTATGGTCTCGGCGCCGAGCGGGTCGCGAGAACGCTTTACAGTGAAGGTTGCAGGCATTTCTTCGTCGCCCAATTTGTC
GAGGCCGTGAGGCTCAGACCGGCCCTTGCGCACGACGCCCAGATTTTCGTGCTGAACGGCTTGCAGCCGGGCAACGAGAT
CGCCTGCGCCGAGATGGGCATTGTCCCGGTTCTCAATTCGCTGGCGCAGTGGCGGCAGTGGTCGGCGGCTGCGCGCATTC
TGAAGCGCTGCCTGCCAGCCGTCCTCCAATTCGACACCGGGATGTCGCGGCTCGGTTTTCCCCGGGAAGAGCGAAGAGAG
CTGGCGGCGGCTCTTCGCGATGGCAGCAATGTCGAAATCCTGTTCATCATGAGCCATCTGGCTTCGGCCGATGACATGGG
CAGCGAGCAGAACGGCGAACAGTTTGCCGAGATGTCCCGCATCGCCGACGAATTTCCCGGCTTCGACATTTCCTTCGCCA
ATTCCGGCGGCGTTTTCCTGGGCGAGGCCTATTACGGCGTACTCGCCCGCCCCGGCATCGCGCTTTATGGCGGCGCTCCG
AACGCCGGCGAGAAGAACCCGATGGAGCCGGTCGTCAGCCTCAATGTCGCCGTTGTGCAGACGCGCACCGTACCGGCGGG
TGCGAAGGTCGGCTATGGCGGCGCGCATGTCACGCAACGGGAAACGCGTCTCGCCACCATTGCCGCCGGCTATGCTGATG
GCCTGCCGCGATGCCTCAGCGACCGCGGCGCCGTCTATTTCAAGGGCGTCCGCCTGCCGATCGTCGGCCGCGTGTCGATG
GACAGCACGACCGTCGACATCACAGCACTGCCCGAAGGGGCGCTGACCTTCGGCAGTCTCGTCGAAGTGCTCGGCCGCCA
TCAGACGCTTGAGGACATCGCGCGCGATGCCGGCACCATTTCTTACGAAATCCTAACTGGCCTGGGCGATCGTTACGACA
GGCAATATCGCTAA

Upstream 100 bases:

>100_bases
TTATGCGCATAATGGGCAAATCAAGCAATAATCGGCTACTGAATGATAGGCAATTTCGCTCATTATTTCATAAACTCCCT
GAGATCAAAAAGGGGTGGAC

Downstream 100 bases:

>100_bases
GAGCCGGCGGCTCCATCATTGGGGACATCATGAAGGTCATCGTTCTAGGAGCCGGCATCGTCGGCGTTACATCCGCTTAT
CAACTGGCCAAGGCCGGTCA

Product: putative alanine racemase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 377; Mature: 377

Protein sequence:

>377_residues
MDSDILVSRTRTATIAQGATGYLMIDLAALGRNYRKLVSMLAPVRAGAVVKADAYGLGAERVARTLYSEGCRHFFVAQFV
EAVRLRPALAHDAQIFVLNGLQPGNEIACAEMGIVPVLNSLAQWRQWSAAARILKRCLPAVLQFDTGMSRLGFPREERRE
LAAALRDGSNVEILFIMSHLASADDMGSEQNGEQFAEMSRIADEFPGFDISFANSGGVFLGEAYYGVLARPGIALYGGAP
NAGEKNPMEPVVSLNVAVVQTRTVPAGAKVGYGGAHVTQRETRLATIAAGYADGLPRCLSDRGAVYFKGVRLPIVGRVSM
DSTTVDITALPEGALTFGSLVEVLGRHQTLEDIARDAGTISYEILTGLGDRYDRQYR

Sequences:

>Translated_377_residues
MDSDILVSRTRTATIAQGATGYLMIDLAALGRNYRKLVSMLAPVRAGAVVKADAYGLGAERVARTLYSEGCRHFFVAQFV
EAVRLRPALAHDAQIFVLNGLQPGNEIACAEMGIVPVLNSLAQWRQWSAAARILKRCLPAVLQFDTGMSRLGFPREERRE
LAAALRDGSNVEILFIMSHLASADDMGSEQNGEQFAEMSRIADEFPGFDISFANSGGVFLGEAYYGVLARPGIALYGGAP
NAGEKNPMEPVVSLNVAVVQTRTVPAGAKVGYGGAHVTQRETRLATIAAGYADGLPRCLSDRGAVYFKGVRLPIVGRVSM
DSTTVDITALPEGALTFGSLVEVLGRHQTLEDIARDAGTISYEILTGLGDRYDRQYR
>Mature_377_residues
MDSDILVSRTRTATIAQGATGYLMIDLAALGRNYRKLVSMLAPVRAGAVVKADAYGLGAERVARTLYSEGCRHFFVAQFV
EAVRLRPALAHDAQIFVLNGLQPGNEIACAEMGIVPVLNSLAQWRQWSAAARILKRCLPAVLQFDTGMSRLGFPREERRE
LAAALRDGSNVEILFIMSHLASADDMGSEQNGEQFAEMSRIADEFPGFDISFANSGGVFLGEAYYGVLARPGIALYGGAP
NAGEKNPMEPVVSLNVAVVQTRTVPAGAKVGYGGAHVTQRETRLATIAAGYADGLPRCLSDRGAVYFKGVRLPIVGRVSM
DSTTVDITALPEGALTFGSLVEVLGRHQTLEDIARDAGTISYEILTGLGDRYDRQYR

Specific function: Isomerizes L-alanine to D-alanine which is then oxidized to pyruvate by DadA

COG id: COG0787

COG function: function code M; Alanine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alanine racemase family

Homologues:

Organism=Escherichia coli, GI1787439, Length=355, Percent_Identity=31.5492957746479, Blast_Score=145, Evalue=3e-36,
Organism=Escherichia coli, GI1790487, Length=369, Percent_Identity=33.6043360433604, Blast_Score=140, Evalue=1e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ALR2_RHIL3 (Q9RAE7)

Other databases:

- EMBL:   AJ249196
- EMBL:   AM236086
- RefSeq:   YP_764923.1
- ProteinModelPortal:   Q9RAE7
- SMR:   Q9RAE7
- STRING:   Q9RAE7
- GeneID:   4398234
- GenomeReviews:   AM236086_GR
- KEGG:   rle:pRL120416
- NMPDR:   fig|216596.1.peg.6120
- eggNOG:   COG0787
- HOGENOM:   HBG712172
- OMA:   RIADEFP
- PhylomeDB:   Q9RAE7
- ProtClustDB:   CLSK881831
- BioCyc:   RLEG216596:PRL120416-MONOMER
- HAMAP:   MF_01201
- InterPro:   IPR000821
- InterPro:   IPR009006
- InterPro:   IPR011079
- InterPro:   IPR001608
- InterPro:   IPR020622
- Gene3D:   G3DSA:2.40.37.10
- PRINTS:   PR00992
- TIGRFAMs:   TIGR00492

Pfam domain/function: PF00842 Ala_racemase_C; PF01168 Ala_racemase_N; SSF50621 Racem_decarbox_C

EC number: =5.1.1.1

Molecular weight: Translated: 40549; Mature: 40549

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS00395 ALANINE_RACEMASE

Important sites: ACT_SITE 51-51 ACT_SITE 272-272

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDSDILVSRTRTATIAQGATGYLMIDLAALGRNYRKLVSMLAPVRAGAVVKADAYGLGAE
CCCCEEEECCCCEEEECCCCCEEEEEHHHHCCHHHHHHHHHHHHHCCCEEEECCCCCCHH
RVARTLYSEGCRHFFVAQFVEAVRLRPALAHDAQIFVLNGLQPGNEIACAEMGIVPVLNS
HHHHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCEEEEEECCCCCCCEEHHHHHHHHHHHH
LAQWRQWSAAARILKRCLPAVLQFDTGMSRLGFPREERRELAAALRDGSNVEILFIMSHL
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHCCCCEEEEEEEHHH
ASADDMGSEQNGEQFAEMSRIADEFPGFDISFANSGGVFLGEAYYGVLARPGIALYGGAP
CCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCEEEEHHHHHHHCCCCEEEECCCC
NAGEKNPMEPVVSLNVAVVQTRTVPAGAKVGYGGAHVTQRETRLATIAAGYADGLPRCLS
CCCCCCCCCHHHEEEEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHC
DRGAVYFKGVRLPIVGRVSMDSTTVDITALPEGALTFGSLVEVLGRHQTLEDIARDAGTI
CCCCEEEECEEEEEEEEEECCCCEEEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHCCCE
SYEILTGLGDRYDRQYR
EHHHHHHCCHHHCCCCC
>Mature Secondary Structure
MDSDILVSRTRTATIAQGATGYLMIDLAALGRNYRKLVSMLAPVRAGAVVKADAYGLGAE
CCCCEEEECCCCEEEECCCCCEEEEEHHHHCCHHHHHHHHHHHHHCCCEEEECCCCCCHH
RVARTLYSEGCRHFFVAQFVEAVRLRPALAHDAQIFVLNGLQPGNEIACAEMGIVPVLNS
HHHHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCEEEEEECCCCCCCEEHHHHHHHHHHHH
LAQWRQWSAAARILKRCLPAVLQFDTGMSRLGFPREERRELAAALRDGSNVEILFIMSHL
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHCCCCEEEEEEEHHH
ASADDMGSEQNGEQFAEMSRIADEFPGFDISFANSGGVFLGEAYYGVLARPGIALYGGAP
CCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCEEEEHHHHHHHCCCCEEEECCCC
NAGEKNPMEPVVSLNVAVVQTRTVPAGAKVGYGGAHVTQRETRLATIAAGYADGLPRCLS
CCCCCCCCCHHHEEEEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHC
DRGAVYFKGVRLPIVGRVSMDSTTVDITALPEGALTFGSLVEVLGRHQTLEDIARDAGTI
CCCCEEEECEEEEEEEEEECCCCEEEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHCCCE
SYEILTGLGDRYDRQYR
EHHHHHHCCHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10792736