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The map label for this gene is yhfT [H]

Identifier: 116249037

GI number: 116249037

Start: 396324

End: 397859

Strand: Direct

Name: yhfT [H]

Synonym: pRL120368

Alternate gene names: 116249037

Gene position: 396324-397859 (Clockwise)

Preceding gene: 116249036

Following gene: 116249038

Centisome position: 45.55

GC content: 61.52

Gene sequence:

>1536_bases
GTGTCCATGCCGATCCATGCGCAGCTTCTTCGACATGCAACGGAGACACCGGACAAGCCGGCGCTGGTGATCAACGGGCA
TTCGCTCAGCTATGGCGAGCTCTATGTGCGCGCCAAGGCAATCTATCGGTTTCTCCAAGATCTCCCTCGCTCGAACCGCC
GCACGCTCGATCTGCCCGGTGTGGAGACGCTTGCCGCTCTCAGCCTTAGCAACCATATCGGCTTTGCCGAGTATTTTGCG
GCGGCCACCGCTTTCCCGAATGCCTGCGCGGTCATCGACCCGATAATGCCGGCGGAAAGGATCGAACGCATCATCGAGCG
GCTGACGCCTGACGTTCTGATCGTCGACGACGATGCCGGGCCGAGCGCCGAAATTGCCCGTAGATTCGGCATTCCCGTCG
TCACCGCCGGAGCGGAGGCTTTCGACCTCGCTGCAGCGGGCGGTGAAGCAGAGCTGCCGGCCGATGCCGACAAGATTTTT
CTGATCGGCTTCACGTCAGGGACCACCGCCGAGCCGAAGGCCTATTATCGCTCGCGCGAGCAGTGGCGCTGGAGCCTCGA
TCGCGGACGCGTCGTTTTCGAGCTCGAAGACGCGCCGTCGACAATGTGCCCGGGAGCCCTCGCGCACGGCCTGGCGCTCT
ATGCATTGGTCGAAGCTCTTGATGCGGGGGCAACCTTCCATTCAGTCCGGAAATGGGATCCGGGCGTGGTCGCACGCACG
CTCTGCTCCTGGCAAGTCGAACGGCTGGTTGCCGTGCCCACCCATATAGCCGGAATTGCAAGGGCGTGGGCGGGCGAACC
CGCTTTGACCTTCTTGCGCGACGTCCTAACCGCCGGCGCAAAGCTTGACAGGAACGGGGTCGAATCCATGCGTCGCCTGT
TTCCGAAGGCCCGTATCCGGGAATATTACGGCGCATCCGAGATTGGTTTCATGACCGTCTCGACCCTTGCGGGCGGCGAG
ATCGATTTTCCGATCGACCGGGTCGGACAAGCCTATCCCGGTGTCGAAATCTCCATTCGCGATCCAGACGAGAATAATGT
CGGGACCGATGTGCCAGGCACCATTTTCGTCAGGAGCGACCTGATCGCGGATGGATATCTCTGGGGCGACGACGGTCAGG
CCTTCCGGGTCACAGAAGCCGGAGCGACGGTCGGAGATCTCGGCGAACTCGATGCCAATGGCATGCTGCGGGTGATCGGC
AGGGCGGGCGGCATGATGATATCAGGCGGCAACAACGTTTATCCCGCCGAAGTCGAGAGTGCGCTCAAGGCCTGCCCGGG
GGTAGAGGATGCTGTTGTCTTCGCGCTGCCGGACGCCTATTTCGGCCAACGGATCGTCGCTGTCGTCTCGGGAGAGACGG
TGGGCCCGAAAATCCTGGCTGATCATTGCGCGCAGATGCTCGGGAAGTACAAGATCCCCAAGCAATTCTACCATATAAGG
TCCTGGCCGATGACGAGCAGCGGCAAGATTTCGAGGGGGCAAGTGGAGCGGGCCGTGGTTTCTGGAGACGATCTTGTCCT
ACGCCTTTCAGCCTGA

Upstream 100 bases:

>100_bases
GGGTCTGCGTCGGCCTCTCCGACAGCCGCCCGACGCCGATCCCCGAAGCGACCAGAGCCGCTCTGTCTCCAGGCTGACGG
CCGATCATTCAAACCAGCGG

Downstream 100 bases:

>100_bases
CGAGGATTGGCAGCCAGTCGTGGTTGCCGCCTACCGGTCGCCGATCGGCCGGGCTTTCGGATCGCTTGCGACTGTCGCGG
CGGAAGACCTGCTTGCGCCG

Product: hypothetical protein

Products: AMP; diphosphate +an acyl-CoA [C]

Alternate protein names: NA

Number of amino acids: Translated: 511; Mature: 510

Protein sequence:

>511_residues
MSMPIHAQLLRHATETPDKPALVINGHSLSYGELYVRAKAIYRFLQDLPRSNRRTLDLPGVETLAALSLSNHIGFAEYFA
AATAFPNACAVIDPIMPAERIERIIERLTPDVLIVDDDAGPSAEIARRFGIPVVTAGAEAFDLAAAGGEAELPADADKIF
LIGFTSGTTAEPKAYYRSREQWRWSLDRGRVVFELEDAPSTMCPGALAHGLALYALVEALDAGATFHSVRKWDPGVVART
LCSWQVERLVAVPTHIAGIARAWAGEPALTFLRDVLTAGAKLDRNGVESMRRLFPKARIREYYGASEIGFMTVSTLAGGE
IDFPIDRVGQAYPGVEISIRDPDENNVGTDVPGTIFVRSDLIADGYLWGDDGQAFRVTEAGATVGDLGELDANGMLRVIG
RAGGMMISGGNNVYPAEVESALKACPGVEDAVVFALPDAYFGQRIVAVVSGETVGPKILADHCAQMLGKYKIPKQFYHIR
SWPMTSSGKISRGQVERAVVSGDDLVLRLSA

Sequences:

>Translated_511_residues
MSMPIHAQLLRHATETPDKPALVINGHSLSYGELYVRAKAIYRFLQDLPRSNRRTLDLPGVETLAALSLSNHIGFAEYFA
AATAFPNACAVIDPIMPAERIERIIERLTPDVLIVDDDAGPSAEIARRFGIPVVTAGAEAFDLAAAGGEAELPADADKIF
LIGFTSGTTAEPKAYYRSREQWRWSLDRGRVVFELEDAPSTMCPGALAHGLALYALVEALDAGATFHSVRKWDPGVVART
LCSWQVERLVAVPTHIAGIARAWAGEPALTFLRDVLTAGAKLDRNGVESMRRLFPKARIREYYGASEIGFMTVSTLAGGE
IDFPIDRVGQAYPGVEISIRDPDENNVGTDVPGTIFVRSDLIADGYLWGDDGQAFRVTEAGATVGDLGELDANGMLRVIG
RAGGMMISGGNNVYPAEVESALKACPGVEDAVVFALPDAYFGQRIVAVVSGETVGPKILADHCAQMLGKYKIPKQFYHIR
SWPMTSSGKISRGQVERAVVSGDDLVLRLSA
>Mature_510_residues
SMPIHAQLLRHATETPDKPALVINGHSLSYGELYVRAKAIYRFLQDLPRSNRRTLDLPGVETLAALSLSNHIGFAEYFAA
ATAFPNACAVIDPIMPAERIERIIERLTPDVLIVDDDAGPSAEIARRFGIPVVTAGAEAFDLAAAGGEAELPADADKIFL
IGFTSGTTAEPKAYYRSREQWRWSLDRGRVVFELEDAPSTMCPGALAHGLALYALVEALDAGATFHSVRKWDPGVVARTL
CSWQVERLVAVPTHIAGIARAWAGEPALTFLRDVLTAGAKLDRNGVESMRRLFPKARIREYYGASEIGFMTVSTLAGGEI
DFPIDRVGQAYPGVEISIRDPDENNVGTDVPGTIFVRSDLIADGYLWGDDGQAFRVTEAGATVGDLGELDANGMLRVIGR
AGGMMISGGNNVYPAEVESALKACPGVEDAVVFALPDAYFGQRIVAVVSGETVGPKILADHCAQMLGKYKIPKQFYHIRS
WPMTSSGKISRGQVERAVVSGDDLVLRLSA

Specific function: May be involved in fatty acid metabolism [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=223, Percent_Identity=26.0089686098655, Blast_Score=90, Evalue=5e-18,
Organism=Homo sapiens, GI157311624, Length=351, Percent_Identity=25.3561253561254, Blast_Score=86, Evalue=7e-17,
Organism=Homo sapiens, GI157311622, Length=351, Percent_Identity=25.3561253561254, Blast_Score=86, Evalue=7e-17,
Organism=Homo sapiens, GI58082049, Length=351, Percent_Identity=25.6410256410256, Blast_Score=86, Evalue=1e-16,
Organism=Homo sapiens, GI115511026, Length=360, Percent_Identity=25.5555555555556, Blast_Score=77, Evalue=4e-14,
Organism=Homo sapiens, GI38505220, Length=362, Percent_Identity=25.1381215469613, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI187761345, Length=538, Percent_Identity=24.1635687732342, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI187761343, Length=538, Percent_Identity=24.1635687732342, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI122937307, Length=237, Percent_Identity=28.6919831223629, Blast_Score=71, Evalue=3e-12,
Organism=Homo sapiens, GI42544132, Length=375, Percent_Identity=22.6666666666667, Blast_Score=70, Evalue=4e-12,
Organism=Escherichia coli, GI1788107, Length=197, Percent_Identity=26.9035532994924, Blast_Score=82, Evalue=9e-17,
Organism=Escherichia coli, GI145693145, Length=357, Percent_Identity=24.0896358543417, Blast_Score=72, Evalue=9e-14,
Organism=Escherichia coli, GI221142682, Length=366, Percent_Identity=25.9562841530055, Blast_Score=69, Evalue=8e-13,
Organism=Escherichia coli, GI1790505, Length=382, Percent_Identity=23.8219895287958, Blast_Score=63, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17557194, Length=369, Percent_Identity=26.2872628726287, Blast_Score=108, Evalue=9e-24,
Organism=Caenorhabditis elegans, GI17538037, Length=374, Percent_Identity=25.4010695187166, Blast_Score=102, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17558820, Length=354, Percent_Identity=27.4011299435028, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17531443, Length=365, Percent_Identity=23.013698630137, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI32563687, Length=344, Percent_Identity=27.6162790697674, Blast_Score=85, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI17560308, Length=402, Percent_Identity=26.3681592039801, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI71994690, Length=203, Percent_Identity=27.0935960591133, Blast_Score=77, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI71994694, Length=203, Percent_Identity=27.0935960591133, Blast_Score=76, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI71994703, Length=203, Percent_Identity=27.0935960591133, Blast_Score=76, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI71985884, Length=344, Percent_Identity=24.7093023255814, Blast_Score=75, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17559526, Length=364, Percent_Identity=23.0769230769231, Blast_Score=75, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6319699, Length=362, Percent_Identity=26.2430939226519, Blast_Score=86, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24581924, Length=409, Percent_Identity=25.4278728606357, Blast_Score=99, Evalue=5e-21,
Organism=Drosophila melanogaster, GI18859661, Length=358, Percent_Identity=23.463687150838, Blast_Score=86, Evalue=5e-17,
Organism=Drosophila melanogaster, GI20130357, Length=264, Percent_Identity=23.4848484848485, Blast_Score=77, Evalue=4e-14,
Organism=Drosophila melanogaster, GI19922652, Length=243, Percent_Identity=27.5720164609054, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI21356947, Length=220, Percent_Identity=25.4545454545455, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24653035, Length=262, Percent_Identity=23.2824427480916, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3 [C]

Molecular weight: Translated: 55073; Mature: 54942

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMPIHAQLLRHATETPDKPALVINGHSLSYGELYVRAKAIYRFLQDLPRSNRRTLDLPG
CCCCHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEECCC
VETLAALSLSNHIGFAEYFAAATAFPNACAVIDPIMPAERIERIIERLTPDVLIVDDDAG
HHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHCCCCCHHHHHHHHHHCCCCEEEEECCCC
PSAEIARRFGIPVVTAGAEAFDLAAAGGEAELPADADKIFLIGFTSGTTAEPKAYYRSRE
CHHHHHHHCCCCEEECCCCHHHHEECCCCCCCCCCCCEEEEEEECCCCCCCCHHHHHCHH
QWRWSLDRGRVVFELEDAPSTMCPGALAHGLALYALVEALDAGATFHSVRKWDPGVVART
HHEEECCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCHHHHHH
LCSWQVERLVAVPTHIAGIARAWAGEPALTFLRDVLTAGAKLDRNGVESMRRLFPKARIR
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
EYYGASEIGFMTVSTLAGGEIDFPIDRVGQAYPGVEISIRDPDENNVGTDVPGTIFVRSD
HHCCCCCCCEEEEEECCCCCCCCCHHHHCCCCCCCEEEEECCCCCCCCCCCCCEEEEEEC
LIADGYLWGDDGQAFRVTEAGATVGDLGELDANGMLRVIGRAGGMMISGGNNVYPAEVES
EEECCEEECCCCCEEEEEECCCCCCCCCCCCCCCCEEEEECCCCEEEECCCCCCHHHHHH
ALKACPGVEDAVVFALPDAYFGQRIVAVVSGETVGPKILADHCAQMLGKYKIPKQFYHIR
HHHHCCCCCCEEEEEECCHHHCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCCHHHHHHH
SWPMTSSGKISRGQVERAVVSGDDLVLRLSA
CCCCCCCCCCCCCHHHHHEECCCCEEEEECC
>Mature Secondary Structure 
SMPIHAQLLRHATETPDKPALVINGHSLSYGELYVRAKAIYRFLQDLPRSNRRTLDLPG
CCCHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEECCC
VETLAALSLSNHIGFAEYFAAATAFPNACAVIDPIMPAERIERIIERLTPDVLIVDDDAG
HHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHCCCCCHHHHHHHHHHCCCCEEEEECCCC
PSAEIARRFGIPVVTAGAEAFDLAAAGGEAELPADADKIFLIGFTSGTTAEPKAYYRSRE
CHHHHHHHCCCCEEECCCCHHHHEECCCCCCCCCCCCEEEEEEECCCCCCCCHHHHHCHH
QWRWSLDRGRVVFELEDAPSTMCPGALAHGLALYALVEALDAGATFHSVRKWDPGVVART
HHEEECCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCHHHHHH
LCSWQVERLVAVPTHIAGIARAWAGEPALTFLRDVLTAGAKLDRNGVESMRRLFPKARIR
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
EYYGASEIGFMTVSTLAGGEIDFPIDRVGQAYPGVEISIRDPDENNVGTDVPGTIFVRSD
HHCCCCCCCEEEEEECCCCCCCCCHHHHCCCCCCCEEEEECCCCCCCCCCCCCEEEEEEC
LIADGYLWGDDGQAFRVTEAGATVGDLGELDANGMLRVIGRAGGMMISGGNNVYPAEVES
EEECCEEECCCCCEEEEEECCCCCCCCCCCCCCCCEEEEECCCCEEEECCCCCCHHHHHH
ALKACPGVEDAVVFALPDAYFGQRIVAVVSGETVGPKILADHCAQMLGKYKIPKQFYHIR
HHHHCCCCCCEEEEEECCHHHCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCCHHHHHHH
SWPMTSSGKISRGQVERAVVSGDDLVLRLSA
CCCCCCCCCCCCCHHHHHEECCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0024 {myristate}} 0.0021 {myristate}} 0.0012 {myristate}} 0.0002 {myristate}} 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0112 {hexanoate}} 0.0083 {decanoate}} 0.0063 {decanoate}} 0.0043 {dec

Substrates: ATP; a long-chain carboxylic acid; CoA [C]

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate +an acyl-CoA [C]

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]