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The map label for this gene is vraB [H]

Identifier: 116249038

GI number: 116249038

Start: 397837

End: 399042

Strand: Direct

Name: vraB [H]

Synonym: pRL120369

Alternate gene names: 116249038

Gene position: 397837-399042 (Clockwise)

Preceding gene: 116249037

Following gene: 116249039

Centisome position: 45.73

GC content: 64.84

Gene sequence:

>1206_bases
TTGTCCTACGCCTTTCAGCCTGACGAGGATTGGCAGCCAGTCGTGGTTGCCGCCTACCGGTCGCCGATCGGCCGGGCTTT
CGGATCGCTTGCGACTGTCGCGGCGGAAGACCTGCTTGCGCCGATCATTCATCGGATCCTTTCCGAAACGGGAATTGCGC
CCGAAAGCATCGACGACGTTCTGGTGGGCAACGCCGCAGGCGGCGGCGGAAACATCGCGCGGCTGGCAGCGCTGACCGCA
GGTCTGCCCGTGGCGGTTCCCGGTGTTTCGATCGATCGGCAATGCGGCTCGGGCCTGGAAGCGATCATCATGGCAGCCCG
GCAGATCCAGGCGAAAGCCGGTTCGTGCTTTCTGGCCGGTGGCGTGGAGAGCGTCAGCACCGCGCCATGGCGCGTCGAAA
GACCAAAGGCGAATGGGATGCCGCCGCGATTTTACGGGCGCGCCCGGTTTTCGCCTGACACGATCGGCGACCCGGAAATG
GGGATTGCCGCCGAAAACGTCGCCCGTCAGTTCGGCATATCGCGGCAGAGACAGGATGAATTCGCCCTGCGCAGCCACCG
GCTCGCAGTAGCGGCGGCCGAGGCAGGCATCTTTCGCCCCGAGATCGTCGACATTTCCACCGGCCACGGTCTGATCGAAC
GGGACGAGTGCCCGCGCCCGTCCACATCTCTCGAGGCGCTCGCAAACCTCCGGCCGGTCTTCCTGGCCGATGGTTCGGTG
ACAGCCGGCAATGCCTGCCCGCTGAACGACGGCGCCTGTCTGGTGCTGGTCATGAGCCGCGCAATGGCGAGAAGCATGGG
CATCGAAAAGGGCCTTGCCTTCATCGACAGTGCCGCTGCCGGTGTGGATCCCAATCTGCTCGGCATCGGCCCGGTCGCCT
CGACCAGAAAGCTATTGCAGCGTCAGCCCGGGCTCTCACTTTCCGACGTCAATGCCATCGAATTCAACGAGGCCTTCGTG
GCCCAGGTTCTGGCATCGCTGGACCAGCTCGCTATCCCTGCCGACGCGATCAACAAGGACGGCGGAGCCATTGCGCTCGG
CCATCCGTTCGGGGCGTCCGGCGCAATCCTCGTTACCAGGCTATACAGCCGGCTGATCCGCAACACCGGGGAAGGCTGGG
CGCCCGGCGCGACTGCCTTGGCCATGATCGGCATCGGCGGAGGCATCGGACTGACCGCCATGTTCGAGGCTACCCAGCTT
TCTTGA

Upstream 100 bases:

>100_bases
ATCCCCAAGCAATTCTACCATATAAGGTCCTGGCCGATGACGAGCAGCGGCAAGATTTCGAGGGGGCAAGTGGAGCGGGC
CGTGGTTTCTGGAGACGATC

Downstream 100 bases:

>100_bases
CCTGCCGAAGGCGCAGAACCGTCAGGCGGCGGCGCGTTTGTGATCCGCCGGATGTTGAAGCCGGAAGCCGATCGAGAGCG
ACTCCCGCCATGCGTTTGGA

Product: putative thiolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 401; Mature: 400

Protein sequence:

>401_residues
MSYAFQPDEDWQPVVVAAYRSPIGRAFGSLATVAAEDLLAPIIHRILSETGIAPESIDDVLVGNAAGGGGNIARLAALTA
GLPVAVPGVSIDRQCGSGLEAIIMAARQIQAKAGSCFLAGGVESVSTAPWRVERPKANGMPPRFYGRARFSPDTIGDPEM
GIAAENVARQFGISRQRQDEFALRSHRLAVAAAEAGIFRPEIVDISTGHGLIERDECPRPSTSLEALANLRPVFLADGSV
TAGNACPLNDGACLVLVMSRAMARSMGIEKGLAFIDSAAAGVDPNLLGIGPVASTRKLLQRQPGLSLSDVNAIEFNEAFV
AQVLASLDQLAIPADAINKDGGAIALGHPFGASGAILVTRLYSRLIRNTGEGWAPGATALAMIGIGGGIGLTAMFEATQL
S

Sequences:

>Translated_401_residues
MSYAFQPDEDWQPVVVAAYRSPIGRAFGSLATVAAEDLLAPIIHRILSETGIAPESIDDVLVGNAAGGGGNIARLAALTA
GLPVAVPGVSIDRQCGSGLEAIIMAARQIQAKAGSCFLAGGVESVSTAPWRVERPKANGMPPRFYGRARFSPDTIGDPEM
GIAAENVARQFGISRQRQDEFALRSHRLAVAAAEAGIFRPEIVDISTGHGLIERDECPRPSTSLEALANLRPVFLADGSV
TAGNACPLNDGACLVLVMSRAMARSMGIEKGLAFIDSAAAGVDPNLLGIGPVASTRKLLQRQPGLSLSDVNAIEFNEAFV
AQVLASLDQLAIPADAINKDGGAIALGHPFGASGAILVTRLYSRLIRNTGEGWAPGATALAMIGIGGGIGLTAMFEATQL
S
>Mature_400_residues
SYAFQPDEDWQPVVVAAYRSPIGRAFGSLATVAAEDLLAPIIHRILSETGIAPESIDDVLVGNAAGGGGNIARLAALTAG
LPVAVPGVSIDRQCGSGLEAIIMAARQIQAKAGSCFLAGGVESVSTAPWRVERPKANGMPPRFYGRARFSPDTIGDPEMG
IAAENVARQFGISRQRQDEFALRSHRLAVAAAEAGIFRPEIVDISTGHGLIERDECPRPSTSLEALANLRPVFLADGSVT
AGNACPLNDGACLVLVMSRAMARSMGIEKGLAFIDSAAAGVDPNLLGIGPVASTRKLLQRQPGLSLSDVNAIEFNEAFVA
QVLASLDQLAIPADAINKDGGAIALGHPFGASGAILVTRLYSRLIRNTGEGWAPGATALAMIGIGGGIGLTAMFEATQLS

Specific function: Thiolytic Cleavage Of Beta-Ketoadipyl-CoA To Succinate And Acetyl-CoA (By Similarity). [C]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI148539872, Length=392, Percent_Identity=38.5204081632653, Blast_Score=225, Evalue=6e-59,
Organism=Homo sapiens, GI4501853, Length=395, Percent_Identity=36.2025316455696, Blast_Score=217, Evalue=2e-56,
Organism=Homo sapiens, GI167614485, Length=395, Percent_Identity=35.4430379746835, Blast_Score=205, Evalue=7e-53,
Organism=Homo sapiens, GI4557237, Length=409, Percent_Identity=33.2518337408313, Blast_Score=182, Evalue=4e-46,
Organism=Homo sapiens, GI4504327, Length=400, Percent_Identity=31.25, Blast_Score=159, Evalue=4e-39,
Organism=Homo sapiens, GI194353979, Length=386, Percent_Identity=28.4974093264249, Blast_Score=126, Evalue=5e-29,
Organism=Escherichia coli, GI1787663, Length=401, Percent_Identity=41.645885286783, Blast_Score=242, Evalue=4e-65,
Organism=Escherichia coli, GI87082165, Length=399, Percent_Identity=40.8521303258145, Blast_Score=239, Evalue=2e-64,
Organism=Escherichia coli, GI1788554, Length=397, Percent_Identity=42.0654911838791, Blast_Score=234, Evalue=6e-63,
Organism=Escherichia coli, GI48994986, Length=401, Percent_Identity=38.9027431421446, Blast_Score=212, Evalue=4e-56,
Organism=Escherichia coli, GI1788683, Length=402, Percent_Identity=30.3482587064677, Blast_Score=142, Evalue=5e-35,
Organism=Caenorhabditis elegans, GI133906874, Length=393, Percent_Identity=35.3689567430025, Blast_Score=217, Evalue=1e-56,
Organism=Caenorhabditis elegans, GI25147385, Length=368, Percent_Identity=34.7826086956522, Blast_Score=183, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI17535921, Length=395, Percent_Identity=32.6582278481013, Blast_Score=172, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI17551802, Length=431, Percent_Identity=32.0185614849188, Blast_Score=155, Evalue=3e-38,
Organism=Caenorhabditis elegans, GI17535917, Length=363, Percent_Identity=28.3746556473829, Blast_Score=106, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6322031, Length=394, Percent_Identity=36.0406091370558, Blast_Score=196, Evalue=6e-51,
Organism=Saccharomyces cerevisiae, GI6325229, Length=373, Percent_Identity=31.3672922252011, Blast_Score=146, Evalue=6e-36,
Organism=Drosophila melanogaster, GI24655093, Length=392, Percent_Identity=36.4795918367347, Blast_Score=221, Evalue=6e-58,
Organism=Drosophila melanogaster, GI17648125, Length=395, Percent_Identity=34.1772151898734, Blast_Score=188, Evalue=5e-48,
Organism=Drosophila melanogaster, GI24640423, Length=373, Percent_Identity=35.1206434316354, Blast_Score=179, Evalue=2e-45,
Organism=Drosophila melanogaster, GI17137578, Length=401, Percent_Identity=30.9226932668329, Blast_Score=152, Evalue=3e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: 2.3.1.- [C]

Molecular weight: Translated: 41471; Mature: 41340

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: PS00737 THIOLASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYAFQPDEDWQPVVVAAYRSPIGRAFGSLATVAAEDLLAPIIHRILSETGIAPESIDDV
CCCCCCCCCCCCCEEEEEHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHH
LVGNAAGGGGNIARLAALTAGLPVAVPGVSIDRQCGSGLEAIIMAARQIQAKAGSCFLAG
EECCCCCCCCCHHHHHHHHCCCCEEECCCCCCHHHCCCHHHHHHHHHHHHHCCCCEEEEC
GVESVSTAPWRVERPKANGMPPRFYGRARFSPDTIGDPEMGIAAENVARQFGISRQRQDE
CCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHH
FALRSHRLAVAAAEAGIFRPEIVDISTGHGLIERDECPRPSTSLEALANLRPVFLADGSV
HHHHHHHHEEEEHHCCCCCCEEEEECCCCCCEECCCCCCCCHHHHHHHCCCEEEEECCCE
TAGNACPLNDGACLVLVMSRAMARSMGIEKGLAFIDSAAAGVDPNLLGIGPVASTRKLLQ
ECCCCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCEECCCCHHHHHHHH
RQPGLSLSDVNAIEFNEAFVAQVLASLDQLAIPADAINKDGGAIALGHPFGASGAILVTR
HCCCCCCCCCCEEEHHHHHHHHHHHHHHHHCCCHHHCCCCCCEEEECCCCCCCHHHHHHH
LYSRLIRNTGEGWAPGATALAMIGIGGGIGLTAMFEATQLS
HHHHHHHCCCCCCCCCHHHEEEEECCCCCCHHHEEHHHCCC
>Mature Secondary Structure 
SYAFQPDEDWQPVVVAAYRSPIGRAFGSLATVAAEDLLAPIIHRILSETGIAPESIDDV
CCCCCCCCCCCCEEEEEHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHH
LVGNAAGGGGNIARLAALTAGLPVAVPGVSIDRQCGSGLEAIIMAARQIQAKAGSCFLAG
EECCCCCCCCCHHHHHHHHCCCCEEECCCCCCHHHCCCHHHHHHHHHHHHHCCCCEEEEC
GVESVSTAPWRVERPKANGMPPRFYGRARFSPDTIGDPEMGIAAENVARQFGISRQRQDE
CCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHH
FALRSHRLAVAAAEAGIFRPEIVDISTGHGLIERDECPRPSTSLEALANLRPVFLADGSV
HHHHHHHHEEEEHHCCCCCCEEEEECCCCCCEECCCCCCCCHHHHHHHCCCEEEEECCCE
TAGNACPLNDGACLVLVMSRAMARSMGIEKGLAFIDSAAAGVDPNLLGIGPVASTRKLLQ
ECCCCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCEECCCCHHHHHHHH
RQPGLSLSDVNAIEFNEAFVAQVLASLDQLAIPADAINKDGGAIALGHPFGASGAILVTR
HCCCCCCCCCCEEEHHHHHHHHHHHHHHHHCCCHHHCCCCCCEEEECCCCCCCHHHHHHH
LYSRLIRNTGEGWAPGATALAMIGIGGGIGLTAMFEATQLS
HHHHHHHCCCCCCCCCHHHEEEEECCCCCCHHHEEHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA