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The map label for this gene is chqB [H]

Identifier: 116248884

GI number: 116248884

Start: 222089

End: 222937

Strand: Reverse

Name: chqB [H]

Synonym: pRL120214

Alternate gene names: 116248884

Gene position: 222937-222089 (Counterclockwise)

Preceding gene: 116248885

Following gene: 116248883

Centisome position: 25.62

GC content: 58.3

Gene sequence:

>849_bases
GTGCCTGAATATTTCAGCGAAGAGAGATCCGTGGAAGCCGTCAACTCGCGGATGGGGCAGGACATTAATCCGCGCCTGGC
CGAAGTCATGGCGTCGCTTGTCAGGCACCTGCATGCCTTTGCCAAGGATGTCAGCCTGACCCAGGAGGAATGGGAGCTGG
CCATAGGCTTCCTGACCCGCAGCGGCCATCTCTGTCATGATGAGCGGCAGGAGTTCATCCTGCTCAGCGATACGCTGGGT
TTGTCGATGCTGGTGGATGCGATCAACAACAGGCGCCCGCCAGGCGCCACGGAAAATACGGTGCTTGGACCGTTCCATGT
CGAAGGGGCGCCCGTACGGCAGATGGGTGAAAACATCTCGCTCGACGGCAAGGGCGAGAGCTGTCTCTTCATCGGCCGGG
TGCTGGATCTTGACGGCAATCCGATCGAGGGAGCTCGGATCGATATATGGTCGGATAATTCCGATGGCTTCTACGACGTC
CAGCAGCCGGATATCCAGCCGAAGTGGAACAACCGGGGCATTTTCGTCACCGGCGCAGATGGCGCCTATAGCTTCGTCGG
CATAAAGCCGGTTTCCTATCCGATCCCGGATGACGGCCCGGTCGGCCAGATGTTGACGTCCCTCGGCCGCCATCCCTACC
GTCCGGCCCATACCCATTACCTGATCACGGCTTCAGGTTATCAGAAGCTCGTAACCCACACGTTTGTCGGCGACGATCCC
TATCTTGAATCCGATACGGTGTTTGGTGTCAAAAACAGCCTGGTCGCGCCTTTCGAGCGTGTCGATCATCCCACGGTCTG
GCGCTCCGATTTCGATTTCGTGCTGACGCCGGTTGAGAATAGACAATGA

Upstream 100 bases:

>100_bases
ACTGGAACCCGCGCCCGATCGAACGGGAAGCGATCCGGGCTTTGTTGCAGAGCGCCTGGGAGGGCGCGCGGCCGCGTTAA
TACCTTAGGAGGATGGCCAC

Downstream 100 bases:

>100_bases
TCGTCGCCCGCTACGCGGTATCCGACCCCGGCAAGGCTGCCGAACGCTCCCGATTGATCGAGGAGCACAAGGCCTATCTG
CATGGCGCGGCGATCCGCAT

Product: putative dioxygenase

Products: NA

Alternate protein names: 1,2-HQD [H]

Number of amino acids: Translated: 282; Mature: 281

Protein sequence:

>282_residues
MPEYFSEERSVEAVNSRMGQDINPRLAEVMASLVRHLHAFAKDVSLTQEEWELAIGFLTRSGHLCHDERQEFILLSDTLG
LSMLVDAINNRRPPGATENTVLGPFHVEGAPVRQMGENISLDGKGESCLFIGRVLDLDGNPIEGARIDIWSDNSDGFYDV
QQPDIQPKWNNRGIFVTGADGAYSFVGIKPVSYPIPDDGPVGQMLTSLGRHPYRPAHTHYLITASGYQKLVTHTFVGDDP
YLESDTVFGVKNSLVAPFERVDHPTVWRSDFDFVLTPVENRQ

Sequences:

>Translated_282_residues
MPEYFSEERSVEAVNSRMGQDINPRLAEVMASLVRHLHAFAKDVSLTQEEWELAIGFLTRSGHLCHDERQEFILLSDTLG
LSMLVDAINNRRPPGATENTVLGPFHVEGAPVRQMGENISLDGKGESCLFIGRVLDLDGNPIEGARIDIWSDNSDGFYDV
QQPDIQPKWNNRGIFVTGADGAYSFVGIKPVSYPIPDDGPVGQMLTSLGRHPYRPAHTHYLITASGYQKLVTHTFVGDDP
YLESDTVFGVKNSLVAPFERVDHPTVWRSDFDFVLTPVENRQ
>Mature_281_residues
PEYFSEERSVEAVNSRMGQDINPRLAEVMASLVRHLHAFAKDVSLTQEEWELAIGFLTRSGHLCHDERQEFILLSDTLGL
SMLVDAINNRRPPGATENTVLGPFHVEGAPVRQMGENISLDGKGESCLFIGRVLDLDGNPIEGARIDIWSDNSDGFYDVQ
QPDIQPKWNNRGIFVTGADGAYSFVGIKPVSYPIPDDGPVGQMLTSLGRHPYRPAHTHYLITASGYQKLVTHTFVGDDPY
LESDTVFGVKNSLVAPFERVDHPTVWRSDFDFVLTPVENRQ

Specific function: Catalyzes the ortho-cleavage of the aromatic ring of hydroxyquinol [H]

COG id: COG3485

COG function: function code Q; Protocatechuate 3,4-dioxygenase beta subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the intradiol ring-cleavage dioxygenase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007535
- InterPro:   IPR000627
- InterPro:   IPR015889 [H]

Pfam domain/function: PF00775 Dioxygenase_C; PF04444 Dioxygenase_N [H]

EC number: =1.13.11.37 [H]

Molecular weight: Translated: 31455; Mature: 31323

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: PS00083 INTRADIOL_DIOXYGENAS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEYFSEERSVEAVNSRMGQDINPRLAEVMASLVRHLHAFAKDVSLTQEEWELAIGFLTR
CCCHHCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHEEEEEEEC
SGHLCHDERQEFILLSDTLGLSMLVDAINNRRPPGATENTVLGPFHVEGAPVRQMGENIS
CCCCCCCCCCCEEEEECCCCHHHHHHHHHCCCCCCCCCCCEECEEECCCCCHHHCCCCCE
LDGKGESCLFIGRVLDLDGNPIEGARIDIWSDNSDGFYDVQQPDIQPKWNNRGIFVTGAD
ECCCCCCEEEEEEEEECCCCCCCCCEEEEEECCCCCEEECCCCCCCCCCCCCEEEEEECC
GAYSFVGIKPVSYPIPDDGPVGQMLTSLGRHPYRPAHTHYLITASGYQKLVTHTFVGDDP
CCEEEEEECCCCCCCCCCCCHHHHHHHHCCCCCCCCCCEEEEEECCHHHHHHHHCCCCCC
YLESDTVFGVKNSLVAPFERVDHPTVWRSDFDFVLTPVENRQ
CCCCCEEECCCCCCCCHHHHCCCCEEECCCCCEEEECCCCCC
>Mature Secondary Structure 
PEYFSEERSVEAVNSRMGQDINPRLAEVMASLVRHLHAFAKDVSLTQEEWELAIGFLTR
CCHHCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHEEEEEEEC
SGHLCHDERQEFILLSDTLGLSMLVDAINNRRPPGATENTVLGPFHVEGAPVRQMGENIS
CCCCCCCCCCCEEEEECCCCHHHHHHHHHCCCCCCCCCCCEECEEECCCCCHHHCCCCCE
LDGKGESCLFIGRVLDLDGNPIEGARIDIWSDNSDGFYDVQQPDIQPKWNNRGIFVTGAD
ECCCCCCEEEEEEEEECCCCCCCCCEEEEEECCCCCEEECCCCCCCCCCCCCEEEEEECC
GAYSFVGIKPVSYPIPDDGPVGQMLTSLGRHPYRPAHTHYLITASGYQKLVTHTFVGDDP
CCEEEEEECCCCCCCCCCCCHHHHHHHHCCCCCCCCCCEEEEEECCHHHHHHHHCCCCCC
YLESDTVFGVKNSLVAPFERVDHPTVWRSDFDFVLTPVENRQ
CCCCCEEECCCCCCCCHHHHCCCCEEECCCCCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9141483 [H]