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The map label for this gene is rbtR [H]

Identifier: 116248860

GI number: 116248860

Start: 195660

End: 196718

Strand: Reverse

Name: rbtR [H]

Synonym: pRL120189

Alternate gene names: 116248860

Gene position: 196718-195660 (Counterclockwise)

Preceding gene: 116248867

Following gene: 116248858

Centisome position: 22.61

GC content: 62.13

Gene sequence:

>1059_bases
ATGACCGACATCGGCAAGAAGAAGGCGACGATCTACGATCTCTCGATGCTATCGGGTGCGTCGCCTTCCACGGTGAGCGC
CGTGCTGAACGGAAGCTGGCGGAAGCGGCGTATCAAGGAGGCGACGGCGCAGCTCATTCAGAAGCTGGCAAACGAGCACG
GCTATACGGCAAACCTGCAGGCCCGTGGCCTGCGTTCGTCCCGCTCCGGTCTCGTCGGCCTTCTGCTGCCGGTGCACGAC
AACCGCTATTTCTCCTCGATGGCCCAGTCTTTCGAGGCGCAGGTCAGAAGCCGCGGCCAGTGCCCCCTCGTTGTCAGCGC
CTGTCGCGATCCGGAGGAGGAGCGCAAGGTCGTCGCGACGCTGATCTCCTATTCGATCGACGAGCTTTTCATCGCCGGCG
CAACCGATCCGGATGGCGTGCACAAGGTCTGCGAAAAAGCAGGGCTGAAGCACGTCAACATCGATCTGCCCGGCACGCTG
GCGCCATCGATCATCAGCGACAATCATGAAGGTGCGCGCATGCTGACACAGGCGATCATCGAGCGCGCGCAGGAGGATGG
ACCGCTTTCACCTGACGATCTGTACCTGTTCGGCGGCCGCAACGACCATGCCAGCCATGAGCGAATTCGCGGCTTCCGCG
ACGTAAAGCGCGCATTACTGGGCGCGGATCCCGACGTCTGCATCCAGCCGACCGGCTATTCACCCGCCATGACGGCGCGT
GCCTTCGAAGCCTTCTACGAAGGCCACGGAAAGCTGCCGCGCGCATTCTTCGTCAATTCCTCGATCAATCTCGAAGGGCT
GATGCGCTTCCTCGCCCTGCATCCGCACGAGACCTTCGCCGATATCATCGTCGGTTGCTACGACTACGATCCCTTCGGCT
CTTTCCTGCCTTTCCCCGTCTTCATGATCCGGCAGGACAGCGAAACGATGATCACCAAGGGGTTTGCGATCCTCGATGCG
GACCGCCGTCCGCCAATCACGCATCTGGTGCGGCCGACACTCGTGCCACCCCGCACGGCACTGACCGGCCCGCTCGACGC
GCTGAAGGATATCGAGTAG

Upstream 100 bases:

>100_bases
TCTTTTTTGCACAATCGATGGTGCAATGACTATCGATCAAGGTAGGGCTTTCGTCAAGAGGGGTTTGTTTCTAAGGGAAG
CGACAAGGGAAAGGCGAGAT

Downstream 100 bases:

>100_bases
CGCGCGCCGCCTCGTTGGAAACGCTGGGCTGACGGAGAACAGACCCTTTGATGCCCGATCGCGTTGGCATCAACAATTGC
CTTATCGGATCTAGCTTTCT

Product: putative transcriptional regulator

Products: NA

Alternate protein names: Rbt operon repressor [H]

Number of amino acids: Translated: 352; Mature: 351

Protein sequence:

>352_residues
MTDIGKKKATIYDLSMLSGASPSTVSAVLNGSWRKRRIKEATAQLIQKLANEHGYTANLQARGLRSSRSGLVGLLLPVHD
NRYFSSMAQSFEAQVRSRGQCPLVVSACRDPEEERKVVATLISYSIDELFIAGATDPDGVHKVCEKAGLKHVNIDLPGTL
APSIISDNHEGARMLTQAIIERAQEDGPLSPDDLYLFGGRNDHASHERIRGFRDVKRALLGADPDVCIQPTGYSPAMTAR
AFEAFYEGHGKLPRAFFVNSSINLEGLMRFLALHPHETFADIIVGCYDYDPFGSFLPFPVFMIRQDSETMITKGFAILDA
DRRPPITHLVRPTLVPPRTALTGPLDALKDIE

Sequences:

>Translated_352_residues
MTDIGKKKATIYDLSMLSGASPSTVSAVLNGSWRKRRIKEATAQLIQKLANEHGYTANLQARGLRSSRSGLVGLLLPVHD
NRYFSSMAQSFEAQVRSRGQCPLVVSACRDPEEERKVVATLISYSIDELFIAGATDPDGVHKVCEKAGLKHVNIDLPGTL
APSIISDNHEGARMLTQAIIERAQEDGPLSPDDLYLFGGRNDHASHERIRGFRDVKRALLGADPDVCIQPTGYSPAMTAR
AFEAFYEGHGKLPRAFFVNSSINLEGLMRFLALHPHETFADIIVGCYDYDPFGSFLPFPVFMIRQDSETMITKGFAILDA
DRRPPITHLVRPTLVPPRTALTGPLDALKDIE
>Mature_351_residues
TDIGKKKATIYDLSMLSGASPSTVSAVLNGSWRKRRIKEATAQLIQKLANEHGYTANLQARGLRSSRSGLVGLLLPVHDN
RYFSSMAQSFEAQVRSRGQCPLVVSACRDPEEERKVVATLISYSIDELFIAGATDPDGVHKVCEKAGLKHVNIDLPGTLA
PSIISDNHEGARMLTQAIIERAQEDGPLSPDDLYLFGGRNDHASHERIRGFRDVKRALLGADPDVCIQPTGYSPAMTARA
FEAFYEGHGKLPRAFFVNSSINLEGLMRFLALHPHETFADIIVGCYDYDPFGSFLPFPVFMIRQDSETMITKGFAILDAD
RRPPITHLVRPTLVPPRTALTGPLDALKDIE

Specific function: Repressor for the genes of the ribitol operon. Binds D- ribulose as an inducer [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786268, Length=321, Percent_Identity=24.2990654205607, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1787906, Length=214, Percent_Identity=25.7009345794392, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 38745; Mature: 38614

Theoretical pI: Translated: 7.10; Mature: 7.10

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDIGKKKATIYDLSMLSGASPSTVSAVLNGSWRKRRIKEATAQLIQKLANEHGYTANLQ
CCCCCCCCEEEEEHHHHCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCEEECH
ARGLRSSRSGLVGLLLPVHDNRYFSSMAQSFEAQVRSRGQCPLVVSACRDPEEERKVVAT
HCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHCCCCCCEEHHCCCCCHHHHHHHHH
LISYSIDELFIAGATDPDGVHKVCEKAGLKHVNIDLPGTLAPSIISDNHEGARMLTQAII
HHHHHHHHEEEECCCCCHHHHHHHHHCCCEEEEECCCCCCCCHHHCCCCHHHHHHHHHHH
ERAQEDGPLSPDDLYLFGGRNDHASHERIRGFRDVKRALLGADPDVCIQPTGYSPAMTAR
HHHHHCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEECCCCCHHHHHH
AFEAFYEGHGKLPRAFFVNSSINLEGLMRFLALHPHETFADIIVGCYDYDPFGSFLPFPV
HHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCE
FMIRQDSETMITKGFAILDADRRPPITHLVRPTLVPPRTALTGPLDALKDIE
EEEECCCCHHHHCCEEEEECCCCCCHHHHCCCCCCCCCCHHCCCHHHHHCCC
>Mature Secondary Structure 
TDIGKKKATIYDLSMLSGASPSTVSAVLNGSWRKRRIKEATAQLIQKLANEHGYTANLQ
CCCCCCCEEEEEHHHHCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCEEECH
ARGLRSSRSGLVGLLLPVHDNRYFSSMAQSFEAQVRSRGQCPLVVSACRDPEEERKVVAT
HCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHCCCCCCEEHHCCCCCHHHHHHHHH
LISYSIDELFIAGATDPDGVHKVCEKAGLKHVNIDLPGTLAPSIISDNHEGARMLTQAII
HHHHHHHHEEEECCCCCHHHHHHHHHCCCEEEEECCCCCCCCHHHCCCCHHHHHHHHHHH
ERAQEDGPLSPDDLYLFGGRNDHASHERIRGFRDVKRALLGADPDVCIQPTGYSPAMTAR
HHHHHCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEECCCCCHHHHHH
AFEAFYEGHGKLPRAFFVNSSINLEGLMRFLALHPHETFADIIVGCYDYDPFGSFLPFPV
HHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCE
FMIRQDSETMITKGFAILDADRRPPITHLVRPTLVPPRTALTGPLDALKDIE
EEEECCCCHHHHCCEEEEECCCCCCHHHHCCCCCCCCCCHHCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3891331 [H]