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The map label for this gene is yafC [H]

Identifier: 116248839

GI number: 116248839

Start: 168852

End: 169754

Strand: Direct

Name: yafC [H]

Synonym: pRL120168

Alternate gene names: 116248839

Gene position: 168852-169754 (Clockwise)

Preceding gene: 116248828

Following gene: 116248845

Centisome position: 19.41

GC content: 55.26

Gene sequence:

>903_bases
ATGGAAGTCTTTGTAATGGCAGCCGAATTGCAGAGCTTTTCCGCCGCTGGACGGCGGCTCAAAATGTCTCCGTCTGCCGT
CAGCAAACTCGTGACGCGCATCGAAGATCGTCTCGGGACACGCCTGCTGGTGCGCTCGACGCGGACAATGACATTAACAC
CGGAGGGTGAAGTATATCTCTCTCGAGCGCGGCGTATCCTGACCGATATAACAGAGACAGAACAGATTGTGGCTGGCGGA
GGCAAAATGATCCCGCGCGGACTGCTTCGCGTTAATGCGTCCGTGGGCTTTGGAGAAAGACACCTCCTTGCACTCATACC
TGCTTTTCTCGAACTCTATCCTGAAGTTCAGCTCGACATTTCGCTGACGGACGGCATCATTGGTCTGATTGAGGAACGCA
CAGATATAGCAATTCGATCTGGAGCAATGGATGACTCGTCCCTAAAGGCGCGCAAGCTTCTCGAAAGCCGACGGGTCATT
GTTGCGTCCCCCGCCTATCTAGACGCAAAGGGCATACCGAAAACTCCACAGGACCTCGCCAACCACAACTGTTTCAGCTT
CAATTTCCGGCGTTCCCTGAACGAATGGCCATTCCGGAACCCTGGGACGTCGGAGGTCTATCGCCTTCCCGTAACGGGTA
ATACCGCGGTCAACAGCGGCATGGTCATGCGCCGACTTTGCCTTTCCGGCAGTGGATTGGGCAGGGTGGGCCAATTCCAT
GTTCAGCCGGATATCGACGCAGGGAAGCTGATCCCGTTGCTAGAGGAATACAATCCTCACGACATGGAACAGATTCATGC
AGTGTTTGCTGGACATGAGCATCTTGCCGCTCGCATTCGCGCGTTCATCGACTTCCTGGCGGAGCACCTCAGTGACGAAG
GAAAATCGGAGCCGCTGCCGTGA

Upstream 100 bases:

>100_bases
AATATGGGTGGAGAGAACTTGAATGATAATCGGGAGTATTGGATAAGTACCTGTGAATTACTTTCATAGGCGAAAGACAA
TGGATAACCGAACAGGGGAA

Downstream 100 bases:

>100_bases
AACCACCATCGAGCGGTTTTCTGACGGCTCGGAAACATCAGCCGGTAGCACTGAGGTAACGATGACGATCGCATCCGAAA
GAGATGATGCGTGACTGGCT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
MEVFVMAAELQSFSAAGRRLKMSPSAVSKLVTRIEDRLGTRLLVRSTRTMTLTPEGEVYLSRARRILTDITETEQIVAGG
GKMIPRGLLRVNASVGFGERHLLALIPAFLELYPEVQLDISLTDGIIGLIEERTDIAIRSGAMDDSSLKARKLLESRRVI
VASPAYLDAKGIPKTPQDLANHNCFSFNFRRSLNEWPFRNPGTSEVYRLPVTGNTAVNSGMVMRRLCLSGSGLGRVGQFH
VQPDIDAGKLIPLLEEYNPHDMEQIHAVFAGHEHLAARIRAFIDFLAEHLSDEGKSEPLP

Sequences:

>Translated_300_residues
MEVFVMAAELQSFSAAGRRLKMSPSAVSKLVTRIEDRLGTRLLVRSTRTMTLTPEGEVYLSRARRILTDITETEQIVAGG
GKMIPRGLLRVNASVGFGERHLLALIPAFLELYPEVQLDISLTDGIIGLIEERTDIAIRSGAMDDSSLKARKLLESRRVI
VASPAYLDAKGIPKTPQDLANHNCFSFNFRRSLNEWPFRNPGTSEVYRLPVTGNTAVNSGMVMRRLCLSGSGLGRVGQFH
VQPDIDAGKLIPLLEEYNPHDMEQIHAVFAGHEHLAARIRAFIDFLAEHLSDEGKSEPLP
>Mature_300_residues
MEVFVMAAELQSFSAAGRRLKMSPSAVSKLVTRIEDRLGTRLLVRSTRTMTLTPEGEVYLSRARRILTDITETEQIVAGG
GKMIPRGLLRVNASVGFGERHLLALIPAFLELYPEVQLDISLTDGIIGLIEERTDIAIRSGAMDDSSLKARKLLESRRVI
VASPAYLDAKGIPKTPQDLANHNCFSFNFRRSLNEWPFRNPGTSEVYRLPVTGNTAVNSGMVMRRLCLSGSGLGRVGQFH
VQPDIDAGKLIPLLEEYNPHDMEQIHAVFAGHEHLAARIRAFIDFLAEHLSDEGKSEPLP

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=292, Percent_Identity=39.3835616438356, Blast_Score=196, Evalue=1e-51,
Organism=Escherichia coli, GI1789639, Length=299, Percent_Identity=33.7792642140468, Blast_Score=145, Evalue=2e-36,
Organism=Escherichia coli, GI87081978, Length=293, Percent_Identity=30.0341296928328, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI145693193, Length=287, Percent_Identity=29.9651567944251, Blast_Score=131, Evalue=4e-32,
Organism=Escherichia coli, GI1789440, Length=292, Percent_Identity=30.1369863013699, Blast_Score=127, Evalue=9e-31,
Organism=Escherichia coli, GI1787589, Length=286, Percent_Identity=30.0699300699301, Blast_Score=110, Evalue=9e-26,
Organism=Escherichia coli, GI1787128, Length=287, Percent_Identity=26.1324041811847, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1789173, Length=252, Percent_Identity=28.5714285714286, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1786448, Length=294, Percent_Identity=23.469387755102, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1788706, Length=201, Percent_Identity=25.8706467661692, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1788481, Length=299, Percent_Identity=23.7458193979933, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1789506, Length=163, Percent_Identity=30.6748466257669, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33200; Mature: 33200

Theoretical pI: Translated: 7.74; Mature: 7.74

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEVFVMAAELQSFSAAGRRLKMSPSAVSKLVTRIEDRLGTRLLVRSTRTMTLTPEGEVYL
CEEEEEHHHHHHHHHCCCEEECCHHHHHHHHHHHHHHHCCEEEEECCCEEEECCCCHHHH
SRARRILTDITETEQIVAGGGKMIPRGLLRVNASVGFGERHLLALIPAFLELYPEVQLDI
HHHHHHHHHHHHHHHHHHCCCCCCCCHHEEEECCCCCCHHHHHHHHHHHHHHCCCEEEEE
SLTDGIIGLIEERTDIAIRSGAMDDSSLKARKLLESRRVIVASPAYLDAKGIPKTPQDLA
EECCCHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHCCEEEECCCEECCCCCCCCHHHHH
NHNCFSFNFRRSLNEWPFRNPGTSEVYRLPVTGNTAVNSGMVMRRLCLSGSGLGRVGQFH
CCCEEEEHHHHCCCCCCCCCCCCCCEEEEECCCCCHHCCHHHHHHHHHCCCCCCCCCEEE
VQPDIDAGKLIPLLEEYNPHDMEQIHAVFAGHEHLAARIRAFIDFLAEHLSDEGKSEPLP
ECCCCCCCCCCHHHHCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MEVFVMAAELQSFSAAGRRLKMSPSAVSKLVTRIEDRLGTRLLVRSTRTMTLTPEGEVYL
CEEEEEHHHHHHHHHCCCEEECCHHHHHHHHHHHHHHHCCEEEEECCCEEEECCCCHHHH
SRARRILTDITETEQIVAGGGKMIPRGLLRVNASVGFGERHLLALIPAFLELYPEVQLDI
HHHHHHHHHHHHHHHHHHCCCCCCCCHHEEEECCCCCCHHHHHHHHHHHHHHCCCEEEEE
SLTDGIIGLIEERTDIAIRSGAMDDSSLKARKLLESRRVIVASPAYLDAKGIPKTPQDLA
EECCCHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHCCEEEECCCEECCCCCCCCHHHHH
NHNCFSFNFRRSLNEWPFRNPGTSEVYRLPVTGNTAVNSGMVMRRLCLSGSGLGRVGQFH
CCCEEEEHHHHCCCCCCCCCCCCCCEEEEECCCCCHHCCHHHHHHHHHCCCCCCCCCEEE
VQPDIDAGKLIPLLEEYNPHDMEQIHAVFAGHEHLAARIRAFIDFLAEHLSDEGKSEPLP
ECCCCCCCCCCHHHHCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]