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The map label for this gene is rffG [C]

Identifier: 116248798

GI number: 116248798

Start: 122972

End: 123973

Strand: Reverse

Name: rffG [C]

Synonym: pRL120124

Alternate gene names: 116248798

Gene position: 123973-122972 (Counterclockwise)

Preceding gene: 116248799

Following gene: 116248797

Centisome position: 14.25

GC content: 61.78

Gene sequence:

>1002_bases
ATGAGCGTCCTTGCACGGGAATGGCTCGAACGCGATGCCAAGGCGCGTGTCGTCATTCTCGATCGTGCCGGCCTGGATGC
GATGGCGGAGCGCTATTTTGCGCCGGTCCGCGATCGGCTGACCGTGGTCACGGCCGACATCTGCGAGCCCACCCAGTGGG
AAGCTGCACTCGACGACCACGATATCCGCTATGTCGTGCACGGCGCCACCGTCACCCCCATCTCGCGTGGCAGCGCGGCT
GAGGCAAAGCGTCAGCCAGAGGCCGAAGATCCGGCCAGGATCATCGATGTCAATTTCATGGGCACGGTGCATGTTCTCGA
ATGGCTGAGAAAACGCCCCGGCTTTCAACGCCTCGTCTTCGTCAGTTCCGGTTCGGTCTACCGTCATCACGGACCGGACT
GGATGGGCGAACCGCTTCCCGAAGACGGCTATGTTGCACCGCTGACACTCTATGGCATCTCCAAATTTGCAGCAGAGATG
GTCGTCAACCGCTACGCAGATCTTTTCGGCCTGTCGGCAGCCTCTGTCCGGCTTGCATCGGTTTATGGGCCGATGGATCG
GGTCACCGGCAGCCGCAATTTCCGGCATGTTCCAAACCGCGTCGCCCATATGGCGCTTGCAGGGGAGACCGTGCGCCCGA
ATAGCCTGGAACCTGTCGGCGACTATGTCACTTCCACGGATGTGGCCGACGCAATCATCGCGCTGCTTCTCGCAGAGCGC
CTCCGTTACCGGCACTACAATATCGGCAGTGGCGAGACTTCGACCATCGGCGACATCATCGGCTGGGCGAAGGAGCGCGC
GCCTTCCCTGCAGGTCGAGGTGAGCCCCGCGGATACCGCCAACGTCATCCAGGACGTTTCACTGAAAGACGGCATGTGGG
GAGCCTACGACATCGCCCGGATCGCGCGCGACACGGGCTGGCAGCCACGCAGCGCCAGGCAGTCGTTCCACGACTACATG
GACTGGATCGTCGAGAACGAAAATCAGAGCGAGGGAAAGTGA

Upstream 100 bases:

>100_bases
CCTGGCCGACGGCGGCTACGCGACGCGCTGATATCTGTATTGAAAATCTTTGGATGGATTTGAAATGACCCTTCTCGTCA
CCGGTGGAACCGGCTTCGTC

Downstream 100 bases:

>100_bases
TGAGCGCTGCTGCAAAAGACCAATCGCGCGATTTTGTCGGCTATGGCCCGAACCCACCGGTCATCTCCTGGCCAGGTGGC
GTCACGCTGGCGATCAACCT

Product: putative NAD-dependent epimerase/dehydratase

Products: NA

Alternate protein names: Galactowaldenase; UDP-galactose 4-epimerase [H]

Number of amino acids: Translated: 333; Mature: 332

Protein sequence:

>333_residues
MSVLAREWLERDAKARVVILDRAGLDAMAERYFAPVRDRLTVVTADICEPTQWEAALDDHDIRYVVHGATVTPISRGSAA
EAKRQPEAEDPARIIDVNFMGTVHVLEWLRKRPGFQRLVFVSSGSVYRHHGPDWMGEPLPEDGYVAPLTLYGISKFAAEM
VVNRYADLFGLSAASVRLASVYGPMDRVTGSRNFRHVPNRVAHMALAGETVRPNSLEPVGDYVTSTDVADAIIALLLAER
LRYRHYNIGSGETSTIGDIIGWAKERAPSLQVEVSPADTANVIQDVSLKDGMWGAYDIARIARDTGWQPRSARQSFHDYM
DWIVENENQSEGK

Sequences:

>Translated_333_residues
MSVLAREWLERDAKARVVILDRAGLDAMAERYFAPVRDRLTVVTADICEPTQWEAALDDHDIRYVVHGATVTPISRGSAA
EAKRQPEAEDPARIIDVNFMGTVHVLEWLRKRPGFQRLVFVSSGSVYRHHGPDWMGEPLPEDGYVAPLTLYGISKFAAEM
VVNRYADLFGLSAASVRLASVYGPMDRVTGSRNFRHVPNRVAHMALAGETVRPNSLEPVGDYVTSTDVADAIIALLLAER
LRYRHYNIGSGETSTIGDIIGWAKERAPSLQVEVSPADTANVIQDVSLKDGMWGAYDIARIARDTGWQPRSARQSFHDYM
DWIVENENQSEGK
>Mature_332_residues
SVLAREWLERDAKARVVILDRAGLDAMAERYFAPVRDRLTVVTADICEPTQWEAALDDHDIRYVVHGATVTPISRGSAAE
AKRQPEAEDPARIIDVNFMGTVHVLEWLRKRPGFQRLVFVSSGSVYRHHGPDWMGEPLPEDGYVAPLTLYGISKFAAEMV
VNRYADLFGLSAASVRLASVYGPMDRVTGSRNFRHVPNRVAHMALAGETVRPNSLEPVGDYVTSTDVADAIIALLLAERL
RYRHYNIGSGETSTIGDIIGWAKERAPSLQVEVSPADTANVIQDVSLKDGMWGAYDIARIARDTGWQPRSARQSFHDYMD
WIVENENQSEGK

Specific function: INVOLVED IN THE SYNTHESIS OF ENTEROBACTERIAL COMMON ANTIGEN (ECA) AND REQUIRED FOR SYNTHESIS OF LIPOPOLYSACCHARIDE O-SIDE CHAINS. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =5.1.3.2 [H]

Molecular weight: Translated: 37093; Mature: 36962

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVLAREWLERDAKARVVILDRAGLDAMAERYFAPVRDRLTVVTADICEPTQWEAALDDH
CCHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHCEEEEEECCCCCCCCHHCCCCC
DIRYVVHGATVTPISRGSAAEAKRQPEAEDPARIIDVNFMGTVHVLEWLRKRPGFQRLVF
CEEEEEECCEECCCCCCCCHHHHCCCCCCCCCEEEEEECCHHHHHHHHHHHCCCCEEEEE
VSSGSVYRHHGPDWMGEPLPEDGYVAPLTLYGISKFAAEMVVNRYADLFGLSAASVRLAS
EECCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
VYGPMDRVTGSRNFRHVPNRVAHMALAGETVRPNSLEPVGDYVTSTDVADAIIALLLAER
HHCCHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LRYRHYNIGSGETSTIGDIIGWAKERAPSLQVEVSPADTANVIQDVSLKDGMWGAYDIAR
HHHHCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHCCCCCCCCCHHHHHH
IARDTGWQPRSARQSFHDYMDWIVENENQSEGK
HHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
SVLAREWLERDAKARVVILDRAGLDAMAERYFAPVRDRLTVVTADICEPTQWEAALDDH
CHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHCEEEEEECCCCCCCCHHCCCCC
DIRYVVHGATVTPISRGSAAEAKRQPEAEDPARIIDVNFMGTVHVLEWLRKRPGFQRLVF
CEEEEEECCEECCCCCCCCHHHHCCCCCCCCCEEEEEECCHHHHHHHHHHHCCCCEEEEE
VSSGSVYRHHGPDWMGEPLPEDGYVAPLTLYGISKFAAEMVVNRYADLFGLSAASVRLAS
EECCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
VYGPMDRVTGSRNFRHVPNRVAHMALAGETVRPNSLEPVGDYVTSTDVADAIIALLLAER
HHCCHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LRYRHYNIGSGETSTIGDIIGWAKERAPSLQVEVSPADTANVIQDVSLKDGMWGAYDIAR
HHHHCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHCCCCCCCCCHHHHHH
IARDTGWQPRSARQSFHDYMDWIVENENQSEGK
HHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]