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The map label for this gene is ilvB [H]

Identifier: 116248796

GI number: 116248796

Start: 120380

End: 122071

Strand: Reverse

Name: ilvB [H]

Synonym: pRL120122

Alternate gene names: 116248796

Gene position: 122071-120380 (Counterclockwise)

Preceding gene: 116248797

Following gene: 116248795

Centisome position: 14.03

GC content: 62.29

Gene sequence:

>1692_bases
ATGGCTGACGGCACGCATGCACTGCCGAATGGCCATTTGCCCGCCGAGGCGAAAACGATGAGCGGCGCCGAATATGTTGC
AGGGGTCCTGAAGGAGGAAGGCGTCAAGCAGATCATTGGCTTTCCAGCAAGCGAGCTTTTCGATGCCTCAGCGGCGCTCG
ACATTCCGCCGCTGATTGCGCGAACGCAGCGTGTCGCCGTCAATATCGCAGAGGGCTATGCCCGGGCGACGGCCGGCAGG
GAGCTTGCCGTTGTCACTGTGCAATATGGCCCGGGAGCCGAAAGTGCATTTGCCGGCATTGCCCAGGCTTTCAACGACCG
CGTACCGATGCTGTTCCTGCCGACCGGCTACCCGCGCGGATCAGAAGCCATCGCCCCCAATTTCGAATCTCGCCGCAACT
TTCGGCACATTACCAAATGGTGCGAGGCGGCAACGCAGGTCGATGATCTGCCCAAGCTCATGCATGCGGCGATATCGCGG
GTCAGGAATGGGCCGCCGGCGCCGGTCCTGCTCGAGTTGCCTGTCGATCTGCTGGCCGAAAGGCTTGATGCTCTGCCAGC
GGATCATCGCCGACCGCGCCGCAGCGTACCGCAGGCCGGTGATCGTGAGGTGGTCGATCTGGCCGAAAGCCTGCTGGCCG
CCAAAAATCCCGTCATTCTCGCGGGGCAGGGAGTGCTGTCAGCCTCGGCGACGCCGGAACTGATCGAGCTTGCCGAACTC
TTGAACATTCCCGTCATGACGACGCCGAACGGCAAGAGCGCTTTTCCGGAAGACCATCCGCTTGCTCTCGGGACAGCCGG
GCGTGCCCGACCCGCGACCGTCGACCATTTTCTAGCAAAGGCCGACCTGGTGTTCGCGCTCGGCAGCAGCCTGACGCGCT
CCTACTACATCCTGCCGGTTCCCGAAGATAAAACCATCCTGCATGTCACGCTCGACGATGCGGATCTCGGCAAGGATTAT
GCGATTGCCCAGGGTGTGGTCGCAGACGCCGCCTCGGTCCTGAAACAGATGATCGCACTCATCCGTCGCGTTCACCTGCC
TCTCGGTGAACGTCAGCAGCCGGCGGCGGAAATCGCCGCCGTTCGCGCGGCTTTCATGGAAAAATGGATGCCGCTCCTGA
CATCGGACAGCACGCCGATCTCACCTTACCGTGTCGTCTGGGAGATCATGCAGGCGATCGATCCGAAGACCTCAATCGTG
ACGCACGACGCCGGCAATCCGCGCGACCAGTTCTGCCCATTTTATTTGGCAAGCACACCCGGCAGCTACATCGGTTGGGG
CAAGACCACCCAGCTCGGAAGCGGCCTCGGCTTTGCGATGGGGGCCAGGCTTGCTCGTCCCGATGCGACCGTCGTCAATC
TGATGGGAGAGTCCGCGTTCGGAATGGTCGGCATCGACTTCGAAACCGCAGTCCGCTGCAATCTGCCTGTCTTGACCATC
GTGCTGCGCAACAACGTGCTCGGCGGCTACGCGAAAAGCATGCCGATTGCCACCACGAAATACGGCGCGAATCGGGTGAC
AGGCAGTTACGCGCCGATCGCGGAAGCTCTCGGTGGTTACGCGGAATGCGTCGTGGACCCGGAAATGCTGCGCCCCGCGT
TGCTACGTGCCCTCGACAAGGTCAAGGCCGGGCAGGCGGCCCTTATCGAGGTCGTCACGCACGAAGAGTCGCGGCTCGCG
GGTCTATGGTAG

Upstream 100 bases:

>100_bases
CGGGCGTCGGGGCTGCGTGACGTGATCGAGCACGTCAAGAAAACGCCAGGTGCGGCCTTCATGCGGCGCGAGGATATCGC
CCGTTACTGGATCGATCATC

Downstream 100 bases:

>100_bases
GGGAGGCGAAGGCGACTATGTTCGACACGCTGATCACCGGCGGCATGGTCGTCAACGCAAGCGGTCAACTTCCTGCGGAT
ATCGGCATTCTCGACGGCGT

Product: hypothetical protein

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS [H]

Number of amino acids: Translated: 563; Mature: 562

Protein sequence:

>563_residues
MADGTHALPNGHLPAEAKTMSGAEYVAGVLKEEGVKQIIGFPASELFDASAALDIPPLIARTQRVAVNIAEGYARATAGR
ELAVVTVQYGPGAESAFAGIAQAFNDRVPMLFLPTGYPRGSEAIAPNFESRRNFRHITKWCEAATQVDDLPKLMHAAISR
VRNGPPAPVLLELPVDLLAERLDALPADHRRPRRSVPQAGDREVVDLAESLLAAKNPVILAGQGVLSASATPELIELAEL
LNIPVMTTPNGKSAFPEDHPLALGTAGRARPATVDHFLAKADLVFALGSSLTRSYYILPVPEDKTILHVTLDDADLGKDY
AIAQGVVADAASVLKQMIALIRRVHLPLGERQQPAAEIAAVRAAFMEKWMPLLTSDSTPISPYRVVWEIMQAIDPKTSIV
THDAGNPRDQFCPFYLASTPGSYIGWGKTTQLGSGLGFAMGARLARPDATVVNLMGESAFGMVGIDFETAVRCNLPVLTI
VLRNNVLGGYAKSMPIATTKYGANRVTGSYAPIAEALGGYAECVVDPEMLRPALLRALDKVKAGQAALIEVVTHEESRLA
GLW

Sequences:

>Translated_563_residues
MADGTHALPNGHLPAEAKTMSGAEYVAGVLKEEGVKQIIGFPASELFDASAALDIPPLIARTQRVAVNIAEGYARATAGR
ELAVVTVQYGPGAESAFAGIAQAFNDRVPMLFLPTGYPRGSEAIAPNFESRRNFRHITKWCEAATQVDDLPKLMHAAISR
VRNGPPAPVLLELPVDLLAERLDALPADHRRPRRSVPQAGDREVVDLAESLLAAKNPVILAGQGVLSASATPELIELAEL
LNIPVMTTPNGKSAFPEDHPLALGTAGRARPATVDHFLAKADLVFALGSSLTRSYYILPVPEDKTILHVTLDDADLGKDY
AIAQGVVADAASVLKQMIALIRRVHLPLGERQQPAAEIAAVRAAFMEKWMPLLTSDSTPISPYRVVWEIMQAIDPKTSIV
THDAGNPRDQFCPFYLASTPGSYIGWGKTTQLGSGLGFAMGARLARPDATVVNLMGESAFGMVGIDFETAVRCNLPVLTI
VLRNNVLGGYAKSMPIATTKYGANRVTGSYAPIAEALGGYAECVVDPEMLRPALLRALDKVKAGQAALIEVVTHEESRLA
GLW
>Mature_562_residues
ADGTHALPNGHLPAEAKTMSGAEYVAGVLKEEGVKQIIGFPASELFDASAALDIPPLIARTQRVAVNIAEGYARATAGRE
LAVVTVQYGPGAESAFAGIAQAFNDRVPMLFLPTGYPRGSEAIAPNFESRRNFRHITKWCEAATQVDDLPKLMHAAISRV
RNGPPAPVLLELPVDLLAERLDALPADHRRPRRSVPQAGDREVVDLAESLLAAKNPVILAGQGVLSASATPELIELAELL
NIPVMTTPNGKSAFPEDHPLALGTAGRARPATVDHFLAKADLVFALGSSLTRSYYILPVPEDKTILHVTLDDADLGKDYA
IAQGVVADAASVLKQMIALIRRVHLPLGERQQPAAEIAAVRAAFMEKWMPLLTSDSTPISPYRVVWEIMQAIDPKTSIVT
HDAGNPRDQFCPFYLASTPGSYIGWGKTTQLGSGLGFAMGARLARPDATVVNLMGESAFGMVGIDFETAVRCNLPVLTIV
LRNNVLGGYAKSMPIATTKYGANRVTGSYAPIAEALGGYAECVVDPEMLRPALLRALDKVKAGQAALIEVVTHEESRLAG
LW

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=551, Percent_Identity=24.8638838475499, Blast_Score=129, Evalue=5e-30,
Organism=Homo sapiens, GI21361361, Length=519, Percent_Identity=25.2408477842004, Blast_Score=112, Evalue=1e-24,
Organism=Escherichia coli, GI87081685, Length=556, Percent_Identity=29.136690647482, Blast_Score=198, Evalue=6e-52,
Organism=Escherichia coli, GI1790104, Length=483, Percent_Identity=28.1573498964803, Blast_Score=149, Evalue=4e-37,
Organism=Escherichia coli, GI1786717, Length=561, Percent_Identity=25.3119429590018, Blast_Score=139, Evalue=5e-34,
Organism=Escherichia coli, GI1787096, Length=511, Percent_Identity=25.6360078277886, Blast_Score=105, Evalue=1e-23,
Organism=Escherichia coli, GI1788716, Length=471, Percent_Identity=25.0530785562633, Blast_Score=81, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17542570, Length=605, Percent_Identity=22.4793388429752, Blast_Score=97, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17531299, Length=557, Percent_Identity=24.0574506283662, Blast_Score=95, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI17531301, Length=557, Percent_Identity=24.0574506283662, Blast_Score=94, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6323755, Length=532, Percent_Identity=25, Blast_Score=142, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6320816, Length=499, Percent_Identity=21.2424849699399, Blast_Score=90, Evalue=1e-18,
Organism=Drosophila melanogaster, GI19922626, Length=497, Percent_Identity=25.1509054325956, Blast_Score=113, Evalue=3e-25,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 60088; Mature: 59957

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADGTHALPNGHLPAEAKTMSGAEYVAGVLKEEGVKQIIGFPASELFDASAALDIPPLIA
CCCCCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCHHHH
RTQRVAVNIAEGYARATAGRELAVVTVQYGPGAESAFAGIAQAFNDRVPMLFLPTGYPRG
HHHHHEEEHHHHHHHHCCCCEEEEEEEEECCCCHHHHHHHHHHHCCCCCEEEEECCCCCC
SEAIAPNFESRRNFRHITKWCEAATQVDDLPKLMHAAISRVRNGPPAPVLLELPVDLLAE
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHH
RLDALPADHRRPRRSVPQAGDREVVDLAESLLAAKNPVILAGQGVLSASATPELIELAEL
HHHCCCCCCCCCHHCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHH
LNIPVMTTPNGKSAFPEDHPLALGTAGRARPATVDHFLAKADLVFALGSSLTRSYYILPV
HCCCEEECCCCCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEC
PEDKTILHVTLDDADLGKDYAIAQGVVADAASVLKQMIALIRRVHLPLGERQQPAAEIAA
CCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
VRAAFMEKWMPLLTSDSTPISPYRVVWEIMQAIDPKTSIVTHDAGNPRDQFCPFYLASTP
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCHHHCCCEEEECCC
GSYIGWGKTTQLGSGLGFAMGARLARPDATVVNLMGESAFGMVGIDFETAVRCNLPVLTI
CCCCCCCCCHHCCCCCCHHHHCCCCCCCHHHHHHHCCCCCCEEEECHHHHEECCCCEEEE
VLRNNVLGGYAKSMPIATTKYGANRVTGSYAPIAEALGGYAECVVDPEMLRPALLRALDK
EECCCCCCCHHHCCCCEEECCCCCCCCCCHHHHHHHHCCHHHEEECHHHHHHHHHHHHHH
VKAGQAALIEVVTHEESRLAGLW
HCCCCHHEEHHHHCCCHHHCCCC
>Mature Secondary Structure 
ADGTHALPNGHLPAEAKTMSGAEYVAGVLKEEGVKQIIGFPASELFDASAALDIPPLIA
CCCCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCHHHH
RTQRVAVNIAEGYARATAGRELAVVTVQYGPGAESAFAGIAQAFNDRVPMLFLPTGYPRG
HHHHHEEEHHHHHHHHCCCCEEEEEEEEECCCCHHHHHHHHHHHCCCCCEEEEECCCCCC
SEAIAPNFESRRNFRHITKWCEAATQVDDLPKLMHAAISRVRNGPPAPVLLELPVDLLAE
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHH
RLDALPADHRRPRRSVPQAGDREVVDLAESLLAAKNPVILAGQGVLSASATPELIELAEL
HHHCCCCCCCCCHHCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHH
LNIPVMTTPNGKSAFPEDHPLALGTAGRARPATVDHFLAKADLVFALGSSLTRSYYILPV
HCCCEEECCCCCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEC
PEDKTILHVTLDDADLGKDYAIAQGVVADAASVLKQMIALIRRVHLPLGERQQPAAEIAA
CCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
VRAAFMEKWMPLLTSDSTPISPYRVVWEIMQAIDPKTSIVTHDAGNPRDQFCPFYLASTP
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCHHHCCCEEEECCC
GSYIGWGKTTQLGSGLGFAMGARLARPDATVVNLMGESAFGMVGIDFETAVRCNLPVLTI
CCCCCCCCCHHCCCCCCHHHHCCCCCCCHHHHHHHCCCCCCEEEECHHHHEECCCCEEEE
VLRNNVLGGYAKSMPIATTKYGANRVTGSYAPIAEALGGYAECVVDPEMLRPALLRALDK
EECCCCCCCHHHCCCCEEECCCCCCCCCCHHHHHHHHCCHHHEEECHHHHHHHHHHHHHH
VKAGQAALIEVVTHEESRLAGLW
HCCCCHHEEHHHHCCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]