BacMap

Definition	Rhizobium leguminosarum bv. viciae 3841 plasmid pRL12, complete sequence.
Accession	NC_008378
Length	870,021

Click here to switch to the map view.

The map label for this gene is yohF [C]

Identifier: 116248794

GI number: 116248794

Start: 118244

End: 119017

Strand: Reverse

Name: yohF [C]

Synonym: pRL120120

Alternate gene names: 116248794

Gene position: 119017-118244 (Counterclockwise)

Preceding gene: 116248795

Following gene: 116248793

Centisome position: 13.68

GC content: 60.34

Gene sequence:

>774_bases
ATGCCTTTGCTCGACAGGAAAATTGCCATTGTCACCGGCGCGAGCTCTGGCCTCGGCCGCGCGATCGCACTTCGCTACGC
CGAAGAGGGTGCGGTCGTCGTGATCGCCGACGTCTCCGAGGCTCCGATCGAGGGGGGTGAAACGACCCTTTCCTGCATCG
TTGCGGCAGGTGGAAAGGGCATGTTCGTAAGGACCGACATCTCCAACTGGGCTGACGTCGATGCGCTGGTCTCACAAACG
GTGAAAGAGTTCGGTCGGCTCGACATCATGGTCAACAATGCCGCGATCTACACCTCGACCAATCTCCTTGAAACCTCGCC
CCAACAATGGGATAGGGTGATCGCCGTCAATGTCACCGGGTTCTTTTATTGTTGCAAGCGAGCCGCGCAGCAATTTCTGG
CGCAGGAGCCGACCGGTGAAACGCGTGGCCGCATCATCAACATTTCATCGCAGCACGGCATGGTCGCTTGCCCTGGTGAT
TTCCCATACGGCGTCAGCAAGGGTGCAGTCGTGCAAATGACCCGGCAAATTGCCGTCGACCATGCTGCGACAGGTATCGT
TTGCAATGCGATCGCCCCCGGCAAGATCGTCACCGGCAAACCGGGTGTCGCCAACGATCCCGACGCTCTCGATTATTCGC
GCCGTCGCACCCCTTGGCCGCGGCTCGGCGAGCCACACGATGTCGCAAGTGCCGCGCTTTTCCTCGCCAGCGACATGGCG
AGCTACATGACCGGCGTTAACCTGATGGTCGATGGCGGCTGGATGGCGGGCTGA

Upstream 100 bases:

>100_bases
ACCGACGTCTTCCTGCGCGGCGCACGCATCGTCAAAGACGGAACGCTTATTAATCCCCGTCGCGGCCAAGTCGTCCGCAG
GACTGCATAAAGGAACGCCG

Downstream 100 bases:

>100_bases
TGATCACAGCGTATGAAGATCGCCAATAACGGAAAGGAAAACGGACCATGGATCTCGGATTGAAAGGCAAGACTGCCCTG
GTATTGGGCGCCGGCGGCGG

Product: putative short-chain dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 257; Mature: 256

Protein sequence:

>257_residues
MPLLDRKIAIVTGASSGLGRAIALRYAEEGAVVVIADVSEAPIEGGETTLSCIVAAGGKGMFVRTDISNWADVDALVSQT
VKEFGRLDIMVNNAAIYTSTNLLETSPQQWDRVIAVNVTGFFYCCKRAAQQFLAQEPTGETRGRIINISSQHGMVACPGD
FPYGVSKGAVVQMTRQIAVDHAATGIVCNAIAPGKIVTGKPGVANDPDALDYSRRRTPWPRLGEPHDVASAALFLASDMA
SYMTGVNLMVDGGWMAG

Sequences:

>Translated_257_residues
MPLLDRKIAIVTGASSGLGRAIALRYAEEGAVVVIADVSEAPIEGGETTLSCIVAAGGKGMFVRTDISNWADVDALVSQT
VKEFGRLDIMVNNAAIYTSTNLLETSPQQWDRVIAVNVTGFFYCCKRAAQQFLAQEPTGETRGRIINISSQHGMVACPGD
FPYGVSKGAVVQMTRQIAVDHAATGIVCNAIAPGKIVTGKPGVANDPDALDYSRRRTPWPRLGEPHDVASAALFLASDMA
SYMTGVNLMVDGGWMAG
>Mature_256_residues
PLLDRKIAIVTGASSGLGRAIALRYAEEGAVVVIADVSEAPIEGGETTLSCIVAAGGKGMFVRTDISNWADVDALVSQTV
KEFGRLDIMVNNAAIYTSTNLLETSPQQWDRVIAVNVTGFFYCCKRAAQQFLAQEPTGETRGRIINISSQHGMVACPGDF
PYGVSKGAVVQMTRQIAVDHAATGIVCNAIAPGKIVTGKPGVANDPDALDYSRRRTPWPRLGEPHDVASAALFLASDMAS
YMTGVNLMVDGGWMAG

Specific function: Unknown

COG id: COG1028

COG function: function code IQR; Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI15277342, Length=261, Percent_Identity=34.8659003831418, Blast_Score=120, Evalue=1e-27,
Organism=Homo sapiens, GI19923817, Length=261, Percent_Identity=30.6513409961686, Blast_Score=112, Evalue=2e-25,
Organism=Homo sapiens, GI59889578, Length=234, Percent_Identity=34.6153846153846, Blast_Score=111, Evalue=8e-25,
Organism=Homo sapiens, GI32483357, Length=257, Percent_Identity=32.295719844358, Blast_Score=108, Evalue=7e-24,
Organism=Homo sapiens, GI4758504, Length=254, Percent_Identity=31.8897637795276, Blast_Score=97, Evalue=1e-20,
Organism=Homo sapiens, GI10190704, Length=257, Percent_Identity=32.295719844358, Blast_Score=97, Evalue=1e-20,
Organism=Homo sapiens, GI66933014, Length=263, Percent_Identity=29.6577946768061, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI40254992, Length=249, Percent_Identity=29.718875502008, Blast_Score=91, Evalue=8e-19,
Organism=Homo sapiens, GI5031737, Length=261, Percent_Identity=31.4176245210728, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI126723750, Length=260, Percent_Identity=29.2307692307692, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI4504505, Length=202, Percent_Identity=31.6831683168317, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI83715985, Length=254, Percent_Identity=29.9212598425197, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI7705925, Length=248, Percent_Identity=27.4193548387097, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI40254986, Length=144, Percent_Identity=34.7222222222222, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI31542939, Length=199, Percent_Identity=29.6482412060301, Blast_Score=75, Evalue=8e-14,
Organism=Homo sapiens, GI109715829, Length=249, Percent_Identity=29.3172690763052, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI310128830, Length=154, Percent_Identity=33.7662337662338, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI310115468, Length=154, Percent_Identity=33.7662337662338, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI142976729, Length=196, Percent_Identity=29.0816326530612, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI209862901, Length=221, Percent_Identity=30.316742081448, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI19923983, Length=221, Percent_Identity=30.316742081448, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI126723191, Length=194, Percent_Identity=29.8969072164948, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI40807363, Length=197, Percent_Identity=29.4416243654822, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI210032110, Length=196, Percent_Identity=27.5510204081633, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI33667109, Length=206, Percent_Identity=31.5533980582524, Blast_Score=71, Evalue=8e-13,
Organism=Homo sapiens, GI224922801, Length=177, Percent_Identity=29.3785310734463, Blast_Score=66, Evalue=3e-11,
Organism=Escherichia coli, GI1788459, Length=249, Percent_Identity=33.3333333333333, Blast_Score=135, Evalue=2e-33,
Organism=Escherichia coli, GI2367175, Length=248, Percent_Identity=33.8709677419355, Blast_Score=134, Evalue=4e-33,
Organism=Escherichia coli, GI1789208, Length=263, Percent_Identity=34.2205323193916, Blast_Score=132, Evalue=3e-32,
Organism=Escherichia coli, GI1787335, Length=248, Percent_Identity=33.8709677419355, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI87082100, Length=261, Percent_Identity=33.3333333333333, Blast_Score=127, Evalue=7e-31,
Organism=Escherichia coli, GI1790717, Length=250, Percent_Identity=30, Blast_Score=116, Evalue=1e-27,
Organism=Escherichia coli, GI1787905, Length=250, Percent_Identity=32.8, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI87082160, Length=253, Percent_Identity=31.2252964426877, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1789378, Length=259, Percent_Identity=31.6602316602317, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1786812, Length=252, Percent_Identity=28.5714285714286, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI2367365, Length=253, Percent_Identity=30.8300395256917, Blast_Score=81, Evalue=8e-17,
Organism=Escherichia coli, GI1789057, Length=269, Percent_Identity=28.2527881040892, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1787820, Length=191, Percent_Identity=24.6073298429319, Blast_Score=62, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI25147288, Length=253, Percent_Identity=35.1778656126482, Blast_Score=123, Evalue=8e-29,
Organism=Caenorhabditis elegans, GI17559104, Length=271, Percent_Identity=36.1623616236162, Blast_Score=122, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17555706, Length=254, Percent_Identity=34.6456692913386, Blast_Score=119, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17538480, Length=262, Percent_Identity=31.6793893129771, Blast_Score=115, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17562906, Length=270, Percent_Identity=30.7407407407407, Blast_Score=112, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17561402, Length=261, Percent_Identity=34.0996168582375, Blast_Score=111, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI17531453, Length=274, Percent_Identity=32.1167883211679, Blast_Score=108, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17551412, Length=272, Percent_Identity=32.3529411764706, Blast_Score=108, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17562904, Length=273, Percent_Identity=29.3040293040293, Blast_Score=108, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17562908, Length=264, Percent_Identity=31.0606060606061, Blast_Score=108, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI71994600, Length=254, Percent_Identity=30.3149606299213, Blast_Score=106, Evalue=9e-24,
Organism=Caenorhabditis elegans, GI71994604, Length=256, Percent_Identity=32.03125, Blast_Score=106, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI193204405, Length=270, Percent_Identity=29.2592592592593, Blast_Score=104, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI17560150, Length=271, Percent_Identity=28.7822878228782, Blast_Score=100, Evalue=8e-22,
Organism=Caenorhabditis elegans, GI17562990, Length=270, Percent_Identity=29.6296296296296, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17560676, Length=259, Percent_Identity=29.3436293436293, Blast_Score=97, Evalue=9e-21,
Organism=Caenorhabditis elegans, GI17564282, Length=196, Percent_Identity=31.6326530612245, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI115534694, Length=257, Percent_Identity=33.852140077821, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17562910, Length=273, Percent_Identity=29.6703296703297, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17565030, Length=266, Percent_Identity=30.8270676691729, Blast_Score=95, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI72000259, Length=265, Percent_Identity=27.1698113207547, Blast_Score=94, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI17560332, Length=274, Percent_Identity=29.5620437956204, Blast_Score=94, Evalue=9e-20,
Organism=Caenorhabditis elegans, GI17536025, Length=255, Percent_Identity=30.1960784313725, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17538486, Length=269, Percent_Identity=30.1115241635688, Blast_Score=93, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17560220, Length=270, Percent_Identity=27.4074074074074, Blast_Score=91, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI17538182, Length=254, Percent_Identity=30.3149606299213, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17563726, Length=259, Percent_Identity=27.4131274131274, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17536651, Length=254, Percent_Identity=29.9212598425197, Blast_Score=88, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17568967, Length=199, Percent_Identity=29.6482412060301, Blast_Score=88, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17555612, Length=207, Percent_Identity=34.2995169082126, Blast_Score=87, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17544670, Length=265, Percent_Identity=28.3018867924528, Blast_Score=87, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17561272, Length=260, Percent_Identity=31.1538461538462, Blast_Score=84, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI71997099, Length=213, Percent_Identity=29.1079812206573, Blast_Score=82, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI193203117, Length=220, Percent_Identity=28.1818181818182, Blast_Score=78, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI193203115, Length=220, Percent_Identity=28.1818181818182, Blast_Score=77, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI17508651, Length=256, Percent_Identity=29.6875, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17567345, Length=220, Percent_Identity=33.6363636363636, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17508895, Length=168, Percent_Identity=28.5714285714286, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI71982365, Length=124, Percent_Identity=35.4838709677419, Blast_Score=70, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI86565303, Length=213, Percent_Identity=29.1079812206573, Blast_Score=67, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6324126, Length=198, Percent_Identity=27.7777777777778, Blast_Score=82, Evalue=7e-17,
Organism=Saccharomyces cerevisiae, GI6322861, Length=197, Percent_Identity=29.4416243654822, Blast_Score=82, Evalue=1e-16,
Organism=Drosophila melanogaster, GI21357041, Length=263, Percent_Identity=35.7414448669202, Blast_Score=146, Evalue=1e-35,
Organism=Drosophila melanogaster, GI28571526, Length=261, Percent_Identity=36.0153256704981, Blast_Score=145, Evalue=3e-35,
Organism=Drosophila melanogaster, GI24644339, Length=255, Percent_Identity=33.7254901960784, Blast_Score=140, Evalue=9e-34,
Organism=Drosophila melanogaster, GI23397609, Length=255, Percent_Identity=34.5098039215686, Blast_Score=135, Evalue=3e-32,
Organism=Drosophila melanogaster, GI21355319, Length=259, Percent_Identity=31.2741312741313, Blast_Score=108, Evalue=5e-24,
Organism=Drosophila melanogaster, GI24644337, Length=253, Percent_Identity=32.8063241106719, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI17737361, Length=236, Percent_Identity=34.3220338983051, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI18859837, Length=195, Percent_Identity=33.3333333333333, Blast_Score=102, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24639444, Length=254, Percent_Identity=31.1023622047244, Blast_Score=101, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24649181, Length=169, Percent_Identity=29.585798816568, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI281364892, Length=168, Percent_Identity=30.952380952381, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24643142, Length=251, Percent_Identity=26.2948207171315, Blast_Score=68, Evalue=5e-12,
Organism=Drosophila melanogaster, GI21357673, Length=215, Percent_Identity=28.8372093023256, Blast_Score=68, Evalue=5e-12,
Organism=Drosophila melanogaster, GI21355631, Length=161, Percent_Identity=27.3291925465839, Blast_Score=64, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 27179; Mature: 27048

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLLDRKIAIVTGASSGLGRAIALRYAEEGAVVVIADVSEAPIEGGETTLSCIVAAGGKG
CCCCCCEEEEEECCCCCCCCEEEEEEECCCCEEEEEECCCCCCCCCCCEEEEEEEECCCC
MFVRTDISNWADVDALVSQTVKEFGRLDIMVNNAAIYTSTNLLETSPQQWDRVIAVNVTG
EEEEECCCCCHHHHHHHHHHHHHHCCEEEEECCEEEEEECCCCCCCHHHHCEEEEEECHH
FFYCCKRAAQQFLAQEPTGETRGRIINISSQHGMVACPGDFPYGVSKGAVVQMTRQIAVD
HHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCEEECCCCCCCCCCCCHHHHHHHHHHHH
HAATGIVCNAIAPGKIVTGKPGVANDPDALDYSRRRTPWPRLGEPHDVASAALFLASDMA
HHCCCEEEECCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
SYMTGVNLMVDGGWMAG
HHHCCCEEEEECCCCCC
>Mature Secondary Structure 
PLLDRKIAIVTGASSGLGRAIALRYAEEGAVVVIADVSEAPIEGGETTLSCIVAAGGKG
CCCCCEEEEEECCCCCCCCEEEEEEECCCCEEEEEECCCCCCCCCCCEEEEEEEECCCC
MFVRTDISNWADVDALVSQTVKEFGRLDIMVNNAAIYTSTNLLETSPQQWDRVIAVNVTG
EEEEECCCCCHHHHHHHHHHHHHHCCEEEEECCEEEEEECCCCCCCHHHHCEEEEEECHH
FFYCCKRAAQQFLAQEPTGETRGRIINISSQHGMVACPGDFPYGVSKGAVVQMTRQIAVD
HHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCEEECCCCCCCCCCCCHHHHHHHHHHHH
HAATGIVCNAIAPGKIVTGKPGVANDPDALDYSRRRTPWPRLGEPHDVASAALFLASDMA
HHCCCEEEECCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
SYMTGVNLMVDGGWMAG
HHHCCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]