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The map label for this gene is livM [H]

Identifier: 116248771

GI number: 116248771

Start: 91199

End: 92284

Strand: Reverse

Name: livM [H]

Synonym: pRL120095

Alternate gene names: 116248771

Gene position: 92284-91199 (Counterclockwise)

Preceding gene: 116248772

Following gene: 116248770

Centisome position: 10.61

GC content: 63.35

Gene sequence:

>1086_bases
ATGACCATGGCCATTCCATCCTTCACCGTCGAGACGCGTACCAGGGCATCGACATTGTCGGCTGTCGCGGTCGTTGCCCT
TCTGCTGCTTGCCTTCGCTGCACCCTTCATCGTCGCGCGCGGCGTCATCCAGGACCTTTTCTTCATCCTCACCATGCTGG
TGCTGGCACAGAACTGGAACCTGCTTGCCGGTTATGCCGGGCTGATCTCGGTCGGCCAGCAGGTTTTCGTCGGCTTTGGA
GCCTATACGATGTTCGGCTGCGTCATCCTGTTCGGCGTCGATCCTGTCGTGGCCATCCTGATTGCCGGCGTATTTTCCGT
CGCCCTTGCCATTCCCACAGCCTTCTTCACCTTCCGCCTCTACGGTCCCTATTTCGCCATCGGCACCTGGGTTGTGGCCG
AAGTCGTGCGGTTGCTGCTGGCCCAGTGGAAGGCGCTCGGCGGCGGCACCGGCACCTCGTTGCCGCGGGAGGCGACCCGC
GACATGATGGGCGTCGGCGCGATGCGCGACCTCTTCGGCATGAAGGCTTCCGAGGCGGGCGACGCGCTGACTTACTGGCT
GGCGCTGATCCTCGCCGTCGCCACAATCGGCTTCATCTATGGTCTTCTGCGCAGCAAGCAGGGTCTCGGCCTTGCCGCCG
TTCGTGACAACGAGCAGGCAGCCCGCGCCCTCGGCGTCGATGCGCGGCGGATGAAGACGCTGGTCTATCTCGCCACCGCC
TTCATGACAGGCATCGCCGGGGCGCTGATCTACGTGCAGAAGGCGCGCATCTCGCCGGACGCCGCCTTCTCGGTCACCGA
CTGGACGGCCTATGTCATCTTCATCGTCGTGATCGGCGGCATCGGCACGATCGAGGGACCGATCCTCGGCGTCATCATCT
TCTTTCTCTTACAGAACCTCCTGGCCGATTACGGCTCCTGGTATCTGCTGATGCTCGGTCTTCTCGGCATCGCCATCATG
CTTCTCGCGCCGCGCGGCCTCTGGGGATTGTTCTCCGAGCGCACCGGCATCCAGCTTTTCCCGGTTCGCCGCGTCCTGAA
GGGCGGGCCGCTCAATCTCACGCATAGGGGAGGGACTCATGGCTGA

Upstream 100 bases:

>100_bases
TCCGGAATGGCAGATCCTTGCCGGCCACCTGGCATTCCTCGCCGTTCTCCTGTTCAAGCCGCGCGGCCTTTTCCCGCGCG
CGGTGGATTGAGGTGCATCG

Downstream 100 bases:

>100_bases
CATTACCACCGACGTACTCATCATCGGCACCGGCCCAGCAGGCTCTGCCACCGCTGCTCTGCTCTCGTCCTACGGGGTCG
AGAACCTAGTGATCAACCGC

Product: putative permease component of ABC transporter

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: LIV-I protein M [H]

Number of amino acids: Translated: 361; Mature: 360

Protein sequence:

>361_residues
MTMAIPSFTVETRTRASTLSAVAVVALLLLAFAAPFIVARGVIQDLFFILTMLVLAQNWNLLAGYAGLISVGQQVFVGFG
AYTMFGCVILFGVDPVVAILIAGVFSVALAIPTAFFTFRLYGPYFAIGTWVVAEVVRLLLAQWKALGGGTGTSLPREATR
DMMGVGAMRDLFGMKASEAGDALTYWLALILAVATIGFIYGLLRSKQGLGLAAVRDNEQAARALGVDARRMKTLVYLATA
FMTGIAGALIYVQKARISPDAAFSVTDWTAYVIFIVVIGGIGTIEGPILGVIIFFLLQNLLADYGSWYLLMLGLLGIAIM
LLAPRGLWGLFSERTGIQLFPVRRVLKGGPLNLTHRGGTHG

Sequences:

>Translated_361_residues
MTMAIPSFTVETRTRASTLSAVAVVALLLLAFAAPFIVARGVIQDLFFILTMLVLAQNWNLLAGYAGLISVGQQVFVGFG
AYTMFGCVILFGVDPVVAILIAGVFSVALAIPTAFFTFRLYGPYFAIGTWVVAEVVRLLLAQWKALGGGTGTSLPREATR
DMMGVGAMRDLFGMKASEAGDALTYWLALILAVATIGFIYGLLRSKQGLGLAAVRDNEQAARALGVDARRMKTLVYLATA
FMTGIAGALIYVQKARISPDAAFSVTDWTAYVIFIVVIGGIGTIEGPILGVIIFFLLQNLLADYGSWYLLMLGLLGIAIM
LLAPRGLWGLFSERTGIQLFPVRRVLKGGPLNLTHRGGTHG
>Mature_360_residues
TMAIPSFTVETRTRASTLSAVAVVALLLLAFAAPFIVARGVIQDLFFILTMLVLAQNWNLLAGYAGLISVGQQVFVGFGA
YTMFGCVILFGVDPVVAILIAGVFSVALAIPTAFFTFRLYGPYFAIGTWVVAEVVRLLLAQWKALGGGTGTSLPREATRD
MMGVGAMRDLFGMKASEAGDALTYWLALILAVATIGFIYGLLRSKQGLGLAAVRDNEQAARALGVDARRMKTLVYLATAF
MTGIAGALIYVQKARISPDAAFSVTDWTAYVIFIVVIGGIGTIEGPILGVIIFFLLQNLLADYGSWYLLMLGLLGIAIML
LAPRGLWGLFSERTGIQLFPVRRVLKGGPLNLTHRGGTHG

Specific function: Part of the binding-protein-dependent transport system for branched-chain amino acids. Probably responsible for the translocation of the substrates across the membrane [H]

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=307, Percent_Identity=30.6188925081433, Blast_Score=103, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 38585; Mature: 38453

Theoretical pI: Translated: 10.04; Mature: 10.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMAIPSFTVETRTRASTLSAVAVVALLLLAFAAPFIVARGVIQDLFFILTMLVLAQNWN
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
LLAGYAGLISVGQQVFVGFGAYTMFGCVILFGVDPVVAILIAGVFSVALAIPTAFFTFRL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YGPYFAIGTWVVAEVVRLLLAQWKALGGGTGTSLPREATRDMMGVGAMRDLFGMKASEAG
HCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCC
DALTYWLALILAVATIGFIYGLLRSKQGLGLAAVRDNEQAARALGVDARRMKTLVYLATA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHCCCHHHHHHHHHHHHH
FMTGIAGALIYVQKARISPDAAFSVTDWTAYVIFIVVIGGIGTIEGPILGVIIFFLLQNL
HHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
LADYGSWYLLMLGLLGIAIMLLAPRGLWGLFSERTGIQLFPVRRVLKGGPLNLTHRGGTH
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCEEHHHHHHHCCCCCEEECCCCCC
G
C
>Mature Secondary Structure 
TMAIPSFTVETRTRASTLSAVAVVALLLLAFAAPFIVARGVIQDLFFILTMLVLAQNWN
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
LLAGYAGLISVGQQVFVGFGAYTMFGCVILFGVDPVVAILIAGVFSVALAIPTAFFTFRL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YGPYFAIGTWVVAEVVRLLLAQWKALGGGTGTSLPREATRDMMGVGAMRDLFGMKASEAG
HCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCC
DALTYWLALILAVATIGFIYGLLRSKQGLGLAAVRDNEQAARALGVDARRMKTLVYLATA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHCCCHHHHHHHHHHHHH
FMTGIAGALIYVQKARISPDAAFSVTDWTAYVIFIVVIGGIGTIEGPILGVIIFFLLQNL
HHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
LADYGSWYLLMLGLLGIAIMLLAPRGLWGLFSERTGIQLFPVRRVLKGGPLNLTHRGGTH
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCEEHHHHHHHCCCCCEEECCCCCC
G
C

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2195019; 8041620; 9278503 [H]