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The map label for this gene is braD [H]

Identifier: 116248772

GI number: 116248772

Start: 92294

End: 93157

Strand: Reverse

Name: braD [H]

Synonym: pRL120096

Alternate gene names: 116248772

Gene position: 93157-92294 (Counterclockwise)

Preceding gene: 116248773

Following gene: 116248771

Centisome position: 10.71

GC content: 63.08

Gene sequence:

>864_bases
ATGAACTGGCTTGATACCATTCTCCAGGGCGTGCTGCTCGGCGGGCTCTATGCGCTGTTTGCGGCGGGCTTGAGCCTCGT
CTTCGGCATCATGCGGCTGGTCAACCTCGCCCATGGCGACCTGATCGTGCTTGCCGCCTTCTTGATTCTGATCCTGGTGA
CAACACTCGGGCTTGACCCGTTCGTTGCCGCCCTGATCGCGATGCCGTTGATGTTCGCCATCGGCTGGGGCCTGCAATAT
TTCCTGCTCAACCGCACTCTCGGCAAGGATATCCTGCCGCCGCTGCTTGTCACCTTCGGCTTGTCGATCGTCATCCAGAA
CGGCCTGCTCGAAGGATTTTCGGCCGATAGCCGCCGTGTTTTTGCCGGCAGTCTCGAGAGCGCCTCCGTCAATCTAGGTC
CTGTCACCGCCGGCATCATGCCGCTGATGACCTTCTTTTCCGCCGTCGCCGTCATCGTCTGTCTCAACCAGCTCATCTAC
CGCACCTCCGTCGGCCGCGCCTTCCGGGCAACCTCGGACGATCCGGTGACGGCAGGGCTGATGGGCATTCGGCCGCAGCG
CATCTTCGCGATGGCAACGGGGCTCGCCATGGTCGTGGTGACGATCGCTGCCCTCTATCTCGGGACACGCGCCAATTTCG
ATCCGACGTCAGGGCCAGCGCGACTGATCTACGCCTTCGAAGCGGTGATCATCGGCGGCCTCGGCTCGCTCTGGGGCACG
CTCGCCGGCGGCATCATCCTCGGCGTTGCCCAGACTGTGGGTGCCGCGATCAATCCGGAATGGCAGATCCTTGCCGGCCA
CCTGGCATTCCTCGCCGTTCTCCTGTTCAAGCCGCGCGGCCTTTTCCCGCGCGCGGTGGATTGA

Upstream 100 bases:

>100_bases
CGCGTCTACTGCATGATGGAGGGGCGAGTGACGCTGTCGGGCCGCACTGCCGATCTTAGCCGCGCCGACATCCACAAGGC
CTATTTCGGGACCGATCATC

Downstream 100 bases:

>100_bases
GGTGCATCGATGACCATGGCCATTCCATCCTTCACCGTCGAGACGCGTACCAGGGCATCGACATTGTCGGCTGTCGCGGT
CGTTGCCCTTCTGCTGCTTG

Product: putative permease component of ABC transporter

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 287; Mature: 287

Protein sequence:

>287_residues
MNWLDTILQGVLLGGLYALFAAGLSLVFGIMRLVNLAHGDLIVLAAFLILILVTTLGLDPFVAALIAMPLMFAIGWGLQY
FLLNRTLGKDILPPLLVTFGLSIVIQNGLLEGFSADSRRVFAGSLESASVNLGPVTAGIMPLMTFFSAVAVIVCLNQLIY
RTSVGRAFRATSDDPVTAGLMGIRPQRIFAMATGLAMVVVTIAALYLGTRANFDPTSGPARLIYAFEAVIIGGLGSLWGT
LAGGIILGVAQTVGAAINPEWQILAGHLAFLAVLLFKPRGLFPRAVD

Sequences:

>Translated_287_residues
MNWLDTILQGVLLGGLYALFAAGLSLVFGIMRLVNLAHGDLIVLAAFLILILVTTLGLDPFVAALIAMPLMFAIGWGLQY
FLLNRTLGKDILPPLLVTFGLSIVIQNGLLEGFSADSRRVFAGSLESASVNLGPVTAGIMPLMTFFSAVAVIVCLNQLIY
RTSVGRAFRATSDDPVTAGLMGIRPQRIFAMATGLAMVVVTIAALYLGTRANFDPTSGPARLIYAFEAVIIGGLGSLWGT
LAGGIILGVAQTVGAAINPEWQILAGHLAFLAVLLFKPRGLFPRAVD
>Mature_287_residues
MNWLDTILQGVLLGGLYALFAAGLSLVFGIMRLVNLAHGDLIVLAAFLILILVTTLGLDPFVAALIAMPLMFAIGWGLQY
FLLNRTLGKDILPPLLVTFGLSIVIQNGLLEGFSADSRRVFAGSLESASVNLGPVTAGIMPLMTFFSAVAVIVCLNQLIY
RTSVGRAFRATSDDPVTAGLMGIRPQRIFAMATGLAMVVVTIAALYLGTRANFDPTSGPARLIYAFEAVIIGGLGSLWGT
LAGGIILGVAQTVGAAINPEWQILAGHLAFLAVLLFKPRGLFPRAVD

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0559

COG function: function code E; Branched-chain amino acid ABC-type transport system, permease components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789866, Length=307, Percent_Identity=28.3387622149837, Blast_Score=95, Evalue=6e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851 [H]

Pfam domain/function: PF02653 BPD_transp_2 [H]

EC number: NA

Molecular weight: Translated: 30272; Mature: 30272

Theoretical pI: Translated: 9.07; Mature: 9.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNWLDTILQGVLLGGLYALFAAGLSLVFGIMRLVNLAHGDLIVLAAFLILILVTTLGLDP
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCH
FVAALIAMPLMFAIGWGLQYFLLNRTLGKDILPPLLVTFGLSIVIQNGLLEGFSADSRRV
HHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEE
FAGSLESASVNLGPVTAGIMPLMTFFSAVAVIVCLNQLIYRTSVGRAFRATSDDPVTAGL
EECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCCCCCHHHH
MGIRPQRIFAMATGLAMVVVTIAALYLGTRANFDPTSGPARLIYAFEAVIIGGLGSLWGT
HCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LAGGIILGVAQTVGAAINPEWQILAGHLAFLAVLLFKPRGLFPRAVD
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MNWLDTILQGVLLGGLYALFAAGLSLVFGIMRLVNLAHGDLIVLAAFLILILVTTLGLDP
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCH
FVAALIAMPLMFAIGWGLQYFLLNRTLGKDILPPLLVTFGLSIVIQNGLLEGFSADSRRV
HHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEE
FAGSLESASVNLGPVTAGIMPLMTFFSAVAVIVCLNQLIYRTSVGRAFRATSDDPVTAGL
EECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCCCCCHHHH
MGIRPQRIFAMATGLAMVVVTIAALYLGTRANFDPTSGPARLIYAFEAVIIGGLGSLWGT
HCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LAGGIILGVAQTVGAAINPEWQILAGHLAFLAVLLFKPRGLFPRAVD
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043; 2509433 [H]