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The map label for this gene is tfdB [H]

Identifier: 116248770

GI number: 116248770

Start: 89452

End: 91206

Strand: Reverse

Name: tfdB [H]

Synonym: pRL120094

Alternate gene names: 116248770

Gene position: 91206-89452 (Counterclockwise)

Preceding gene: 116248771

Following gene: 116248769

Centisome position: 10.48

GC content: 62.79

Gene sequence:

>1755_bases
ATGGCTGACATTACCACCGACGTACTCATCATCGGCACCGGCCCAGCAGGCTCTGCCACCGCTGCTCTGCTCTCGTCCTA
CGGGGTCGAGAACCTAGTGATCAACCGCTACCGCTGGCTCGCCAGCACGCCGCGCGCCCACATCACCAACCAGCGTACCA
TGGAAGTGCTGCGCGATCTCGGCCGCGACGTGGAGGACGAGGCCTATATGTTTGCGGCCGAGCAGGACCTGATGGGTGAG
AACGTGTTTTGCACCTCGCTCACCGGCGAAGAGATCGGCCGGATGAAGAGCTGGGGCAAACATCCGCTGTCGCGCGCCGA
GCACCAGTTGTCGTCGCCGACCCATATGAACGACCTGCCGCAGACCTTCATGGAGCCGCTGCTCTTCAAGACGGCTTGTT
CGCGCGGCAGCCAATCGCGCATGTCGACGGAATATCTGAGCCATGCGCAGGACGAAGACGGGGTCACGACGACCTGTCTC
GACCGGCTGACCGGCAAGGAATTTACCGTCCGCTCGAAATATCTGGTCGGCGCCGATGGCGGCAATTCCAAGGTTGCGGA
ACATGCGGGCCTCACCTTCGAAGGCAAGATGGGCGTTGCCGGCTCGATGAACATTCTGTTCAAAGCCGATCTGTCGCGGC
TGGTCGCCCATCGTCCATCGGTGCTTTACTGGGTGCTGCAGCCGGGTGCCGATGTCGGCGGCATCGGCATGGGGCTGGTG
CGCATGGTCAGGCCTTGGAACGAATGGCTGATCGTGTGGGGTTACGACATCAACCAGCCGGCGCCTGAGGTGGACGAAGC
GCATGCGAAGAAGGTCGTGCGCGACCTGGTCGGCGATCCCGATCTCGAGATGACCATCAAGTCGGTCTCGACCTGGACCG
TCAACAATATGTACGCTACGTCAATGTCGAATGGCCGCGTCTTCTGCATGGGCGACGCCACCCACCGCCATCCGCCGTCG
AACGGCCTCGGCTCCAACACCTCGATTCAGGACGCTTTCAATCTCGCCTGGAAGCTCGCCTTCGTCCTGAAGGGGGCCGC
AGGCCCGAAACTTCTCGACAGCTATCAGGCCGAGCGCGCGCCCGTTGCCAAGCAGATCGTTAGACGCGCCAACAAGTCGA
TCGAGGAGTTCGGGCCGATCTTCAAGGCGCTGGGGCTGCTCGATTCCATCGACCCGGTGAAGATGCAGCAGAACATGGAC
GCCCGCTGCAACAACACGGCTGCTGCCGAGGAGCAACGGGCCGCAATCCGCAAGGCGATCGCCGATAAGGTCTACGAGTT
CGATTGCCACGGGGTCGAGATGAACCAGCGCTACCACTCCAGTGCTGCGATATCAGATGGACAGGCCGAGCCCGCCTTTA
CCAAGGACCCGGAACTGCATTGCCAACAGACGAGCTGGCCCGGCGCGCGGCTGCCGCATGCCTGGCTCTTCTCTGCGACC
GGCGAGAAGGTTTCGACGCTCGACCTCACCGGCCACGGCAAATTGACCGTGCTGACCGGCATCGGCGGGCAGGGCTGGGT
CGAGGCGGCCCGCAGTCTCGGCAACAAATTCGGCATTGAGATCGTTGCCCAGGTCATCGGGCCCCGCCAGCCGTGGCAGG
ATTTTACCGGCGACTGGGCCAATATCCGCGAAATACGCGACAGCGGCATCCTGCTGGTTCGTCCCGACCACCACGTCGCC
TGGCGGCGCGAGGCGATGGTCGACGATCCGACGACTGAACTGCGCCGCGCGCTGACAGCAATTCTTGGAAAGTGA

Upstream 100 bases:

>100_bases
CTCTGGGGATTGTTCTCCGAGCGCACCGGCATCCAGCTTTTCCCGGTTCGCCGCGTCCTGAAGGGCGGGCCGCTCAATCT
CACGCATAGGGGAGGGACTC

Downstream 100 bases:

>100_bases
GGCCGTGATGGACGCGCATGAGAAAGGCTTCTTCACCGAAGAGATCTCCGTCGAGGTCGTCACCGGCCGCAACAAGAATG
CCAAGGACGAGCGGCTGAAG

Product: putative dichlorophenol monooxygenase/hydroxylase

Products: NA

Alternate protein names: 2,4-dichlorophenol hydroxylase; 2,4-DCP hydroxylase [H]

Number of amino acids: Translated: 584; Mature: 583

Protein sequence:

>584_residues
MADITTDVLIIGTGPAGSATAALLSSYGVENLVINRYRWLASTPRAHITNQRTMEVLRDLGRDVEDEAYMFAAEQDLMGE
NVFCTSLTGEEIGRMKSWGKHPLSRAEHQLSSPTHMNDLPQTFMEPLLFKTACSRGSQSRMSTEYLSHAQDEDGVTTTCL
DRLTGKEFTVRSKYLVGADGGNSKVAEHAGLTFEGKMGVAGSMNILFKADLSRLVAHRPSVLYWVLQPGADVGGIGMGLV
RMVRPWNEWLIVWGYDINQPAPEVDEAHAKKVVRDLVGDPDLEMTIKSVSTWTVNNMYATSMSNGRVFCMGDATHRHPPS
NGLGSNTSIQDAFNLAWKLAFVLKGAAGPKLLDSYQAERAPVAKQIVRRANKSIEEFGPIFKALGLLDSIDPVKMQQNMD
ARCNNTAAAEEQRAAIRKAIADKVYEFDCHGVEMNQRYHSSAAISDGQAEPAFTKDPELHCQQTSWPGARLPHAWLFSAT
GEKVSTLDLTGHGKLTVLTGIGGQGWVEAARSLGNKFGIEIVAQVIGPRQPWQDFTGDWANIREIRDSGILLVRPDHHVA
WRREAMVDDPTTELRRALTAILGK

Sequences:

>Translated_584_residues
MADITTDVLIIGTGPAGSATAALLSSYGVENLVINRYRWLASTPRAHITNQRTMEVLRDLGRDVEDEAYMFAAEQDLMGE
NVFCTSLTGEEIGRMKSWGKHPLSRAEHQLSSPTHMNDLPQTFMEPLLFKTACSRGSQSRMSTEYLSHAQDEDGVTTTCL
DRLTGKEFTVRSKYLVGADGGNSKVAEHAGLTFEGKMGVAGSMNILFKADLSRLVAHRPSVLYWVLQPGADVGGIGMGLV
RMVRPWNEWLIVWGYDINQPAPEVDEAHAKKVVRDLVGDPDLEMTIKSVSTWTVNNMYATSMSNGRVFCMGDATHRHPPS
NGLGSNTSIQDAFNLAWKLAFVLKGAAGPKLLDSYQAERAPVAKQIVRRANKSIEEFGPIFKALGLLDSIDPVKMQQNMD
ARCNNTAAAEEQRAAIRKAIADKVYEFDCHGVEMNQRYHSSAAISDGQAEPAFTKDPELHCQQTSWPGARLPHAWLFSAT
GEKVSTLDLTGHGKLTVLTGIGGQGWVEAARSLGNKFGIEIVAQVIGPRQPWQDFTGDWANIREIRDSGILLVRPDHHVA
WRREAMVDDPTTELRRALTAILGK
>Mature_583_residues
ADITTDVLIIGTGPAGSATAALLSSYGVENLVINRYRWLASTPRAHITNQRTMEVLRDLGRDVEDEAYMFAAEQDLMGEN
VFCTSLTGEEIGRMKSWGKHPLSRAEHQLSSPTHMNDLPQTFMEPLLFKTACSRGSQSRMSTEYLSHAQDEDGVTTTCLD
RLTGKEFTVRSKYLVGADGGNSKVAEHAGLTFEGKMGVAGSMNILFKADLSRLVAHRPSVLYWVLQPGADVGGIGMGLVR
MVRPWNEWLIVWGYDINQPAPEVDEAHAKKVVRDLVGDPDLEMTIKSVSTWTVNNMYATSMSNGRVFCMGDATHRHPPSN
GLGSNTSIQDAFNLAWKLAFVLKGAAGPKLLDSYQAERAPVAKQIVRRANKSIEEFGPIFKALGLLDSIDPVKMQQNMDA
RCNNTAAAEEQRAAIRKAIADKVYEFDCHGVEMNQRYHSSAAISDGQAEPAFTKDPELHCQQTSWPGARLPHAWLFSATG
EKVSTLDLTGHGKLTVLTGIGGQGWVEAARSLGNKFGIEIVAQVIGPRQPWQDFTGDWANIREIRDSGILLVRPDHHVAW
RREAMVDDPTTELRRALTAILGK

Specific function: Transforms 2,4-dichlorophenol (2,4-DCP) into 3,5- dichlorocatechol [H]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pheA/tfdB FAD monooxygenase family [H]

Homologues:

Organism=Escherichia coli, GI1786543, Length=370, Percent_Identity=26.7567567567568, Blast_Score=87, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: =1.14.13.20 [H]

Molecular weight: Translated: 64217; Mature: 64085

Theoretical pI: Translated: 6.56; Mature: 6.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADITTDVLIIGTGPAGSATAALLSSYGVENLVINRYRWLASTPRAHITNQRTMEVLRDL
CCCCCCCEEEEECCCCCHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHCCCHHHHHHHHHH
GRDVEDEAYMFAAEQDLMGENVFCTSLTGEEIGRMKSWGKHPLSRAEHQLSSPTHMNDLP
CCCCCCCCEEEEEHHHHCCCCEEEEECCHHHHHHHHHCCCCHHHHHHHHCCCCCCCHHHH
QTFMEPLLFKTACSRGSQSRMSTEYLSHAQDEDGVTTTCLDRLTGKEFTVRSKYLVGADG
HHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCEEEECEEEEECCC
GNSKVAEHAGLTFEGKMGVAGSMNILFKADLSRLVAHRPSVLYWVLQPGADVGGIGMGLV
CCCCHHHHCCCEEECCCCCCCCCCEEEHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHH
RMVRPWNEWLIVWGYDINQPAPEVDEAHAKKVVRDLVGDPDLEMTIKSVSTWTVNNMYAT
HHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEHHEEEEECCEEEE
SMSNGRVFCMGDATHRHPPSNGLGSNTSIQDAFNLAWKLAFVLKGAAGPKLLDSYQAERA
ECCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCC
PVAKQIVRRANKSIEEFGPIFKALGLLDSIDPVKMQQNMDARCNNTAAAEEQRAAIRKAI
HHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHH
ADKVYEFDCHGVEMNQRYHSSAAISDGQAEPAFTKDPELHCQQTSWPGARLPHAWLFSAT
HHHHHCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCEEEECC
GEKVSTLDLTGHGKLTVLTGIGGQGWVEAARSLGNKFGIEIVAQVIGPRQPWQDFTGDWA
CCEEEEEEECCCCCEEEEEECCCHHHHHHHHHHCHHHHHHHHHHHHCCCCCCHHHCCCHH
NIREIRDSGILLVRPDHHVAWRREAMVDDPTTELRRALTAILGK
HHHHHCCCCEEEECCCCCHHHHHHHCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
ADITTDVLIIGTGPAGSATAALLSSYGVENLVINRYRWLASTPRAHITNQRTMEVLRDL
CCCCCCEEEEECCCCCHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHCCCHHHHHHHHHH
GRDVEDEAYMFAAEQDLMGENVFCTSLTGEEIGRMKSWGKHPLSRAEHQLSSPTHMNDLP
CCCCCCCCEEEEEHHHHCCCCEEEEECCHHHHHHHHHCCCCHHHHHHHHCCCCCCCHHHH
QTFMEPLLFKTACSRGSQSRMSTEYLSHAQDEDGVTTTCLDRLTGKEFTVRSKYLVGADG
HHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCEEEECEEEEECCC
GNSKVAEHAGLTFEGKMGVAGSMNILFKADLSRLVAHRPSVLYWVLQPGADVGGIGMGLV
CCCCHHHHCCCEEECCCCCCCCCCEEEHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHH
RMVRPWNEWLIVWGYDINQPAPEVDEAHAKKVVRDLVGDPDLEMTIKSVSTWTVNNMYAT
HHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEHHEEEEECCEEEE
SMSNGRVFCMGDATHRHPPSNGLGSNTSIQDAFNLAWKLAFVLKGAAGPKLLDSYQAERA
ECCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCC
PVAKQIVRRANKSIEEFGPIFKALGLLDSIDPVKMQQNMDARCNNTAAAEEQRAAIRKAI
HHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHH
ADKVYEFDCHGVEMNQRYHSSAAISDGQAEPAFTKDPELHCQQTSWPGARLPHAWLFSAT
HHHHHCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCEEEECC
GEKVSTLDLTGHGKLTVLTGIGGQGWVEAARSLGNKFGIEIVAQVIGPRQPWQDFTGDWA
CCEEEEEEECCCCCEEEEEECCCHHHHHHHHHHCHHHHHHHHHHHHCCCCCCHHHCCCHH
NIREIRDSGILLVRPDHHVAWRREAMVDDPTTELRRALTAILGK
HHHHHCCCCEEEECCCCCHHHHHHHCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12949179; 11572451 [H]