Click here to switch to the map view.

The map label for this gene is thuE [C]

Identifier: 116248711

GI number: 116248711

Start: 34035

End: 35297

Strand: Direct

Name: thuE [C]

Synonym: pRL120035

Alternate gene names: 116248711

Gene position: 34035-35297 (Clockwise)

Preceding gene: 116248710

Following gene: 116248712

Centisome position: 3.91

GC content: 57.48

Gene sequence:

>1263_bases
ATGAAAAAGTTTGCTTTAGCTTTGTCGGCAATCAGCGTCGCTATCGCTGCCGGCTCTGTCCGTGCCGCTGAAATTTCATT
TGCCGCAAATACCACCGGCAAAAACGTCGAATTCCTACACAACCAGCTCGCAATTTTCGAGAAGAATACCGGCAATCATG
TCAAGCTCGTCACCATGCCGTCATCGAGCAGCGAGCAGTTTTCCCAATATCGCCTGTGGCTTGCTGCTGGAAACAAGGAC
GTCGATGTCTATCAGACCGATGTTATCTGGGCGCCGCAGCTTGCCGACCAGTTCGTCGATCTGAAGGAGGCAGCCAAGGA
CGTGGTCGGCCAGTTCTTCCCGTCGATCATCGCGTCTCAGACTGTCAATGGCCGGCTTGTGGCGCTGCCGTTGTTTACCG
ATGCTCCAGCGCTGTTTTACCGAAAGGACCTGCTGGAGAAATATGGAAAACAGCCGCCGAAGACCTGGGACGAGATGGCC
GCAACCGCCAAAGAGATTCAGGATAAAGAACGCCAGGCCGGACAGAAGGACCTCTGGGGCTACGTCTTCCAGGGCAATTC
CTACGAAGGGCTTACCTGCAACGGACTGGAATGGGTGAAGTCGTCGGGCGGCGGCCAGATCGTCGAGCCGGACGGGACGA
TCTCGATCAATAACGAGAAGGCGGCAGCAGCGCTTGAACGGGCCAAGGACTGGATCGGCACGATCTCACCGCCAGGGGTG
CTCGCCTATCAGGAAGAAGAATCGCGCGGCGTCTGGCAGACCGGCAACGCCGTCTTCATGCGCAACTGGCCTTACGCCTA
TTCTCTCGGAAACGGTGCCGACAGCGCGGTGAAGGGCAAGTTCGACGTGATGACGCTACCGGTTGCGGCTGTCGGCGATA
AGCCGTCTTCGACACTTGGCGGCTGGAACCTGGCGGTGTCGAAATATTCCGAACAGCAGGACGCAGCCATCGCGCTCGTA
AAATTCCTGGCGTCGAAGGACGTTCAAAAGGCGCGTGCCATCGAACTCTCCAATTTGCCGACGCTGACGGATCTTTATGA
CGACAAGGATGTCGCTGCCGCGCAGCCCTTCATGCCGAACTGGAAACCCATTTTCCAGGACGCGGTGCCACGCCCTTCGG
CGACGGCCAAGGTAAAGTACAACGAGGTGTCCTCAAAGTTCTGGACTGCAGTGCACAATACCTTGTCAGGCAGCGGCTCG
GCTGCCGAGAACCTCGAGCTTCTCGAAGCCGACCTAACGACCCTCAAAGGCGACGCTTGGTGA

Upstream 100 bases:

>100_bases
CATCCACCGCACGTCCAGCGATAAGCTGTAGCGTCAGCGCCTCCGCGACCTTCTGCAATGGTCCGATTGCCAAATCGATT
TCAATTTCTGGGAGGAGATT

Downstream 100 bases:

>100_bases
GTATTCCGACCGGCGGCTGTCAGGCCGCCGGTCCGGTCTTCGGACGCGCTAACGAGGGATAGACGTTTCATGAGCGAAAT
CGTAGTTCCACAAGCAGTCA

Product: putative substrate-binding component of ABC transporter

Products: ADP; phosphate; maltose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 420; Mature: 420

Protein sequence:

>420_residues
MKKFALALSAISVAIAAGSVRAAEISFAANTTGKNVEFLHNQLAIFEKNTGNHVKLVTMPSSSSEQFSQYRLWLAAGNKD
VDVYQTDVIWAPQLADQFVDLKEAAKDVVGQFFPSIIASQTVNGRLVALPLFTDAPALFYRKDLLEKYGKQPPKTWDEMA
ATAKEIQDKERQAGQKDLWGYVFQGNSYEGLTCNGLEWVKSSGGGQIVEPDGTISINNEKAAAALERAKDWIGTISPPGV
LAYQEEESRGVWQTGNAVFMRNWPYAYSLGNGADSAVKGKFDVMTLPVAAVGDKPSSTLGGWNLAVSKYSEQQDAAIALV
KFLASKDVQKARAIELSNLPTLTDLYDDKDVAAAQPFMPNWKPIFQDAVPRPSATAKVKYNEVSSKFWTAVHNTLSGSGS
AAENLELLEADLTTLKGDAW

Sequences:

>Translated_420_residues
MKKFALALSAISVAIAAGSVRAAEISFAANTTGKNVEFLHNQLAIFEKNTGNHVKLVTMPSSSSEQFSQYRLWLAAGNKD
VDVYQTDVIWAPQLADQFVDLKEAAKDVVGQFFPSIIASQTVNGRLVALPLFTDAPALFYRKDLLEKYGKQPPKTWDEMA
ATAKEIQDKERQAGQKDLWGYVFQGNSYEGLTCNGLEWVKSSGGGQIVEPDGTISINNEKAAAALERAKDWIGTISPPGV
LAYQEEESRGVWQTGNAVFMRNWPYAYSLGNGADSAVKGKFDVMTLPVAAVGDKPSSTLGGWNLAVSKYSEQQDAAIALV
KFLASKDVQKARAIELSNLPTLTDLYDDKDVAAAQPFMPNWKPIFQDAVPRPSATAKVKYNEVSSKFWTAVHNTLSGSGS
AAENLELLEADLTTLKGDAW
>Mature_420_residues
MKKFALALSAISVAIAAGSVRAAEISFAANTTGKNVEFLHNQLAIFEKNTGNHVKLVTMPSSSSEQFSQYRLWLAAGNKD
VDVYQTDVIWAPQLADQFVDLKEAAKDVVGQFFPSIIASQTVNGRLVALPLFTDAPALFYRKDLLEKYGKQPPKTWDEMA
ATAKEIQDKERQAGQKDLWGYVFQGNSYEGLTCNGLEWVKSSGGGQIVEPDGTISINNEKAAAALERAKDWIGTISPPGV
LAYQEEESRGVWQTGNAVFMRNWPYAYSLGNGADSAVKGKFDVMTLPVAAVGDKPSSTLGGWNLAVSKYSEQQDAAIALV
KFLASKDVQKARAIELSNLPTLTDLYDDKDVAAAQPFMPNWKPIFQDAVPRPSATAKVKYNEVSSKFWTAVHNTLSGSGS
AAENLELLEADLTTLKGDAW

Specific function: Probably part of a binding-protein-dependent transport system [H]

COG id: COG1653

COG function: function code G; ABC-type sugar transport system, periplasmic component

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 1 family [H]

Homologues:

None

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 720 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 240 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 45660; Mature: 45660

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKFALALSAISVAIAAGSVRAAEISFAANTTGKNVEFLHNQLAIFEKNTGNHVKLVTMP
CCHHHHHHHHHHHHHHCCCEEEEEEEEEECCCCCCHHHHHHEEEEEECCCCCEEEEEECC
SSSSEQFSQYRLWLAAGNKDVDVYQTDVIWAPQLADQFVDLKEAAKDVVGQFFPSIIASQ
CCCHHHHHHEEEEEEECCCCCEEEEECEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
TVNGRLVALPLFTDAPALFYRKDLLEKYGKQPPKTWDEMAATAKEIQDKERQAGQKDLWG
CCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCE
YVFQGNSYEGLTCNGLEWVKSSGGGQIVEPDGTISINNEKAAAALERAKDWIGTISPPGV
EEEECCCCCCEEECCHHHHHCCCCCEEECCCCEEEECCHHHHHHHHHHHHHHCCCCCCCE
LAYQEEESRGVWQTGNAVFMRNWPYAYSLGNGADSAVKGKFDVMTLPVAAVGDKPSSTLG
EEEECCCCCCCEECCCEEEEECCCEEEECCCCCCCCCCCCEEEEEECEEECCCCCCCCCC
GWNLAVSKYSEQQDAAIALVKFLASKDVQKARAIELSNLPTLTDLYDDKDVAAAQPFMPN
CEEEEEHHCCCHHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHCCCCCCCCCCCCCCC
WKPIFQDAVPRPSATAKVKYNEVSSKFWTAVHNTLSGSGSAAENLELLEADLTTLKGDAW
CCHHHHHCCCCCCCEEEEEEHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKKFALALSAISVAIAAGSVRAAEISFAANTTGKNVEFLHNQLAIFEKNTGNHVKLVTMP
CCHHHHHHHHHHHHHHCCCEEEEEEEEEECCCCCCHHHHHHEEEEEECCCCCEEEEEECC
SSSSEQFSQYRLWLAAGNKDVDVYQTDVIWAPQLADQFVDLKEAAKDVVGQFFPSIIASQ
CCCHHHHHHEEEEEEECCCCCEEEEECEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
TVNGRLVALPLFTDAPALFYRKDLLEKYGKQPPKTWDEMAATAKEIQDKERQAGQKDLWG
CCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCE
YVFQGNSYEGLTCNGLEWVKSSGGGQIVEPDGTISINNEKAAAALERAKDWIGTISPPGV
EEEECCCCCCEEECCHHHHHCCCCCEEECCCCEEEECCHHHHHHHHHHHHHHCCCCCCCE
LAYQEEESRGVWQTGNAVFMRNWPYAYSLGNGADSAVKGKFDVMTLPVAAVGDKPSSTLG
EEEECCCCCCCEECCCEEEEECCCEEEECCCCCCCCCCCCEEEEEECEEECCCCCCCCCC
GWNLAVSKYSEQQDAAIALVKFLASKDVQKARAIELSNLPTLTDLYDDKDVAAAQPFMPN
CEEEEEHHCCCHHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHCCCCCCCCCCCCCCC
WKPIFQDAVPRPSATAKVKYNEVSSKFWTAVHNTLSGSGSAAENLELLEADLTTLKGDAW
CCHHHHHCCCCCCCEEEEEEHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; maltose [Periplasm]; H2O [C]

Specific reaction: ATP + maltose [Periplasm] + H2O = ADP + phosphate + maltose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10567266 [H]