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The map label for this gene is glcK [H]

Identifier: 116248710

GI number: 116248710

Start: 32716

End: 33681

Strand: Direct

Name: glcK [H]

Synonym: pRL120034

Alternate gene names: 116248710

Gene position: 32716-33681 (Clockwise)

Preceding gene: 116248709

Following gene: 116248711

Centisome position: 3.76

GC content: 63.15

Gene sequence:

>966_bases
ATGCAGCAGGTTGCCATTGGAATCGACCTCGGCGGGACGCAGGTGCGCGCTGCCCTCGTCGACGAGCAGGGCAGGATCCT
TGCCCGCGCAGCCGAACCGACGGATGCGCTGGCAGGCCCCGACCGGGTGCTCGCCCAAATCTGCGGCCTGACCGATGGAC
TGCTTGCAGCATCAAATCCTGCTTCGGTGGTGGGCGTCGGCGTATCCGCTCCAGGCCCGCTCGATACGGTCGCCGGTGTC
GCCTCGAATATCCCGACCCTCTCCGGCTTTGTCGACTTTCCGCTGAAGGCGGAGTTGCAGAAGCGGTTTCCGTTTCCGGT
CGACCTCGAGAATGATGCAATAGCCGCTGCCATCGGCGAGTGGCAGTTCGGAGCCGGCAAGGGCCTGGATAATCTGGTCT
ATGTCACCGTGAGCACCGGCATTGGCGGTGGCGTTGTGTCGGATGGTCGCGTCGTGCGCGGCCGCAAGGGCATGGCAGCC
CATGTCGGGCACATGTCGGTCGTGCCGAACGGAGAGCTTTGTCCCTGCGGCAACCGGGGTTGTTTCGAGGCCTACGGATC
CGGAACGGCTTTTGCGCGCCGCGCCCAAATGAGGGCGATGGAAACCAGCGGGACGACGATCGGCAGCGACGGCGGCGCCA
TTGACAGCCGCGGCGTTTTCGCGGCCGCAAGGGATGGCGATCGTCTTGCAAATCAACTGGTTGACGAGGAAGCGGAAATT
CTCGGTCGCGGCTTCACCAGCCTGATCCATATCTTCAGTCCGGATATCATCGTGATGGGAGGCGGGCTTTCCCACGAGTT
CGATCGTCTACAACCCGGCATTCAAGCCTACATCACGCAATGGGCAATGCCGGCATTCAGGGATGTCAAAGTGGTGCTGG
CGGCGTTGGATCAGAACTCGGGCCTCGTCGGCGCCGCGGCTCTGGCGTTTCTGACCGGCAAGGTCCCTGCGATCGATCAG
CTTTAG

Upstream 100 bases:

>100_bases
CGGGCGCGGAGACGGTGGTCCTCGGTTCCCTGGCTTTCAATGCGCCGTCGCTCGACGAGCGGATGGCATGGGTGCGCGCA
CTCTAACTCATGGTCTTCAC

Downstream 100 bases:

>100_bases
AAAAGCAGGCCGATCCTGCGTGCGTCGGTAAACGCGCTAGAGCATGATGCCGAAAAGTGTGCGCGGTTTTCGGACGACAT
CCTGATCTATTTCTTTGATT

Product: putative glucokinase

Products: NA

Alternate protein names: Glucose kinase [H]

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MQQVAIGIDLGGTQVRAALVDEQGRILARAAEPTDALAGPDRVLAQICGLTDGLLAASNPASVVGVGVSAPGPLDTVAGV
ASNIPTLSGFVDFPLKAELQKRFPFPVDLENDAIAAAIGEWQFGAGKGLDNLVYVTVSTGIGGGVVSDGRVVRGRKGMAA
HVGHMSVVPNGELCPCGNRGCFEAYGSGTAFARRAQMRAMETSGTTIGSDGGAIDSRGVFAAARDGDRLANQLVDEEAEI
LGRGFTSLIHIFSPDIIVMGGGLSHEFDRLQPGIQAYITQWAMPAFRDVKVVLAALDQNSGLVGAAALAFLTGKVPAIDQ
L

Sequences:

>Translated_321_residues
MQQVAIGIDLGGTQVRAALVDEQGRILARAAEPTDALAGPDRVLAQICGLTDGLLAASNPASVVGVGVSAPGPLDTVAGV
ASNIPTLSGFVDFPLKAELQKRFPFPVDLENDAIAAAIGEWQFGAGKGLDNLVYVTVSTGIGGGVVSDGRVVRGRKGMAA
HVGHMSVVPNGELCPCGNRGCFEAYGSGTAFARRAQMRAMETSGTTIGSDGGAIDSRGVFAAARDGDRLANQLVDEEAEI
LGRGFTSLIHIFSPDIIVMGGGLSHEFDRLQPGIQAYITQWAMPAFRDVKVVLAALDQNSGLVGAAALAFLTGKVPAIDQ
L
>Mature_321_residues
MQQVAIGIDLGGTQVRAALVDEQGRILARAAEPTDALAGPDRVLAQICGLTDGLLAASNPASVVGVGVSAPGPLDTVAGV
ASNIPTLSGFVDFPLKAELQKRFPFPVDLENDAIAAAIGEWQFGAGKGLDNLVYVTVSTGIGGGVVSDGRVVRGRKGMAA
HVGHMSVVPNGELCPCGNRGCFEAYGSGTAFARRAQMRAMETSGTTIGSDGGAIDSRGVFAAARDGDRLANQLVDEEAEI
LGRGFTSLIHIFSPDIIVMGGGLSHEFDRLQPGIQAYITQWAMPAFRDVKVVLAALDQNSGLVGAAALAFLTGKVPAIDQ
L

Specific function: Acts As A Repressor Of The Nagebacd Operon And Acts Both As An Activator And A Repressor For The Transcription Of The Glmsu Operon. [C]

COG id: COG1940

COG function: function code KG; Transcriptional regulator/sugar kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ROK (nagC/xylR) family [H]

Homologues:

Organism=Homo sapiens, GI298566325, Length=270, Percent_Identity=29.6296296296296, Blast_Score=108, Evalue=8e-24,
Organism=Homo sapiens, GI298566315, Length=270, Percent_Identity=29.6296296296296, Blast_Score=108, Evalue=8e-24,
Organism=Homo sapiens, GI4885285, Length=270, Percent_Identity=29.6296296296296, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI190014632, Length=270, Percent_Identity=29.6296296296296, Blast_Score=107, Evalue=1e-23,
Organism=Escherichia coli, GI1786891, Length=313, Percent_Identity=28.7539936102236, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI1787878, Length=246, Percent_Identity=28.8617886178862, Blast_Score=121, Evalue=6e-29,
Organism=Escherichia coli, GI87082230, Length=277, Percent_Identity=33.5740072202166, Blast_Score=100, Evalue=9e-23,
Organism=Escherichia coli, GI87081733, Length=312, Percent_Identity=29.8076923076923, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1787363, Length=320, Percent_Identity=26.875, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI87082127, Length=203, Percent_Identity=23.6453201970443, Blast_Score=79, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000600
- InterPro:   IPR004654 [H]

Pfam domain/function: PF00480 ROK [H]

EC number: =2.7.1.2 [H]

Molecular weight: Translated: 33009; Mature: 33009

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS01125 ROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQQVAIGIDLGGTQVRAALVDEQGRILARAAEPTDALAGPDRVLAQICGLTDGLLAASNP
CCEEEEEEECCCCEEEEEEECCCCCEEEEECCCCHHHCCHHHHHHHHHCCCCCCEECCCC
ASVVGVGVSAPGPLDTVAGVASNIPTLSGFVDFPLKAELQKRFPFPVDLENDAIAAAIGE
CEEEEECCCCCCCHHHHHHHHHCCCCCCCEECCCCHHHHHHCCCCCCCCCCCCEEEEECC
WQFGAGKGLDNLVYVTVSTGIGGGVVSDGRVVRGRKGMAAHVGHMSVVPNGELCPCGNRG
CCCCCCCCCCCEEEEEEECCCCCCCCCCCEEEECCCCCEEECCEEEECCCCCCCCCCCCC
CFEAYGSGTAFARRAQMRAMETSGTTIGSDGGAIDSRGVFAAARDGDRLANQLVDEEAEI
CHHHCCCCHHHHHHHHHHHHHCCCCEECCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHH
LGRGFTSLIHIFSPDIIVMGGGLSHEFDRLQPGIQAYITQWAMPAFRDVKVVLAALDQNS
HHCCHHHHHHHHCCCEEEECCCCCCHHHHHCHHHHHHHHHHCCCHHHHHHHHHHHHCCCC
GLVGAAALAFLTGKVPAIDQL
CCHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MQQVAIGIDLGGTQVRAALVDEQGRILARAAEPTDALAGPDRVLAQICGLTDGLLAASNP
CCEEEEEEECCCCEEEEEEECCCCCEEEEECCCCHHHCCHHHHHHHHHCCCCCCEECCCC
ASVVGVGVSAPGPLDTVAGVASNIPTLSGFVDFPLKAELQKRFPFPVDLENDAIAAAIGE
CEEEEECCCCCCCHHHHHHHHHCCCCCCCEECCCCHHHHHHCCCCCCCCCCCCEEEEECC
WQFGAGKGLDNLVYVTVSTGIGGGVVSDGRVVRGRKGMAAHVGHMSVVPNGELCPCGNRG
CCCCCCCCCCCEEEEEEECCCCCCCCCCCEEEECCCCCEEECCEEEECCCCCCCCCCCCC
CFEAYGSGTAFARRAQMRAMETSGTTIGSDGGAIDSRGVFAAARDGDRLANQLVDEEAEI
CHHHCCCCHHHHHHHHHHHHHCCCCEECCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHH
LGRGFTSLIHIFSPDIIVMGGGLSHEFDRLQPGIQAYITQWAMPAFRDVKVVLAALDQNS
HHCCHHHHHHHHCCCEEEECCCCCCHHHHHCHHHHHHHHHHCCCHHHHHHHHHHHHCCCC
GLVGAAALAFLTGKVPAIDQL
CCHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]