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The map label for this gene is nuoN

Identifier: 114800320

GI number: 114800320

Start: 1799869

End: 1801302

Strand: Direct

Name: nuoN

Synonym: HNE_1758

Alternate gene names: 114800320

Gene position: 1799869-1801302 (Clockwise)

Preceding gene: 114797733

Following gene: 114798329

Centisome position: 48.58

GC content: 55.16

Gene sequence:

>1434_bases
ATGCTTGATACTCAATCCATCATACAGGCCATGGCTCCAGAGCTTTGGCTCGCCGCATCCGGTCTCATGCTGGTGCTCGT
GGGGGCAATCGTTGGAGAGCGCTTCAACAGCCTTTCCTATAAAGTCGGCGCACTCGTCATGTTTGGAGCAGCCGCACTTT
CGGCCATGCATCTTCAGGGCGGCGAAGCCTTCAATGGCCTCGTTAAAACAACAACGTTCACGAACTTTACGAAGGTAATC
AGTTTCACCCTGGCAGGTGTGGCAATTTTCCTCTCCGAAGGGTTCCTGCGCCGGCATGAAATAAGCCGATATGAATACCC
TCTGCTGATGGTCCTTGCTTCGCTTGGTATGGGCATCATTCTGTCGGCAAATAATTTGATGACGCTTTATATGGGCATCG
AAACGCTGAGCCTGTCGTCATATGTGCTGGCAGCGTTCCACCGAGATTCTGCACGGTCGTCTGAAGCGGGCCTCAAATAT
TTCGTGTTGGGCGCACTTGCCTCCGGCATCCTTCTGTATGGTGCCTCACTGGTTTACGGGTTCACCGGCACAACCTCATT
TGATGGCCTGACTTTGGCGGGTGAATCCGTGGGCGCCATGTTTGGCATGGTGCTGATGATCGTCGGTCTCGCGTTCAAGA
TTTCCGCGGCCCCGATGCACGTCTGGACACCTGACGTGTATGAAGGTGCTCCGACGCCTGTTGTCGCTTTTTTTGCTTCA
GCGCCAAAGTTTGCGTCCCTTGTTGTCTTCGCCAACGTGATGTTCACGCTTTTTGGAAGCGTCATGGACCAATGGCAGCT
TATCGTGGCGATCATTGCCGGACTGTCGATGATCGTTGGCTCGCTCGGTGGCCTGACGCAGAATAATATCAAGCGTCTTC
TGGCGTATTCCTCGATCGCCAATGTTGGCTATGCGTTGGTTGCGGTTGCTGCGGGGCAGGTTCTCGGCGCGCCGGCACTT
CTGACGTTCTCGACCATTTATGTCATCTCATCGCTTGGCCTGTTTGCCGGAGTACTTGCGATGCGCCGCCGGGGCGGCAT
GGTTGAAAATATTCAGGAACTGGGCGGCCTCGCGCGACGCCAGCCAGTCTTTGCAATCGCGATGACCGTTTTGGTGCTCT
CGGTTGCGGGATTGCCTCCGTTCGGCGGCTTCTTCGGCAAGTATGAGGTGCTGCGCGCAGGTGTCGAAGCAGAGCTCGTG
CCATTGGCTATCATTCTGGTGATCGCTTCCGTTGTGTCCTTGGGGTATTATCTCCGCTTGCTGAAAGTCATGTGGTTCGA
TGAACCCAAAGAGACTTTTGAGCGTGTGGATGTGACGGTTCTGGGCACCGTTGTCGCTTGCGCCGTGATCGCCGGTGTTG
TGTTCATGATCTTTATCGGGGAACTCGGCACGGGTGGTTGGGCCAGCTATGCTGCTTCGGGACTTCTCCCTTGA

Upstream 100 bases:

>100_bases
CTCACAGTTCTTTTTGGTGTTGCCCCCAACCTCATTTTCGCCGTCACCAATGGTGCTACAGGGCGCATTATCGCGCTCAT
GGCGGGAGGCCAGTAAGCCG

Downstream 100 bases:

>100_bases
GCGCTTCCTGGCCACTCTACCGGTTCGAAGAAATTGACAGCACCAACACAGAAGCGAAGCGCCGCGCGGCTGAGGGCAGC
TTCAATAATCAATGGATTGT

Product: NADH-quinone oxidoreductase subunit N

Products: NA

Alternate protein names: NADH dehydrogenase I subunit N; NDH-1 subunit N

Number of amino acids: Translated: 477; Mature: 477

Protein sequence:

>477_residues
MLDTQSIIQAMAPELWLAASGLMLVLVGAIVGERFNSLSYKVGALVMFGAAALSAMHLQGGEAFNGLVKTTTFTNFTKVI
SFTLAGVAIFLSEGFLRRHEISRYEYPLLMVLASLGMGIILSANNLMTLYMGIETLSLSSYVLAAFHRDSARSSEAGLKY
FVLGALASGILLYGASLVYGFTGTTSFDGLTLAGESVGAMFGMVLMIVGLAFKISAAPMHVWTPDVYEGAPTPVVAFFAS
APKFASLVVFANVMFTLFGSVMDQWQLIVAIIAGLSMIVGSLGGLTQNNIKRLLAYSSIANVGYALVAVAAGQVLGAPAL
LTFSTIYVISSLGLFAGVLAMRRRGGMVENIQELGGLARRQPVFAIAMTVLVLSVAGLPPFGGFFGKYEVLRAGVEAELV
PLAIILVIASVVSLGYYLRLLKVMWFDEPKETFERVDVTVLGTVVACAVIAGVVFMIFIGELGTGGWASYAASGLLP

Sequences:

>Translated_477_residues
MLDTQSIIQAMAPELWLAASGLMLVLVGAIVGERFNSLSYKVGALVMFGAAALSAMHLQGGEAFNGLVKTTTFTNFTKVI
SFTLAGVAIFLSEGFLRRHEISRYEYPLLMVLASLGMGIILSANNLMTLYMGIETLSLSSYVLAAFHRDSARSSEAGLKY
FVLGALASGILLYGASLVYGFTGTTSFDGLTLAGESVGAMFGMVLMIVGLAFKISAAPMHVWTPDVYEGAPTPVVAFFAS
APKFASLVVFANVMFTLFGSVMDQWQLIVAIIAGLSMIVGSLGGLTQNNIKRLLAYSSIANVGYALVAVAAGQVLGAPAL
LTFSTIYVISSLGLFAGVLAMRRRGGMVENIQELGGLARRQPVFAIAMTVLVLSVAGLPPFGGFFGKYEVLRAGVEAELV
PLAIILVIASVVSLGYYLRLLKVMWFDEPKETFERVDVTVLGTVVACAVIAGVVFMIFIGELGTGGWASYAASGLLP
>Mature_477_residues
MLDTQSIIQAMAPELWLAASGLMLVLVGAIVGERFNSLSYKVGALVMFGAAALSAMHLQGGEAFNGLVKTTTFTNFTKVI
SFTLAGVAIFLSEGFLRRHEISRYEYPLLMVLASLGMGIILSANNLMTLYMGIETLSLSSYVLAAFHRDSARSSEAGLKY
FVLGALASGILLYGASLVYGFTGTTSFDGLTLAGESVGAMFGMVLMIVGLAFKISAAPMHVWTPDVYEGAPTPVVAFFAS
APKFASLVVFANVMFTLFGSVMDQWQLIVAIIAGLSMIVGSLGGLTQNNIKRLLAYSSIANVGYALVAVAAGQVLGAPAL
LTFSTIYVISSLGLFAGVLAMRRRGGMVENIQELGGLARRQPVFAIAMTVLVLSVAGLPPFGGFFGKYEVLRAGVEAELV
PLAIILVIASVVSLGYYLRLLKVMWFDEPKETFERVDVTVLGTVVACAVIAGVVFMIFIGELGTGGWASYAASGLLP

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1007

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 2 (chain N)

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 2 family

Homologues:

Organism=Homo sapiens, GI251831117, Length=328, Percent_Identity=23.1707317073171, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI145693160, Length=352, Percent_Identity=40.3409090909091, Blast_Score=202, Evalue=4e-53,
Organism=Escherichia coli, GI1788831, Length=319, Percent_Identity=26.9592476489028, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1788614, Length=326, Percent_Identity=23.6196319018405, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1788829, Length=398, Percent_Identity=27.6381909547739, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1788827, Length=360, Percent_Identity=26.6666666666667, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI2367154, Length=332, Percent_Identity=25, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1788613, Length=348, Percent_Identity=24.7126436781609, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NUON_HYPNA (Q0C1D1)

Other databases:

- EMBL:   CP000158
- RefSeq:   YP_760462.1
- STRING:   Q0C1D1
- GeneID:   4289254
- GenomeReviews:   CP000158_GR
- KEGG:   hne:HNE_1758
- NMPDR:   fig|228405.5.peg.1693
- TIGR:   HNE_1758
- HOGENOM:   HBG747830
- OMA:   ILMTITI
- PhylomeDB:   Q0C1D1
- ProtClustDB:   CLSK2532140
- BioCyc:   HNEP81032:HNE_1758-MONOMER
- GO:   GO:0006810
- HAMAP:   MF_00445
- InterPro:   IPR010096
- InterPro:   IPR001750
- TIGRFAMs:   TIGR01770

Pfam domain/function: PF00361 Oxidored_q1

EC number: =1.6.99.5

Molecular weight: Translated: 50334; Mature: 50334

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x13de2b24)-; HASH(0x13116d54)-; HASH(0x13e588bc)-; HASH(0x13b2b6f8)-; HASH(0x139e534c)-; HASH(0x1346b434)-; HASH(0x13b59f2c)-; HASH(0x13a0b5d0)-; HASH(0x13116ebc)-; HASH(0x1306b9d4)-; HASH(0x139b4270)-; HASH(0x13116e74)-; HASH(0x139b43b4)-;

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDTQSIIQAMAPELWLAASGLMLVLVGAIVGERFNSLSYKVGALVMFGAAALSAMHLQG
CCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GEAFNGLVKTTTFTNFTKVISFTLAGVAIFLSEGFLRRHEISRYEYPLLMVLASLGMGII
CHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCEE
LSANNLMTLYMGIETLSLSSYVLAAFHRDSARSSEAGLKYFVLGALASGILLYGASLVYG
EECCCEEEEEECHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEE
FTGTTSFDGLTLAGESVGAMFGMVLMIVGLAFKISAAPMHVWTPDVYEGAPTPVVAFFAS
CCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCHHHHHHHC
APKFASLVVFANVMFTLFGSVMDQWQLIVAIIAGLSMIVGSLGGLTQNNIKRLLAYSSIA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
NVGYALVAVAAGQVLGAPALLTFSTIYVISSLGLFAGVLAMRRRGGMVENIQELGGLARR
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHH
QPVFAIAMTVLVLSVAGLPPFGGFFGKYEVLRAGVEAELVPLAIILVIASVVSLGYYLRL
CHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LKVMWFDEPKETFERVDVTVLGTVVACAVIAGVVFMIFIGELGTGGWASYAASGLLP
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCH
>Mature Secondary Structure
MLDTQSIIQAMAPELWLAASGLMLVLVGAIVGERFNSLSYKVGALVMFGAAALSAMHLQG
CCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GEAFNGLVKTTTFTNFTKVISFTLAGVAIFLSEGFLRRHEISRYEYPLLMVLASLGMGII
CHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCEE
LSANNLMTLYMGIETLSLSSYVLAAFHRDSARSSEAGLKYFVLGALASGILLYGASLVYG
EECCCEEEEEECHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEE
FTGTTSFDGLTLAGESVGAMFGMVLMIVGLAFKISAAPMHVWTPDVYEGAPTPVVAFFAS
CCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCHHHHHHHC
APKFASLVVFANVMFTLFGSVMDQWQLIVAIIAGLSMIVGSLGGLTQNNIKRLLAYSSIA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
NVGYALVAVAAGQVLGAPALLTFSTIYVISSLGLFAGVLAMRRRGGMVENIQELGGLARR
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHH
QPVFAIAMTVLVLSVAGLPPFGGFFGKYEVLRAGVEAELVPLAIILVIASVVSLGYYLRL
CHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LKVMWFDEPKETFERVDVTVLGTVVACAVIAGVVFMIFIGELGTGGWASYAASGLLP
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA