Click here to switch to the map view.

The map label for this gene is trmE

Identifier: 114799625

GI number: 114799625

Start: 3699586

End: 3700911

Strand: Reverse

Name: trmE

Synonym: HNE_3564

Alternate gene names: 114799625

Gene position: 3700911-3699586 (Counterclockwise)

Preceding gene: 114798602

Following gene: 114797761

Centisome position: 99.89

GC content: 63.2

Gene sequence:

>1326_bases
ATGGCCGCGCGCGACACAATCTGCGCTCTGGCTTCCGGCCCGCCGCCCTCGGCCATTGCCATTATCCGGATTTCCGGTCC
CGCAGTTGCGGAGATCGGAAAGTCGCTTTTGGCGTCAGGCCTGCCCGAACCCAAACGCGCGGCGCTGACATATATATATG
ACTCTGATGGCGCGCTGATTGACCAGGGCATCGCACTCTATGCCAACGCGCCCCATTCCTATACGGGCGAAGACACGCTT
GAACTCTACCTTCATGGCGGACCGGCGGTCATAGACCATGCCCTCCGTGGGCTGACGGCCATAGCTGGCGTGCGCCTGGC
AGAGCCTGGAGAGTTCACCCGCCGCGCGTTTGAAAATGGCAAACTCGACCTCACCGAGGCTGAAGGTGTCGCCGACATCA
TCGAGGCGGAAACCGGTGCCCAGAAGGCGCAGGCACTCCGCCAGCTCGGAGGCGGGCTCACCGAAATTTACGATGGCTGG
CGGGAGGAACTGACCGGCACGCTCGCCCTTATCGAAGTGATGATCGACTTCCCCGATGAGGGCGATGTGCCCGAGGACAC
CGCGCGGCCCATCCTCTCTCGGCTCGAAAAGATCATGAGCGAAATTGAAACGGCGTTGGGCGACCGGGGTGTCGGTGAGC
GTATCCGGGACGGATTCCGCGTCGCAATTGTCGGCCCACCAAATGCCGGCAAGTCCTCCATCCTCAATCGCCTCGCCCGT
CGCGAAGCCGCCATTGTCACGGACATCGCCGGCACTACGCGCGACGTTGTCGAGGTCCGGCTCGTCCTGGGCGGACAGGT
TGTCTGGATCGCCGACACGGCGGGTCTGCGTGAGACCGAAGACGTGGTTGAGGCAGAAGGTGTTCGCCGGGCGCGCCGCG
CCGCTGCGGAGGCTGATCTGCGTATCCATGTCATCGATGGGACAGACCCCTCCCCTCCCCCGGAGCAGGTCGAGACCCAG
GACATCATCGTGTTCAACAAGGCCGATCAACGCCCGGCAATTCTTGCGCCCGATGGCGCGCTGCCCATTTCGGTCATAAC
GGGCGAAGGTATCGATAAACTAGAATCCTGGATCGCCGCGTTCGTATCCCGGCGGGCCTCCTCGGTCGAAGCGCCGGTCA
TCACGCGGGCGCGTCACCGCGAAAAGCTGGTTGCCGGGCTGGCAAGTCTGACGGCGGCGCGTGACGCGCTGGTTTCGGAC
ATGGGCGCCGAACTTGCCGGCGAAGACGTTCGCATGGCGCTTCGCCAGCTCTCCTCCGTCATCGGCCGTGTCGATGTAGA
GGATGTTCTGGGCGCGGTCTTTTCCAAATTCTGCATCGGAAAATGA

Upstream 100 bases:

>100_bases
GGGCGCCAACGATGCCGTCGACTTCCTGCTCGACAAGCTGAAGCAGACGAAGACGAACGACGAATTCTTCGAAGCGATGA
AGAACTAGAGGCTGGGCCGG

Downstream 100 bases:

>100_bases
CCCCTAGGGTTTGAAGGCCTCCGGCGTTATATACCCGCCTCAAGCAGCGCTTAAGGGTTGGTTAATGCCGGACTTCCCGG
ATTTCGACGTAATAGTCATT

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 441; Mature: 440

Protein sequence:

>441_residues
MAARDTICALASGPPPSAIAIIRISGPAVAEIGKSLLASGLPEPKRAALTYIYDSDGALIDQGIALYANAPHSYTGEDTL
ELYLHGGPAVIDHALRGLTAIAGVRLAEPGEFTRRAFENGKLDLTEAEGVADIIEAETGAQKAQALRQLGGGLTEIYDGW
REELTGTLALIEVMIDFPDEGDVPEDTARPILSRLEKIMSEIETALGDRGVGERIRDGFRVAIVGPPNAGKSSILNRLAR
REAAIVTDIAGTTRDVVEVRLVLGGQVVWIADTAGLRETEDVVEAEGVRRARRAAAEADLRIHVIDGTDPSPPPEQVETQ
DIIVFNKADQRPAILAPDGALPISVITGEGIDKLESWIAAFVSRRASSVEAPVITRARHREKLVAGLASLTAARDALVSD
MGAELAGEDVRMALRQLSSVIGRVDVEDVLGAVFSKFCIGK

Sequences:

>Translated_441_residues
MAARDTICALASGPPPSAIAIIRISGPAVAEIGKSLLASGLPEPKRAALTYIYDSDGALIDQGIALYANAPHSYTGEDTL
ELYLHGGPAVIDHALRGLTAIAGVRLAEPGEFTRRAFENGKLDLTEAEGVADIIEAETGAQKAQALRQLGGGLTEIYDGW
REELTGTLALIEVMIDFPDEGDVPEDTARPILSRLEKIMSEIETALGDRGVGERIRDGFRVAIVGPPNAGKSSILNRLAR
REAAIVTDIAGTTRDVVEVRLVLGGQVVWIADTAGLRETEDVVEAEGVRRARRAAAEADLRIHVIDGTDPSPPPEQVETQ
DIIVFNKADQRPAILAPDGALPISVITGEGIDKLESWIAAFVSRRASSVEAPVITRARHREKLVAGLASLTAARDALVSD
MGAELAGEDVRMALRQLSSVIGRVDVEDVLGAVFSKFCIGK
>Mature_440_residues
AARDTICALASGPPPSAIAIIRISGPAVAEIGKSLLASGLPEPKRAALTYIYDSDGALIDQGIALYANAPHSYTGEDTLE
LYLHGGPAVIDHALRGLTAIAGVRLAEPGEFTRRAFENGKLDLTEAEGVADIIEAETGAQKAQALRQLGGGLTEIYDGWR
EELTGTLALIEVMIDFPDEGDVPEDTARPILSRLEKIMSEIETALGDRGVGERIRDGFRVAIVGPPNAGKSSILNRLARR
EAAIVTDIAGTTRDVVEVRLVLGGQVVWIADTAGLRETEDVVEAEGVRRARRAAAEADLRIHVIDGTDPSPPPEQVETQD
IIVFNKADQRPAILAPDGALPISVITGEGIDKLESWIAAFVSRRASSVEAPVITRARHREKLVAGLASLTAARDALVSDM
GAELAGEDVRMALRQLSSVIGRVDVEDVLGAVFSKFCIGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain

Homologues:

Organism=Homo sapiens, GI193082991, Length=468, Percent_Identity=39.3162393162393, Blast_Score=278, Evalue=9e-75,
Organism=Homo sapiens, GI193082993, Length=451, Percent_Identity=39.689578713969, Blast_Score=272, Evalue=5e-73,
Organism=Homo sapiens, GI193082988, Length=496, Percent_Identity=37.2983870967742, Blast_Score=263, Evalue=2e-70,
Organism=Escherichia coli, GI2367268, Length=457, Percent_Identity=38.2932166301969, Blast_Score=274, Evalue=1e-74,
Organism=Caenorhabditis elegans, GI17507259, Length=451, Percent_Identity=34.1463414634146, Blast_Score=220, Evalue=1e-57,
Organism=Saccharomyces cerevisiae, GI6323665, Length=492, Percent_Identity=34.1463414634146, Blast_Score=247, Evalue=2e-66,
Organism=Drosophila melanogaster, GI45550813, Length=472, Percent_Identity=35.3813559322034, Blast_Score=257, Evalue=1e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNME_HYPNA (Q0BWA8)

Other databases:

- EMBL:   CP000158
- RefSeq:   YP_762235.1
- ProteinModelPortal:   Q0BWA8
- SMR:   Q0BWA8
- STRING:   Q0BWA8
- GeneID:   4290265
- GenomeReviews:   CP000158_GR
- KEGG:   hne:HNE_3564
- NMPDR:   fig|228405.5.peg.3413
- TIGR:   HNE_3564
- eggNOG:   COG0486
- HOGENOM:   HBG746795
- OMA:   KGPNSFT
- PhylomeDB:   Q0BWA8
- BioCyc:   HNEP81032:HNE_3564-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00379
- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520
- Gene3D:   G3DSA:3.30.1360.120
- TIGRFAMs:   TIGR00450
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N

EC number: NA

Molecular weight: Translated: 46761; Mature: 46629

Theoretical pI: Translated: 4.53; Mature: 4.53

Prosite motif: NA

Important sites: BINDING 23-23 BINDING 81-81 BINDING 121-121 BINDING 441-441

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAARDTICALASGPPPSAIAIIRISGPAVAEIGKSLLASGLPEPKRAALTYIYDSDGALI
CCCCCCEEEECCCCCCCEEEEEEECCCHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCEE
DQGIALYANAPHSYTGEDTLELYLHGGPAVIDHALRGLTAIAGVRLAEPGEFTRRAFENG
CCCEEEEECCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHCCEECCCCHHHHHHHCCC
KLDLTEAEGVADIIEAETGAQKAQALRQLGGGLTEIYDGWREELTGTLALIEVMIDFPDE
CEEEEHHCCHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCC
GDVPEDTARPILSRLEKIMSEIETALGDRGVGERIRDGFRVAIVGPPNAGKSSILNRLAR
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCEEEEEECCCCCCHHHHHHHHHH
REAAIVTDIAGTTRDVVEVRLVLGGQVVWIADTAGLRETEDVVEAEGVRRARRAAAEADL
HHHHHHHHCCCCHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCE
RIHVIDGTDPSPPPEQVETQDIIVFNKADQRPAILAPDGALPISVITGEGIDKLESWIAA
EEEEEECCCCCCCCCCCCCCEEEEEECCCCCCEEECCCCCCEEEEEECCCHHHHHHHHHH
FVSRRASSVEAPVITRARHREKLVAGLASLTAARDALVSDMGAELAGEDVRMALRQLSSV
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
IGRVDVEDVLGAVFSKFCIGK
HCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AARDTICALASGPPPSAIAIIRISGPAVAEIGKSLLASGLPEPKRAALTYIYDSDGALI
CCCCCEEEECCCCCCCEEEEEEECCCHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCEE
DQGIALYANAPHSYTGEDTLELYLHGGPAVIDHALRGLTAIAGVRLAEPGEFTRRAFENG
CCCEEEEECCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHCCEECCCCHHHHHHHCCC
KLDLTEAEGVADIIEAETGAQKAQALRQLGGGLTEIYDGWREELTGTLALIEVMIDFPDE
CEEEEHHCCHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCC
GDVPEDTARPILSRLEKIMSEIETALGDRGVGERIRDGFRVAIVGPPNAGKSSILNRLAR
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCEEEEEECCCCCCHHHHHHHHHH
REAAIVTDIAGTTRDVVEVRLVLGGQVVWIADTAGLRETEDVVEAEGVRRARRAAAEADL
HHHHHHHHCCCCHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCE
RIHVIDGTDPSPPPEQVETQDIIVFNKADQRPAILAPDGALPISVITGEGIDKLESWIAA
EEEEEECCCCCCCCCCCCCCEEEEEECCCCCCEEECCCCCCEEEEEECCCHHHHHHHHHH
FVSRRASSVEAPVITRARHREKLVAGLASLTAARDALVSDMGAELAGEDVRMALRQLSSV
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
IGRVDVEDVLGAVFSKFCIGK
HCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA