Click here to switch to the map view.

The map label for this gene is yngF [H]

Identifier: 114799454

GI number: 114799454

Start: 923676

End: 924758

Strand: Direct

Name: yngF [H]

Synonym: HNE_0907

Alternate gene names: 114799454

Gene position: 923676-924758 (Clockwise)

Preceding gene: 114797498

Following gene: 114800500

Centisome position: 24.93

GC content: 63.9

Gene sequence:

>1083_bases
TTGAGACCTGAGGAACACAGCATGAGTGCAGATGTAAGCATCAAGGTTACTGGCAATATCGGCCGGATCACGCTCACTCG
TCCTGCCGCTTTGCATGCCCTCAACACCCCGATGTGCGCTGAAATCCTCGCCGCCCTCCAGGCCTGGAAAGCAGACTCCG
CCATCCATCTTGTCTGGATAGATCATCAGGAAGGTACCCGCGGCTTCTGCTCGGGCGGCGACATTCGCATGCTTGCCGAA
AGCGGCGCCGGCGATGCCTCGGAGGCGCGCGACTTCTTCCGCACCGAATACCGCATGAACGCCGCCCTGAAGGCGTTCCC
CAAGCCTGTCCTCGCCATCCTCGATGGCGTCACCATGGGCGGCGGCGTCGGCCTCTCTGTCCACGGCTCCCACCGCGTGG
CCACCGAGCGCACCCTGTTCGCCATGCCCGAAACCGGTATCGGCCTGTTCCCGGATGTCGGCGGGGGCTGGTTCCTGCCG
CGCCTGGAAGGCCAGCTCGGCACATGGCTCGCCCTCACCGGCGCCCGCCTCAAGGGCGCCGATGTCGCTGCTGCGGGCGT
CGCCACGCATTATCTGCCCTCAGAGCTTGTCCCGGCGCTGGCCAAGCAGCTTGAAGCGGCAGACTTCTCGGCAGGCGCGG
CGGACATGCTGGACGAAATCCTCCGCCGTCTGACGCATCCGGTTCCGGCTCCGTCCTATGGCGCCCATAGCGAGACCATC
CATCGCTGCTTTGCAAAACAGACCGCTGAAGACATCGTCGCCGCCCTGGAAGGTGACGCCAGCGACTGGGCCAAGGCCGA
GGCGGCCACCATCCGCGCCAAATCTCCCGAGACAGTCAAGGTTGCCCTGCGCCAGCTGCGCGAAGGCGCCCGCTGTGAGA
CCTTTGAAGACAATATGCGCATGGAATTCCGCATCGGCTGGCGCAAGGTTCAAAGCCATGATTTCCAGGAAGGTGTGCGC
GCCGTCATTATAGATAAGGACAATGCGCCCGCCTGGTCGCCCGCGCGACTCGAAGAGGTCACCGAGGCAGATGTCGCGCG
ATACTTTGAAGCGCTGGGTGCGGACGAACTTACCTTCGCCTGA

Upstream 100 bases:

>100_bases
TTGGGCCATTTAGGGAATGGCATATCCTTATAATGACATACCCAAGTAAAGCCCTGTCCAAAGCCATTGATTTAATTGAA
CAGATTCCAGTCATCTGGAA

Downstream 100 bases:

>100_bases
TCTTAGGCGTTCAAGTATCAAGAGGAGACCGCCGCATGAACCCGGCCCTGATTACCTATGGCGCGCTCGGCATCGCTATC
ATGTTCGAGATCATCGGCAC

Product: enoyl-CoA hydratase/isomerase family protein

Products: trans(or 3)-enoyl-CoA; H2O [C]

Alternate protein names: NA

Number of amino acids: Translated: 360; Mature: 360

Protein sequence:

>360_residues
MRPEEHSMSADVSIKVTGNIGRITLTRPAALHALNTPMCAEILAALQAWKADSAIHLVWIDHQEGTRGFCSGGDIRMLAE
SGAGDASEARDFFRTEYRMNAALKAFPKPVLAILDGVTMGGGVGLSVHGSHRVATERTLFAMPETGIGLFPDVGGGWFLP
RLEGQLGTWLALTGARLKGADVAAAGVATHYLPSELVPALAKQLEAADFSAGAADMLDEILRRLTHPVPAPSYGAHSETI
HRCFAKQTAEDIVAALEGDASDWAKAEAATIRAKSPETVKVALRQLREGARCETFEDNMRMEFRIGWRKVQSHDFQEGVR
AVIIDKDNAPAWSPARLEEVTEADVARYFEALGADELTFA

Sequences:

>Translated_360_residues
MRPEEHSMSADVSIKVTGNIGRITLTRPAALHALNTPMCAEILAALQAWKADSAIHLVWIDHQEGTRGFCSGGDIRMLAE
SGAGDASEARDFFRTEYRMNAALKAFPKPVLAILDGVTMGGGVGLSVHGSHRVATERTLFAMPETGIGLFPDVGGGWFLP
RLEGQLGTWLALTGARLKGADVAAAGVATHYLPSELVPALAKQLEAADFSAGAADMLDEILRRLTHPVPAPSYGAHSETI
HRCFAKQTAEDIVAALEGDASDWAKAEAATIRAKSPETVKVALRQLREGARCETFEDNMRMEFRIGWRKVQSHDFQEGVR
AVIIDKDNAPAWSPARLEEVTEADVARYFEALGADELTFA
>Mature_360_residues
MRPEEHSMSADVSIKVTGNIGRITLTRPAALHALNTPMCAEILAALQAWKADSAIHLVWIDHQEGTRGFCSGGDIRMLAE
SGAGDASEARDFFRTEYRMNAALKAFPKPVLAILDGVTMGGGVGLSVHGSHRVATERTLFAMPETGIGLFPDVGGGWFLP
RLEGQLGTWLALTGARLKGADVAAAGVATHYLPSELVPALAKQLEAADFSAGAADMLDEILRRLTHPVPAPSYGAHSETI
HRCFAKQTAEDIVAALEGDASDWAKAEAATIRAKSPETVKVALRQLREGARCETFEDNMRMEFRIGWRKVQSHDFQEGVR
AVIIDKDNAPAWSPARLEEVTEADVARYFEALGADELTFA

Specific function: Could Possibly Oxidizes Fatty Acids Using Specific Components (By Similarity). [C]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI37594471, Length=365, Percent_Identity=43.5616438356164, Blast_Score=303, Evalue=2e-82,
Organism=Homo sapiens, GI37594469, Length=311, Percent_Identity=42.1221864951768, Blast_Score=241, Evalue=7e-64,
Organism=Homo sapiens, GI194097323, Length=180, Percent_Identity=30, Blast_Score=77, Evalue=2e-14,
Organism=Escherichia coli, GI1787659, Length=183, Percent_Identity=31.1475409836066, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1788682, Length=184, Percent_Identity=26.6304347826087, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI221142681, Length=171, Percent_Identity=29.2397660818713, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI25144157, Length=349, Percent_Identity=41.5472779369628, Blast_Score=277, Evalue=7e-75,
Organism=Caenorhabditis elegans, GI25144160, Length=349, Percent_Identity=41.5472779369628, Blast_Score=277, Evalue=8e-75,
Organism=Caenorhabditis elegans, GI25145438, Length=179, Percent_Identity=29.608938547486, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17554946, Length=250, Percent_Identity=26.4, Blast_Score=71, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI25144276, Length=139, Percent_Identity=31.6546762589928, Blast_Score=69, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17508951, Length=139, Percent_Identity=31.6546762589928, Blast_Score=69, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17508953, Length=165, Percent_Identity=28.4848484848485, Blast_Score=69, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17540714, Length=189, Percent_Identity=27.5132275132275, Blast_Score=68, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6320241, Length=338, Percent_Identity=33.1360946745562, Blast_Score=176, Evalue=6e-45,
Organism=Drosophila melanogaster, GI28571730, Length=345, Percent_Identity=45.2173913043478, Blast_Score=295, Evalue=4e-80,
Organism=Drosophila melanogaster, GI28571729, Length=345, Percent_Identity=45.2173913043478, Blast_Score=295, Evalue=4e-80,
Organism=Drosophila melanogaster, GI20129971, Length=178, Percent_Identity=27.5280898876405, Blast_Score=72, Evalue=7e-13,
Organism=Drosophila melanogaster, GI24653477, Length=178, Percent_Identity=27.5280898876405, Blast_Score=72, Evalue=7e-13,
Organism=Drosophila melanogaster, GI24653139, Length=175, Percent_Identity=28.5714285714286, Blast_Score=66, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: 4.2.1.17 [C]

Molecular weight: Translated: 38829; Mature: 38829

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPEEHSMSADVSIKVTGNIGRITLTRPAALHALNTPMCAEILAALQAWKADSAIHLVWI
CCCCCCCCCCCEEEEEECCCCEEEEECCHHHHHCCCHHHHHHHHHHHHHCCCCEEEEEEE
DHQEGTRGFCSGGDIRMLAESGAGDASEARDFFRTEYRMNAALKAFPKPVLAILDGVTMG
ECCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCEEC
GGVGLSVHGSHRVATERTLFAMPETGIGLFPDVGGGWFLPRLEGQLGTWLALTGARLKGA
CCEEEEECCCCCEECCCEEEECCCCCCCCCCCCCCCEECCCCCCCCCCEEEEECCCCCCC
DVAAAGVATHYLPSELVPALAKQLEAADFSAGAADMLDEILRRLTHPVPAPSYGAHSETI
CHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHH
HRCFAKQTAEDIVAALEGDASDWAKAEAATIRAKSPETVKVALRQLREGARCETFEDNMR
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHEEECCCCHHHHHHHHHHHCCCCCCCCCCCCE
MEFRIGWRKVQSHDFQEGVRAVIIDKDNAPAWSPARLEEVTEADVARYFEALGADELTFA
EEEEECHHHHHCCCHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MRPEEHSMSADVSIKVTGNIGRITLTRPAALHALNTPMCAEILAALQAWKADSAIHLVWI
CCCCCCCCCCCEEEEEECCCCEEEEECCHHHHHCCCHHHHHHHHHHHHHCCCCEEEEEEE
DHQEGTRGFCSGGDIRMLAESGAGDASEARDFFRTEYRMNAALKAFPKPVLAILDGVTMG
ECCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCEEC
GGVGLSVHGSHRVATERTLFAMPETGIGLFPDVGGGWFLPRLEGQLGTWLALTGARLKGA
CCEEEEECCCCCEECCCEEEECCCCCCCCCCCCCCCEECCCCCCCCCCEEEEECCCCCCC
DVAAAGVATHYLPSELVPALAKQLEAADFSAGAADMLDEILRRLTHPVPAPSYGAHSETI
CHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHH
HRCFAKQTAEDIVAALEGDASDWAKAEAATIRAKSPETVKVALRQLREGARCETFEDNMR
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHEEECCCCHHHHHHHHHHHCCCCCCCCCCCCE
MEFRIGWRKVQSHDFQEGVRAVIIDKDNAPAWSPARLEEVTEADVARYFEALGADELTFA
EEEEECHHHHHCCCHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.05 {crotonyl-CoA}} 0.008 {2-decenoyl-CoA}} [C]

Substrates: (3S)-hydroxyacyl-CoA [C]

Specific reaction: (3S)-hydroxyacyl-CoA = trans(or 3)-enoyl-CoA + H2O [C]

General reaction: addition (of H2O to carbon carbon double bondelimination (of H2O C-O bond cleavagehydration [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9387222; 9384377 [H]