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The map label for this gene is emrE [H]

Identifier: 114800500

GI number: 114800500

Start: 924794

End: 925138

Strand: Direct

Name: emrE [H]

Synonym: HNE_0908

Alternate gene names: 114800500

Gene position: 924794-925138 (Clockwise)

Preceding gene: 114799454

Following gene: 114797129

Centisome position: 24.96

GC content: 56.52

Gene sequence:

>345_bases
ATGAACCCGGCCCTGATTACCTATGGCGCGCTCGGCATCGCTATCATGTTCGAGATCATCGGCACAACGCTGCTTCAGCA
AAGCCAGCAGTTCACCCGCTGGCTGCCCACGCTCGGCATGGCGCTCTGCTATGGCGCGGCCTTTTACTTCCTCTCCATCG
CGCTGAAGACGATTCCCGTGGGCGTCGCTTATGCCATCTGGAGCGGCATGGGCATCGTTCTCATCGCGCTCATTGGCCTG
ATGCTGTTCGGCCAGAAACTGGACCTTCCAGCGATCATTGGTATGGCAATGATTATCGGCGGCGTCGTGGTAATGAATCT
ATTCTCTAAAACCGTGTCTCACTGA

Upstream 100 bases:

>100_bases
TCACCGAGGCAGATGTCGCGCGATACTTTGAAGCGCTGGGTGCGGACGAACTTACCTTCGCCTGATCTTAGGCGTTCAAG
TATCAAGAGGAGACCGCCGC

Downstream 100 bases:

>100_bases
GCTTAGCCTCAATATTTACATTTGGTTCATCAGTAGAAAGCCGCCCGGATACTTTCCTGGCGGCTTTTTTGTGCGTGTTG
TTTCAGAATGAAACGCATTT

Product: SMR family multidrug efflux pump

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Efflux-multidrug resistance protein emrE; Ethidium resistance protein; Methyl viologen resistance protein C [H]

Number of amino acids: Translated: 114; Mature: 114

Protein sequence:

>114_residues
MNPALITYGALGIAIMFEIIGTTLLQQSQQFTRWLPTLGMALCYGAAFYFLSIALKTIPVGVAYAIWSGMGIVLIALIGL
MLFGQKLDLPAIIGMAMIIGGVVVMNLFSKTVSH

Sequences:

>Translated_114_residues
MNPALITYGALGIAIMFEIIGTTLLQQSQQFTRWLPTLGMALCYGAAFYFLSIALKTIPVGVAYAIWSGMGIVLIALIGL
MLFGQKLDLPAIIGMAMIIGGVVVMNLFSKTVSH
>Mature_114_residues
MNPALITYGALGIAIMFEIIGTTLLQQSQQFTRWLPTLGMALCYGAAFYFLSIALKTIPVGVAYAIWSGMGIVLIALIGL
MLFGQKLDLPAIIGMAMIIGGVVVMNLFSKTVSH

Specific function: Multidrug transporter that expels positively charged hydrophobic drugs across the inner membrane of E.coli., thereby conferring resistance to a wide range of toxic compounds. The drug efflux is coupled to an influx of protons. Is involved in the resistanc

COG id: COG2076

COG function: function code P; Membrane transporters of cations and cationic drugs

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the small multidrug resistance (SMR) protein family [H]

Homologues:

Organism=Escherichia coli, GI1786755, Length=114, Percent_Identity=53.5087719298246, Blast_Score=118, Evalue=1e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000390 [H]

Pfam domain/function: PF00893 Multi_Drug_Res [H]

EC number: NA

Molecular weight: Translated: 12198; Mature: 12198

Theoretical pI: Translated: 9.11; Mature: 9.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
7.0 %Met     (Translated Protein)
7.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
7.0 %Met     (Mature Protein)
7.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPALITYGALGIAIMFEIIGTTLLQQSQQFTRWLPTLGMALCYGAAFYFLSIALKTIPV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GVAYAIWSGMGIVLIALIGLMLFGQKLDLPAIIGMAMIIGGVVVMNLFSKTVSH
HHHHHHHHCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNPALITYGALGIAIMFEIIGTTLLQQSQQFTRWLPTLGMALCYGAAFYFLSIALKTIPV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GVAYAIWSGMGIVLIALIGLMLFGQKLDLPAIIGMAMIIGGVVVMNLFSKTVSH
HHHHHHHHCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1936950; 1320256; 9278503; 7896833; 9050242; 9688273 [H]