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The map label for this gene is pcm [H]

Identifier: 114799434

GI number: 114799434

Start: 1725372

End: 1726028

Strand: Reverse

Name: pcm [H]

Synonym: HNE_1679

Alternate gene names: 114799434

Gene position: 1726028-1725372 (Counterclockwise)

Preceding gene: 114798376

Following gene: 114800241

Centisome position: 46.59

GC content: 64.54

Gene sequence:

>657_bases
ATGGACTTCGACCTAGCGCGCGAAATCATGGTGGACAGCCAGATCCGCCCGAATGATGTGACCGACCCGGCGATCGTGGG
CGCCTTCATGCGCACACCGCGCGAAGAATTCGTCCCCAGCGCGCGCCGCTCCGTCGCCTATTCGGAAATGGAAATCCAGA
CATCCGAAGGCCGCGCCCTGTGGACCCCGCGCGACACCGCCAAGCTGGTGAAGATGGCGCAGGTAAAACCCACCGATGTG
GTCCTCGTGATCGCGGCGGGCTCGGGCTATGAGGCCGCGCTGCTCAGCCATATCGCCGACACGGTCATCGCGCTGGATGA
TCAGCCGGGCCTGGTGGACGCCATGACCAGCCGTTTCGCGGACCTGGGCATTGATCGCATCGCGCCCGTGGAAGGCAAGA
TCGCCGAGGGTTTGCCCGCACAGGCGCCCTTCGACGTCATCTATGTCTGCGGCATGGTCGAAACGCTGCCCGAGGCCTGG
GGCGCACAGCTTGCTGAAGGTGGCCGCCTGGTGGCCGTGATGGCCGACGACAACCGCCGCCTTGGCCGCGGCAAGGTATT
CACGAAGGCCGGAGACACGCTGTCCTCACGCGATGGGTTTGACGCCTCTCCGCCAAAGCTCGCAGAATTTGATCGCAAGG
CCAGCTTCACCTTCTGA

Upstream 100 bases:

>100_bases
ACAGAAGAAGCATGTCGCGGCGATCCTGCGCATTGCGCGGGCGCGCCGTCATGCTAGAGGGGGCATACCGATCCAGCGAT
TCAGAAACGGCGATTTCAAG

Downstream 100 bases:

>100_bases
AAATGCCTGCGTCTTTATTGTTTGGTGAACAGCGTTCACCTTATCCCAATAAAGGCATATATATCCTGCTGTAAGGACCA
ACAGGGTATTCATCATGCGC

Product: putative protein-L-isoaspartate(D-aspartate) O-methyltransferase

Products: NA

Alternate protein names: L-isoaspartyl protein carboxyl methyltransferase; Protein L-isoaspartyl methyltransferase; Protein-beta-aspartate methyltransferase; PIMT [H]

Number of amino acids: Translated: 218; Mature: 218

Protein sequence:

>218_residues
MDFDLAREIMVDSQIRPNDVTDPAIVGAFMRTPREEFVPSARRSVAYSEMEIQTSEGRALWTPRDTAKLVKMAQVKPTDV
VLVIAAGSGYEAALLSHIADTVIALDDQPGLVDAMTSRFADLGIDRIAPVEGKIAEGLPAQAPFDVIYVCGMVETLPEAW
GAQLAEGGRLVAVMADDNRRLGRGKVFTKAGDTLSSRDGFDASPPKLAEFDRKASFTF

Sequences:

>Translated_218_residues
MDFDLAREIMVDSQIRPNDVTDPAIVGAFMRTPREEFVPSARRSVAYSEMEIQTSEGRALWTPRDTAKLVKMAQVKPTDV
VLVIAAGSGYEAALLSHIADTVIALDDQPGLVDAMTSRFADLGIDRIAPVEGKIAEGLPAQAPFDVIYVCGMVETLPEAW
GAQLAEGGRLVAVMADDNRRLGRGKVFTKAGDTLSSRDGFDASPPKLAEFDRKASFTF
>Mature_218_residues
MDFDLAREIMVDSQIRPNDVTDPAIVGAFMRTPREEFVPSARRSVAYSEMEIQTSEGRALWTPRDTAKLVKMAQVKPTDV
VLVIAAGSGYEAALLSHIADTVIALDDQPGLVDAMTSRFADLGIDRIAPVEGKIAEGLPAQAPFDVIYVCGMVETLPEAW
GAQLAEGGRLVAVMADDNRRLGRGKVFTKAGDTLSSRDGFDASPPKLAEFDRKASFTF

Specific function: Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins [H]

COG id: COG2518

COG function: function code O; Protein-L-isoaspartate carboxylmethyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. L- isoaspartyl/D-aspartyl protein methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789100, Length=170, Percent_Identity=29.4117647058824, Blast_Score=89, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000682 [H]

Pfam domain/function: PF01135 PCMT [H]

EC number: =2.1.1.77 [H]

Molecular weight: Translated: 23577; Mature: 23577

Theoretical pI: Translated: 4.49; Mature: 4.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFDLAREIMVDSQIRPNDVTDPAIVGAFMRTPREEFVPSARRSVAYSEMEIQTSEGRAL
CCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCHHHHCCHHHHHHHHHHEEEEECCCCEE
WTPRDTAKLVKMAQVKPTDVVLVIAAGSGYEAALLSHIADTVIALDDQPGLVDAMTSRFA
ECCCHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHEEEECCCCCHHHHHHHHHH
DLGIDRIAPVEGKIAEGLPAQAPFDVIYVCGMVETLPEAWGAQLAEGGRLVAVMADDNRR
HCCCCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCC
LGRGKVFTKAGDTLSSRDGFDASPPKLAEFDRKASFTF
CCCCEEEECCCCCCCCCCCCCCCCCHHHHHCCCCCCCC
>Mature Secondary Structure
MDFDLAREIMVDSQIRPNDVTDPAIVGAFMRTPREEFVPSARRSVAYSEMEIQTSEGRAL
CCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCHHHHCCHHHHHHHHHHEEEEECCCCEE
WTPRDTAKLVKMAQVKPTDVVLVIAAGSGYEAALLSHIADTVIALDDQPGLVDAMTSRFA
ECCCHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHEEEECCCCCHHHHHHHHHH
DLGIDRIAPVEGKIAEGLPAQAPFDVIYVCGMVETLPEAWGAQLAEGGRLVAVMADDNRR
HCCCCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCC
LGRGKVFTKAGDTLSSRDGFDASPPKLAEFDRKASFTF
CCCCEEEECCCCCCCCCCCCCCCCCHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA